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  1. Article: Mu-Xiang-You-Fang protects PC12 cells against OGD/R-induced autophagy via the AMPK/mTOR signaling pathway

    Ma, Hui-xia / Chen, Ai-ling / Hou, Fan / Li, Ting-ting / Zhao, Qi-peng / Zhu, Ya-fei

    Journal of ethnopharmacology. 2020 Apr. 24, v. 252

    2020  

    Abstract: Mu-Xiang-You-Fang (MXYF) is a classic prescription of Hui medicine. It is composed of five herbs and has been used to treat ischemic stroke for many years. However, the potential pharmacological mechanisms of MXYF remain unclear. The present research is ... ...

    Abstract Mu-Xiang-You-Fang (MXYF) is a classic prescription of Hui medicine. It is composed of five herbs and has been used to treat ischemic stroke for many years. However, the potential pharmacological mechanisms of MXYF remain unclear. The present research is aimed to investigate the protective effect and possible mechanisms of MXYF treatment in an in vitro model of cerebral ischemia-reperfusion injury.An oxygen-glucose deprivation and reperfusion (OGD/R) model of PC12 cells was established. The effect of MXYF on the cell viability after OGD/R injury was determined using a cell counting kit (CCK-8) assay. The colorimetric method was used to determine the lactate dehydrogenase (LDH) leakage rate. The calcium concentration was determined by the chemical fluorescence method, and mitochondrial membrane potential was determined using flow cytometry. Monodansylcadaverine (MDC) staining and electron microscopic analysis were then conducted to detect autophagy after oxygen-glucose deprivation and reperfusion in PC12 cells. Immunofluorescence and western blot analyses were used to detect the expression of proteins associated with autophagy.It was found that MXYF (1, 2, 4 μg/mL) could significantly increase cell viability and mitochondrial membrane potential and decrease the calcium concentration and LDH release rate in PC12 cells. After OGD/R injury in PC12 cells, the number of autophagosomes and autophagolysosome significantly increased. MXYF (4 μg/mL) inhibited the autophagy induced by OGD/R and inhibited the expression of LC3, beclin1, p-AMPK, and ULK1. In contrast, the expression of p-mTOR, p-p70s6k, and p62 was significantly enhanced.These findings suggest that MXYF inhibits autophagy after OGD/R-induced PC12 cell injury through the AMPK-mTOR pathway. Thus, MXYF might have therapeutic potential in treating ischemic stroke.
    Keywords autophagy ; calcium ; cell viability ; colorimetry ; electron microscopy ; flow cytometry ; fluorescence ; fluorescent antibody technique ; herbs ; lactate dehydrogenase ; membrane potential ; mitochondrial membrane ; models ; protective effect ; proteins ; signal transduction ; staining ; stroke ; therapeutics ; traditional medicine ; Western blotting
    Language English
    Dates of publication 2020-0424
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2020.112583
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 90/710: Show issues

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  2. Article ; Online: Mu-Xiang-You-Fang protects PC12 cells against OGD/R-induced autophagy via the AMPK/mTOR signaling pathway.

    Ma, Hui-Xia / Hou, Fan / Chen, Ai-Ling / Li, Ting-Ting / Zhu, Ya-Fei / Zhao, Qi-Peng

    Journal of ethnopharmacology

    2020  Volume 252, Page(s) 112583

    Abstract: Ethnopharmacological relevance: Mu-Xiang-You-Fang (MXYF) is a classic prescription of Hui medicine. It is composed of five herbs and has been used to treat ischemic stroke for many years. However, the potential pharmacological mechanisms of MXYF remain ... ...

