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  1. Article ; Online: Application of indocyanine green lymphography combined with methylene blue staining in lymphaticovenular anastomosis of lower limb vessels: A prospective study.

    Zhou, Xuchuan / Liu, Bin / Guo, Xiaowei / Tang, Yueling / Ma, Gejia

    Phlebology

    2023  Volume 38, Issue 7, Page(s) 466–473

    Abstract: Background: Methylene blue (MB) is a conventional lymphatic tracer. We evaluated the application of indocyanine green (ICG) lymphography combined with MB staining in lower limb lymphaticovenular anastomosis (LVA).: Methods: A total of 49 patients ... ...

    Abstract Background: Methylene blue (MB) is a conventional lymphatic tracer. We evaluated the application of indocyanine green (ICG) lymphography combined with MB staining in lower limb lymphaticovenular anastomosis (LVA).
    Methods: A total of 49 patients with lower limb lymphedema were selected as the study subjects and divided into the research (
    Results: The number of anastomotic lymphatic vessels in the study group was higher than that in the control group (
    Conclusion: The circumference of the affected limb is reduced after LVA in patients with lower extremity lymphedema with a favorable prognosis. ICG lymphography combined with MB staining has the advantages of real-time visualization and accurate localization.
    MeSH term(s) Humans ; Indocyanine Green ; Lymphography/methods ; Prospective Studies ; Methylene Blue ; Microsurgery/methods ; Lower Extremity/diagnostic imaging ; Lower Extremity/surgery ; Lymphedema/diagnostic imaging ; Lymphedema/surgery ; Staining and Labeling ; Anastomosis, Surgical/methods ; Lymphatic Vessels/diagnostic imaging ; Lymphatic Vessels/surgery
    Chemical Substances Indocyanine Green (IX6J1063HV) ; Methylene Blue (T42P99266K)
    Language English
    Publishing date 2023-06-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 645172-x
    ISSN 1758-1125 ; 0268-3555
    ISSN (online) 1758-1125
    ISSN 0268-3555
    DOI 10.1177/02683555231185449
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2538: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Application of complete decongestive therapy after lymphaticovenular anastomosis of the lower limb combined with liposuction-A retrospective study research.

    Zhou, Xuchuan / Ma, Gejia / Qi, Xi / Qin, Aoshuang / Liu, Bin

    Phlebology

    2023  Volume 39, Issue 1, Page(s) 49–57

    Abstract: Background: Complete decongestive therapy (CDT) is a conservative treatment for lymphedema that combines interventions and lifestyle changes. We evaluated the application of CDT after lymphaticovenular anastomosis (LVA) of the lower limb combined with ... ...

    Abstract Background: Complete decongestive therapy (CDT) is a conservative treatment for lymphedema that combines interventions and lifestyle changes. We evaluated the application of CDT after lymphaticovenular anastomosis (LVA) of the lower limb combined with liposuction.
    Methods: A total of 55 patients who underwent LVA of the lower limb combined with liposuction from January 2021 to July 2022 were enrolled in the study. The patients were divided into groups A (
    Results: At 6-month and 12-month follow-up, LELI and the score of Lymph-ICF-LL in group B and group A were lower than before the operation (
    Conclusion: This study preliminarily proves the effectiveness of CDT after LVA of the lower limb combined with liposuction, which can maintain and strengthen the surgical effect. Further, CDT treatment is still needed after the operation, which is necessary to reduce the circumference of the affected limb and improve patients' quality of life.
    MeSH term(s) Humans ; Retrospective Studies ; Quality of Life ; Lipectomy ; Lower Extremity/surgery ; Lymphedema/surgery ; Anastomosis, Surgical ; Lymphatic Vessels/surgery ; Treatment Outcome
    Language English
    Publishing date 2023-10-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 645172-x
    ISSN 1758-1125 ; 0268-3555
    ISSN (online) 1758-1125
    ISSN 0268-3555
    DOI 10.1177/02683555231209056
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2538: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Resurfacing partial nose defects with a retroauricular skin/cartilage free flap.

    Han, Yan / Pan, Yong / Yang, Li / Ai, Yufeng / Ma, Gejia / Xia, Wensen / Zhang, Hui

    Annals of plastic surgery

    2011  Volume 67, Issue 1, Page(s) 34–39

    Abstract: Background: The retroauricular skin has always received much attention by plastic surgeons for use in nose reconstruction because of its rich vascularity. Anatomically, the skin of this area lies hidden behind the ear and is very similar to that of the ... ...

