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  1. Article: Adrenal atrophy and thyroid inhibition following PABA.

    MCCARTHY, J L / MURPHREE, R L

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)

    2003  Volume 105, Page(s) 515–517

    MeSH term(s) 4-Aminobenzoic Acid/pharmacology ; Antithyroid Agents/pharmacology ; Atrophy ; Humans ; Thyroid Gland
    Chemical Substances Antithyroid Agents ; 4-Aminobenzoic Acid (TL2TJE8QTX)
    Language English
    Publishing date 2003-09-17
    Publishing country United States
    Document type Journal Article
    ZDB-ID 4015-0
    ISSN 1535-3699 ; 1525-1373 ; 0037-9727
    ISSN (online) 1535-3699 ; 1525-1373
    ISSN 0037-9727
    DOI 10.3181/00379727-105-26161
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ua VI Zs.111: Show issues Location:
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  2. Article: Adrenocorticotropin administration during early gestation on conceptus development in swine.

    Arnold, J D / Kattesh, H G / Chen, T T / Murphree, R L

    Theriogenology

    2006  Volume 17, Issue 5, Page(s) 475–484

    Abstract: The purpose of this study was to examine the effect of exogenous adrenocorticotropin (ACTH), administered to gilts during early stages of gestation, upon fetal survival and various maternal and conceptus parameters. Forty-eight gilts of approximately 6-7 ...

    Abstract The purpose of this study was to examine the effect of exogenous adrenocorticotropin (ACTH), administered to gilts during early stages of gestation, upon fetal survival and various maternal and conceptus parameters. Forty-eight gilts of approximately 6-7 months of age were bred by means of artificial insemination after detection of the second estrus and randomly allotted to one of 12 treatment-period groups. Treatment consisted of a daily intramuscular injection of 0, 40 or 80 U.S.P. units of a long acting ACTH preparation for a period of five days. The injection periods were 1-5, 6-10, 11-15 or 16-20 days of gestation with day one corresponding to 48 hours post-estrus detection. All gilts were slaughtered at approximately 37 days of gestation. Forty-two of the 48 inseminated gilts conceived. Conception rate was not different (P>.10) among the 12 treatment-period combinations. Percent fetal survival was greater (P<.09) in gilts receiving 80 U.S.P. units of ACTH (82 +/- 4.3%; X +/- SEM ) than in gilts receiving 40 U.S.P. units of ACTH (68.8 +/- 4.5%). The percent fetal survival in the control group (71.7 +/- 3.9%) was not different (P>.10) from either of the two ACTH treatment groups. A significant (P<.05) treatment by period interaction for percent fetal survival was observed. The lowest percent fetal survival (48.0 +/- 9.0%) was observed in gilts receiving 40 U.S.P. units of ACTH on day 11-15 of gestation. No significant (P>.10) differences were detected among the 12 treatment-period combinations for any of the maternal or conceptus parameters measured.
    Language English
    Publishing date 2006-04-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 189232-0
    ISSN 1879-3231 ; 0093-691X
    ISSN (online) 1879-3231
    ISSN 0093-691X
    DOI 10.1016/0093-691x(82)90174-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3929: Show issues Location:
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  3. Article: Uptake and retention of Vibrio cholerae O1 in the Eastern oyster, Crassostrea virginica.

    Murphree, R L / Tamplin, M L

    Applied and environmental microbiology

    1995  Volume 61, Issue 10, Page(s) 3656–3660

    Abstract: Vibrio cholerae 01, the causative agent of cholera, is known to persist in estuarine environments as endogenous microflora. The recent introduction of V. cholerae 01 into estuaries of the North and South American continents has stimulated the need to ... ...

