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  1. Article ; Online: Iran's commercial herbal products for managing COVID-19

    Vahideh Ahmadpour / Saba Rahimi Bahoosh / Parvin Jahanbani / Mahdieh Eftekhari

    Phytomedicine Plus, Vol 3, Iss 4, Pp 100494- (2023)

    A review based on clinical trial-based approach

    2023  

    Abstract: Background: Despite the existence of treatment strategies, there was no effective drug treatment available during the outbreak of COVID-19. Consequently, medicinal herbs were commonly used as complementary drugs to prevent COVID-19 and reduce the ... ...

    Abstract Background: Despite the existence of treatment strategies, there was no effective drug treatment available during the outbreak of COVID-19. Consequently, medicinal herbs were commonly used as complementary drugs to prevent COVID-19 and reduce the symptoms of the disease in many countries. Medicinal plants used in the preparation of anti-COVID-19 supplements have proven effective in reducing the symptoms of this disease, with various mechanisms such as strengthening the immune system, antiviral effects, and anti-inflammatory activities. Several herbal products based on traditional and complementary medicine have been used worldwide for the treatment of COVID-19 infection. Examples include Chinese herbal medicines such as Jinhua Qinggan and Lianhua Qingwen, Indian Kadha used as an immune booster and, Artemisia annua, which gained popularity in African countries like Madagascar during the COVID-19 pandemic. Latin American and Caribbean regions, such as Cuba, have utilized 'Prevengho-VIR' to prevent severe respiratory symptoms of COVID-19. Purpose: This study was conducted with the aim of introducing Iran's commercial herbal products for the management of COVID-19 based on clinical trials. Methods: Research articles based on clinical evidence of Iran's commercial herbal supplements for managing COVID-19 were collected from Google Scholar, PubMed, and ScienceDirect databases. In addition, the anti-COVID potential of medicinal plants used in these herbal products has also been discussed. Results: This study presents a comprehensive review of the 12 approved COVID-19 herbal supplements in Iran, based on related articles published so far that discuss their clinical use. The Food and Drug Administration of Iran has approved the use of commercial herbal products, including Alerguard®, Imfluna®, Palminavir®, Co Iran Amin®, PHR 160®, Colchinale®, Thymonige®, Covexirpars®, Mocozift, Saliravira®, Relung ZMZ®, and Ferula astragalus ZMZ®. These products are produced with herbal medicine and are intended for the symptomatic ...
    Keywords COVID-19 ; Commercial ; Herbal products ; Clinical Trial ; Iran ; Other systems of medicine ; RZ201-999
    Subject code 610
    Language English
    Publishing date 2023-11-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Phytochemical analysis and anticholinesterase activity of aril of Myristica fragrans Houtt

    Arezoo Rastegari / Azadeh Manayi / Mahdi Rezakazemi / Mahdieh Eftekhari / Mahnaz Khanavi / Tahmineh Akbarzadeh / Mina Saeedi

    BMC Chemistry, Vol 16, Iss 1, Pp 1-

    2022  Volume 9

    Abstract: Abstract In this study, the ethyl acetate fraction of Myristica fragrans Houtt. was investigated for its in vitro anticholinesterase activity as well as neuroprotectivity against H2O2-induced cell death in PC12 neuronal cells and the ability to chelate ... ...

    Abstract Abstract In this study, the ethyl acetate fraction of Myristica fragrans Houtt. was investigated for its in vitro anticholinesterase activity as well as neuroprotectivity against H2O2-induced cell death in PC12 neuronal cells and the ability to chelate bio-metals (Zn2+, Fe2+, and Cu2+). The fraction was inactive toward acetylcholinesterase (AChE); however, it inhibited the butyrylcholinesterase (BChE) with IC50 value of 68.16 µg/mL, compared with donepezil as the reference drug (IC50 = 1.97 µg/mL) via Ellman’s method. It also showed good percentage of neuroprotection (86.28% at 100 µg/mL) against H2O2-induced neurotoxicity and moderate metal chelating ability toward Zn2+, Fe2+, and Cu2+. The phytochemical study led to isolation and identification of malabaricone A (1), malabaricone C (2), 4-(4-(3,4-dimethoxyphenyl)-2,3-dimethylbutyl)benzene-1,2-diol (3), nectandrin B (4), macelignan (5), and 4-(4-(benzo[d][1,3]dioxol-5-yl)-1-methoxy-2,3-dimethylbutyl)-2-methoxyphenol (6) which were assayed for their cholinesterase (ChE) inhibitory activity. Compounds 1 and 3 were not previously reported for M. fragrans. Among isolated compounds, compound 2 showed the best activity toward both AChE and BChE with IC50 values of 25.02 and 22.36 μM, respectively, compared with donepezil (0.07 and 4.73 μM, respectively).
    Keywords AChE ; BChE ; Mace ; Metal chelating ; Myristica fragrans Houtt ; Neuroprotectivity ; Chemistry ; QD1-999
    Subject code 540
    Language English
    Publishing date 2022-11-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Severity of oxidative stress as a hallmark in COVID-19 patients

