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  1. Article: Data on SVCT2 transporter expression and localization in cancer cell lines and tissues

    Roa, Francisco J. / Peña, Eduardo / Inostroza, Eveling / Sotomayor, Kirsty / González, Mauricio / Gutierrez-Castro, Francisco A. / Maurin, Michelle / Sweet, Karen / Labrousse, Claire / Gatica, Marcell / Aylwin, Carlos F. / Mendoza, Pamela / Maldonado, Mafalda / Delgado, Carolina / Madariaga, Jaime / Panes, Jessica / Silva-Grecchi, Tiare / Concha, Ilona I. / Moraga-Cid, Gustavo /
    Reyes, Alejandro M. / Muñoz-Montesino, Carola / Vera, Juan Carlos / Rivas, Coralia I.

    Data in Brief. 2019 Aug., v. 25

    2019  

    Abstract: The data presented in this article are related to the research paper entitled “Increased expression of mitochondrial sodium-coupled ascorbic acid transporter-2 (mitSVCT2) as a central feature in breast cancer”, available in Free Radical Biology and ... ...

    Abstract The data presented in this article are related to the research paper entitled “Increased expression of mitochondrial sodium-coupled ascorbic acid transporter-2 (mitSVCT2) as a central feature in breast cancer”, available in Free Radical Biology and Medicine Journal [1]. In this article, we examined the SVCT2 transporter expression in various breast cancer cell lines using RT-PCR and Western blot assays. In addition, we analyzed the subcellular localization of SVCT2 by immunofluorescence colocalization assays and cellular fractionation experiments. Finally, an analysis of different cancer tissue microarrays immunostained for SVCT2 and imaged by The Human Protein Atlas (https://www.proteinatlas.org) is presented.
    Keywords Western blotting ; ascorbic acid ; breast neoplasms ; fluorescent antibody technique ; fractionation ; mitochondria ; neoplasm cells
    Language English
    Dates of publication 2019-08
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 2786545-9
    ISSN 2352-3409
    ISSN 2352-3409
    DOI 10.1016/j.dib.2019.103972
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Data on SVCT2 transporter expression and localization in cancer cell lines and tissues.

    Roa, Francisco J / Peña, Eduardo / Inostroza, Eveling / Sotomayor, Kirsty / González, Mauricio / Gutierrez-Castro, Francisco A / Maurin, Michelle / Sweet, Karen / Labrousse, Claire / Gatica, Marcell / Aylwin, Carlos F / Mendoza, Pamela / Maldonado, Mafalda / Delgado, Carolina / Madariaga, Jaime / Panes, Jessica / Silva-Grecchi, Tiare / Concha, Ilona I / Moraga-Cid, Gustavo /
    Reyes, Alejandro M / Muñoz-Montesino, Carola / Vera, Juan Carlos / Rivas, Coralia I

    Data in brief

    2019  Volume 25, Page(s) 103972

    Abstract: The data presented in this article are related to the research paper entitled "Increased expression of mitochondrial sodium-coupled ascorbic acid transporter-2 (mitSVCT2) as a central feature in breast cancer", available in Free Radical Biology and ... ...

    Abstract The data presented in this article are related to the research paper entitled "Increased expression of mitochondrial sodium-coupled ascorbic acid transporter-2 (mitSVCT2) as a central feature in breast cancer", available in Free Radical Biology and Medicine Journal [1]. In this article, we examined the SVCT2 transporter expression in various breast cancer cell lines using RT-PCR and Western blot assays. In addition, we analyzed the subcellular localization of SVCT2 by immunofluorescence colocalization assays and cellular fractionation experiments. Finally, an analysis of different cancer tissue microarrays immunostained for SVCT2 and imaged by The Human Protein Atlas (https://www.proteinatlas.org) is presented.
    Language English
    Publishing date 2019-05-06
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2786545-9
    ISSN 2352-3409 ; 2352-3409
    ISSN (online) 2352-3409
    ISSN 2352-3409
    DOI 10.1016/j.dib.2019.103972
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Increased expression of mitochondrial sodium-coupled ascorbic acid transporter-2 (mitSVCT2) as a central feature in breast cancer.

    Peña, Eduardo / Roa, Francisco J / Inostroza, Eveling / Sotomayor, Kirsty / González, Mauricio / Gutierrez-Castro, Francisco A / Maurin, Michelle / Sweet, Karen / Labrousse, Claire / Gatica, Marcell / Aylwin, Carlos F / Mendoza, Pamela / Maldonado, Mafalda / Delgado, Carolina / Madariaga, Jaime / Panes, Jessica / Silva-Grecchi, Tiare / Concha, Ilona I / Moraga-Cid, Gustavo /
    Reyes, Alejandro M / Muñoz-Montesino, Carola / Vera, Juan Carlos / Rivas, Coralia I

    Free radical biology & medicine

    2019  Volume 135, Page(s) 283–292

    Abstract: The potential role of vitamin C in cancer prevention and treatment remains controversial. While normal human cells obtain vitamin C as ascorbic acid, the prevalent form of vitamin C in vivo, the uptake mechanisms by which cancer cells acquire vitamin C ... ...

