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Article ; Online: Multiplex fluorescent amplification-refractory mutation system PCR method for the detection of 10 genetic defects in Holstein cattle and its comparison with the KASP genotyping assay.

Khan, Md Yousuf Ali / Dai, Dongmei / Su, Xin / Tian, Jia / Zhou, Jiamin / Ma, Liqin / Wang, Yachun / Wen, Wan / Zhang, Yi

Animal genetics

2024 Volume 55, Issue 3, Page(s) 457–464

Abstract: The common deleterious genetic defects in Holstein cattle include haplotypes 1-6 (HH1-HH6), haplotypes for cholesterol deficiency (HCD), bovine leukocyte adhesion deficiency (BLAD), complex vertebral malformation (CVM) and brachyspina syndrome (BS). ... ...

Abstract	The common deleterious genetic defects in Holstein cattle include haplotypes 1-6 (HH1-HH6), haplotypes for cholesterol deficiency (HCD), bovine leukocyte adhesion deficiency (BLAD), complex vertebral malformation (CVM) and brachyspina syndrome (BS). Recessive inheritance patterns of these genetic defects permit the carriers to function normally, but homozygous recessive genotypes cause embryo loss or neonatal death. Therefore, rapid detection of the carriers is essential to manage these genetic defects. This study was conducted to develop a single-tube multiplex fluorescent amplification-refractory mutation system (mf-ARMS) PCR method for efficient genotyping of these 10 genetic defects and to compare its efficiency with the kompetitive allele specific PCR (KASP) genotyping assay. The mf-ARMS PCR method introduced 10 sets of tri-primers optimized with additional mismatches in the 3' end of wild and mutant-specific primers, size differentiation between wild and mutant-specific primers, fluorescent labeling of universal primers, adjustment of annealing temperatures and optimization of primer concentrations. The genotyping of 484 Holstein cows resulted in 16.12% carriers with at least one genetic defect, while no homozygous recessive genotype was detected. This study found carrier frequencies ranging from 0.0% (HH6) to 3.72% (HH3) for individual defects. The mf-ARMS PCR method demonstrated improved detection, time and cost efficiency compared with the KASP method for these defects. Therefore, the application of mf-ARMS PCR for genotyping Holstein cattle is anticipated to decrease the frequency of lethal alleles and limit the transmission of these genetic defects.
MeSH term(s)	Animals ; Cattle/genetics ; Genotyping Techniques/veterinary ; Genotyping Techniques/methods ; Cattle Diseases/genetics ; Multiplex Polymerase Chain Reaction/veterinary ; Genotype ; Polymerase Chain Reaction/veterinary ; Mutation
Language	English
Publishing date	2024-04-15
Publishing country	England
Document type	Journal Article ; Comparative Study
ZDB-ID	632766-7
ISSN	1365-2052 ; 0268-9146 ; 0268-9154
ISSN (online)	1365-2052
ISSN	0268-9146 ; 0268-9154
DOI	10.1111/age.13432
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Article: Sulfosuccinimidyl oleate ameliorates the high-fat diet-induced obesity syndrome by reducing intestinal and hepatic absorption.

Ma, Qiming / Wen, Li / Tian, Yanxia / Ma, Liqin / Wen, Zhangsheng / Kun, Yang / Xu, Mengping / Liu, Xiaoping

Frontiers in pharmacology

2023 Volume 14, Page(s) 1193006

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2023-05-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2023.1193006
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Article ; Online: Genetic parameters for health traits and their association with fertility and milk production in Chinese Holsteins.

Zhang, Junxing / Han, Liyun / Zhang, Hailiang / Hu, Honghong / Sheng, Hui / Yang, Tongtong / Zhang, Yi / Wen, Wan / Ma, Liqin / Ma, Yun / Wang, Yachun

Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie

2023 Volume 141, Issue 1, Page(s) 52–64

Abstract: Herd health is one of the key problems influencing the efficiency of the dairy industry. Genetic selection, with a focus on animal health, is important for herd improvement. This study aimed to estimate genetic parameters for health traits and their ... ...