    Alireza Bastin / Fatemeh Abbasi / Narges Roustaei / Jahangir Abdesheikhi / Hossein Karami / Mohammad Gholamnezhad / Mahdieh Eftekhari / Amirhossein Doustimotlagh

    European Journal of Medical Research, Vol 28, Iss 1, Pp 1-

    2023  Volume 10

    Abstract: Abstract Introduction Understanding the mechanisms and identifying effective treatments for the COVID-19 outbreak are imperative. Therefore, this study aimed to assess the antioxidant status and oxidative stress parameters as potential pivotal mechanisms ...

    Abstract Abstract Introduction Understanding the mechanisms and identifying effective treatments for the COVID-19 outbreak are imperative. Therefore, this study aimed to assess the antioxidant status and oxidative stress parameters as potential pivotal mechanisms in asymptomatic, non-severe, and severe COVID-19 patients. Methods This study is a case–control study that was performed on patients referred to the Persian Gulf Martyrs Hospital of Bushehr University of Medical Sciences, Bushehr, Iran, from May 2021 to September 2021. A total of 600 COVID-19 patients (non-severe and severe group) and 150 healthy volunteers of the same age and sex were selected during the same period. On the first day of hospitalization, 10 ml of venous blood was taken from subjects. Then, hematological, biochemical, serological, antioxidant and oxidative stress parameters were determined. Results Our results indicated that ESR, CRP, AST, ALT, and LDH significantly augmented in the severe group as compared to the non-severe and normal groups (P ≤ 0.05). It was observed that the levels of FRAP, G6PD activity, and SOD activity significantly reduced in the non-severe patients in comparison with the severe and normal groups (P ≤ 0.05). We found that MDA content and NO metabolite markedly increased in severe patients as compared to the non-severe group. Conclusions Taken together, it seems that the balance between antioxidants and oxidants was disturbed in COVID-19 patients in favor of oxidant markers. In addition, this situation caused more aggravation in severe patients as compared to the non-severe group.
    Keywords COVID-19 ; Oxidative stress ; Antioxidant ; MDA ; SOD ; G6PD ; Medicine ; R
    Subject code 610
    Language English
    Publishing date 2023-12-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Correction

    Mina Saeedi / Aida Iraji / Yasaman Vahedi-Mazdabadi / Atiyeh Alizadeh / Najmeh Edraki / Omidreza Firuzi / Mahdieh Eftekhari / Tahmineh Akbarzadeh

    BMC Complementary Medicine and Therapies, Vol 22, Iss 1, Pp 1-

    Cinnamomum verum J. Presl. Bark essential oil: in vitro investigation of anti-cholinesterase, anti-BACE1, and neuroprotective activity

    2022  Volume 1

    Keywords Other systems of medicine ; RZ201-999
    Language English
    Publishing date 2022-11-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Antioxidant and Anti-Inflammatory Effects of Origanum majorana L. Methanolic Extract on Bile Duct Ligation in Male Rats

    Izadpanah Gheitasi / Nikta Motaghi / Hossein Sadeghi / Heibatollah Sadeghi / Zahra Moslemi / Mahdieh Eftekhari / Nasrin Shakerinasab / Amir Hossein Doustimotlagh

    Evidence-Based Complementary and Alternative Medicine, Vol

    2021  Volume 2021

    Abstract: Introduction. Cholestasis is caused by malfunction of the hepatobiliary system. This disorder is the result of the accumulation of bile fatty acids and other toxins in the liver. The aim of the current study was to investigate the antioxidative and ... ...

