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Article ; Online: Curva de crescimento e primeira maturação sexual do mandi Pimeludos maculatus Lac. 1803 (Pisces, Siluroidei)

N.A. Fenerich / M.Y. Narahara / H.M. Godinho

Boletim do Instituto de Pesca, Vol 4, Iss 4(1-4) (2018)

2018

Abstract: O objetivo do presente trabalho é estabelecer uma correlação entre os parí¢metros idade e comprimento para obter a curva de crescimento e determinar o comprimento e a idade em que se inicia a maturação sexual do mandi, Pimelodus maculatus. A idade foi ... ...

Abstract	O objetivo do presente trabalho é estabelecer uma correlação entre os parí¢metros idade e comprimento para obter a curva de crescimento e determinar o comprimento e a idade em que se inicia a maturação sexual do mandi, Pimelodus maculatus. A idade foi determinada a partir de anéis translúcidos encontrados nos otolitos lapilli, sendo constatado que sua formação é anual. Ajustando-se os comprimentos totais médios aos grupos de idade definidos pelo exame dos otolitos através da equação de von Bertalanffy, encontram-se: LâË†Å¾ = 56,5 cm k = 0,1938 para fêmeas LâË†Å¾ = 45,4 cm k = 0,2104 para machos A primeira maturação sexual se dá aos 18 e 19 cm, para machos e fêmeas, respectivamente, entre 1 ano e meio e 2 anos de idade.
Keywords	NíO CONSTA ; Aquaculture. Fisheries. Angling ; SH1-691 ; Environmental sciences ; GE1-350 ; Oceanography ; GC1-1581
Language	English
Publishing date	2018-06-01T00:00:00Z
Publisher	Instituto de Pesca
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Comparing extraction method efficiency for high-throughput palaeoproteomic bone species identification

Dorothea Mylopotamitaki / Florian S. Harking / Alberto J. Taurozzi / Zandra Fagernäs / Ricardo M. Godinho / Geoff M. Smith / Marcel Weiss / Tim Schüler / Shannon P. McPherron / Harald Meller / João Cascalheira / Nuno Bicho / Jesper V. Olsen / Jean-Jacques Hublin / Frido Welker

Scientific Reports, Vol 13, Iss 1, Pp 1-

2023 Volume 14

Abstract: Abstract High-throughput proteomic analysis of archaeological skeletal remains provides information about past fauna community compositions and species dispersals in time and space. Archaeological skeletal remains are a finite resource, however, and ... ...

Abstract	Abstract High-throughput proteomic analysis of archaeological skeletal remains provides information about past fauna community compositions and species dispersals in time and space. Archaeological skeletal remains are a finite resource, however, and therefore it becomes relevant to optimize methods of skeletal proteome extraction. Ancient proteins in bone specimens can be highly degraded and consequently, extraction methods for well-preserved or modern bone might be unsuitable for the processing of highly degraded skeletal proteomes. In this study, we compared six proteomic extraction methods on Late Pleistocene remains with variable levels of proteome preservation. We tested the accuracy of species identification, protein sequence coverage, deamidation, and the number of post-translational modifications per method. We find striking differences in obtained proteome complexity and sequence coverage, highlighting that simple acid-insoluble proteome extraction methods perform better in highly degraded contexts. For well-preserved specimens, the approach using EDTA demineralization and protease-mix proteolysis yielded a higher number of identified peptides. The protocols presented here allowed protein extraction from ancient bone with a minimum number of working steps and equipment and yielded protein extracts within three working days. We expect further development along this route to benefit large-scale screening applications of relevance to archaeological and human evolution research.
Keywords	Medicine ; R ; Science ; Q
Subject code	930
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Influência de fatores abióticos sobre a maturação dos ovários de Pimelodus maculatus Lac. 1803 (Pisces, Siluroidei)

M.A. Basile - Martins / H.M. Godinho / N.A. Fenerich / J.M.B. Barker

Boletim do Instituto de Pesca, Vol 4, Iss 4(1-4) (2018)

2018

Abstract: O presente trabalho teve o propósito de estabelecer correlações entre alguns fatores abióticos e a maturação dos ovários de P. maculatus, expressa por valores médios do índice gônado somático (IGS). Foi encontrada uma correlação positiva entre o IGS e ... ...

