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Article: A rare case of persistent postendodontic symptomatic maxillary central incisor with aberrant canal configuration confirmed by cone-beam computer tomography and its nonsurgical management by retreatment.

Bhattacharyya, Anirban / Majumder, Gayatri / Maity, Asim Bikash / Datta, Soham

2023 Volume 26, Issue 3, Page(s) 359–363

Abstract: Maxillary central incisor is considered the tooth with least anatomical variations. In literature, the prevalence of single root and single canal in maxillary central incisor has been reported as 100%. Only a handful of case reports suggesting more than ... ...

Abstract	Maxillary central incisor is considered the tooth with least anatomical variations. In literature, the prevalence of single root and single canal in maxillary central incisor has been reported as 100%. Only a handful of case reports suggesting more than one root or one canal are available and are mostly associated with developmental anomalies such as gemination and fusion. This article describes a rare case report of retreatment of a maxillary central incisor with two roots with normal clinical crown which was confirmed by cone-beam computer tomography (CBCT). A 50-year-old Indian male patient presented with pain and discomfort on a root canal-treated anterior tooth. Pulp sensibility testing of left maxillary central incisor was negative. Intraoral periapical digital radiograph revealed an obturated canal with suspected outline of a second root which got confirmed with cone shift technique. The tooth was treated under dental operating microscope during which two canals were located and retreatment was completed. Postobturation, CBCT was performed to study the root and canal morphology. Clinically and radiographically, all the follow-up examinations revealed an asymptomatic tooth without any active periapical lesion. The present case report emphasizes the fact that clinicians should approach each case with an open mind having a thorough knowledge of the normal tooth anatomy and should suspect variations in every case to ensure successful endodontic outcome.
Language	English
Publishing date	2023-05-16
Publishing country	India
Document type	Case Reports
ZDB-ID	2064481-4
ISSN	0972-0707
ISSN	0972-0707
DOI	10.4103/jcd.jcd_64_23
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Zs.A 5545: Show issues

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Book ; Online: Measurement of azimuthal dependent muon flux by 2\,m\,$\times$\,2\,m RPC stack at IICHEP-Madurai

Pethuraj, S. / Majumder, G. / Datar, V. M. / Mondal, N. K. / Ravindran, K. C. / Satyanarayana, B.

2019

Abstract: The proposed 50 \,kton\, INO-ICAL experiment is an upcoming underground high energy physics experiment planned to be commissioned at Bodi hills near Theni, India ($9^{\circ}57'N$, $77^{\circ}16'E$) to study various properties of neutrino oscillations ... ...

Abstract	The proposed 50 \,kton\, INO-ICAL experiment is an upcoming underground high energy physics experiment planned to be commissioned at Bodi hills near Theni, India ($9^{\circ}57'N$, $77^{\circ}16'E$) to study various properties of neutrino oscillations using atmospheric neutrinos produced by extensive air shower phenomenon. The resistive plate chamber has been chosen as the active detector element for the proposed INO-ICAL. An experimental setup consisting a stack of 12 layers of glass resistive plate chambers each with a size of $\sim$2\,m$\times$2\,m has been built at IICHEP, Madurai to study the performance and long-term stability of the resistive plate chambers(RPCs) commercially produced in large quantities by the Indian industries as well as its electronics for the front-end and subsequent signal processing. In this study, the azimuthal dependence of muon flux at various zenith angles at Madurai (9$^{\circ}$56'N, 78$^{\circ}$00'E and at an altitude of 160\,m above mean sea level) has been presented along with the comparison of Monte Carlo from CORSIKA and HONDA predictions. Comment: arXiv admin note: text overlap with arXiv:1706.00901
Keywords	High Energy Physics - Experiment ; Physics - Instrumentation and Detectors
Subject code	621
Publishing date	2019-05-01
Publishing country	us
Document type	Book ; Online
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Article: Assessment of noise environment during construction of a major bridge and associated approach road.

Roy, T K / Mukhopadhyay, A R / Ghosh, S K / Majumder, G

Journal of environmental science & engineering

2011 Volume 53, Issue 4, Page(s) 483–486

Abstract: In this paper a methodology to quantify the noise environment, during a major bridge construction and upgrading approach road connectivity, has been provided. Noise levels were monitored at eleven sites. These eleven sites have been classified into three ...

