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Article ; Online: Antileishmanial Effects of Acetylene Acetogenins from Seeds of Porcelia macrocarpa (Warm.) R.E. Fries (Annonaceae) and Semisynthetic Derivatives

Ivanildo A. Brito / Fernanda Thevenard / Thais A. Costa-Silva / Samuel S. Oliveira / Rodrigo L. O. R. Cunha / Emerson A. de Oliveira / Patricia Sartorelli / Rafael C. Guadagnin / Maiara M. Romanelli / Andre G. Tempone / João Henrique G. Lago

Molecules, Vol 27, Iss 893, p

2022 Volume 893

Abstract: As part of our continuous studies involving the prospection of natural products from Brazilian flora aiming at the discovery of prototypes for the development of new antiparasitic drugs, the present study describes the isolation of two natural acetylene ... ...

Abstract	As part of our continuous studies involving the prospection of natural products from Brazilian flora aiming at the discovery of prototypes for the development of new antiparasitic drugs, the present study describes the isolation of two natural acetylene acetogenins, ( 2S , 3R , 4R )-3-hydroxy-4-methyl-2-( n -eicos-11′-yn-19′-enyl)butanolide ( 1 ) and ( 2S , 3R , 4R )-3-hydroxy-4-methyl-2-( n -eicos-11′-ynyl)butanolide ( 2 ), from the seeds of Porcelia macrocarpa (Warm.) R.E. Fries (Annonaceae). Using an ex-vivo assay, compound 1 showed an IC 50 value of 29.9 μM against the intracellular amastigote forms of Leishmania (L.) infantum , whereas compound 2 was inactive. These results suggested that the terminal double bond plays an important role in the activity. This effect was also observed for the semisynthetic acetylated ( 1a and 2a ) and eliminated ( 1b and 2b ) derivatives, since only compounds containing a double bond at C-19 displayed activity, resulting in IC 50 values of 43.3 μM ( 1a ) and 23.1 μM ( 1b ). In order to evaluate the effect of the triple bond in the antileishmanial potential, the mixture of compounds 1 + 2 was subjected to catalytic hydrogenation to afford a compound 3 containing a saturated side chain. The antiparasitic assays performed with compound 3 , acetylated ( 3a ), and eliminated ( 3b ) derivatives confirmed the lack of activity. Furthermore, an in-silico study using the SwissADME online platform was performed to bioactive compounds 1 , 1a , and 1b in order to investigate their physicochemical parameters, pharmacokinetics, and drug-likeness. Despite the reduced effect against amastigote forms of the parasite to the purified compounds, different mixtures of compounds 1 + 2 , 1a + 2a , and 1b + 2b were prepared and exhibited IC 50 values ranging from 7.9 to 38.4 μM, with no toxicity for NCTC mammalian cells (CC 50 > 200 μM). Selectivity indexes to these mixtures ranged from >5.2 to >25.3. The obtained results indicate that seeds of Porcelia macrocarpa are a promising source of ...
Keywords	Porcelia macrocarpa ; acetylene acetogenins ; Leishmania (L.) infantum ; leishmaniasis ; Organic chemistry ; QD241-441
Subject code	540
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Wntless regulates lipogenic gene expression in adipocytes and protects against diet-induced metabolic dysfunction

Devika P. Bagchi / Ziru Li / Callie A. Corsa / Julie Hardij / Hiroyuki Mori / Brian S. Learman / Kenneth T. Lewis / Rebecca L. Schill / Steven M. Romanelli / Ormond A. MacDougald

Molecular Metabolism, Vol 39, Iss , Pp - (2020)

2020

Abstract: Objective: Obesity is a key risk factor for many secondary chronic illnesses, including type 2 diabetes and cardiovascular disease. Canonical Wnt/β-catenin signaling is established as an important endogenous inhibitor of adipogenesis. This pathway is ... ...