Abstract	O presente trabalho teve o propósito de estabelecer correlações entre alguns fatores abióticos e a maturação dos ovários de P. maculatus, expressa por valores médios do índice gônado somático (IGS). Foi encontrada uma correlação positiva entre o IGS e a precipitação pluviométrica. Foi observado também, que a maturação dos ovários depende da temperatura da água e do nível do rio.
Keywords	NíO CONSTA ; Aquaculture. Fisheries. Angling ; SH1-691 ; Environmental sciences ; GE1-350 ; Oceanography ; GC1-1581
Language	English
Publishing date	2018-06-01T00:00:00Z
Publisher	Instituto de Pesca
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Photochemical features and trace element substituted chlorophylls as early detection biomarkers of metal exposure in the model diatom Phaeodactylum tricornutum

Cabrita, Maria Teresa / Bernardo Duarte / Carla Gameiro / Rita M. Godinho / Isabel Caçador

Ecological indicators. 2018 Dec., v. 95

2018

Abstract: Establishing marine species equipped with efficient biomarkers of trace element stress is a major requirement for the assessment of trace element contamination in marine ecosystems. This study investigates PS II overall activity and efficiency during ... ...

Abstract	Establishing marine species equipped with efficient biomarkers of trace element stress is a major requirement for the assessment of trace element contamination in marine ecosystems. This study investigates PS II overall activity and efficiency during light harvesting, electron transport chain (ETC) behaviour, OJIP-transient light curves, and pigment profiles, in the model marine diatom species Phaeodactylum tricornutum exposed to Cr, Co, Ni, Cu, Zn, Cd, Hg, Pb and to a mixture of all elements combined (Mix). OJIP-test parameters and trace element substituted chlorophylls were investigated as potential biomarkers of stress induced by trace elements. Hg-exposed cells showed a shift in balance towards PS I activity, promoted by increase in the electron transport from PQH2 to the reduction of PS I end electron acceptors. Other elements had more limited effects, mostly affecting energy dissipation and pigment levels to various degrees. Decoupling of PS II antennae connectivity was found in Co exposed cells. Chromium induced negative effects on PS I, but had no influence at the Oxygen Evolving Complexes (OECs) level. Copper caused a shift in the equilibrium between PS towards the PS I by reducing the PS II efficiency. Cadmium induced damage in OECs of the PS II donor side, but had no effect on the energy transduction pathway, maintaining energy processing efficiency. Lead had no significant effect on the energy transduction pathway, but produced changes at pigment level, leading to an increase in the chlorophyll and diadinoxanthin. In contrast, Ni had a beneficial role in P. tricornutum photochemistry, leading to increased number of available RC and oxidized quinone pool size, possibly because concentrations used were not able to induce severe cell damage. Copper, Cd and Zn led to substitution of Mg in chlorophyll a molecules, with the formation of less efficient CuChl a, CdChl a and ZnChl a. The biomarkers used evidenced the damaging effect of Co, Cu, Cr, Pb, Hg and all trace elements combined (Mix), pointing to Hg as the most harmful element. These physiological changes highlight P. tricornutum as a promising sentinel species for trace element contamination and the proposed photochemical features suitable as an efficient battery of biomarkers for trace element stress early detection in marine systems.
Keywords	Phaeodactylum tricornutum ; biomarkers ; cadmium ; chlorophyll ; chromium ; cobalt ; copper ; electron transfer ; electron transport chain ; energy ; indicator species ; lead ; magnesium ; marine ecosystems ; mercury ; models ; nickel ; oxygen evolving complex ; photochemistry ; zinc
Language	English
Dates of publication	2018-12
Size	p. 1038-1052.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	2036774-0
ISSN	1872-7034 ; 1470-160X
ISSN (online)	1872-7034
ISSN	1470-160X
DOI	10.1016/j.ecolind.2017.07.057
Database	NAL-Catalogue (AGRICOLA)

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Article: Axial heterogeneities in capillary ultrahigh pressure liquid chromatography columns: Chromatographic and bed morphological characterization

Reising, Arved E / James W. Jorgenson / Janek Bernzen / Justin M. Godinho / Ulrich Tallarek

Journal of chromatography. 2018 Sept. 28, v. 1569

2018

Abstract: We study axial heterogeneities in capillary ultrahigh pressure liquid chromatography (UHPLC) columns through kinetic performance and bed morphological analysis. Two columns are used in this work, a 75 μm i.d. × 100 cm column packed with 1.3 μm C18-silica ...

