Article ; Online: Robust Protocol for the Synthesis of BSA Nanohydrogels by Inverse Nanoemulsion for Drug Delivery.
Langmuir : the ACS journal of surfaces and colloids
2023 Volume 39, Issue 23, Page(s) 8176–8185
Abstract: In a highly efficient and reproducible process, bovine serum albumin (BSA) nanogels are prepared from inverse nanoemulsions. The concept of independent nanoreactors of the individual droplets in the nanoemulsions allows high protein concentrations of up ... ...
| Abstract | In a highly efficient and reproducible process, bovine serum albumin (BSA) nanogels are prepared from inverse nanoemulsions. The concept of independent nanoreactors of the individual droplets in the nanoemulsions allows high protein concentrations of up to 0.6% in the inverse total system. The BSA gel networks are generated by the 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride coupling strategy widely used in protein chemistry. In a robust work-up protocol, the hydrophobic continuous phase of the inverse emulsion is stepwise replaced by water without compromising the colloidal stability and non-toxicity of the nanogel particles. Further, the simple process allows the loading of the nanogels with various cargos like a dye (Dy-495), a drug (ibuprofen), another protein [FMN-binding fluorescent protein (EcFbFP)], and oligonucleotides [plasmid DNA for enhanced GFP expression in mammalian cells (pEGFP c3) and a synthetic anti- |
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| MeSH term(s) | Rats ; Animals ; Humans ; Nanogels ; Serum Albumin, Bovine/chemistry ; Drug Delivery Systems ; Ibuprofen ; Cell Line ; Drug Carriers/chemistry ; Mammals |
| Chemical Substances | Nanogels ; Serum Albumin, Bovine (27432CM55Q) ; Ibuprofen (WK2XYI10QM) ; Drug Carriers |
| Language | English |
| Publishing date | 2023-05-29 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2005937-1 |
| ISSN | 1520-5827 ; 0743-7463 |
| ISSN (online) | 1520-5827 |
| ISSN | 0743-7463 |
| DOI | 10.1021/acs.langmuir.3c00635 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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