Abstract	In this paper a methodology to quantify the noise environment, during a major bridge construction and upgrading approach road connectivity, has been provided. Noise levels were monitored at eleven sites. These eleven sites have been classified into three categories - commercial, residential and silence zones. The study was carried out to measure the ambient noise levels in all the eleven sites falling in the above three categories during both day and night times considering both "working" and "non-working" days. It was found that the mean noise level during night time was more, compared to that during day time for commercial, residential as well as silence zones. The likely causes of more noise during night time have been explored. Appropriate remedial measures have been suggested to reduce the noise levels. In addition, the noise levels in the above three zones have been compared, wherever feasible statistically, with the respective zonal standards. Significance has been found in all the cases. The underlying causes and remedies have been provided.
MeSH term(s)	Environmental Monitoring/instrumentation ; Environmental Monitoring/methods ; Equipment Design ; Noise ; Sound Spectrography/instrumentation ; Sound Spectrography/methods ; Transportation
Language	English
Publishing date	2011-10
Publishing country	India
Document type	Journal Article
ZDB-ID	2161116-6
ISSN	0367-827X
ISSN	0367-827X
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Zs.B 1702: Show issues

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Book ; Online: Design, fabrication and large scale qualification of cosmic muon veto scintillator detectors

Saraf, Mandar / Chinnappan, Pandi Raj / Deodhar, Aditya / Jangra, Mamta / Krishnamoorthi, J. / Majumder, Gobinda / Padmavathy, Veera / Ravindran, K. C. / Shah, Raj Bhupen / Shinde, Ravindra / Satyanarayana, B.

2023

Abstract: The INO collaboration is designing a cosmic muon veto detector (CMVD) to cover the mini-ICAL detector which is operational at the IICHEP transit campus, Madurai in South India. The aim of the CMVD is to study the feasibility of building an experiment to ... ...

Abstract	The INO collaboration is designing a cosmic muon veto detector (CMVD) to cover the mini-ICAL detector which is operational at the IICHEP transit campus, Madurai in South India. The aim of the CMVD is to study the feasibility of building an experiment to record rare events at a shallow depth of around 100 m, and use plastic scintillators to veto atmospheric muons from those produced by the rare interactions within the target mass of the detector. The efficiency of such a veto detector should be better than 99.99% and false positive rate of less than $10^{-5}$. The CMVD is being built using extruded plastic scintillator (EPS) strips to detect and tag atmospheric muons. More than 700 EPS strips are required to build the CMVD. Two EPS strips are pasted together to make a di-counter (DC) and wavelength shifting fibres are embedded inside the EPS strips to trap the scintillation light generated by a passing cosmic ray muon and transmit it as secondary photons to the Silicon Photo-Multipliers (SiPMs) mounted at the two ends of the DCs. Since the efficiency requirement of the veto detector is rather high, it is imperative to thoroughly test each and every component used for building the CMVD. A cosmic ray muon telescope has been setup using the DCs to qualify all the DCs that will be fabricated. In this paper we will discuss the details of the design and fabrication of the DCs, and the cosmic muon setup and the electronics used for their testing and the test results.
Keywords	Physics - Instrumentation and Detectors ; High Energy Physics - Experiment
Subject code	621
Publishing date	2023-01-29
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book ; Online: Leak Test of Resistive Plate Chamber Gap by Monitoring Absolute Pressure

Mondal, Suryanarayan / Datar, V. M. / Majumder, Gobinda / Mondal, N. K. / Ravindran, K. C. / Satyanarayana, B.

2018

Abstract: The India-based Neutrino Observatory Project (INO) is a proposed underground high energy physics experiment at Theni, India to study the neutrino oscillation parameters using atmospheric neutrinos. The 50 kton magnetised INO-ICAL detector will require ... ...

