LIVIVO - Search results -

Search results

Result 1 - 8 of total 8

Search options

Article ; Online: Sediments from Arctic Tide-Water Glaciers Remove Coastal Marine Viruses and Delay Host Infection.

Maat, Douwe S / Prins, Maarten A / Brussaard, Corina P D

2019 Volume 11, Issue 2

Abstract: Over the past few decades, the Arctic region has been strongly affected by global warming, leading to increased sea surface temperatures and melting of land and sea ice. Marine terminating (tide-water) glaciers are expected to show higher melting and ... ...

Abstract	Over the past few decades, the Arctic region has been strongly affected by global warming, leading to increased sea surface temperatures and melting of land and sea ice. Marine terminating (tide-water) glaciers are expected to show higher melting and calving rates, with an increase in the input of fine sediment particles in the coastal marine environment. We experimentally investigated whether marine viruses, which drive microbial interactions and biogeochemical cycling are removed from the water column through adsorption to glacier-delivered fine sediments. Ecologically relevant concentrations of 30, 100 and 200 mg·L
MeSH term(s)	Arctic Regions ; Chlorophyta/virology ; Geologic Sediments/virology ; Global Warming ; Haptophyta/virology ; Ice Cover/virology ; Microbial Consortia ; Phytoplankton/virology ; Virus Attachment ; Water Microbiology
Language	English
Publishing date	2019-01-30
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v11020123
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article: Virus removal by glacier-derived suspended fine sediment in the Arctic

Maat, Douwe S / Visser, Ronald J.W / Brussaard, Corina P.D

Journal of experimental marine biology and ecology. 2019 Sept. 07,

2019

Abstract: Viruses are a major source of mortality for phytoplankton and bacteria and are therefore seen as drivers of food web dynamics and biogeochemical cycling in the marine pelagic environment. Previous studies have shown that aquatic viruses adsorb to ... ...

Abstract	Viruses are a major source of mortality for phytoplankton and bacteria and are therefore seen as drivers of food web dynamics and biogeochemical cycling in the marine pelagic environment. Previous studies have shown that aquatic viruses adsorb to suspended sediment, which theoretically decreases the mortality pressure on their microbial hosts. This process is of particular ecological importance in the Arctic, where coastal systems contain large amounts of suspended fine-sediment, supplied by melting and calving glaciers. The aim of this study was to investigate the effects of glacier-derived fine sediment on marine Arctic microbes during summer in Storfjorden, Svalbard (78°N, 20°E). We sampled for microbial abundances over transects with increasing sediment concentration towards three different glaciers, and examined the adsorption of the natural virus community to previously collected glacier-derived sediment. Our data show declined abundances of phytoplankton (<20 μm) and bacteria towards all 3 glaciers. Viral abundances, however, showed an even stronger decline with the virus to bacterium ratio (VBR) reducing from 10 to 16 in open water to 3–6 in the vicinity of the glaciers. Linear regressions showed negative linear relationships of VBR with turbidity and sediment. This negative relation between suspended sediment and Arctic marine virus abundances is further confirmed by very high adsorption rates of in situ Arctic marine virioplankton upon addition of glacier sediment. Sediment additions (of ecologically relevant concentrations of 100, 200 and 500 mg L−1 to natural seawater) caused viral losses varying between 38 and 66% of the total virus community. Such high viral losses translate into lower contact rates between host and virus, reducing host mortality. Sediment inflow through glaciers may thus affect marine pelagic food web dynamics via viruses, possibly altering the main flow of carbon and other elements in the process. Further study to the possible consequences for food web structure and biogeochemical cycling is essential, as Arctic glacier-derived sediment inflow does not only fluctuate seasonally but is also expected to increase with global warming.
Keywords	adsorption ; bacteria ; carbon ; food webs ; glaciers ; mortality ; phytoplankton ; seawater ; sediments ; summer ; suspended sediment ; turbidity ; viruses ; Arctic region ; Norway
Language	English
Dates of publication	2019-0907
Publishing place	Elsevier B.V.
Document type	Article
Note	NAL-AP-2-clean ; Pre-press version
ZDB-ID	410283-6
ISSN	0022-0981
ISSN	0022-0981
DOI	10.1016/j.jembe.2019.151227
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 74/703: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Influence of Irradiance and Temperature on the Virus MpoV-45T Infecting the Arctic Picophytoplankter