    Abstract Ethnopharmacological relevance: Mu-Xiang-You-Fang (MXYF) is a classic prescription of Hui medicine. It is composed of five herbs and has been used to treat ischemic stroke for many years. However, the potential pharmacological mechanisms of MXYF remain unclear. The present research is aimed to investigate the protective effect and possible mechanisms of MXYF treatment in an in vitro model of cerebral ischemia-reperfusion injury.
    Materials and methods: An oxygen-glucose deprivation and reperfusion (OGD/R) model of PC12 cells was established. The effect of MXYF on the cell viability after OGD/R injury was determined using a cell counting kit (CCK-8) assay. The colorimetric method was used to determine the lactate dehydrogenase (LDH) leakage rate. The calcium concentration was determined by the chemical fluorescence method, and mitochondrial membrane potential was determined using flow cytometry. Monodansylcadaverine (MDC) staining and electron microscopic analysis were then conducted to detect autophagy after oxygen-glucose deprivation and reperfusion in PC12 cells. Immunofluorescence and western blot analyses were used to detect the expression of proteins associated with autophagy.
    Results: It was found that MXYF (1, 2, 4 μg/mL) could significantly increase cell viability and mitochondrial membrane potential and decrease the calcium concentration and LDH release rate in PC12 cells. After OGD/R injury in PC12 cells, the number of autophagosomes and autophagolysosome significantly increased. MXYF (4 μg/mL) inhibited the autophagy induced by OGD/R and inhibited the expression of LC3, beclin1, p-AMPK, and ULK1. In contrast, the expression of p-mTOR, p-p70s6k, and p62 was significantly enhanced.
    Conclusions: These findings suggest that MXYF inhibits autophagy after OGD/R-induced PC12 cell injury through the AMPK-mTOR pathway. Thus, MXYF might have therapeutic potential in treating ischemic stroke.
    MeSH term(s) AMP-Activated Protein Kinases/metabolism ; Animals ; Autophagy/drug effects ; Cell Hypoxia ; Drugs, Chinese Herbal/pharmacology ; Glucose/deficiency ; Neuroprotective Agents/pharmacology ; Oxygen ; PC12 Cells ; Rats ; Reperfusion Injury/drug therapy ; Reperfusion Injury/metabolism ; Signal Transduction/drug effects ; TOR Serine-Threonine Kinases/metabolism
    Chemical Substances Drugs, Chinese Herbal ; Mu-Xiang-You-Fang ; Neuroprotective Agents ; TOR Serine-Threonine Kinases (EC 2.7.1.1) ; mTOR protein, rat (EC 2.7.1.1) ; AMP-Activated Protein Kinases (EC 2.7.11.31) ; Glucose (IY9XDZ35W2) ; Oxygen (S88TT14065)
    Language English
    Publishing date 2020-01-22
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2020.112583
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1494: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Occurrence and Characterization of Dimethachlon Insensitivity in Sclerotinia sclerotiorum in Jiangsu Province of China.

    Ma, Hui-Xia / Feng, Xi-Jie / Chen, Yu / Chen, Chang-Jun / Zhou, Ming-Guo

    Plant disease

    2014  Volume 93, Issue 1, Page(s) 36–42

    Abstract: Sclerotinia stem rot, caused by Sclerotinia sclerotiorum, is the main fungal disease of oilseed rape (Brassica napus) in China. Numerous fungicide applications are required for control. Dimethachlon, one of the dicarboximide fungicides, has been the ... ...

    Abstract Sclerotinia stem rot, caused by Sclerotinia sclerotiorum, is the main fungal disease of oilseed rape (Brassica napus) in China. Numerous fungicide applications are required for control. Dimethachlon, one of the dicarboximide fungicides, has been the major fungicide for disease control after benzimidazole resistance became widespread. Fungal populations were collected throughout Jiangsu Province between 2006 and 2007 in order to determine their sensitivity to dimethachlon. A total of 1,066 single-sclerotium isolates of S. sclerotiorum were collected, and most of the isolates were considered sensitive to dimethachlon. Five isolates collected in Yancheng and Changzhou showed normal growth at 5 μg/ml dimethachlon with the resistance factor ≈10 (resistance factor was estimated as ratios between the EC
    Language English
    Publishing date 2014-06-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-93-1-0036
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 2043: Show issues

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  4. Article: Occurrence and Characterization of Dimethachlon Insensitivity in Sclerotinia sclerotiorum in Jiangsu Province of China

    Ma, Hui-Xia / Feng, Xi-Jie / Chen, Yu / Chen, Chang-Jun / Zhou, Ming-Guo

    Plant disease: an international journal of applied plant pathology. 2009 Jan., v. 93, no. 1

    2009  

    Abstract: Sclerotinia stem rot, caused by Sclerotinia sclerotiorum, is the main fungal disease of oilseed rape (Brassica napus) in China. Numerous fungicide applications are required for control. Dimethachlon, one of the dicarboximide fungicides, has been the ... ...

