Article ; Online: Combined RNAscope and immunohistochemistry staining on duodenal paraffin sections as a new tool to reveal cytolytic potential of intraepithelial lymphocytes
Journal of Immunological Methods. 2023 June, v. 517 p.113470-
2023
Abstract: Immunohistochemistry (IHC) is a consolidated technique for the identification of surface and cytoplasmic antigens in cells or tissue sections using specific antibodies, yet simultaneous detection of two markers on the same cell may be difficult to ... ...
Abstract | Immunohistochemistry (IHC) is a consolidated technique for the identification of surface and cytoplasmic antigens in cells or tissue sections using specific antibodies, yet simultaneous detection of two markers on the same cell may be difficult to achieve. Here we develop a protocol to perform a double staining using RNAscope, a new in-situ hybridization (ISH) technology, to visualize perforin transcripts, and classical IHC to visualize either CD8 or TcRγδ positive intraepithelial lymphocytes (IELs) in small intestinal paraffin sections of celiac disease (CD) patients. This double assay will allow to investigate the cytotoxic properties of two subsets of IELs in different stages of CD, thus contributing to understand the events leading to tissue destruction and healing. |
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Keywords | celiac disease ; cytotoxicity ; immunohistochemistry ; in situ hybridization ; intestines ; RNAscope ; Intraepithelial lymphocytes ; Perforin ; AMP ; AP ; CD ; CD8 ; FFPE ; HRP ; HybEZ oven ; IELs ; IHC ; ISH ; NKRs ; TcR |
Language | English |
Dates of publication | 2023-06 |
Publishing place | Elsevier B.V. |
Document type | Article ; Online |
Note | Pre-press version ; Use and reproduction |
ZDB-ID | 120142-6 |
ISSN | 1872-7905 ; 0022-1759 |
ISSN (online) | 1872-7905 |
ISSN | 0022-1759 |
DOI | 10.1016/j.jim.2023.113470 |
Database | NAL-Catalogue (AGRICOLA) |
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