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Article ; Online: Physiological responses of crossbred dairy cows in the transition period during summer and post-monsoon seasons

M. Sahana / K. S. Anil / Joseph Mathew / M. T. Dipu / Justin Davis / M. Shynu

Journal of Veterinary and Animal Sciences, Vol 54, Iss 3, Pp 791-

2023 Volume 798

Abstract: This study aims to measure the physiological responses of sixty clinically healthy periparturient crossbred multiparous dairy cows during the summer (February 2022–May 2022) and post-monsoon (October 2021–January 2022) seasons, with 30 animals in each ... ...

Abstract	This study aims to measure the physiological responses of sixty clinically healthy periparturient crossbred multiparous dairy cows during the summer (February 2022–May 2022) and post-monsoon (October 2021–January 2022) seasons, with 30 animals in each season. Physiological responses such as rectal temperature (°C), respiration rate (per minute), and pulse rate (per minute) of all animals were recorded weekly at 8.00 am and 3.00 pm during the transition period. Three weeks before parturition to three weeks after parturition was considered the transition period. The rectal temperature recorded at 3.00 pm in the summer season significantly (p <0.05) decreased on the day of calving and thereafter returned to pre-partum values, whereas the respiration rate significantly (p <0.05) elevated on the day of calving and thereafter showed no significant variation. Season-wise comparison of all physiological responses in the morning (8.00 am) and evening (3.00 pm) during the transition period showed significantly higher values in the summer season compared to the post-monsoon season. In both seasons evening (3.00 pm) physiological responses were significantly higher than the morning values (8.00 am).
Keywords	physiological parameters ; transition period ; summer ; post-monsoon ; Animal biochemistry ; QP501-801 ; Science (General) ; Q1-390
Subject code	630
Language	English
Publishing date	2023-09-01T00:00:00Z
Publisher	Director of Academics and Research, Kerala Veterinary and Animal Sciences University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Analysis of differential expression of microRNA, bta-miR-93 in lipopolysaccharide challenged peripheral blood mononuclear cells of cattle

A.B. Venu / P.D. Divya / R. Uma / V.P. Panicker / V. Ramnath / M. Shynu / M.V. Chinnu

Journal of Veterinary and Animal Sciences, Vol 54, Iss 2, Pp 457-

2023 Volume 464

Abstract: The present study was undertaken to analyse the differences in the microRNA (miRNA) expression in lipopolysaccharide (LPS) challenged and unchallenged peripheral blood mononuclear cells (PBMCs) of cattle. The research work was carried out in adult ... ...

Abstract	The present study was undertaken to analyse the differences in the microRNA (miRNA) expression in lipopolysaccharide (LPS) challenged and unchallenged peripheral blood mononuclear cells (PBMCs) of cattle. The research work was carried out in adult apparently healthy female crossbred cattle maintained at the University Livestock Farm and Fodder Research and Development Scheme, Mannuthy. The expression of miRNA and bta-miR-93 was profiled in the present study by qRT-PCR assay. In silico analysis was done using various online bioinformatic tools for the prediction of target genes, gene ontology analysis and also to study the cellular pathways associated to the target genes of bta-miR-93. The expression analysis of bta-miR- 93 showed decrease in its expression in LPS treated PBMCs when compared to control cells. Besides, significant enrichment of target genes of bta-miR-93 was noticed in many immune-related GO terms and in critical immune-associated cellular pathways. The findings of the current study will help in understanding the regulatory role of bta-miR-93 in LPS-mediated immune responses in cattle.
Keywords	microrna ; real-time pcr ; pbmcs ; lipopolysaccharide ; Animal biochemistry ; QP501-801 ; Science (General) ; Q1-390
Subject code	570
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	Director of Academics and Research, Kerala Veterinary and Animal Sciences University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Incomplete right bundle branch block associated with coagulation disturbance in mitral valvular disease in a dog – case report

Sindhu K. Rajan / J. Greeshma / Usha Narayana Pillai / S. Ajith Kumar / N. Madhavan Unny / G. Radhika / M. Shynu

Journal of Veterinary and Animal Sciences, Vol 54, Iss 3, Pp 859-

2023 Volume 862

Abstract: An incomplete right bundle branch block (RBBB) associated with coagulation disturbance in mitral valvular disease was diagnosed in a five-year-old female Dachshund. Morphologically moderate mitral valvular thickening and nodularity could be noticed on ... ...