    Abstract Sclerotinia stem rot, caused by Sclerotinia sclerotiorum, is the main fungal disease of oilseed rape (Brassica napus) in China. Numerous fungicide applications are required for control. Dimethachlon, one of the dicarboximide fungicides, has been the major fungicide for disease control after benzimidazole resistance became widespread. Fungal populations were collected throughout Jiangsu Province between 2006 and 2007 in order to determine their sensitivity to dimethachlon. A total of 1,066 single-sclerotium isolates of S. sclerotiorum were collected, and most of the isolates were considered sensitive to dimethachlon. Five isolates collected in Yancheng and Changzhou showed normal growth at 5 μg/ml dimethachlon with the resistance factor [almost equal to]10 (resistance factor was estimated as ratios between the EC50 values of resistant isolates and the average EC50 values of sensitive ones) compared to the sensitive isolates (EC50 is the concentration of fungicide causing 50% reduction in growth). Through in vitro selection for resistance to the fungicide, 25 dimethachlon-resistant mutants were derived from 10 wild-type isolates of S. sclerotiorum. The resistance factors for the isolates ranged from 198 to 484, and the isolates were considered highly resistant to dimethachlon. Therefore, at least two different mechanisms of resistance seem to be involved: one that may provide a moderate resistance (insensitivity) and a second that may give a high resistance level under laboratory conditions. There was positive cross-resistance between dimethachlon and other dicarboximide fungicides, such as iprodione and procymidone, in these S. sclerotiorum isolates. The field dimethachlon-insensitive and the laboratory-induced dimethachlon-resistant isolates appeared to have mycelial growth, sclerotial production, and pathogenicity comparable to their wild-type parental isolates. Also, results of osmotic tests showed that there were no significant difference in mycelial radial growth between the field dimethachlon-sensitive and field dimethachlon-insensitive isolates on potato dextrose agar plates amended with 2, 4, 6, or 8% (wt/vol) NaCl, but the laboratory-induced dimethachlon-resistant isolates grew significantly more slowly than their wild-type sensitive parents under all concentrations of NaCl. Because these studies yielded a high frequency of laboratory resistance in S. sclerotiorum, together with the occurrence of field insensitivity, appropriate precautions against resistance development in natural populations should be taken.
    Keywords Sclerotinia sclerotiorum ; dicarboximide fungicides ; fungicide resistance ; disease control ; natural selection ; Brassica napus ; disease surveillance ; resistance management ; application rate ; dosage ; in vitro studies ; artificial selection ; cross resistance ; mycelium ; microbial growth ; sclerotia ; pathogenicity ; China
    Language English
    Dates of publication 2009-01
    Size p. 36-42.
    Document type Article
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-93-1-0036
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 2043: Show issues

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  5. Article: Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China

    Wang, Jian-Xin / Ma, Hui-Xia / Chen, Yu / Zhu, Xiao-Fen / Yu, Wen-Yuan / Tang, Zheng-He / Chen, Chang-Jun / Zhou, Ming-Guo

    Crop protection. 2009 Oct., v. 28, no. 10

    2009  

    Abstract: Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron ... ...

    Abstract Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17+/-0.09 μg ml ⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250g ai ha ⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750g ai ha ⁻¹ and dimethachlon (46% WP), at 690g ai ha ⁻¹ with control efficacies of 18.9 and 44.6%, respectively.
    Keywords Sclerotinia sclerotiorum ; fungicides ; fungicide resistance ; mycelium ; microbial growth ; strain differences ; application rate ; carbendazim ; cross resistance ; field experimentation ; China
    Language English
    Dates of publication 2009-10
    Size p. 882-886.
    Publishing place [Amsterdam]: Elsevier Science
    Document type Article
    ZDB-ID 786839-x
    ISSN 0261-2194
    ISSN 0261-2194
    DOI 10.1016/j.cropro.2009.06.012
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 3919: Show issues

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  6. Article: Activity of carbendazim, dimethachlon, iprodione, procymidone and boscalid against Sclerotinia stem rot in Jiangsu Province of China

    Ma, Hui-Xia / Chen, Yu / Wang, Jian-Xin / Yu, Wen-Yuan / Tang, Zheng-He / Chen, Chang-Jun / Zhou, Ming-Guo

    Phytoparasitica. 2009 Nov., v. 37, no. 5

    2009  

    Keywords Brassica napus var. napus ; rapeseed ; fungal diseases of plants ; Sclerotinia sclerotiorum ; plant pathogenic fungi ; pathogenicity ; signs and symptoms (plants) ; leaves ; stems ; pods ; developmental stages ; disease control ; carbendazim ; dimethachlor ; iprodione ; procymidone ; application rate ; mycelium ; cell growth ; sclerotia ; pathotypes ; genetic variation ; China
    Language English
    Dates of publication 2009-11
    Size p. 421-429.
    Document type Article
    ZDB-ID 188372-0
    ISSN 1876-7184 ; 0334-2123
    ISSN (online) 1876-7184
    ISSN 0334-2123
    Database NAL-Catalogue (AGRICOLA)

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