Abstract	Objective: Obesity is a key risk factor for many secondary chronic illnesses, including type 2 diabetes and cardiovascular disease. Canonical Wnt/β-catenin signaling is established as an important endogenous inhibitor of adipogenesis. This pathway is operative in mature adipocytes; however, its roles in this context remain unclear due to complexities of Wnt signaling and differences in experimental models. In this study, we used novel cultured cell and mouse models to investigate functional roles of Wnts secreted from adipocytes. Methods: We generated adipocyte-specific Wntless (Wls) knockout mice and cultured cell models to investigate molecular and metabolic consequences of disrupting Wnt secretion from mature adipocytes. To characterize Wls-deficient cultured adipocytes, we evaluated the expression of Wnt target and lipogenic genes and the downstream functional effects on carbohydrate and lipid metabolism. We also investigated the impact of adipocyte-specific Wls deletion on adipose tissues and global glucose metabolism in mice fed normal chow or high-fat diets. Results: Many aspects of the Wnt signaling apparatus are expressed and operative in mature adipocytes, including the Wnt chaperone Wntless. Deletion of Wntless in cultured adipocytes results in the inhibition of de novo lipogenesis and lipid monounsaturation, likely through repression of Srebf1 (SREBP1c) and Mlxipl (ChREBP) and impaired cleavage of immature SREBP1c into its active form. Adipocyte-specific Wls knockout mice (Wls-/-) have lipogenic gene expression in adipose tissues and isolated adipocytes similar to that of controls when fed a normal chow diet. However, closer investigation reveals that a subset of Wnts and downstream signaling targets are upregulated within stromal-vascular cells of Wls-/- mice, suggesting that adipose tissues defend loss of Wnt secretion from adipocytes. Interestingly, this compensation is lost with long-term high-fat diet challenges. Thus, after six months of a high-fat diet, Wls-/- mice are characterized by ...
Keywords	Wntless ; Wnt signaling ; Adipose tissue ; Lipogenesis ; Adipocyte ; Metabolism ; Internal medicine ; RC31-1245
Subject code	570
Language	English
Publishing date	2020-09-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: The INSIEME seismic network

T. A. Stabile / V. Serlenga / C. Satriano / M. Romanelli / E. Gueguen / M. R. Gallipoli / E. Ripepi / J.-M. Saurel / S. Panebianco / J. Bellanova / E. Priolo

Earth System Science Data, Vol 12, Pp 519-

a research infrastructure for studying induced seismicity in the High Agri Valley (southern Italy)

2020 Volume 538

Abstract: The High Agri Valley is a tectonically active area in southern Italy characterized by high seismic hazard related to fault systems capable of generating up to M =7 earthquakes (i.e. the 1857 M w =7 Basilicata earthquake). In addition to the natural ... ...

Abstract	The High Agri Valley is a tectonically active area in southern Italy characterized by high seismic hazard related to fault systems capable of generating up to M =7 earthquakes (i.e. the 1857 M w =7 Basilicata earthquake). In addition to the natural seismicity, two different clusters of induced microseismicity were recognized to be caused by industrial operations carried out in the area: (1) the water loading and unloading operations in the Pertusillo artificial reservoir and (2) the wastewater disposal at the Costa Molina 2 injection well. The twofold nature of the recorded seismicity in the High Agri Valley makes it an ideal study area to deepen the understanding of driving processes of both natural and anthropogenic earthquakes and to improve the current methodologies for the discrimination between natural and induced seismic events by collecting high-quality seismic data. Here we present the dataset gathered by the INSIEME seismic network that was installed in the High Agri Valley within the SIR-MIUR research project INSIEME (INduced Seismicity in Italy: Estimation, Monitoring, and sEismic risk mitigation). The seismic network was planned with the aim to study the two induced seismicity clusters and to collect a full range of open-access data to be shared with the whole scientific community. The seismic network is composed of eight stations deployed in an area of 17 km×11 km around the two clusters of induced microearthquakes, and it is equipped with triaxial weak-motion broadband sensors placed at different depths down to 50 m. It allows us to detect induced microearthquakes, local and regional earthquakes, and teleseismic events from continuous data streams transmitted in real time to the CNR-IMAA Data Centre. The network has been registered at the International Federation of Digital Seismograph Networks (FDSN) with code 3F. Data collected until the end of the INSIEME project (23 March 2019) are already released with open-access policy through the FDSN web services and are available from IRIS DMC ( ...
Keywords	Environmental sciences ; GE1-350 ; Geology ; QE1-996.5
Subject code	550
Language	English
Publishing date	2020-03-01T00:00:00Z
Publisher	Copernicus Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: OGS improvements in 2012 in running the North-eastern Italy Seismic Network