Abstract	Herd health is one of the key problems influencing the efficiency of the dairy industry. Genetic selection, with a focus on animal health, is important for herd improvement. This study aimed to estimate genetic parameters for health traits and their correlations with fertility and milk production traits in dairy cattle. Based on records from 58,549 lactating cows calved between 2015 and 2021, a total of 24 health traits (six composite health traits and 18 independent health traits), four fertility traits and five milk production traits were analysed. First, linear and threshold animal models were used to estimate the variance components and heritabilities of the health traits. Second, a bivariate linear animal model was used to estimate genetic correlations among all 24 health traits. Finally, a bivariate linear animal model based on records from the first lactation was used to estimate the correlations between health traits and fertility or milk production traits. The results showed that all health traits had low heritabilities, ranging from 0.002 (0.001) to 0.048 (0.004) in the linear model and from <0.001 (0.021) to 0.226 (0.035) in the threshold model. Genetic correlations between health traits across categories were generally low, whereas the relatively high genetic correlations were found between health traits within the same category. In this study, only a few significant and moderate genetic correlations were observed between health traits and fertility or milk production traits. Clinical mastitis showed relatively moderate correlations with fertility traits, ranging from 0.277 (0.113) (interval from first to last insemination) to 0.401 (0.104) (calving interval). Moreover, there were moderate genetic correlations between hoof health and milk production traits. The results from the current study will support balanced dairy breeding to genetically improve disease resistance in dairy cows.
MeSH term(s)	Female ; Cattle/genetics ; Animals ; Lactation/genetics ; Milk ; Fertility/genetics ; Phenotype ; Cattle Diseases/genetics
Language	English
Publishing date	2023-09-25
Publishing country	Germany
Document type	Journal Article
ZDB-ID	631363-2
ISSN	1439-0388 ; 0044-3581 ; 0931-2668 ; 1742-4488
ISSN (online)	1439-0388
ISSN	0044-3581 ; 0931-2668 ; 1742-4488
DOI	10.1111/jbg.12825
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Article ; Online: Application of a treatment planning system-assisted large-aperture computed tomography simulator to percutaneous biopsy: initial experience of a radiation therapist.

Lin, Xiaoyi / Ma, Liqin / Du, Kaixin / Hong, Junqiang / Luo, Shuiying / Lai, Youqun / Dai, Yongliang / Kong, Xiangquan

The Journal of international medical research

2021 Volume 49, Issue 1, Page(s) 300060520983141

Abstract: Objective: To evaluate the application of treatment planning system (TPS)-assisted large-aperture computed tomography (CT) simulator to percutaneous biopsy.: Methods: This retrospective study enrolled patients that underwent TPS-assisted large- ... ...

Abstract	Objective: To evaluate the application of treatment planning system (TPS)-assisted large-aperture computed tomography (CT) simulator to percutaneous biopsy. Methods: This retrospective study enrolled patients that underwent TPS-assisted large-aperture CT simulator-guided percutaneous biopsy from November 2018 to December 2019. Retrospective analyses of puncture accuracy were compared using paired Results: A total of 38 patients were included in this study. There were no significant differences between the planned and actual puncture depth and angle. Pulmonary puncture was significantly associated with the accuracy of the puncture angle. The diagnostic rate of malignancy was 76% (29 of 38), of which 20 of 25 patients were in the group initially diagnosed with unconfirmed lesions and nine of 13 patients were in the group of treated patients that needed additional pathological analyses. For patients that underwent a pulmonary biopsy, 12 had minor pneumothorax and three suffered needle track bleeding. No other complications were observed. Regression analyses indicated a significant correlation between puncture angle and the incidence of pneumothorax. Conclusion: TPS-assisted large-aperture CT simulator may improve the percutaneous biopsy procedure by combining the advantages of radiotherapy specialties with computer targeting.
MeSH term(s)	Humans ; Image-Guided Biopsy ; Lung/diagnostic imaging ; Lung Neoplasms ; Pneumothorax ; Retrospective Studies ; Tomography, X-Ray Computed
Language	English
Publishing date	2021-01-20
Publishing country	England
Document type	Journal Article
ZDB-ID	184023-x
ISSN	1473-2300 ; 0300-0605 ; 0142-2596
ISSN (online)	1473-2300
ISSN	0300-0605 ; 0142-2596
DOI	10.1177/0300060520983141
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Article: Is it necessary for clinical tumor volume including neck muscles in target volume delineation of nasopharyngeal carcinoma?

