Article: Analysis of hypomorphic KitlSl mutants suggests different requirements for KITL in proliferation and migration of mouse primordial germ cells.
2005 Volume 73, Issue 4, Page(s) 639–647
Abstract: Germ cell development in mice is initiated when a small number of primordial germ cells (PGCs) are set aside from somatic cells during gastrulation. In the subsequent 4 to 5 days, PGCs enter the hindgut, undergo a directed migration away from the hindgut ...
| Abstract | Germ cell development in mice is initiated when a small number of primordial germ cells (PGCs) are set aside from somatic cells during gastrulation. In the subsequent 4 to 5 days, PGCs enter the hindgut, undergo a directed migration away from the hindgut into the developing gonads, and undergo a massive increase in cell number. It is well established that Kit ligand (KITL, also known as stem cell factor and mast cell growth factor) is required for the survival and proliferation of PGCs. However, there is little information on a direct role for KITL in PGC migration. By comparing the effects of multiple Kitl mutations, including two N-ethyl-N-nitrosourea-induced hypomorphic mutations, we were able to distinguish stages of PGC development that are preferentially affected by certain mutations. We provide evidence that the requirements for KITL in proliferation are different in PGCs before and after they start migrating, and different levels of KITL function are required to support PGC proliferation and migration. This study illustrates the usefulness of an allelic series of mutations to dissect developmental processes and suggests that these mutants may be useful for further studies of molecular mechanisms of KITL functions in gametogenesis. |
|---|---|
| MeSH term(s) | Animals ; Cell Count ; Cell Movement/genetics ; Cell Proliferation ; Ethylnitrosourea/toxicity ; Female ; Male ; Mice ; Mice, Mutant Strains ; Mutation ; Ovary/cytology ; Ovary/embryology ; Ovum/cytology ; Ovum/metabolism ; Spermatozoa/cytology ; Spermatozoa/metabolism ; Stem Cell Factor/drug effects ; Stem Cell Factor/genetics ; Stem Cell Factor/metabolism ; Testis/cytology ; Testis/embryology |
| Chemical Substances | Stem Cell Factor ; Ethylnitrosourea (P8M1T4190R) |
| Language | English |
| Publishing date | 2005-10 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S. |
| ZDB-ID | 1118-6 |
| ISSN | 1529-7268 ; 0006-3363 |
| ISSN (online) | 1529-7268 |
| ISSN | 0006-3363 |
| DOI | 10.1095/biolreprod.105.042846 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 551: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.