D. Pesaresi / M. Romanelli / C. Barnaba / P. L. Bragato / G. Durì

Advances in Geosciences, Vol 36, Pp 61-

the Ferrara VBB borehole seismic station

2014 Volume 67

Abstract: The Centro di Ricerche Sismologiche (CRS, Seismological Research Centre) of the Istituto Nazionale di Oceanografia e di Geofisica Sperimentale (OGS, Italian National Institute for Oceanography and Experimental Geophysics) in Udine (Italy) after the ... ...

Abstract	The Centro di Ricerche Sismologiche (CRS, Seismological Research Centre) of the Istituto Nazionale di Oceanografia e di Geofisica Sperimentale (OGS, Italian National Institute for Oceanography and Experimental Geophysics) in Udine (Italy) after the strong earthquake of magnitude M =6.4 occurred in 1976 in the Italian Friuli-Venezia Giulia region, started to operate the North-eastern Italy Seismic Network: it currently consists of 17 very sensitive broad band and 18 simpler short period seismic stations, all telemetered to and acquired in real time at the OGS-CRS data centre in Udine. Real time data exchange agreements in place with other Italian, Slovenian, Austrian and Swiss seismological institutes lead to a total number of about 100 seismic stations acquired in real time, which makes the OGS the reference institute for seismic monitoring of North-eastern Italy. The south-western edge of the OGS seismic network (Fig. 1) stands on the Po alluvial basin: earthquake localization and characterization in this area is affected by the presence of soft alluvial deposits. OGS ha already experience in running a local seismic network in high noise conditions making use of borehole installations in the case of the micro-seismicity monitoring of a local gas storage site for a private company. Following the ML = 5.9 earthquake that struck the Emilia region around Ferrara in Northern Italy on 20 May 2012 at 02:03:53 UTC, a cooperation of Istituto Nazionale di Geofisica e Vulcanologia, OGS, the Comune di Ferrara and the University of Ferrara lead to the reinstallation of a previously existing very broad band (VBB) borehole seismic station in Ferrara. The aim of the OGS intervention was on one hand to extend its real time seismic monitoring capabilities toward South-West, including Ferrara and its surroundings, and on the other hand to evaluate the seismic response at the site. We will describe improvements in running the North-eastern Italy Seismic Network, including details of the Ferrara VBB borehole station configuration ...
Keywords	Science ; Q ; Geology ; QE1-996.5 ; Dynamic and structural geology ; QE500-639.5
Subject code	500
Language	English
Publishing date	2014-07-01T00:00:00Z
Publisher	Copernicus Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: The molecular and metabolic program by which white adipocytes adapt to cool physiologic temperatures.

Hiroyuki Mori / Colleen E Dugan / Akira Nishii / Ameena Benchamana / Ziru Li / Thomas S Cadenhead / Arun K Das / Charles R Evans / Katherine A Overmyer / Steven M Romanelli / Sydney K Peterson / Devika P Bagchi / Callie A Corsa / Julie Hardij / Brian S Learman / Mahmoud El Azzouny / Joshua J Coon / Ken Inoki / Ormond A MacDougald

PLoS Biology, Vol 19, Iss 5, p e

2021 Volume 3000988

Abstract: Although visceral adipocytes located within the body's central core are maintained at approximately 37°C, adipocytes within bone marrow, subcutaneous, and dermal depots are found primarily within the peripheral shell and generally exist at cooler ... ...