    Abstract Vibrio cholerae 01, the causative agent of cholera, is known to persist in estuarine environments as endogenous microflora. The recent introduction of V. cholerae 01 into estuaries of the North and South American continents has stimulated the need to determine the effect of controlled purification on reducing this pathogen in edible molluscan shellfish. Experiments defined parameters for the uptake and retention of V. cholerae 01 in tissues of Crassostrea virginica, and these parameters were compared with those for Escherichia coli and Salmonella tallahassee, bacteria which are usually eliminated from moderately contaminated shellfish within 48 h. Oysters accumulated greater concentrations of V. cholerae 01 than E. coli and S. tallahassee. When V. cholerae 01 was exposed to controlled purification at 15, 19 and 25 degrees C over 48 h, it persisted in oysters at markedly higher levels than E. coli and S. tallahassee. The concentration of a V. cholerae 01-specific agglutinin did not positively correlate with the uptake or retention of V. cholerae 01. These data show that state and federally approved controlled purification techniques are not effective at reducing V. cholerae 01 in oysters.
    MeSH term(s) Agglutinins/analysis ; Animals ; Cholera/etiology ; Cholera/prevention & control ; Cholera/transmission ; Escherichia coli/isolation & purification ; Escherichia coli/radiation effects ; Foodborne Diseases/etiology ; Foodborne Diseases/prevention & control ; Humans ; Ostreidae/microbiology ; Salmonella/isolation & purification ; Salmonella/radiation effects ; Shellfish/microbiology ; Temperature ; Ultraviolet Rays ; Vibrio cholerae/isolation & purification ; Vibrio cholerae/pathogenicity ; Vibrio cholerae/radiation effects
    Chemical Substances Agglutinins
    Language English
    Publishing date 1995-10
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    DOI 10.1128/aem.61.10.3656-3660.1995
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 201: Show issues Location:
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  4. Article: Evidence that mortality from Vibrio vulnificus infection results from single strains among heterogeneous populations in shellfish.

    Jackson, J K / Murphree, R L / Tamplin, M L

    Journal of clinical microbiology

    1997  Volume 35, Issue 8, Page(s) 2098–2101

    Abstract: Vibrio vulnificus is the leading cause of food-related mortality reported in the state of Florida. It is normal microflora in marine environments, where seawater and molluscan shellfish are the primary vectors of V. vulnificus disease. Risk correlates ... ...

    Abstract Vibrio vulnificus is the leading cause of food-related mortality reported in the state of Florida. It is normal microflora in marine environments, where seawater and molluscan shellfish are the primary vectors of V. vulnificus disease. Risk correlates with seasonally high numbers of V. vulnificus bacteria during the summer months. Currently, the infectious dose for humans, as well as whether the disease is caused by single or multiple strains found in molluscan shellfish, is unknown. In this work, we studied pulsed-field gel electrophoresis profiles of V. vulnificus strains isolated from blood and oysters associated with V. vulnificus disease. Results showed that ca. 10(3) V. vulnificus bacteria/gram of oyster and higher concentrations were associated with human infections and that a single V. vulnificus strain, evidenced by pulsed-field gel electrophoresis profiles, was isolated from human tissues.
    MeSH term(s) Adult ; Animals ; DNA, Bacterial/analysis ; Electrophoresis, Gel, Pulsed-Field ; Female ; Humans ; Lethal Dose 50 ; Male ; Middle Aged ; Ostreidae/microbiology ; Shellfish/microbiology ; Vibrio/genetics ; Vibrio/pathogenicity ; Vibrio Infections/microbiology ; Vibrio Infections/mortality
    Chemical Substances DNA, Bacterial
    Language English
    Publishing date 1997-08
    Publishing country United States
    Document type Case Reports ; Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 390499-4
    ISSN 1098-660X ; 0095-1137
    ISSN (online) 1098-660X
    ISSN 0095-1137
    DOI 10.1128/jcm.35.8.2098-2101.1997
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1188: Show issues Location:
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  5. Article: Uptake and retention of Vibrio cholerae O1 in the eastern oyster, Crassostrea virginica

    Murphree, R.L / Tamplin, M.L

    Applied and environmental microbiology. Oct 1995. v. 61 (10)

    1995  

    Abstract: Vibrio cholerae O1, the causative agent of cholera, is known to persist in estuarine environments as endogenous microflora. The recent introduction of V. cholerae O1 into estuaries of the North and South American continents has stimulated the need to ... ...