Abstract	An incomplete right bundle branch block (RBBB) associated with coagulation disturbance in mitral valvular disease was diagnosed in a five-year-old female Dachshund. Morphologically moderate mitral valvular thickening and nodularity could be noticed on echocardiography. Prolonged activated partial thromboplastin time and thrombocytopaenia supported the probable underlying mechanism for myocardial infarction and RBBB. The animal showed clinical improvement after one month of therapy with enalapril and torasemide for mitral valve insufficiency.
Keywords	right bundle branch block ; mitral valve ; infarction ; Animal biochemistry ; QP501-801 ; Science (General) ; Q1-390
Language	English
Publishing date	2023-09-01T00:00:00Z
Publisher	Director of Academics and Research, Kerala Veterinary and Animal Sciences University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Post-thaw quality of Malabari buck semen with different freezing resilience

K. Lekshmi Bhai / B. Bibin Becha / C. Jayakumar: / Hiron, M. Harshan / M. Shynu / R. Thirupathy Venkatachalapathy

Journal of Veterinary and Animal Sciences, Vol 54, Iss 4, Pp 980-

2023 Volume 987

Abstract: The study was conducted to assess the difference in post-thaw quality parameters of Malabari buck spermatozoa of good and poor semen freezability. The ejaculates collected from Malabari bucks of good (n=3) and poor (n=3) semen freezability were pooled ... ...

Abstract	The study was conducted to assess the difference in post-thaw quality parameters of Malabari buck spermatozoa of good and poor semen freezability. The ejaculates collected from Malabari bucks of good (n=3) and poor (n=3) semen freezability were pooled separately as group A and group B, respectively after the initial evaluation. The extended semen in each group was processed and cryopreserved by the conventional method of static vapour freezing. After thawing, the quality of semen in both groups was assessed in terms of progressive motility, viability, morphological abnormalities, acrosome integrity, functional membrane integrity and lipid peroxidation status (MDA) of spermatozoa. Results showed that there was no significant difference between the fresh semen characteristics of group A and B bucks except for concentration, progressive motility and functional membrane integrity of spermatozoa. But, post-thaw quality of groups A and B differed significantly in terms of progressive motility (48.89 ± 0.64 % vs 18.06 ± 1.08 %), viability (55.17 ± 0.57 % vs 26.33 ± 0.95 %), acrosome integrity (62.44 ± 0.62 % vs 33.42 ± 0.34 %), functional membrane integrity (47.00 ± 0.36 % vs 23.36 ± 0.63 %) and MDA concentration (7.83 ± 0.29 nmol/mL vs 13.95 ± 0.08 nmol/mL) whereas morphological abnormalities (2.11 ± 0.08 % vs 2.39 ± 0.09 %) did not differ significantly. The findings of the present study showed that even with similar fresh semen characteristics, spermatozoa from different Malabari bucks have varying degrees of cryosurvivability.
Keywords	malabari buck spermatozoa ; good semen freezability ; poor semen freezability ; freezing resilience ; cryopreservation ; Animal biochemistry ; QP501-801 ; Science (General) ; Q1-390
Subject code	630
Language	English
Publishing date	2023-12-01T00:00:00Z
Publisher	Director of Academics and Research, Kerala Veterinary and Animal Sciences University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Occurrence of Campylobacter spp. in duck and associated environmental samples in Thrissur district#

T. R. Athulya / C. Latha / B. Sunil / J. Deepa / M. Shynu

Journal of Veterinary and Animal Sciences, Vol 52, Iss 4, Pp 325-

2021 Volume 330

Abstract: Campylobacteriosis caused by Campylobacter spp. is considered as the most common cause of bacterial diarrhoea in humans across the globe. The current research was undertaken to assess the occurrence of Campylobacter spp. in duck and the associated ... ...

