Article ; Online: MiRNA-584 suppresses the progression of ovarian cancer by negatively regulating LPIN1.
European review for medical and pharmacological sciences
2020 Volume 24, Issue 3, Page(s) 1062–1071
Abstract: Objective: The aim of the study was to detect the expression level of microRNA-584 (miRNA-584) in ovarian cancer (OCa) and to elucidate its regulatory effect on OCa progression by regulating LPIN1.: Patients and methods: Expression levels of miRNA- ... ...
| Abstract | Objective: The aim of the study was to detect the expression level of microRNA-584 (miRNA-584) in ovarian cancer (OCa) and to elucidate its regulatory effect on OCa progression by regulating LPIN1. Patients and methods: Expression levels of miRNA-584 and LPIN1 in 31 matched OCa tissues and paracancerous tissues were detected. The relationship of miRNA-584 level with clinical indicators and prognosis of OCa patients was analyzed. Influences of miRNA-584/LPIN1 regulatory loop on malignant phenotypes of OVCAR3 and PEO1 cells were assessed. In addition, the interaction between miRNA-584 and LPIN1 was confirmed by Dual-Luciferase reporter gene assay and rescue experiments. Results: MiRNA-584 was lowly expressed in OCa tissues, while LPIN1 was highly expressed. OCa patients expressing a low level of miRNA-584 suffered from higher rates of lymphatic metastasis and distant metastasis, as well as worse survival. The overexpression of miRNA-584 in OVCAR3 cells attenuated proliferative and migratory abilities, while the knockdown of miRNA-584 in PEO1 cells yielded the opposite results. LPIN1 was verified to be the target binding to miRNA-584 and its level was negatively regulated by miRNA-584. The overexpression of LPIN1 accelerated OCa cells to proliferate and migrate. Importantly, LPIN1 was responsible for OCa progression regulated by miRNA-584. Conclusions: MiRNA-584 is downregulated in OCa tissues and cell lines. MiRNA-584 level is correlated with lymphatic metastasis, distant metastasis, and poor prognosis in OCa patients. By negatively regulating LPIN1, miRNA-584 suppresses the malignant progression of OCa. |
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| MeSH term(s) | Aged ; Cell Line, Tumor ; Disease Progression ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; MicroRNAs/antagonists & inhibitors ; MicroRNAs/biosynthesis ; MicroRNAs/genetics ; Middle Aged ; Ovarian Neoplasms/metabolism ; Ovarian Neoplasms/pathology ; Ovarian Neoplasms/prevention & control ; Phosphatidate Phosphatase/biosynthesis ; Phosphatidate Phosphatase/genetics |
| Chemical Substances | MIRN584 microRNA, human ; MicroRNAs ; LPIN1 protein, human (EC 3.1.3.4) ; Phosphatidate Phosphatase (EC 3.1.3.4) |
| Language | English |
| Publishing date | 2020-02-24 |
| Publishing country | Italy |
| Document type | Journal Article |
| ZDB-ID | 605550-3 |
| ISSN | 2284-0729 ; 1128-3602 ; 0392-291X |
| ISSN (online) | 2284-0729 |
| ISSN | 1128-3602 ; 0392-291X |
| DOI | 10.26355/eurrev_202002_20156 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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