Abstract	We study axial heterogeneities in capillary ultrahigh pressure liquid chromatography (UHPLC) columns through kinetic performance and bed morphological analysis. Two columns are used in this work, a 75 μm i.d. × 100 cm column packed with 1.3 μm C18-silica particles and a 75 μm i.d. × 45 cm column packed with 1.9 μm C18-silica particles. The long column is chromatographically characterized and is afterwards sectioned into three segments, each analyzed individually. The column packed with the 1.9 μm particles is subjected to a bed morphological analysis using confocal laser scanning microscopy near the inlet, center, and outlet of the column. Chromatographic and morphological characterizations reveal highest separation efficiency and most homogeneous bed microstructure towards the column outlet. Kinetic performance data for inlet and central packing segments indicate enhanced contributions from wall effects to a transcolumn flow heterogeneity. Bed morphological data reveal systematic changes in geometrical and frictional wall effects along the bed: from inlet to outlet, bed morphologies increasingly reflect packing microstructures associated with concentrated slurries. Variations in separation efficiency and bed morphology can be related to the constant-pressure packing mode; the decrease in packing rate along the bed leaves fewer chances for particle rearrangement and bed consolidation from inlet to outlet. It explains the relatively uniform bed morphology towards the outlet and also the relatively loose wall region near the inlet. Bed microstructural features are discussed in a context of previous observations made in the characterization of capillary UHPLC columns.
Keywords	confocal laser scanning microscopy ; microstructure ; slurries ; ultra-performance liquid chromatography
Language	English
Dates of publication	2018-0928
Size	p. 44-52.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	218139-3
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
DOI	10.1016/j.chroma.2018.07.037
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Hiperdontia

M. Godinho / A. Figueiredo / S. Macedo / A. Correia

Cadernos de Saúde, Vol 6, Iss Especial.

a propósito de um caso clínico

2013 Volume 1

Keywords	Medicine (General) ; R5-920 ; Public aspects of medicine ; RA1-1270
Language	English
Publishing date	2013-01-01T00:00:00Z
Publisher	Universidade Católica Portuguesa, Instituto de Ciências da Saúde
Document type	Article ; Online
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Article: Measuring porosities of chromatographic columns utilizing a mass-based total pore-blocking method: Superficially porous particles and pore-blocking critical pressure mechanism

Devitt, Nicole M / Robert E. Moran / Justin M. Godinho / Brian M. Wagner / Mark R. Schure

Journal of chromatography. 2019 June 21, v. 1595

2019

Abstract: Experimentally determined total, interstitial and intraparticle porosity values are necessary to equate theory, simulation and experimental column performance. This paper reports a study of a mass-based technique for determining total, interstitial and ... ...

Abstract	Experimentally determined total, interstitial and intraparticle porosity values are necessary to equate theory, simulation and experimental column performance. This paper reports a study of a mass-based technique for determining total, interstitial and intraparticle porosity measurements based on the total pore-blocking (TPB) method. Commercially available superficially porous particle (SPP) columns, in a variety of small-pore and wide-pore materials, with both hydrophobic and hydrophilic surfaces, are utilized as samples. The results are compared with previously determined literature values for a number of columns and contrasted with HPLC-based elution methods. This method uses only a high-precision balance and an HPLC pump.A simple theoretical analysis of the TPB method using the Young-Laplace equation shows the pressure bounds and flow rate constraints of the method which ensure pore blocking stability. The results suggest that particles with small-pore diameters can be analyzed over a range of solvent clearing pressures and flow rates. However, wide-pore materials, typically with pore diameters in excess of 400 Å, have very low critical pressures and are difficult to determine without losing the pore blocking component. Small mass differences between clearing solvents are shown to present a challenge for measuring the interstitial volume.
Keywords	equations ; high performance liquid chromatography ; hydrophilicity ; hydrophobicity ; porosity ; solvents
Language	English
Dates of publication	2019-0621
Size	p. 117-126.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	218139-3
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
DOI	10.1016/j.chroma.2019.02.045
Database	NAL-Catalogue (AGRICOLA)

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Article: Bed morphological features associated with an optimal slurry concentration for reproducible preparation of efficient capillary ultrahigh pressure liquid chromatography columns

Reising, Arved E / James W. Jorgenson / Justin M. Godinho / Ulrich Tallarek

Journal of chromatography. 2017,

2017

Abstract: Column wall effects and the formation of larger voids in the bed during column packing are factors limiting the achievement of highly efficient columns. Systematic variation of packing conditions, combined with three-dimensional bed reconstruction and ... ...