    Abstract Introduction. Cholestasis is caused by malfunction of the hepatobiliary system. This disorder is the result of the accumulation of bile fatty acids and other toxins in the liver. The aim of the current study was to investigate the antioxidative and hepatoprotective effects of methanolic extract of Origanum majorana L. (OM) on hepatic disorder and tissue damage induced by bile duct ligation (BDL) in rats. Materials and methods. Twenty-eight male Wistar rats were randomly divided into 4 groups including sham control group received vehicle (SC-V), bile duct ligation received vehicle (BDL-V), bile duct ligation group received OM extract (BDL + OM), and sham control group received OM extract (SC + OM). One day after surgery, the animals received vehicle or methanolic extract of OM 300 mg/kg/day for 7 consecutive days by oral gavage. Finally, the animals were anesthetized and the blood samples were collected from each animal. After sacrificing of animals, liver tissue from each rat was removed and divided into three parts: one part was used for preparing of homogenized tissue, one part was fixed in 10% neutral formalin for histopathology examination, and the third part was kept in liquid nitrogen for gene expression analysis. Biomarkers of oxidative stress in the liver tissue and serum, as well as histopathological changes of the liver, were assessed. Also, the gene expression of IL-1, TNF-α, TGF-β, and α-SMA has been measured. Results. The results showed that BDL-V significantly increased the activity of ALT, AST, ALP, and total bilirubin compared to the SC-V group. The oxidative stress markers such as MDA and FRAP significantly increased due to BDL, while the CAT activity reduced in the BDL-V group compared to SC-V group. Oral treatment with OM reduced ALT and AST activity, although it was not statistically significant. OM treatment considerably increased the activity of CAT compared to BDL group. BDL-V induced a significant histological change in the liver, while treatment with OM at a dose of 300 mg/kg showed a ...
    Keywords Other systems of medicine ; RZ201-999
    Subject code 630
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Fabrication and Characterization of Nanocomposite Hydrogel Based on Alginate/Nano-Hydroxyapatite Loaded with Linum usitatissimum Extract as a Bone Tissue Engineering Scaffold

    Mahnaz Mohammadpour / Hadi Samadian / Nader Moradi / Zhila Izadi / Mahdieh Eftekhari / Masoud Hamidi / Amin Shavandi / Anthony Quéro / Emmanuel Petit / Cédric Delattre / Redouan Elboutachfaiti

    Marine Drugs, Vol 20, Iss 20, p

    2022  Volume 20

    Abstract: In the current paper, we fabricated, characterized, and applied nanocomposite hydrogel based on alginate (Alg) and nano-hydroxyapatite (nHA) loaded with phenolic purified extracts from the aerial part of Linum usitatissimum (LOH) as the bone tissue ... ...

    Abstract In the current paper, we fabricated, characterized, and applied nanocomposite hydrogel based on alginate (Alg) and nano-hydroxyapatite (nHA) loaded with phenolic purified extracts from the aerial part of Linum usitatissimum (LOH) as the bone tissue engineering scaffold. nHA was synthesized based on the wet chemical technique/precipitation reaction and incorporated into Alg hydrogel as the filler via physical cross-linking. The characterizations (SEM, DLS, and Zeta potential) revealed that the synthesized nHA possess a plate-like shape with nanometric dimensions. The fabricated nanocomposite has a porous architecture with interconnected pores. The average pore size was in the range of 100–200 µm and the porosity range of 80–90%. The LOH release measurement showed that about 90% of the loaded drug was released within 12 h followed by a sustained release over 48 h. The in vitro assessments showed that the nanocomposite possesses significant antioxidant activity promoting bone regeneration. The hemolysis induction measurement showed that the nanocomposites were hemocompatible with negligible hemolysis induction. The cell viability/proliferation confirmed the biocompatibility of the nanocomposites, which induced proliferative effects in a dose-dependent manner. This study revealed the fabricated nanocomposites are bioactive and osteoactive applicable for bone tissue engineering applications.
    Keywords tissue engineering ; alginate hydrogel ; nanocomposite ; nano-hydroxyapatite ; Linum usitatissimum phenolics extract ; Biology (General) ; QH301-705.5
    Subject code 620
    Language English
    Publishing date 2022-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Evaluation of Asarum europaeum L. Rhizome for the Biological Activities Related to Alzheimer’s Disease

    Mina Saeedi / Yasaman Vahedi-Mazdabadi / Arezoo Rastegari / Mahdieh Soleimani / Mahdieh Eftekhari / Tahmineh Akbarzadeh / Mahnaz Khanavi*

    Research Journal of Pharmacognosy, Vol 7, Iss 3, Pp 25-

    2020  Volume 33

    Abstract: Background and objectives: Asarum europaeum L. is an herbal medicine belonging to the family Aristolochiaceae. The rhizome of the plant has been used for the treatment of various diseases in complementary and alternative medicine of various countries. In ...