Chen, Fei / Kong, Xiangquan / Wu, Haixia / Fang, Weining / Fei, Zhaodong / Wu, Zhupeng / Zhao, Dan / Ma, Liqin

Laryngoscope investigative otolaryngology

2021 Volume 6, Issue 6, Page(s) 1353–1357

Abstract: Objectives: To calculate the shrinkage of the neck muscles and dosimetric changes and to clarify the necessity of covering part of the muscle in neck node region delineation for patients with nasopharyngeal carcinoma (NPC) treated with intensity- ... ...

Abstract	Objectives: To calculate the shrinkage of the neck muscles and dosimetric changes and to clarify the necessity of covering part of the muscle in neck node region delineation for patients with nasopharyngeal carcinoma (NPC) treated with intensity-modulated radiotherapy. Methods: In total, 44 patients with NPC were enrolled. Distances between the lateral border of the neck muscles and longitudinal midline were measured on every selected slice. This process was repeated three times, and the mean values of the three distances of planning computed tomography (CT) images and repeated CT images were adopted (labeled Results: The median Conclusion: Patients with NPC experienced medial shrinkage of the neck muscles by 1.5 mm, and the consequent dose variation was negligible. It is unnecessary to cover part of the muscles in the delineation of the CTV-n.
Language	English
Publishing date	2021-11-05
Publishing country	United States
Document type	Journal Article
ISSN	2378-8038
ISSN	2378-8038
DOI	10.1002/lio2.691
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Article: Which evaluation criteria of the short-term efficacy can better reflect the long-term outcomes for patients with nasopharyngeal carcinoma?

Ma, Li-Qin / Wu, Hai-Xia / Kong, Xiang-Quan / Fei, Zhao-Dong / Fang, Wei-Ning / Du, Kai-Xin / Chen, Fei / Zhao, Dan / Wu, Zhu-Peng

Translational oncology

2022 Volume 20, Page(s) 101412

Abstract: Purpose: To compare the consistency of one-dimensional Response Evaluation Criteria in Solid Tumors (1D-RECIST), two-dimensional WHO criteria (2D-WHO), and three-dimensional (3D) measurement for therapeutic response assessment of nasopharyngeal ... ...

Abstract	Purpose: To compare the consistency of one-dimensional Response Evaluation Criteria in Solid Tumors (1D-RECIST), two-dimensional WHO criteria (2D-WHO), and three-dimensional (3D) measurement for therapeutic response assessment of nasopharyngeal carcinoma (NPC). Materials and methods: Retrospective data of 288 newly diagnosed NPC patients were reviewed. Tumor size was assessed on magnetic resonance imaging (MRI) according to the 1D-RECIST, 2D-WHO, and 3D measurement criteria. Agreement between tumor responses was assessed using unweighted k statistics. The receiver operating characteristic (ROC) curve was used to determine the optimal cut-off point of the PTV. The Kaplan-Meier method and Cox regression were used for the survival analysis. Results: The optimal cut-off point of the PTV for progression-free survival (PFS) was 29.6%. Agreement with PTV measurement was better for 1D measurement than for 2D and 3D measurements (kappa values of 0.646, 0.537, and 0.577 for 1D, 2D, and 3D measurements, respectively; P < 0.05). The area under the curve of the 1D measurement (AUC=0.596) was similar to that of the PTV measurement (AUC=0.621). Compared with 2D and 3D measurements, 1D measurement is superior for predicting prognosis in NPC (C-index of 0.672, 0.663, and 0.646 were for 1D, 2D, and 3D measurements, respectively; P < 0.005). Survival analysis showed that patients with non-responders had worse prognosis (P < 0.05). Conclusions: The 1D measurement more closely agreed with the PTV measurement than the 2D and 3D measurements for predicting therapeutic responses in NPC. Therefore, we recommend using the less time-consuming 1D-RECIST criteria in routine clinical practice.
Language	English
Publishing date	2022-04-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	2443840-6
ISSN	1936-5233
ISSN	1936-5233
DOI	10.1016/j.tranon.2022.101412
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Article: Outcomes and Experiences of Child-Bearing Women with Nasopharyngeal Carcinoma.