    Abstract Vibrio cholerae O1, the causative agent of cholera, is known to persist in estuarine environments as endogenous microflora. The recent introduction of V. cholerae O1 into estuaries of the North and South American continents has stimulated the need to determine the effect of controlled purification on reducing this pathogen in edible molluscan shellfish. Experiments defined parameters for the uptake and retention of V. cholerae O1 in tissues of Crassostrea virginica, and these parameters were compared with those for Escherichia coli and Salmonella tallahassee, bacteria which are usually eliminated from moderately contaminated shellfish within 48 h. Oysters accumulated greater concentrations of V. cholerae O1 than E. coli and S. tallahassee. When V. cholerae O1 was exposed to controlled purification at 15, 19, and 25 degrees C over 48 h, it persisted in oysters at markedly higher levels than E. coli and S. tallahassee. The concentration of a V. cholerae O1-specific agglutinin did not positively correlate with the uptake or retention of V. cholerae O1. These data show that state and federally approved controlled purification techniques are not effective at reducing V. cholerae O1 in oysters.
    Keywords Crassostrea virginica ; Vibrio cholerae ; Escherichia coli ; Salmonella ; microbial contamination ; decontamination ; ultraviolet radiation ; agglutinins
    Language English
    Dates of publication 1995-10
    Size p. 3656-3660.
    Document type Article
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 2498: Show issues

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  6. Article: Somatic changes in the fetus. ORO-672.

    Murphree, R L

    ORO [reports]. U.S. Atomic Energy Commission

    1969  , Page(s) 3–11

    MeSH term(s) Animals ; Animals, Newborn ; Cobalt Isotopes ; Congenital Abnormalities/etiology ; Eye Abnormalities ; Female ; Fetus/radiation effects ; Gestational Age ; Leg/abnormalities ; Pregnancy ; Radiation Effects ; Rats ; Sheep ; Swine
    Chemical Substances Cobalt Isotopes
    Language English
    Publishing date 1969-10
    Publishing country United States
    Document type Journal Article
    ISSN 0272-4774
    ISSN 0272-4774
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Effect of total body irradiation on rabbit pituitary as measured by gonadotropin response in chicks.

    LANE, J J / PAYSINGER, J R / MURPHREE, R L / RUST, J H / TRUM, B F

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)

    2003  Volume 86, Issue 1, Page(s) 36–38

    MeSH term(s) Animals ; Chickens ; Cobalt ; Cobalt Radioisotopes ; Gonadotropins ; Gonadotropins, Pituitary ; Pituitary Diseases ; Pituitary Gland/radiation effects ; Rabbits ; Radiation Effects ; Radioactivity ; Whole-Body Irradiation
    Chemical Substances Cobalt Radioisotopes ; Gonadotropins ; Gonadotropins, Pituitary ; Cobalt (3G0H8C9362)
    Language English
    Publishing date 2003-09-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 4015-0
    ISSN 1535-3699 ; 1525-1373 ; 0037-9727
    ISSN (online) 1535-3699 ; 1525-1373
    ISSN 0037-9727
    DOI 10.3181/00379727-86-21004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ua VI Zs.111: Show issues Location:
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  8. Article: Prenatal studies. Somatic changes in the fetus. ORO-653.

    Murphree, R L

    ORO [reports]. U.S. Atomic Energy Commission

    1968  , Page(s) 5–8

    MeSH term(s) Abortion, Spontaneous/etiology ; Animals ; Animals, Newborn/radiation effects ; Bone and Bones/abnormalities ; Female ; Limb Deformities, Congenital ; Pregnancy ; Pregnancy, Animal/radiation effects ; Radiation Effects ; Sheep ; Swine
    Language English
    Publishing date 1968-01-01
    Publishing country United States
    Document type Journal Article
    ISSN 0272-4774
    ISSN 0272-4774
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Prenatal studies. Somatic changes in the fetus. ORO-661.