    Abstract Background and objectives: Asarum europaeum L. is an herbal medicine belonging to the family Aristolochiaceae. The rhizome of the plant has been used for the treatment of various diseases in complementary and alternative medicine of various countries. In Iranian traditional medicine (ITM), the aqueous extract of the rhizome has been used for the improvement and enhancement of memory. Methods: In the present study, the aqueous and hydroalcoholic extracts as well as different fractions of A. europaeum rhizome were evaluated for their cholinesterase (ChE), acetyl- and butyrylcholinesterase (AChE and BChE) inhibitory activity viamodified Ellman's method. Results: The ethyl acetate fraction selectively showed the most suitable anti-AChE activity (IC 50 = 99.69 µg/mL); none of the extracts or fractions demonstrated anti-BChE activity. In this regard, the ethyl acetate fraction was candidate for the investigation of further biological activities such as antioxidant activity, neuroprotectivity, and metal chelating ability related to Alzheimer’s disease. It depicted favorable neuroprotectivity at concentration of 100 μg/mL against the toxicity of exposure to H 2 O 2 in PC12 cells (p£0.001, cell viability = 80/60%) and chelating ability towards zinc, iron, and copper ions. The results of antioxidant activity by DPPH assay showed that the ethyl acetate fraction was much more potent than BHA as the reference drug. Conclusion: The ethyl acetate fraction of A. europaeum L. showed potent biological activities involved in Alzheimer’s disease and needs complementary investigations to develop an herbal product against Alzheimer’s disease.
    Keywords alzheimer’s disease ; asarum europaeum ; cholinesterase inhibitors ; neuroprotection ; traditional medicine ; Pharmacy and materia medica ; RS1-441
    Subject code 500
    Language English
    Publishing date 2020-07-01T00:00:00Z
    Publisher Iranian Society of Pharmacognosy
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Portulaca oleracea methanolic extract attenuate bile duct ligation-induced acute liver injury through hepatoprotective and anti-inflammatory effects

    Zahra Moslemi / Mina Bahrami / Ebrahim Hosseini / Mahboubeh Mansourian / Zahra Daneshyar / Mahdieh Eftekhari / Nasrin Shakerinasab / Arash Asfaram / Esmaeel Panahi kokhdan / Zahra Barmoudeh / Amir Hossein Doustimotlagh

    Heliyon, Vol 7, Iss 7, Pp e07604- (2021)

    2021  

    Abstract: Introduction: Cholestasis is a liver disease caused by a malfunction of the hepato-biliary system. Oxidative stress as a systemic complication is the main characteristic of cholestasis. The aim of this study was to evaluate the anti-inflammatory and ... ...

    Abstract Introduction: Cholestasis is a liver disease caused by a malfunction of the hepato-biliary system. Oxidative stress as a systemic complication is the main characteristic of cholestasis. The aim of this study was to evaluate the anti-inflammatory and hepatoprotective effects of Portulaca oleracea (PO) methanolic extract on liver dysfunction and tissue damage induced by bile duct ligation (BDL) in rats. Materials and methods: Twenty-eight male Wistar rats were randomly divided into four groups: sham control (SC), BDL alone, SC plus 500 mg/kg methanolic extract of PO orally for 1 week, and BDL plus 500 mg/kg methanolic extract of PO orally for 1 week. After 1 week, the animals were anesthetized, and the liver and blood samples were taken from each animal. Biochemical parameters, oxidative stress biomarkers, histopathological changes, as well as the gene expression of IL-1, TNF-α, TGF-β, and α-SMA have been evaluated. Results: The methanolic extract of PO at a dose of 500 mg/kg significantly decreased the plasma levels of aminotransferases, alkaline phosphatase as compared to BDL group (P < 0.05), while it had no significant effect on the levels of oxidative stress markers in the hepatic tissue. The plasma level of malondialdehyde and ferric-reducing antioxidant power were markedly elevated in the BDL group in comparison to SC group (P < 0.05), while treatment with PO significantly reduced these markers (P < 0.05). The administration of PO attenuated hydroxyproline content, bile duct proliferation, and inflammation score in the cholestatic liver in contrast to non-treated BDL rats (P < 0.05). Moreover, the methanolic extract of PO markedly declined the expression of TNF-α and TGF-β pro inflammatory genes in contrast to BDL rats. Conclusions: Taken together, our findings showed that PO attenuated liver injury by decreasing liver function tests, inflammation, and hydroxyproline content. As a result, it is suggested that PO can be applied in cholestatic liver damage as a therapeutic or adjuvant agent.
    Keywords Cholestasis ; Portulaca oleracea ; Oxidative stress ; Anti-inflammatory agents ; Bile ducts ; Rats ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
    Subject code 610
    Language English
    Publishing date 2021-07-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Cytotoxic Potential of Centaurea bruguierana ssp. belangerana