Ma, Liqin / Chen, Fei / Kong, Xiangquan / Xu, Ting / Fei, Zhaodong / Fang, Weining / Wang, Binyi / Wu, Haixia

Cancer management and research

2020 Volume 12, Page(s) 8047–8054

Abstract: Purpose: Nasopharyngeal carcinoma (NPC) is more common among women in Southeast Asia. An important issue is whether it is safe for them to bear children after treatment and when it is safe to do so. We conducted this study to explore the relation ... ...

Abstract	Purpose: Nasopharyngeal carcinoma (NPC) is more common among women in Southeast Asia. An important issue is whether it is safe for them to bear children after treatment and when it is safe to do so. We conducted this study to explore the relation between fertility and prognosis in child-bearing women with NPC. Patients and methods: Child-bearing women were defined as young women between the ages of 18 and 30. A total of 127 eligible child-bearing NPC patients were identified from December 2003 to December 2014. The patients were divided into two groups, depending on whether or not they had post-therapeutic births. The Kaplan-Meier method was used for survival analyses. The Log rank test was used to compare two survival curves and the independent significances of different prognostic factors were assessed by Cox proportional hazards regression analysis. Results: The 5-year overall survival (OS) and disease-free survival (DFS) in the Childbirth group were significantly higher than those in the Non-Childbirth group (100% vs 88.8%, P = 0.026 and 100% vs 77.5%, P = 0.007, respectively). In the Childbirth group, no difference was found in the 5-year DFS between different birth interval times, from 1 to 5 years after treatment. The clinical stage was identified as the risk factor of OS (HR = 101.725, 95% CI: 2.160-4790.910, P = 0.019), and consequent childbirth after treatment was associated with favorable DFS (HR = 0.148, 95% CI: 0.034-0.643, P = 0.011). Conclusion: Post-therapeutic birth did not increase the mortality risk of child-bearing women with NPC. There was no significant correlation between the subsequent birth time window after treatment and the prognosis.
Language	English
Publishing date	2020-09-04
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2508013-1
ISSN	1179-1322
ISSN	1179-1322
DOI	10.2147/CMAR.S265371
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Article ; Online: Corrigendum: Increased Duodenal Eosinophil Degranulation in Patients with Functional Dyspepsia: A Prospective Study.

Du, Lijun / Shen, Jinhua / Kim, John J / Yu, Yunxian / Ma, Liqin / Dai, Ning

Scientific reports

2017 Volume 7, Page(s) 46121

Language	English
Publishing date	2017-04-07
Publishing country	England
Document type	Journal Article ; Published Erratum
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/srep46121
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Article ; Online: Increased Duodenal Eosinophil Degranulation in Patients with Functional Dyspepsia: A Prospective Study.

Du, Lijun / Shen, Jinhua / Kim, John J / Yu, Yunxian / Ma, Liqin / Dai, Ning

Scientific reports

2016 Volume 6, Page(s) 34305

Abstract: Functional dyspepsia (FD) is a functional gastrointestinal disorder diagnosed by symptom-based criteria. It has been said that duodenal immune activation plays a role in the pathogenesis of FD. The primary aims of the study were to compare the total ... ...