Abstract	The India-based Neutrino Observatory Project (INO) is a proposed underground high energy physics experiment at Theni, India to study the neutrino oscillation parameters using atmospheric neutrinos. The 50 kton magnetised INO-ICAL detector will require approximately 30,000 of 2m$\times$2m Resistive Plate Chambers (RPC) as sensitive detectors and proposed to operate for about 20 years. For success of the experiment, each of the RPCs has to function without showing any significant aging during the period of operation. Hence, various tests including a proper leak test are performed during and after production. The methods of leak rate calculation using conventional manometer are valid only when both the volume of the test subject and ambient pressure are kept constant. But both these quantities for a RPC gas gap depend widely on the ambient pressure and temperature. A proper quantitative estimation of the leak rate cannot be acquired from such pressure measurements. By monitoring the absolute pressures, both outside and inside of an RPC, along with the temperature, its leakage rate can be estimated. During the test period, the supporting button spacers inside an RPC may get detached due to manufacturing defect. This effect also needs to be detected. Comment: 15 pages
Keywords	Physics - Instrumentation and Detectors ; High Energy Physics - Experiment
Subject code	621
Publishing date	2018-12-01
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book ; Online: Qualification study of SiPMs on a large scale for the CMVD Experiment

Jangra, Mamta / Bhupen, Raj / Majumder, Gobinda / Gothe, Kiran / Saraf, Mandar / Parmar, Nandkishor / Satyanarayana, B. / Shinde, R. R. / Rao, Shobha K. / Upadhya, Suresh S / Datar, Vivek M / Glenzinski, Douglas A. / Bross, Alan / Pla-Dalmau, Anna / Zutshi, Vishnu V. / Group, Robert Craig / Dukes, E Craig

2022

Abstract: A Cosmic Muon Veto (CMV) detector using extruded plastic scintillators is being designed around the mini-Iron Calorimeter (mini-ICAL) detector at the transit campus of the India based Neutrino Observatory, Madurai for the feasibility study of shallow ... ...

Abstract	A Cosmic Muon Veto (CMV) detector using extruded plastic scintillators is being designed around the mini-Iron Calorimeter (mini-ICAL) detector at the transit campus of the India based Neutrino Observatory, Madurai for the feasibility study of shallow depth underground experiments. The scintillation signals that are produced in the plastic due to muon trajectories are absorbed by wavelength shifting (WLS) fibres. The WLS fibres re-emit photons of longer wavelengths and propagate those to silicon photo-multipliers (SiPMs). The SiPMs detect these photons, producing electronic signals. The CMV detector will use more than 700 scintillators to cover the mini-ICAL detector and will require around 3000 SiPMs. The design goal for the cosmic muon veto efficiency of the CMV is >99.99%. Hence, every SiPM used in the detector needs to be tested and characterised to satisfy the design goal of CMV. A mass testing system was developed for the measurement of gain and choice of the overvoltage ($V_{ov}$) of each SiPMs using an LED driver. The $V_{ov}$ is obtained by studying the noise rate, the gain of the SiPM. This paper describes the experimental setup used to test the SiPMs characteristics along with detailed studies of those characteristics as a function of temperature. Comment: 16 pages, 20 figures
Keywords	Physics - Instrumentation and Detectors ; High Energy Physics - Experiment
Subject code	621
Publishing date	2022-03-31
Publishing country	us
Document type	Book ; Online
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Article: Isolation and identification of a novel motility-inhibiting factor from goat cauda sperm plasma membrane.

Dungdung, S R / Majumder, G C

Cellular and molecular biology (Noisy-le-Grand, France)

2003 Volume 49, Issue 3, Page(s) 413–420

Abstract: A motility inhibiting factor (MIF) in sperm plasma membrane of mammalian spermatozoa (goat) has been demonstrated. This factor has been purified to apparent homogeneity by Sepharose-6B affinity chromatography and DEAE-cellulose ion-exchange ... ...

Abstract	A motility inhibiting factor (MIF) in sperm plasma membrane of mammalian spermatozoa (goat) has been demonstrated. This factor has been purified to apparent homogeneity by Sepharose-6B affinity chromatography and DEAE-cellulose ion-exchange chromatography. The molecular weight of the isolated factor has been estimated as 98 kDa by molecular sieving and analytical HPLC. SDS-polyacrylamide gel electrophoresis of MIF gave a single band of 100 kDa, indicating that the factor is a monomer. MIF is a thermo-stable factor and it inhibited the spermatozoa motility in a dose dependent manner. It is a glycoprotein as it binds with high affinity to Sepharose-6B and the affinity matrix-bound factor can be eluted with D-galactose. Data show that the motility inhibiting activity is lost completely when treated with beta-galactosidase indicating that its sugar side chain is essential for its activity. Addition of MIF antibody caused significant enhancement of forward motility of the caput and cauda-spermatoza. This antibody may thus be useful for solving some of the problems of human infertility due to low sperm motility. The motility inhibiting protein may also be useful as a vaginal contraceptive.
MeSH term(s)	Animals ; Cell Membrane/chemistry ; Chromatography, DEAE-Cellulose ; Electrophoresis, Polyacrylamide Gel ; Epididymis ; Goats ; Humans ; Male ; Membrane Glycoproteins/chemistry ; Membrane Glycoproteins/isolation & purification ; Membrane Glycoproteins/pharmacology ; Membrane Glycoproteins/physiology ; Semen/chemistry ; Sperm Motility/drug effects ; Spermatozoa/chemistry ; Spermatozoa/drug effects ; Spermatozoa/physiology
Chemical Substances	Membrane Glycoproteins
Language	English
Publishing date	2003-05
Publishing country	France
Document type	Journal Article
ZDB-ID	1161779-2
ISSN	0145-5680
ISSN	0145-5680
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Zs.A 3812: Show issues