    Seyed Nasser Ostad / Afsaneh Rajabi / Rahim Khademi / Fatemeh Farjadmand / Mahdieh Eftekhari / Abbas Hadjiakhoondi / Mahnaz Khanavi

    Acta Medica Iranica, Vol 54, Iss

    The MTT Assay

    2016  Volume 9

    Abstract: The genus Centaurea with an extensive background in Iranian traditional medicine represents more than 70 species in Iran that some of them are endemic to Iran. A variety of secondary metabolites has been isolated from this genus thus far. Sesquiterpene ... ...

    Abstract The genus Centaurea with an extensive background in Iranian traditional medicine represents more than 70 species in Iran that some of them are endemic to Iran. A variety of secondary metabolites has been isolated from this genus thus far. Sesquiterpene lactones and flavonoids have been reported as main compounds of C.bruguierana. Methanolic extract and different fractions of the whole fruiting samples of Centaurea bruguierana ssp.belangerana (Asteraceae) were examined for cytotoxicity against various cell lines using MTT cytotoxicity assay in order to identify active fraction(s). The chloroform and ethyl acetate fractions of plant have demonstrated significant cytotoxicity against colon adenocarcinoma and breast ductal carcinoma cell lines, which chloroform fraction, exhibited the most potent in vitro cytotoxic activity against colon adenocarcinoma cell line, and therefore, can be considered as the potential fraction through main compounds of plant against the adenocarcinoma colon cancer cell line. Moreover, only chloroform fraction was moderately active against Swiss embryo fibroblast cell lines while the other fractions were nontoxic. To summarize, the chloroform fraction of C.bruguierana demonstrated better cytotoxic activity against all of the tested cell lines compared with the other fractions; and promisingly in some cases represented moderate to very good cytotoxicity that suggests more investigations about its phytochemical properties.
    Keywords Centaurea bruguierana ssp.belangerana ; Asteraceae ; Cytotoxicity ; MTT assay ; IC50 ; Medicine (General) ; R5-920
    Subject code 500
    Language English
    Publishing date 2016-10-01T00:00:00Z
    Publisher Tehran University of Medical Sciences
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Molecularly Imprinted Polymer of Colocynthin, An Effective Tool for Quality Control of Citrullus colocynthis Extracts.

    Farid, Ramezani / Mahnaz, Khanavi / Ardekani Mohammad Reza, Shams / Börje, Sellergren / Mahdieh, Eftekhari / Hossein, Rastegar / Maryam, Shekarchi

    Current drug discovery technologies

    2017  Volume 14, Issue 3, Page(s) 169–180

    Abstract: Background: Different parts of Colocynth, Citrullus colocynthis (L.) Schrad., are used in traditional phytotherapy and homeopathy.: Objective: In our new approach, a molecularly imprinted polymer was synthesized to absorb colocynthin, the major plant ...

    Abstract Background: Different parts of Colocynth, Citrullus colocynthis (L.) Schrad., are used in traditional phytotherapy and homeopathy.
    Objective: In our new approach, a molecularly imprinted polymer was synthesized to absorb colocynthin, the major plant marker, and its capability was evaluated using HPLC-UV.
    Method: A new method was considered to achieve optimal conditions. FT-infrared, N2 adsorption porosimetry, fluorescent and scanning electron microscopy and thermo gravimetric profile of the polymers were studied. The imprinted polymer was applied as molecularly imprinted solid phase extraction sorbent to enrich colocynthin from colocynth oil extract, a traditional medicine dosage form.
    Results: The imprinted polymer showed high capacity and affinity toward colocynthin. Physical assessments demonstrated no major differences between imprinted and nonimprinted polymers. The imprinted polymer was able to absorb colocynthin more efficiently than non-imprinted and control simple solvent extraction from the real sample.
    Conclusion: In conclusion, this polymer is capable of being applied as a promising adsorbent for analysis of colocynth traditional medicine products.
    Language English
    Publishing date 2017
    Publishing country United Arab Emirates
    Document type Journal Article
    ISSN 1875-6220
    ISSN (online) 1875-6220
    DOI 10.2174/1570163814666170104152226
    Database MEDical Literature Analysis and Retrieval System OnLINE

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