Abstract	Although visceral adipocytes located within the body's central core are maintained at approximately 37°C, adipocytes within bone marrow, subcutaneous, and dermal depots are found primarily within the peripheral shell and generally exist at cooler temperatures. Responses of brown and beige/brite adipocytes to cold stress are well studied; however, comparatively little is known about mechanisms by which white adipocytes adapt to temperatures below 37°C. Here, we report that adaptation of cultured adipocytes to 31°C, the temperature at which distal marrow adipose tissues and subcutaneous adipose tissues often reside, increases anabolic and catabolic lipid metabolism, and elevates oxygen consumption. Cool adipocytes rely less on glucose and more on pyruvate, glutamine, and, especially, fatty acids as energy sources. Exposure of cultured adipocytes and gluteal white adipose tissue (WAT) to cool temperatures activates a shared program of gene expression. Cool temperatures induce stearoyl-CoA desaturase-1 (SCD1) expression and monounsaturated lipid levels in cultured adipocytes and distal bone marrow adipose tissues (BMATs), and SCD1 activity is required for acquisition of maximal oxygen consumption at 31°C.
Keywords	Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2021-05-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Comparison of Engineered Peptide-Glycosaminoglycan Microfibrous Hybrid Scaffolds for Potential Applications in Cartilage Tissue Regeneration

Steven M. Romanelli / Grant A. Knoll / Anthony M. Santora / Alexandra M. Brown / Ipsita A. Banerjee

Fibers, Vol 3, Iss 3, Pp 265-

2015 Volume 295

Abstract: Advances in tissue engineering have enabled the ability to design and fabricate biomaterials at the nanoscale that can actively mimic the natural cellular environment of host tissue. Of all tissues, cartilage remains difficult to regenerate due to its ... ...

Abstract	Advances in tissue engineering have enabled the ability to design and fabricate biomaterials at the nanoscale that can actively mimic the natural cellular environment of host tissue. Of all tissues, cartilage remains difficult to regenerate due to its avascular nature. Herein we have developed two new hybrid polypeptide-glycosaminoglycan microfibrous scaffold constructs and compared their abilities to stimulate cell adhesion, proliferation, sulfated proteoglycan synthesis and soluble collagen synthesis when seeded with chondrocytes. Both constructs were designed utilizing self-assembled Fmoc-protected valyl cetylamide nanofibrous templates. The peptide components of the constructs were varied. For Construct I a short segment of dentin sialophosphoprotein followed by Type I collagen were attached to the templates using the layer-by-layer approach. For Construct II, a short peptide segment derived from the integrin subunit of Type II collagen binding protein expressed by chondrocytes was attached to the templates followed by Type II collagen. To both constructs, we then attached the natural polymer N-acetyl glucosamine, chitosan. Subsequently, the glycosaminoglycan chondroitin sulfate was then attached as the final layer. The scaffolds were characterized by Fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), atomic force microscopy and scanning electron microscopy. In vitro culture studies were carried out in the presence of chondrocyte cells for both scaffolds and growth morphology was determined through optical microscopy and scanning electron microscopy taken at different magnifications at various days of culture. Cell proliferation studies indicated that while both constructs were biocompatible and supported the growth and adhesion of chondrocytes, Construct II stimulated cell adhesion at higher rates and resulted in the formation of three dimensional cell-scaffold matrices within 24 h. Proteoglycan synthesis, a hallmark of chondrocyte cell differentiation, was also higher ...
Keywords	cartilage ; scaffolds ; collagen ; chitosan ; fibers ; Chemicals: Manufacture ; use ; etc ; TP200-248 ; Textile bleaching ; dyeing ; printing ; TP890-933 ; Biology (General) ; QH301-705.5 ; Physics ; QC1-999
Subject code	540 ; 571
Language	English
Publishing date	2015-07-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Temporary seismic monitoring of the Sulmona area (Abruzzo, Italy)

M. A. Romano / R. de Nardis / M. Garbin / L. Peruzza / E. Priolo / G. Lavecchia / M. Romanelli

Natural Hazards and Earth System Sciences, Vol 13, Iss 11, Pp 2727-

a quality study of microearthquake locations

2013 Volume 2744

Abstract: Thanks to the installation of a temporary seismic network, a microseismicity study has been conducted in the Sulmona area (Abruzzo, Italy) with the aim of increasing the knowledge of seismogenic potential of existing active faults. In this work the first ...