Piedade, Gonçalo J / Wesdorp, Ella M / Montenegro-Borbolla, Elena / Maat, Douwe S / Brussaard, Corina P D

Viruses

2018 Volume 10, Issue 12

Abstract: Arctic marine ecosystems are currently undergoing rapid changes in temperature and light availability. Picophytoplankton, such ... ...

Abstract	Arctic marine ecosystems are currently undergoing rapid changes in temperature and light availability. Picophytoplankton, such as
MeSH term(s)	Arctic Regions ; Chlorophyta/metabolism ; Chlorophyta/virology ; Climate Change ; Host-Pathogen Interactions ; Light ; Photosynthesis ; Plant Viruses/physiology ; Plant Viruses/radiation effects ; Radiation ; Temperature ; Virus Replication
Language	English
Publishing date	2018-11-29
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v10120676
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Virus production in phosphorus-limited Micromonas pusilla stimulated by a supply of naturally low concentrations of different phosphorus sources, far into the lytic cycle.

Maat, Douwe S / van Bleijswijk, Judith D L / Witte, Harry J / Brussaard, Corina P D

FEMS microbiology ecology

2016 Volume 92, Issue 9

Abstract: Earlier studies show that the proliferation of phytoplankton viruses can be inhibited by depletion of soluble reactive phosphorus (SRP; orthophosphate). In natural marine waters, phytoplankton phosphorus (P) availability is, however, largely determined ... ...

Abstract	Earlier studies show that the proliferation of phytoplankton viruses can be inhibited by depletion of soluble reactive phosphorus (SRP; orthophosphate). In natural marine waters, phytoplankton phosphorus (P) availability is, however, largely determined by the supply rate of SRP (e.g. through remineralization) and potentially by the source of P as well (i.e. the utilization of soluble non-reactive P; SNP). Here we show how a steady low supply of P (mimicking natural P recycling) to virally infected P-limited Micromonas pusilla stimulates virus proliferation. Independent of the degree of P limitation prior to infection (0.32 and 0.97μmax chemostat cultures), SRP supply resulted in 2-fold higher viral burst sizes (viruses lysed per host cell) as compared with no addition (P starvation). Delaying these spikes during the infection cycle showed that the added SRP was utilized for extra M. pusilla virus (MpV) production far into the lytic cycle (18 h post-infection). Moreover, P-limited M. pusilla utilized several SNP compounds with high efficiency and with the same extent of burst size stimulation as for SRP. Finally, addition of virus-free MpV lysate (representing a complex SNP mixture) to newly infected cells enhanced MpV production, implicating host-associated alkaline phosphatase activity, and highlighting its important role in oligotrophic environments.
MeSH term(s)	Chlorophyta/metabolism ; Chlorophyta/virology ; Phosphates/metabolism ; Phosphorus/metabolism ; Phytoplankton/metabolism ; Phytoplankton/virology ; Virus Physiological Phenomena
Chemical Substances	Phosphates ; Phosphorus (27YLU75U4W)
Language	English
Publishing date	2016-09
Publishing country	England
Document type	Journal Article
ZDB-ID	283722-5
ISSN	1574-6941 ; 0168-6496
ISSN (online)	1574-6941
ISSN	0168-6496
DOI	10.1093/femsec/fiw136
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2967: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Virus production in phosphorus-limited Micromonas pusilla stimulated by a supply of naturally low concentrations of different phosphorus sources, far into the lytic cycle

Maat, Douwe S / van Bleijswijk, Judith D. L / Witte, Harry J / Brussaard, Corina P. D