    Abstract Background: The retroauricular skin has always received much attention by plastic surgeons for use in nose reconstruction because of its rich vascularity. Anatomically, the skin of this area lies hidden behind the ear and is very similar to that of the face. More importantly, the retroauricular skin flap along with the cartilage can be used to repair defective cartilage of the nose. All these reasons make this area an ideal donor site for nose reconstruction. This article presents the surgical procedures for reconstruction of partial nasal defects with the use of retroauricular skin/cartilage free flaps.
    Methods: Based on the anatomy and blood supply of the retroauricular region, a flap was designed and harvested with the retroauricular vessels as its pedicle. This flap was used to repair the partial nose defects in 10 patients, between the years 2002 and 2008. Of these, 4 patients presented with unilateral ala defects; 2 with nasal tip defects; 2 with defects on the nasal tip and bilateral ala; 1 with unilateral ala and tip defect; and 1 with unilateral ala and lateral defect. The size of the defect ranged from 2.5 × 1.5 cm to 4.0 × 3.0 cm. The flap varied between 3.0 × 2.0 cm and 4.5 × 3.5 cm. In all cases, the auricular cartilage of 1.5 × 1.0 cm to 1.0 × 0.5 cm was taken along with the flap. The retroauricular vessels were anastomosed with the facial vessels. The donor site was covered with full-thickness skin graft or local flap.
    Results: No flap failure was recorded. Excellent morphologic reconstruction was obtained with these flaps with no sequela at the donor site in terms of form and function. Postoperatively, the blood supply to the flaps was fairly good in 8 cases, although there was vein congestion in 2 cases on the fourth day. However, the postoperative results of the 10 cases were satisfactory after treatment.
    Conclusion: The retroauricular skin-cartilage free flap is a good choice for partial nasal reconstruction, having the merits of a well-hidden donor site, good color match, and 1-stage operation for cartilage support and nose reconstruction.
    MeSH term(s) Adult ; Ear Auricle/transplantation ; Female ; Free Tissue Flaps ; Humans ; Male ; Middle Aged ; Rhinoplasty/methods
    Language English
    Publishing date 2011-07
    Publishing country United States
    Document type Evaluation Studies ; Journal Article
    ZDB-ID 423835-7
    ISSN 1536-3708 ; 0148-7043
    ISSN (online) 1536-3708
    ISSN 0148-7043
    DOI 10.1097/SAP.0b013e3181d50e80
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1419: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: The effects of vasonatrin peptide on random pattern skin flap survival.

    Wang, Shi-Ping / Lan, Zhi-Yong / Xia, Wei / Zhao, Xi / Ma, Ge-Jia / Liu, Bei / Pan, Bao-Hua / Guo, Shu-Zhong

    Annals of plastic surgery

    2014  Volume 72, Issue 1, Page(s) 94–99

    Abstract: Background: A lot of methods have been intensively investigated to improve random skin flap survival. Decreasing inflammation and alleviating tissue injury have been reported to be effective in improving survival ratio. Vasonatrin peptide (VNP) is a ... ...