Abstract	Campylobacteriosis caused by Campylobacter spp. is considered as the most common cause of bacterial diarrhoea in humans across the globe. The current research was undertaken to assess the occurrence of Campylobacter spp. in duck and the associated environmental samples. Among 220 samples analysed, 7.73 per cent samples revealed the presence of Campylobacter spp. Majority of the samples contained C. coli (4.55 per cent) and C. jejuni was detected in 3.18 per cent of the samples. The present study revealed a high occurrence of Campylobacter spp. in duck rearing facilities in Thrissur district, Kerala. As the demand for duck products is increasing every year, the risk of contamination by Campylobacter spp. has to be viewed seriously. The study revealed the importance of multifaceted one health approach including human medicine, veterinary medicine, epidemiology, environmental hygiene, public health institutes and epidemiological surveillance agencies to control food-borne diseases and up-gradation of biosecurity measures.
Keywords	campylobacter spp. one health ; Animal biochemistry ; QP501-801 ; Science (General) ; Q1-390
Subject code	630
Language	English
Publishing date	2021-10-01T00:00:00Z
Publisher	Director of Academics and Research, Kerala Veterinary and Animal Sciences University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Linkage disequilibrium over short physical genomic distances measured using medium density snp beadchip in native goat breeds of Kerala

Marykutty Thomas / G. Radhika / T.V. Aravindakshan / R. Thirupathy / K. Raji / M. Shynu

Journal of Veterinary and Animal Sciences, Vol 52, Iss 1, Pp 14-

2021 Volume 18

Abstract: The extent of linkage disequilibrium (LD) at genome wide level is crucial in determining the effectiveness of genomics tools in livestock breeding. The present population genomic study was conducted in native goat breeds of Kerala namely; Attapady Black ... ...

Abstract	The extent of linkage disequilibrium (LD) at genome wide level is crucial in determining the effectiveness of genomics tools in livestock breeding. The present population genomic study was conducted in native goat breeds of Kerala namely; Attapady Black goats (n=24) and Malabari goats (n=24) to characterise extent of LD within 40kbp marker interval using genome wide single nucleotide polymorphism (SNP) marker data obtained by SNP50 BeadChip genotyping. Extent of LD between bi allelic markers was measured using correlation coefficient (r2). Mean r2 between adjacent SNP pairs across all autosomes within 40Kbp marker interval was low (Attapady Black: 0.1336; Malabari: 0.1284). The LD varied across autosomes in native goats. It was the highest for SNP pairs on Capra hircus autosome 6 (CHI 6) and the lowest for SNP pairs harboured in CHI 28 in Attapady Black goats and for SNP pairs in CHI 29 in Malabari goats. The low LD estimates indicate the genetically diverse nature of native goats. Current results also imply that denser SNP beadchip array with inter marker interval of below 40kbp would be desirable for effective genome wide association study (GWAS) and genomic selection in native goats.
Keywords	linkage disequilibrium ; goat ; snp beadchip ; Animal biochemistry ; QP501-801 ; Science (General) ; Q1-390
Subject code	630
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Director of Academics and Research, Kerala Veterinary and Animal Sciences University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: HIgher concentration of haptoglogin indicates transient inflammation and negative energy balance in transition cows

S.V. Vasudhar Bhat / J.P. Anisha / M. Shynu / K.K. Jayavardhanan / V. Ramnath

Journal of Veterinary and Animal Sciences, Vol 51, Iss 1, Pp 31-

2020 Volume 33

Abstract: Haptoglobin is an acute phase protein that is of diagnostic significance in dairy cattle. In the present study, haptoglobin level was assessed during the periparturient period in 15 clinically healthy pregnant cows. The mean concentration of haptoglobin ... ...

Abstract	Haptoglobin is an acute phase protein that is of diagnostic significance in dairy cattle. In the present study, haptoglobin level was assessed during the periparturient period in 15 clinically healthy pregnant cows. The mean concentration of haptoglobin during transition was found to be 5.60 ± 0.54 mg/dL, whereas the concentration during the period immediately before and after transition was 4.80 ± 0.59 mg/ dL. The concentration obtained was greater than the reported concentration of 2mg/dL or less, in healthy ruminants. The higher concentrations observed were also associated with a higher concentration of serum non-esterified fatty acids of 0.576 ± 0.10 mmoles/L during transition and 0.328 ± 0.03 mmoles/L immediately outside the transition period. Increased haptoglobin might indicate the transient inflammation and a response to negative energy balance during transition in dairy cows.
Keywords	haptaglobulin ; non esterified fatty acid ; transition ; Animal biochemistry ; QP501-801 ; Science (General) ; Q1-390
Subject code	630
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Director of Academics and Research, Kerala Veterinary and Animal Sciences University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Qualitative and quantitative analysis (GC-MS) of methanol extract of Crataeva nurvala stem bark