Abstract	Thanks to the installation of a temporary seismic network, a microseismicity study has been conducted in the Sulmona area (Abruzzo, Italy) with the aim of increasing the knowledge of seismogenic potential of existing active faults. In this work the first 7 months (from 27 May to 31 December 2009) of recorded data have been analysed over a total period of acquisition of about 30 months. Using a semi-automatic procedure, more than 800 local earthquakes have been detected, which highlights the previously unknown background seismicity. About 70% of these events have been relocated using a 1-D velocity model estimated specifically for the Sulmona area. The integration of temporary network data with all the other data available in the region enables us to obtain a statistically more robust data set of earthquake locations. Both the final hypocentral solutions and phase pickings are released as a supplement; an appendix also describes phase readings' quality with respect to weighting schemes used by location algorithms. Local magnitude values of the newly detected events range between −1.5 and 3.7 and the completeness magnitude for the Sulmona area during the study period is about 1.1. Duration magnitude coefficients have been estimated as well for comparison/integration purposes. The local Gutenberg–Richter relationship, estimated from the microseismic data, features a low b value, tentatively suggesting that the Sulmona area may be currently undergoing high-stress conditions, in agreement with other recent studies. The time–space distribution of the seismic activity with respect to the known active faults as well the seismogenic layer thickness are preliminarily investigated.
Keywords	Environmental technology. Sanitary engineering ; TD1-1066 ; Geography. Anthropology. Recreation ; G ; Environmental sciences ; GE1-350 ; Geology ; QE1-996.5
Language	English
Publishing date	2013-11-01T00:00:00Z
Publisher	Copernicus Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Temperature and pH sensors based on graphenic materials

Salvo, P / A. Caneschi / A. Paolicchi / B. Cortigiani / B. Melai / C. Paoletti / F. Di Francesco / G. Lorenzetti / I. Scataglini / M. Mannini / M. Romanelli / N. Calisi / R. Fuoco / T. Lomonaco / V. Dini

Biosensors & bioelectronics. 2017 May 15, v. 91

2017

Abstract: Point-of-care applications and patients’ real-time monitoring outside a clinical setting would require disposable and durable sensors to provide better therapies and quality of life for patients. This paper describes the fabrication and performances of a ...

Abstract	Point-of-care applications and patients’ real-time monitoring outside a clinical setting would require disposable and durable sensors to provide better therapies and quality of life for patients. This paper describes the fabrication and performances of a temperature and a pH sensor on a biocompatible and wearable board for healthcare applications. The temperature sensor was based on a reduced graphene oxide (rGO) layer that changed its electrical resistivity with the temperature. When tested in a human serum sample between 25 and 43°C, the sensor had a sensitivity of 110±10Ω/°C and an error of 0.4±0.1°C compared with the reference value set in a thermostatic bath. The pH sensor, based on a graphene oxide (GO) sensitive layer, had a sensitivity of 40±4mV/pH in the pH range between 4 and 10. Five sensor prototypes were tested in a human serum sample over one week and the maximum deviation of the average response from reference values obtained by a glass electrode was 0.2pH units. For biological applications, the temperature and pH sensors were successfully tested for in vitro cytotoxicity with human fibroblast cells (MRC-5) over 24h.
Keywords	biosensors ; blood serum ; cytotoxicity ; electrical resistance ; electrodes ; fibroblasts ; glass ; graphene oxide ; health services ; humans ; in vitro studies ; monitoring ; normal values ; patients ; pH ; prototypes ; quality of life ; temperature
Language	English
Dates of publication	2017-0515
Size	p. 870-877.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1011023-9
ISSN	1873-4235 ; 0956-5663
ISSN (online)	1873-4235
ISSN	0956-5663
DOI	10.1016/j.bios.2017.01.062
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: EXPERIMENTAL STUDY OF 3D SELF-ASSEMBLED PHOTONIC CRYSTALS AND COLLOIDAL CORE-SHELL SEMICONDUCTOR QUANTUM DOTS