    Abstract The potential role of vitamin C in cancer prevention and treatment remains controversial. While normal human cells obtain vitamin C as ascorbic acid, the prevalent form of vitamin C in vivo, the uptake mechanisms by which cancer cells acquire vitamin C has remained unclear. The aim of this study is to characterize how breast cancer cells acquire vitamin C. For this, we determined the expression of vitamin C transporters in normal and breast cancer tissue samples, and in ZR-75, MCF-7, MDA-231 and MDA-468 breast cancer cell lines. At the same time, reduced (AA) and oxidized (DHA) forms of vitamin C uptake experiments were performed in all cell lines. We show here that human breast cancer tissues differentially express a form of SVCT2 transporter, that is systematically absent in normal breast tissues and it is increased in breast tumors. In fact, estrogen receptor negative breast cancer tissue, exhibit the most elevated SVCT2 expression levels. Despite this, our analysis in breast cancer cell lines showed that these cells are not able to uptake ascorbic acid and depend on glucose transporter for the acquisition of vitamin C by a bystander effect. This is consistent with our observations that this form of SVCT2 is completely absent from the plasma membrane and is overexpressed in mitochondria of breast cancer cells, where it mediates ascorbic acid transport. This work shows that breast cancer cells acquire vitamin C in its oxidized form and are capable of accumulated high concentrations of the reduced form. Augmented expression of an SVCT2 mitochondrial form appears to be a common hallmark across all human cancers and might have implications in cancer cells survival capacity against pro-oxidant environments.
    MeSH term(s) Ascorbic Acid/metabolism ; Breast Neoplasms/genetics ; Breast Neoplasms/pathology ; Bystander Effect ; Female ; Gene Expression Regulation, Neoplastic/genetics ; Humans ; MCF-7 Cells ; Mitochondria/genetics ; Mitochondria/pathology ; Mitochondrial Membrane Transport Proteins/genetics ; Oxidation-Reduction ; Reactive Oxygen Species/metabolism ; Sodium/metabolism ; Sodium-Coupled Vitamin C Transporters/genetics
    Chemical Substances Mitochondrial Membrane Transport Proteins ; Reactive Oxygen Species ; SLC23A2 protein, human ; Sodium-Coupled Vitamin C Transporters ; Sodium (9NEZ333N27) ; Ascorbic Acid (PQ6CK8PD0R)
    Language English
    Publishing date 2019-03-19
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 807032-5
    ISSN 1873-4596 ; 0891-5849
    ISSN (online) 1873-4596
    ISSN 0891-5849
    DOI 10.1016/j.freeradbiomed.2019.03.015
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2109: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  4. Article: Cirugía transesfenoidal en enfermedad de Cushing durante gestación.

    Verdugo, Cecilia / Alegría, Jorge / Grant, Carlos / Briano, Elena / González, María I / Meza, Héctor / Amthauer, Nancy / Paiva, Oriana / Vigueras, Rogelio / Madariaga, Jaime

    Revista medica de Chile

    2004  Volume 132, Issue 1, Page(s) 75–80

    Abstract: We report a 26 year old female with Cushing's disease who became pregnant during the course of her disease. She was treated by transsphenoidal surgery during the second trimester of pregnancy, achieving resolution of hypercortisolism. She had a normal ... ...

    Title translation Cushing's disease treatment with transsphenoidal surgery during pregnancy.
    Abstract We report a 26 year old female with Cushing's disease who became pregnant during the course of her disease. She was treated by transsphenoidal surgery during the second trimester of pregnancy, achieving resolution of hypercortisolism. She had a normal delivery at term, her offspring was healthy, and she had a normal lactation. Currently, five years after surgery, she is asymptomatic. According to our literature review, this patient is the fifth reported case of pituitary adenomectomy during gestation.
    MeSH term(s) Adenoma/complications ; Adenoma/surgery ; Adult ; Cushing Syndrome/etiology ; Cushing Syndrome/surgery ; Female ; Humans ; Infant, Newborn ; Pituitary Neoplasms/complications ; Pituitary Neoplasms/surgery ; Pregnancy ; Pregnancy Complications, Neoplastic/surgery ; Sphenoid Sinus/surgery
    Language Spanish
    Publishing date 2004-06-30
    Publishing country Chile
    Document type Case Reports ; English Abstract ; Journal Article ; Review
    ZDB-ID 732136-3
    ISSN 0717-6163 ; 0034-9887
    ISSN (online) 0717-6163
    ISSN 0034-9887
    DOI 10.4067/s0034-98872004000100012
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 402: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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