FEMS microbiology ecology. 2016 June 16, v. 92, no. 9

2016

Abstract	Earlier studies show that the proliferation of phytoplankton viruses can be inhibited by depletion of soluble reactive phosphorus (SRP; orthophosphate). In natural marine waters, phytoplankton phosphorus (P) availability is, however, largely determined by the supply rate of SRP (e.g. through remineralization) and potentially by the source of P as well (i.e. the utilization of soluble non-reactive P; SNP). Here we show how a steady low supply of P (mimicking natural P recycling) to virally infected P-limited Micromonas pusilla stimulates virus proliferation. Independent of the degree of P limitation prior to infection (0.32 and 0.97μₘₐₓ chemostat cultures), SRP supply resulted in 2-fold higher viral burst sizes (viruses lysed per host cell) as compared with no addition (P starvation). Delaying these spikes during the infection cycle showed that the added SRP was utilized for extra M. pusilla virus (MpV) production far into the lytic cycle (18 h post-infection). Moreover, P-limited M. pusilla utilized several SNP compounds with high efficiency and with the same extent of burst size stimulation as for SRP. Finally, addition of virus-free MpV lysate (representing a complex SNP mixture) to newly infected cells enhanced MpV production, implicating host-associated alkaline phosphatase activity, and highlighting its important role in oligotrophic environments.
Keywords	alkaline phosphatase ; enzyme activity ; orthophosphates ; phytoplankton ; reactive phosphorus ; single nucleotide polymorphism ; starvation ; viruses
Language	English
Dates of publication	2016-0616
Publishing place	Oxford University Press
Document type	Article
ZDB-ID	283722-5
ISSN	1574-6941 ; 0168-6496
ISSN (online)	1574-6941
ISSN	0168-6496
DOI	10.1093/femsec/fiw136
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 2967: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Elevated CO2 and phosphate limitation favor Micromonas pusilla through stimulated growth and reduced viral impact.

Maat, Douwe S / Crawfurd, Katherine J / Timmermans, Klaas R / Brussaard, Corina P D

Applied and environmental microbiology

2014 Volume 80, Issue 10, Page(s) 3119–3127

Abstract: Growth and viral infection of the marine picoeukaryote Micromonas pusilla was studied under a future-ocean scenario of elevated partial CO2 (pCO2; 750 μatm versus the present-day 370 μatm) and simultaneous limitation of phosphorus (P). Independent of the ...

Abstract	Growth and viral infection of the marine picoeukaryote Micromonas pusilla was studied under a future-ocean scenario of elevated partial CO2 (pCO2; 750 μatm versus the present-day 370 μatm) and simultaneous limitation of phosphorus (P). Independent of the pCO2 level, the ratios of M. pusilla cellular carbon (C) to nitrogen (N), C:P and N:P, increased with increasing P stress. Furthermore, in the P-limited chemostats at growth rates of 0.32 and 0.97 of the maximum growth rate (μmax), the supply of elevated pCO2 led to an additional rise in cellular C:N and C:P ratios, as well as a 1.4-fold increase in M. pusilla abundance. Viral lysis was not affected by pCO2, but P limitation led to a 150% prolongation of the latent period (6 to 12 h) and an 80% reduction in viral burst sizes (63 viruses per cell) compared to P-replete conditions (4 to 8 h latent period and burst size of 320). Growth at 0.32 μmax further prolonged the latent period by another 150% (12 to 18 h). Thus, enhanced P stress due to climate change-induced strengthened vertical stratification can be expected to lead to reduced and delayed virus production in picoeukaryotes. This effect is tempered, but likely not counteracted, by the increase in cell abundance under elevated pCO2. Although the influence of potential P-limitation-relieving factors, such as the uptake of organic P and P utilization during infection, is unclear, our current results suggest that when P limitation prevails in future oceans, picoeukaryotes and grazing will be favored over larger-sized phytoplankton and viral lysis, with increased matter and nutrient flow to higher trophic levels.
MeSH term(s)	Carbon Dioxide/metabolism ; Chlorophyta/chemistry ; Chlorophyta/growth & development ; Chlorophyta/metabolism ; Chlorophyta/virology ; Climate Change ; Kinetics ; Nitrogen/metabolism ; Phosphates/metabolism ; Phosphorus/metabolism ; Virus Physiological Phenomena
Chemical Substances	Phosphates ; Carbon Dioxide (142M471B3J) ; Phosphorus (27YLU75U4W) ; Nitrogen (N762921K75)
Language	English
Publishing date	2014-03-07
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	223011-2
ISSN	1098-5336 ; 0099-2240
ISSN (online)	1098-5336
ISSN	0099-2240
DOI	10.1128/AEM.03639-13
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 201: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Characterization and Temperature Dependence of Arctic Micromonas polaris Viruses.