Abstract	Column wall effects and the formation of larger voids in the bed during column packing are factors limiting the achievement of highly efficient columns. Systematic variation of packing conditions, combined with three-dimensional bed reconstruction and detailed morphological analysis of column beds, provide valuable insights into the packing process. Here, we study a set of sixteen 75Î¼m i.d. fused-silica capillary columns packed with 1.9Î¼m, C18-modified, bridged-ethyl hybrid silica particles slurried in acetone to concentrations ranging from 5 to 200mg/mL. Bed reconstructions for three of these columns (representing low, optimal, and high slurry concentrations), based on confocal laser scanning microscopy, reveal morphological features associated with the implemented slurry concentration, that lead to differences in column efficiency. At a low slurry concentration, the bed microstructure includes systematic radial heterogeneities such as particle size-segregation and local deviations from bulk packing density near the wall. These effects are suppressed (or at least reduced) with higher slurry concentrations. Concomitantly, larger voids (relative to the mean particle diameter) begin to form in the packing and increase in size and number with the slurry concentration. The most efficient columns are packed at slurry concentrations that balance these counteracting effects. Videos are taken at low and high slurry concentration to elucidate the bed formation process. At low slurry concentrations, particles arrive and settle individually, allowing for rearrangements. At high slurry concentrations they arrive and pack as large patches (reflecting particle aggregation in the slurry). These processes are discussed with respect to column packing, chromatographic performance, and bed microstructure to help reinforce general trends previously described. Conclusions based on this comprehensive analysis guide us towards further improvement of the packing process.
Keywords	acetone ; confocal laser scanning microscopy ; liquid chromatography ; microstructure ; silica ; slurries
Language	English
Size	p. .
Publishing place	Elsevier B.V.
Document type	Article
Note	Pre-press version
ZDB-ID	218139-3
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
DOI	10.1016/j.chroma.2017.05.007
Database	NAL-Catalogue (AGRICOLA)

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Article: Phytochelatins and monothiols in salt marsh plants and their relation with metal tolerance

Negrin, Vanesa L / Bárbara Teixeira / Carlos Vale / Rita M. Godinho / Rogério Mendes

Marine pollution bulletin. 2017 Aug. 15, v. 121, no. 1-2

2017

Abstract: Phytochelatins (PCs) and monothiols and their relation with trace element concentrations were studied in three plant species from two Portuguese salt marshes. Belowground tissues showed always higher element concentrations, while enhanced values of ... ...

Abstract	Phytochelatins (PCs) and monothiols and their relation with trace element concentrations were studied in three plant species from two Portuguese salt marshes. Belowground tissues showed always higher element concentrations, while enhanced values of monothiols were found in aboveground biomass. Glutathione was usually the most abundant monothiol. The concentration of total PCs was higher in leaves or stems than in roots of Halimione portulacoides and Sarcocornia perennis, while in Spartina maritima the highest concentrations were reported in large roots. PC2 was synthesized by all tissues and species and was higher in large roots of S. maritima. PC4 and PC5 were in high levels in small roots of S. maritima. PC2 was positively correlated with As, Zn and Pb. Although being the first evidence of PCs and monothiols in these species under natural conditions, our results do not point to a simple relationship with elements concentrations, suggesting a complex mechanism involved.
Keywords	aboveground biomass ; arsenic ; correlation ; glutathione ; lead ; leaves ; metal tolerance ; phytochelatins ; roots ; salt marsh plants ; salt marshes ; Sarcocornia ; Spartina ; stems ; tissues ; zinc
Language	English
Dates of publication	2017-0815
Size	p. 78-84.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	2001296-2
ISSN	1879-3363 ; 0025-326X
ISSN (online)	1879-3363
ISSN	0025-326X
DOI	10.1016/j.marpolbul.2017.05.045
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Article ; Online: Multitask ATPases (NBDs) of bacterial ABC importers type I and their interspecies exchangeability

Francisco Leisico / Lia M. Godinho / Inês C. Gonçalves / Sara P. Silva / Bruno Carneiro / Maria J. Romão / Teresa Santos-Silva / Isabel de Sá-Nogueira

Scientific Reports, Vol 10, Iss 1, Pp 1-

2020 Volume 17

Abstract: Abstract ATP-binding cassette (ABC) type I importers are widespread in bacteria and play a crucial role in its survival and pathogenesis. They share the same modular architecture comprising two intracellular nucleotide-binding domains (NBDs), two ... ...

Abstract	Abstract ATP-binding cassette (ABC) type I importers are widespread in bacteria and play a crucial role in its survival and pathogenesis. They share the same modular architecture comprising two intracellular nucleotide-binding domains (NBDs), two transmembrane domains (TMDs) and a substrate-binding protein. The NBDs bind and hydrolyze ATP, thereby generating conformational changes that are coupled to the TMDs and lead to substrate translocation. A group of multitask NBDs that are able to serve as the cellular motor for multiple sugar importers was recently discovered. To understand why some ABC importers share energy-coupling components, we used the MsmX ATPase from Bacillus subtilis as a model for biological and structural studies. Here we report the first examples of functional hybrid interspecies ABC type I importers in which the NBDs could be exchanged. Furthermore, the first crystal structure of an assigned multitask NBD provides a framework to understand the molecular basis of the broader specificity of interaction with the TMDs.
Keywords	Medicine ; R ; Science ; Q
Subject code	500
Language	English
Publishing date	2020-11-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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