    Murphree, R L

    ORO [reports]. U.S. Atomic Energy Commission

    1967  , Page(s) 4–13

    MeSH term(s) Abortion, Veterinary ; Animals ; Cattle ; Congenital Abnormalities/etiology ; Extremities/radiation effects ; Eye/radiation effects ; Female ; Fetus/radiation effects ; Foot/radiation effects ; Head/radiation effects ; Male ; Neutrons ; Pregnancy ; Rabbits ; Radiation Injuries, Experimental/mortality ; Rats ; Sheep ; Swine ; Tail/radiation effects ; Testis/radiation effects ; Time Factors ; Tooth/radiation effects
    Language English
    Publishing date 1967-07-01
    Publishing country United States
    Document type Journal Article
    ISSN 0272-4774
    ISSN 0272-4774
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: The Borrelia burgdorferi 37-kilodalton immunoblot band (P37) used in serodiagnosis of early lyme disease is the flaA gene product.

    Gilmore, R D / Murphree, R L / James, A M / Sullivan, S A / Johnson, B J

    Journal of clinical microbiology

    1999  Volume 37, Issue 3, Page(s) 548–552

    Abstract: The 37-kDa protein (P37) of Borrelia burgdorferi is an antigen that elicits an early immunoglobulin M (IgM) antibody response in Lyme disease patients. The P37 gene was cloned from a B. burgdorferi genomic library by screening with antibody from a Lyme ... ...

    Abstract The 37-kDa protein (P37) of Borrelia burgdorferi is an antigen that elicits an early immunoglobulin M (IgM) antibody response in Lyme disease patients. The P37 gene was cloned from a B. burgdorferi genomic library by screening with antibody from a Lyme disease patient who had developed a prominent humoral response to the P37 antigen. DNA sequence analysis of this clone revealed the identity of P37 to be FlaA, an outer sheath protein of the periplasmic flagella. Recombinant P37 expression was accomplished in Escherichia coli by using a gene construct with the leader peptide deleted and fused to a 38-kDa E. coli protein. The recombinant antigen was reactive in IgM immunoblots using serum samples from patients clinically diagnosed with early Lyme disease that had been scored positive for B. burgdorferi anti-P37 reactivity. Lyme disease patient samples serologically negative for the B. burgdorferi P37 protein did not react with the recombinant. Recombinant P37 may be a useful component of a set of defined antigens for the serodiagnosis of early Lyme disease. This protein can be utilized as a marker in diagnostic immunoblots, aiding in the standardization of the present generation of IgM serologic tests.
    MeSH term(s) Antibodies, Bacterial/blood ; Borrelia burgdorferi Group/classification ; Borrelia burgdorferi Group/genetics ; Borrelia burgdorferi Group/isolation & purification ; Cloning, Molecular ; DNA Primers ; Flagellin/biosynthesis ; Flagellin/genetics ; Flagellin/immunology ; Glycoproteins/genetics ; Humans ; Immunoglobulin M/blood ; Lyme Disease/blood ; Lyme Disease/diagnosis ; Lyme Disease/immunology ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/immunology ; Reference Values ; Serologic Tests/methods ; Syphilis/blood ; Syphilis/immunology ; Syphilis/microbiology
    Chemical Substances Antibodies, Bacterial ; DNA Primers ; Glycoproteins ; Immunoglobulin M ; Recombinant Proteins ; Flagellin (12777-81-0) ; flaA protein, bacteria (133606-66-3)
    Language English
    Publishing date 1999-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 390499-4
    ISSN 1098-660X ; 0095-1137
    ISSN (online) 1098-660X
    ISSN 0095-1137
    DOI 10.1128/JCM.37.3.548-552.1999
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1188: Show issues Location:
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