Pham Thu Nga / Vu Duc Chinh / Nguyen Xuan Nghia / Nguyen Viet Huy / Dao Nguyen Thuan / Pham Thai Cuong / Chu Viet Ha / Do Thuy Chi / Nguyen Nhu Dat / Le A Lan Anh / C. Barthou / P. Benalloul / M. Romanelli / A. Maître

ASEAN Journal on Science and Technology for Development, Vol 24, Iss 1&2, Pp 161-

2017 Volume 170

Abstract: In this contribution we present an experimental study of 3D opal photonic crystals. The samples are opals constituted by colloidal silica spheres, realized with self-assembly technique. The sphere diameter is selected in order to obtain coupling of the ... ...

Abstract	In this contribution we present an experimental study of 3D opal photonic crystals. The samples are opals constituted by colloidal silica spheres, realized with self-assembly technique. The sphere diameter is selected in order to obtain coupling of the photonic band gap with the emission from CdSe/ZnS colloidal quantum dots. The quantum dots infiltrated in the opals is expected to be enhanced or suppressed depending on the detection angle from the photonic crystal. The structural and optical characterization of the SiO2 opal photonic crystals are performed by field-emission scanning electron microscopy and reflectivity spectroscopy. Measurements performed on samples permits to put into evidence the influence of the different preparation methods on the optical properties. Study of self-activated luminescence of the pure opals is also presented. It is shown that the luminescence of the sample with QDs have original QD emission and not due to the photonic crystal structure. The optical properties of colloidal core-shell semiconductor quantum dots of CdSe/ZnS which are prepared in our lab will be mention.
Keywords	Photonic crystals ; SiO2 ; opals ; CdSe/ZnS quantum dots ; optical properties ; Technology (General) ; T1-995 ; Science (General) ; Q1-390
Subject code	535 ; 530
Language	English
Publishing date	2017-11-01T00:00:00Z
Publisher	Universitas Gadjah Mada
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Identification of bivalve species at an early developmental stage through PCR-SSCP and sequence analysis of partial 18S rDNA

Livi, S / C. Cordisco / C. Damiani / D. Crosetti / M. Romanelli

Marine biology. 2006 Aug., v. 149, no. 5

2006

Abstract: The notorious difficulties encountered in species identification of bivalve larvae through morphological characters have given rise to several alternative molecular approaches. In the present work, we propose a method based on PCR-SSCP combined with ... ...

Abstract	The notorious difficulties encountered in species identification of bivalve larvae through morphological characters have given rise to several alternative molecular approaches. In the present work, we propose a method based on PCR-SSCP combined with sequencing of partial 18S rDNA region, in which a large number of larvae can be scored and species identified with minimum sequencing effort. A primer set was developed to amplify a taxonomically informative 18S region of fragment size suitable for high resolution PCR-SSCP. The method developed is fast processing and has the potential of identifying most species present in a plankton sample with low economic efforts. Those species for which partial 18S sequences are not yet available from GenBank, can be identified in families or at higher categories. The method was also tested on nine species and two subspecies of commercial importance in Italy to be carried out by the use of PCR-SSCP alone without sequencing.
Keywords	larvae ; oligodeoxyribonucleotides ; plankton ; polymerase chain reaction ; ribosomal DNA ; sequence analysis ; single-stranded conformational polymorphism ; species identification ; Italy
Language	English
Dates of publication	2006-08
Size	p. 1149-1161.
Publishing place	Springer-Verlag
Document type	Article
ZDB-ID	1117-4
ISSN	1432-1793 ; 0025-3162
ISSN (online)	1432-1793
ISSN	0025-3162
DOI	10.1007/s00227-006-0296-2
Database	NAL-Catalogue (AGRICOLA)

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