    Abstract Background: A lot of methods have been intensively investigated to improve random skin flap survival. Decreasing inflammation and alleviating tissue injury have been reported to be effective in improving survival ratio. Vasonatrin peptide (VNP) is a chimera of atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP). The current study demonstrates that VNP possesses the venodilating actions of CNP, the natriuretic actions of ANP, and the unique arterial vasodilating actions not associated with either ANP or CNP. However, its effects on skin flap survival have not been previously reported.
    Methods: Sprague-Dawley rats, weighing 220 to 260 g, were randomly divided into 2 groups, namely, the VNP-treated group and the control group. Rectangular random dorsal skin flaps measuring 3 × 9 cm including the panniculus carnosus were elevated, then the flaps were sutured into their original places. In the VNP group, 0.1 mg/kg of VNP was administered intravenously (IV) after surgery and then daily for 3 days. In the control group, 1 mL/kg of saline was administered IV after surgery and then daily for 3 days. To observe the effects of VNP, blood perfusion, histopathological examination, the inflammatory mediators (tumor necrosis factor α, interleukin 1β, and interferon γ), and biochemical analysis (malondialdehyde, glutathione, and myeloperoxidase) were detected and the flap viability was evaluated 7 days after surgery by measuring necrotic flap area and total flap area.
    Results: The viability measurements showed the percentage of flap survival was increased in the VNP-treated group (76.53% ± 6.36%) as compared with the control group (61.12% ± 4.92%) (P < 0.05), and the histological and biochemical assays corroborated the data. The blood perfusion of flaps in the VNP-treated group was higher than the control group (P < 0.05). The inflammatory mediators (tumor necrosis factor α, interleukin 1β, and interferon γ) were significantly lower in the VNP-treated group than the control group (P < 0.05).
    Conclusions: This study found that VNP, which could elevate the tissue blood perfusion and mitigate the tissue damage and inflammatory reaction, is associated with a higher percentage of survival random pattern skin flap area.
    MeSH term(s) Administration, Intravenous ; Animals ; Atrial Natriuretic Factor/pharmacology ; Atrial Natriuretic Factor/therapeutic use ; Biomarkers/metabolism ; Drug Administration Schedule ; Graft Survival/drug effects ; Graft Survival/physiology ; Immunohistochemistry ; Laser-Doppler Flowmetry ; Natriuretic Agents/pharmacology ; Natriuretic Agents/therapeutic use ; Necrosis/etiology ; Necrosis/metabolism ; Necrosis/pathology ; Necrosis/prevention & control ; Postoperative Complications/metabolism ; Postoperative Complications/pathology ; Postoperative Complications/prevention & control ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Skin/blood supply ; Skin/drug effects ; Skin/metabolism ; Skin/pathology ; Surgical Flaps/blood supply ; Surgical Flaps/pathology ; Surgical Flaps/physiology ; Vasodilator Agents/pharmacology ; Vasodilator Agents/therapeutic use
    Chemical Substances Biomarkers ; Natriuretic Agents ; Vasodilator Agents ; ventricular natriuretic peptide, eel (135493-52-6) ; Atrial Natriuretic Factor (85637-73-6)
    Language English
    Publishing date 2014-01
    Publishing country United States
    Document type Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 423835-7
    ISSN 1536-3708 ; 0148-7043
    ISSN (online) 1536-3708
    ISSN 0148-7043
    DOI 10.1097/SAP.0b013e318255a3eb
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1419: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    ab Jg. 2022: Lesesaal (EG)

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  5. Article: [Establishment of new vessels model by implantation of VEGF165 in mouse].

    Xie, Fang / Yang, Li / Tang, Su-Yang / Zheng, Yan / Yi, Cheng-Gang / Xia, Wei / Pan, Yong / Ma, Ge-Jia / Yang, Yang

    Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery

    2011  Volume 27, Issue 5, Page(s) 373–375

    Abstract: Objective: To investigate the feasibility of establishing a murine new vessels model with Lentiviral vector (LVs) mediated human vascular endothelial growth factor-165 (pcDNA3.1/ VEGF165) gene.: Methods: Lentivirus plasmid carrying a human VEGF165 ... ...

    Abstract Objective: To investigate the feasibility of establishing a murine new vessels model with Lentiviral vector (LVs) mediated human vascular endothelial growth factor-165 (pcDNA3.1/ VEGF165) gene.
    Methods: Lentivirus plasmid carrying a human VEGF165 was constructed and was used to transfect mouse's NIH/3T3 cells. The NIH/3T3 cells with high secretion of VEGF were injected into the skeletal muscle of mouse to establish a mouse new vessels model by implantation of vascular endothelium growth factor (VEGF) gene. The external secretion of VEGF was measured with ELISA. Histological examination was carried out after injection. The expression of CD31 was assessed with immunohistochemical method.
    Results: The lenti-VEGF165-EGFP was correctly constructed and confirmed by restriction endonuclease analysis, polymerase chain reaction and DNA sequencing analysis. Lentivirus plasmid carrying a human VEGF165 was constructed. lenti-VEGF165-EGFP was used to transfect mouse's NIH/ 3T3 cells, and human VEGF165 gene was assessed. NIH/3T3 cells mediated VEGF gene implantation can produce stable and effective mouse new vessels model. The external secretion of VEGF in peripheral blood was measured with ELISA. The legs became swollen in experimental group 14 d after injection. It found the cells expression of CD31 44 d after injection, and histological analysis showed the swollen tissue was composed of small new vessels.
    Conclusions: NIH/3T3 cells mediated VEGF gene implantation can produce stable and effective mouse new vessels model.
    MeSH term(s) Animals ; Disease Models, Animal ; Genetic Vectors ; Humans ; Mice ; Mice, Inbred C57BL ; NIH 3T3 Cells ; Neovascularization, Pathologic ; Plasmids ; Transfection ; Vascular Endothelial Growth Factor A/genetics ; Vascular Endothelial Growth Factor A/metabolism
    Chemical Substances VEGFA protein, human ; Vascular Endothelial Growth Factor A
    Language Chinese
    Publishing date 2011-09
    Publishing country China
    Document type English Abstract ; Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2019799-8
    ISSN 1009-4598 ; 1000-7806
    ISSN 1009-4598 ; 1000-7806
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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    ab Jg. 2022: Lesesaal (EG)

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