Abstract	Functional dyspepsia (FD) is a functional gastrointestinal disorder diagnosed by symptom-based criteria. It has been said that duodenal immune activation plays a role in the pathogenesis of FD. The primary aims of the study were to compare the total number of duodenal eosinophil and evaluate the eosinophil degranulation rate, number of duodenal degranulated eosinophil and mast cell between patients with FD and healthy subjects. We enrolled 96 patients with FD and 24 healthy controls at Sir Run Run Shaw Hospital. The total number of eosinophil was comparable in the second portion of duodenum (D2) and duodenal bulb (D1) between patients with FD and healthy controls (all P > 0.05). Significant higher eosinophil degranulation positive rate in D2 (P = 0.003) and a trend towards higher in D1 (P = 0.084) were observed in patients with FD compared with healthy controls. Moreover, the number of duodenal degranulated eosinophil in patients with FD were significantly increased than healthy controls in D1(9.8 ± 6.3 vs 2.9 ± 2.1 per HPF, P = 0.0002) and a trend towards increase in D2 (10.7 ± 7.7 vs 5.3 ± 0.9 per HPF, P = 0.077), respectively. However, degranulated mast cells in patients with FD were almost same with healthy controls. Increased eosinophils degranulation in duodenum play an important role in pathogenesis of FD.
MeSH term(s)	Adult ; Cell Degranulation ; Duodenum/metabolism ; Duodenum/pathology ; Dyspepsia/metabolism ; Dyspepsia/pathology ; Eosinophils/metabolism ; Eosinophils/pathology ; Female ; Humans ; Male ; Mast Cells/metabolism ; Mast Cells/pathology ; Middle Aged ; Prospective Studies
Language	English
Publishing date	2016-10-06
Publishing country	England
Document type	Clinical Trial ; Comparative Study ; Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/srep34305
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Article: [Risk factors of contrast-induced nephropathy after percutaneous coronary intervention in patients with coronary heart disease and the countermeasures].

Zheng, Hua / Tu, Yan / Ma, Li-qin

Nan fang yi ke da xue xue bao = Journal of Southern Medical University

2011 Volume 31, Issue 7, Page(s) 1273–1276

Abstract: Objective: To identify the risk factors of contrast-induced nephropathy (CIN) after coronary interventional therapy (PCI) in patients with coronary heart disease and analyze the clinical outcomes and the preventive measures of CIN.: Methods: Ninety- ... ...

Abstract	Objective: To identify the risk factors of contrast-induced nephropathy (CIN) after coronary interventional therapy (PCI) in patients with coronary heart disease and analyze the clinical outcomes and the preventive measures of CIN. Methods: Ninety-one patients who developed CIN after PCI were retrospectively analyzed to identify the risk factors and explore the preventive measures. Results: CIN was strongly associated with pre-procedural chronic renal failure, diabetes mellitus and administration of large-dose contrast. The incidence of cardiac mortality in hospital or in the follow-up at one year after PCI, and the incidence of myocardial infarction or major adverse cardiac events in the follow-up at one year were obviously higher in patients with CIN than those without CIN. Conclusion: Chronic renal failure, diabetes mellitus and large-dose contrast administration are 3 independent risk factors of CIN, which affects the prognosis of the patients. Reinforcement of a comprehensive perioperative management of PCI, especially a rigorous preoperative preparation, can be an important strategy for prevention of CIN.
MeSH term(s)	Aged ; China/epidemiology ; Contrast Media/adverse effects ; Coronary Disease/therapy ; Female ; Humans ; Kidney Diseases/chemically induced ; Kidney Diseases/epidemiology ; Kidney Diseases/prevention & control ; Male ; Middle Aged ; Percutaneous Coronary Intervention/adverse effects ; Retrospective Studies ; Risk Factors
Chemical Substances	Contrast Media
Language	Chinese
Publishing date	2011-06
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	2250951-3
ISSN	1673-4254
ISSN	1673-4254
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