K. K. Aathira / Bibu John Kariyil / G. Dhanusha / J. S. Haima / S. Sujith / M. Shynu / A. R. Nisha

Journal of Veterinary and Animal Sciences, Vol 52, Iss 2, Pp 135-

2021 Volume 141

Abstract: Medicinal plants are precious source of bioactive compounds which possess a range of beneficial properties and serve as the major source of medicine for a large proportion of population across the world. Since ancient times, Crataeva nurvala has been ... ...

Abstract	Medicinal plants are precious source of bioactive compounds which possess a range of beneficial properties and serve as the major source of medicine for a large proportion of population across the world. Since ancient times, Crataeva nurvala has been used as a vital herb in Ayurvedic system of medicine. In Unani system of medicine the bark of C. nurvala is used as an appetite stimulant and as an agent to decrease the secretion of bile and phlegm. In the present study, the methanol extract of stem bark of C. nurvala was analysed for preliminary phytochemicals and the chemical profiling of the extract was illustrated using gas chromatography mass spectrometry (GC-MS) analysis. The phytochemical analysis revealed that the plant extract contained alkaloids, steroids and triterpenoids. The GC-MS analysis determined the presence of different compounds of biological importance. The identification and characterisation of the phytoconstituents in the extract could pave the way for the discovery of new drugs for various ailments.
Keywords	crataeva nurvala ; triterpenes ; alkaloids ; steroids ; Animal biochemistry ; QP501-801 ; Science (General) ; Q1-390
Subject code	540
Language	English
Publishing date	2021-06-01T00:00:00Z
Publisher	Director of Academics and Research, Kerala Veterinary and Animal Sciences University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Role of microRNA, bta-miR-375 in Immune Sturdiness of Vechur: The Native Cattle Breed of Kerala, India.

P D, Divya / M, Shynu / K K, Jayavardhanan / R, Uma / T V, Aravindakshan / G, Radhika / V B, Sameer Kumar / Asaf, Muhasin / Sebastian, Renjith

Heliyon

2023 Volume 9, Issue 12, Page(s) e22683

Abstract: In the present study, next generation sequencing was employed to identify and explore the differential expression profiles of microRNAs (miRNAs) in peripheral blood mononuclear cells (PBMCs) of crossbred ( ...

Abstract	In the present study, next generation sequencing was employed to identify and explore the differential expression profiles of microRNAs (miRNAs) in peripheral blood mononuclear cells (PBMCs) of crossbred (
Language	English
Publishing date	2023-11-22
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e22683
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: HAEMATOLOGICAL OBSERVATIONS IN TWENTY CAPTIVE MALE SAMBAR DEER (Rusa unicolor)

Joju Johns / George Chandy / Jacob Alexander / S. Sooryadas / K.D. John Martin / M. Shynu / S. Ajithkumar

Journal of Veterinary and Animal Sciences, Vol 49, Iss 1, Pp 22-

2018 Volume 24

Abstract: Haematological study was conducted in twenty apparently healthy adult male Sambar Deer maintained at Zoological Gardens, Thiruvananthapuram, which underwent vasectomy under general anaesthesia. Samples were analysed usingan automatic haematology analyser ...

Abstract	Haematological study was conducted in twenty apparently healthy adult male Sambar Deer maintained at Zoological Gardens, Thiruvananthapuram, which underwent vasectomy under general anaesthesia. Samples were analysed usingan automatic haematology analyser calibratedforcaprine blood. The haemoglobin concentration, volume of packed red cells and total erythrocyte count were 9.6±0.3 g/dL, 29.3±0.7 % and 5.8±0.2×106 /µL respectively. The total leucocyte count was 3.6±0.2×103/µL and granulocyte, lymphocyte and monocyte counts were 47±1 %, 47±1 % and 6±1 % respectively
Keywords	sambar deer ; rusa unicolor ; haematology ; blood indices ; Animal biochemistry ; QP501-801 ; Science (General) ; Q1-390
Language	English
Publishing date	2018-01-01T00:00:00Z
Publisher	Director of Academics and Research, Kerala Veterinary and Animal Sciences University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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