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Article ; Online: Role of the major ecto-phosphoprotein in sperm flagellar motility using a cell electroporation method.

Maiti, Arunima / Mishra, K P / Majumder, G C

Molecular reproduction and development

2008 Volume 75, Issue 7, Page(s) 1185–1195

Abstract: Previous studies from our laboratory have identified MPS, a 100-kDa protein, as the major phosphoprotein substrate of caprine sperm ecto-cyclic AMP independent protein kinase. In this study the isolated (32)P-labelled MPS has been incorporated into ... ...

Abstract	Previous studies from our laboratory have identified MPS, a 100-kDa protein, as the major phosphoprotein substrate of caprine sperm ecto-cyclic AMP independent protein kinase. In this study the isolated (32)P-labelled MPS has been incorporated into mature caprine (Capra indicus) cauda-epididymal spermatozoa with the help of cell electroporation technique to investigate the effect of MPS on sperm flagellar motility. The optimum conditions for electroporation of sperm cells consisted of exposure of 0.2 ml of sperm cells (2 x 10(8)/ml) to external electric field of intensity 1.5 kV/cm and capacitation of 25 microF at 4 degrees C and post-pulse incubation at 37 degrees C for 1 hr. when nearly 50% of the cells lost motility. Scanning electron micrographs (SEM) demonstrate the formation of micro-pores and local osmotic swelling in the electroporated spermatozoa. MPS incorporation was maximal when its concentration was 30 microg/ml (300 pmol) in the medium and when the post-pulse incubation time was 60 min. At maximum (75%) MPS incorporation, total and forward motility increments were also maximum: 34% (P < 0.01) and 32% (P < 0.01), respectively. The subcellular fractionation data show that major portion of the introduced MPS was bound to the plasma-membrane of spermatozoa. The 32P-labelled electrophoresed intact spermatozoa lost radioactivity due to the action of the endogenous ecto-phosphoprotein phosphatase. Therefore MPS is primarily localised on the sperm external surface leaving its phosphate group(s) oriented in the extracellular medium. The data provided further evidence to strengthen the view that MPS is an ecto-phosphoprotein and that it plays an important role in the regulation of sperm flagellar motility.
MeSH term(s)	Animals ; Cell Membrane/physiology ; Cell Movement ; Electric Stimulation ; Electroporation/methods ; Epididymis ; Flagella/physiology ; Goats ; Kinetics ; Male ; Phosphoproteins/physiology ; Phosphorylation ; Sperm Motility/physiology ; Spermatozoa/physiology
Chemical Substances	Phosphoproteins
Language	English
Publishing date	2008-07
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	20321-x
ISSN	1098-2795 ; 1040-452X
ISSN (online)	1098-2795
ISSN	1040-452X
DOI	10.1002/mrd.20854
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Zs.A 2759: Show issues

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Article ; Online: In vitro initiated sperm forward motility in caput spermatozoa: weak and transient.

Das, K / Das, S / Bhoumik, A / Jaiswal, B S / Majumder, G C / Dungdung, S R

Andrologia

2012 Volume 44 Suppl 1, Page(s) 807–812

Abstract: Testicular spermatozoa during journey through epididymis acquire forward motility, which is essential for fertility. To understand the biochemistry of sperm motility initiation, various initiation media have been developed that permitted high level of ... ...