Maat, Douwe S / Biggs, Tristan / Evans, Claire / van Bleijswijk, Judith D L / van der Wel, Nicole N / Dutilh, Bas E / Brussaard, Corina P D

Viruses

2017 Volume 9, Issue 6

Abstract: Global climate change-induced warming of the Artic seas is predicted to shift the phytoplankton community towards dominance of smaller-sized species due to global warming. Yet, little is known about their viral mortality agents despite the ecological ... ...

Abstract	Global climate change-induced warming of the Artic seas is predicted to shift the phytoplankton community towards dominance of smaller-sized species due to global warming. Yet, little is known about their viral mortality agents despite the ecological importance of viruses regulating phytoplankton host dynamics and diversity. Here we report the isolation and basic characterization of four prasinoviruses infectious to the common Arctic picophytoplankter
MeSH term(s)	Arctic Regions ; Chlorophyta/virology ; DNA, Viral/genetics ; Phycodnaviridae/genetics ; Phycodnaviridae/physiology ; Phycodnaviridae/radiation effects ; Temperature ; Virus Replication/radiation effects
Chemical Substances	DNA, Viral
Language	English
Publishing date	2017-06-02
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v9060134
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Book ; Online: Increasing P limitation and viral infection impact lipid remodeling of the picophytoplankter Micromonas pusilla

Maat, Douwe S. / Bale, Nicole J. / Hopmans, Ellen C. / Sinninghe Damsté, Jaap S. / Schouten, Stefan / Brussaard, Corina P. D.

eISSN: 1726-4189

2016

Abstract: The intact polar lipid (IPL) composition of phytoplankton is plastic and dependent on environmental factors. Previous studies have shown that phytoplankton under low phosphorus (P) availability substitutes phosphatidylglycerols (PGs) with ... ...

Abstract	The intact polar lipid (IPL) composition of phytoplankton is plastic and dependent on environmental factors. Previous studies have shown that phytoplankton under low phosphorus (P) availability substitutes phosphatidylglycerols (PGs) with sulfoquinovosyldiacylglycerols (SQDGs) and digalactosyldiacylglycerols (DGDGs). However, these studies focused merely on P depletion, while phytoplankton in the natural environment often experience P limitation whereby the strength depends on the supply rate of the limiting nutrient. Here we report on the IPL composition of axenic cultures of the picophotoeukaryote Micromonas pusilla under different degrees of P limitation, i.e., P-controlled chemostats at 97 and 32 % of the maximum growth rate, and P starvation (obtained by stopping P supply to these chemostats). P-controlled cultures were also grown at elevated partial carbon dioxide pressure ( p CO 2 ) to mimic a future scenario of strengthened vertical stratification in combination with ocean acidification. Additionally, we tested the influence of viral infection for this readily infected phytoplankton host species. Results show that both SQDG : PG and DGDG : PG ratios increased with enhanced P limitation. Lipid composition was, however, not affected by enhanced (750 vs. 370 µatm) p CO 2 . In the P-starved virally infected cells the increase in SQDG : PG and DGDG : PG ratios was lower, whereby the extent depended on the growth rate of the host cultures before infection. The lipid membrane of the virus MpV-08T itself lacked some IPLs (e.g., monogalactosyldiacylglycerols; MGDGs) in comparison with its host. This study demonstrates that, besides P concentration, also the P supply rate, viral infection and even the history of the P supply rate can affect phytoplankton lipid composition (i.e., the non-phospholipid : phospholipid ratio), with possible consequences for the nutritional quality of phytoplankton.
Subject code	570
Language	English
Publishing date	2016-03-17
Publishing country	de
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED