Article ; Online: CircMiMi: a stand-alone software for constructing circular RNA-microRNA-mRNA interactions across species.
2022 Volume 23, Issue 1, Page(s) 164
Abstract: Background: Circular RNAs (circRNAs) are a class of non-coding RNAs formed by pre-mRNA back-splicing, which are widely expressed in animal/plant cells and often play an important role in regulating microRNA (miRNA) activities. While numerous databases ... ...
Abstract | Background: Circular RNAs (circRNAs) are a class of non-coding RNAs formed by pre-mRNA back-splicing, which are widely expressed in animal/plant cells and often play an important role in regulating microRNA (miRNA) activities. While numerous databases have collected a large amount of predicted circRNA candidates and provided the corresponding circRNA-regulated interactions, a stand-alone package for constructing circRNA-miRNA-mRNA interactions based on user-identified circRNAs across species is lacking. Results: We present CircMiMi (circRNA-miRNA-mRNA interactions), a modular, Python-based software to identify circRNA-miRNA-mRNA interactions across 18 species (including 16 animals and 2 plants) with the given coordinates of circRNA junctions. The CircMiMi-constructed circRNA-miRNA-mRNA interactions are derived from circRNA-miRNA and miRNA-mRNA axes with the support of computational predictions and/or experimental data. CircMiMi also allows users to examine alignment ambiguity of back-splice junctions for checking circRNA reliability and examine reverse complementary sequences residing in the sequences flanking the circularized exons for investigating circRNA formation. We further employ CircMiMi to identify circRNA-miRNA-mRNA interactions based on the circRNAs collected in NeuroCirc, a large-scale database of circRNAs in the human brain. We construct circRNA-miRNA-mRNA interactions comprising differentially expressed circRNAs, and miRNAs in autism spectrum disorder (ASD) and cross-species analyze the relevance of the targets to ASD. We thus provide a rich set of ASD-associated circRNA-miRNA-mRNA axes and a useful starting point for investigation of regulatory mechanisms in ASD pathophysiology. Conclusions: CircMiMi allows users to identify circRNA-mediated interactions in multiple species, shedding light on regulatory roles of circRNAs. The software package and web interface are freely available at https://github.com/TreesLab/CircMiMi and http://circmimi.genomics.sinica.edu.tw/ , respectively. |
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MeSH term(s) | Animals ; Autism Spectrum Disorder ; Gene Regulatory Networks ; MicroRNAs/genetics ; RNA, Circular ; RNA, Messenger/genetics ; Reproducibility of Results ; Software |
Chemical Substances | MicroRNAs ; RNA, Circular ; RNA, Messenger |
Language | English |
Publishing date | 2022-05-06 |
Publishing country | England |
Document type | Journal Article |
ZDB-ID | 2041484-5 |
ISSN | 1471-2105 ; 1471-2105 |
ISSN (online) | 1471-2105 |
ISSN | 1471-2105 |
DOI | 10.1186/s12859-022-04692-0 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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