Abstract	Testicular spermatozoa during journey through epididymis acquire forward motility, which is essential for fertility. To understand the biochemistry of sperm motility initiation, various initiation media have been developed that permitted high level of motility induction (55-60%) in the immature caput-spermatozoa in presence of activating principles: theophylline, bicarbonate and epididymal plasma (EP) when analysed microscopically. Here, we show for the first time using caprine model that stability and quality of in vitro-induced motility in the caput spermatozoa is insignificant in contrast to naturally induced motility in mature cauda spermatozoa. In vitro-induced motility of the immature spermatozoa was lost completely upon the removal of these activators by centrifugation. Selective withdrawal of either EP or HCO(3) by dilution retains 50-60% of the in vitro-induced motility. Spectrophotometric analysis revealed that in vitro-induced vertical motility in immature spermatozoa is too little when compared to mature spermatozoa. In in vitro-initiated caput spermatozoa, cyclic adenosine monophosphate level becomes doubled but lesser than cauda spermatozoa. This revelation concludes that scientific knowledge generated over the years on the basis of in vitro initiation method is insignificant and needs improvisation to delineate biochemical regulation of sperm motility which in turn has remarkable potential in wide biological fields, especially in infertility treatment.
MeSH term(s)	Animals ; Centrifugation ; Cyclic AMP/metabolism ; Goats ; In Vitro Techniques ; Male ; Sperm Motility ; Spermatozoa/metabolism
Chemical Substances	Cyclic AMP (E0399OZS9N)
Language	English
Publishing date	2012-05
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	7280-1
ISSN	1439-0272 ; 0303-4569
ISSN (online)	1439-0272
ISSN	0303-4569
DOI	10.1111/j.1439-0272.2011.01161.x
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Zs.A 665: Show issues

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Article: Maturation-dependent modification of the protein phosphorylation profile of isolated goat sperm plasma membrane.

Nath, D / Majumder, G C

Journal of reproduction and fertility

1999 Volume 115, Issue 1, Page(s) 29–37

Abstract: Highly purified plasma membranes, isolated by an aqueous two-phase polymer method from goat epididymal spermatozoa, were found to possess a kinase activity that causes phosphorylation of serine and threonine residues of several endogenous plasma membrane ...

Abstract	Highly purified plasma membranes, isolated by an aqueous two-phase polymer method from goat epididymal spermatozoa, were found to possess a kinase activity that causes phosphorylation of serine and threonine residues of several endogenous plasma membrane proteins. Cyclic AMP, cyclic GMP, Ca(2+)-calmodulin, phosphatidylserine-diolein, polyamines and heparin had no appreciable effect on this kinase. Autoradiographic analysis showed that the profile of the phosphorylation of membrane proteins by this endogenous cAMP-independent protein kinase underwent marked modulation during the transit of spermatozoa through the epididymis. In caput sperm plasma membrane, 18, 21, 43, 52, 74 and 90 kDa proteins were phosphorylated, whereas, in the corpus and cauda epididymal spermatozoa, a differential phosphorylation pattern was observed with respect to the 90, 74, 21 and 18 kDa proteins. The rate of phosphorylation of the 74 kDa protein decreased markedly during the early phase of sperm maturation (caput to distal corpus epididymides) whereas there was little change in kinase activity in sperm plasma membrane. In contrast, the rates of phosphorylation of the 18 and 21 kDa proteins increased during the terminal phase (distal corpus to distal cauda epididymides) of sperm maturity, although the kinase activity of membrane decreased significantly during this phase. The modulation of the phosphorylated states of these specific membrane proteins may play an important role in the maturation of epididymal spermatozoa.
MeSH term(s)	Adenosine Triphosphate/metabolism ; Adenosine Triphosphate/pharmacology ; Animals ; Autoradiography ; Cell Membrane/chemistry ; Cell Membrane/metabolism ; Epididymis ; Goats/metabolism ; Male ; Membrane Proteins/analysis ; Membrane Proteins/metabolism ; Phosphoproteins/analysis ; Phosphoproteins/metabolism ; Phosphorylation ; Protein Kinases/metabolism ; Sperm Maturation/physiology ; Spermatozoa/metabolism
Chemical Substances	Membrane Proteins ; Phosphoproteins ; Adenosine Triphosphate (8L70Q75FXE) ; Protein Kinases (EC 2.7.-)
Language	English
Publishing date	1999-01
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80167-7
ISSN	0022-4251
ISSN	0022-4251
DOI	10.1530/jrf.0.1150029
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