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Article ; Online: Evaluation of Disinfection Methods for Artificial Plants in Zebrafish (

Camson, Christina T / Palillo, Jack A / Fehrenbach, Logan A / Malbrue, Raphael A

Journal of the American Association for Laboratory Animal Science : JAALAS

2023 Volume 62, Issue 6, Page(s) 512–517

Abstract: The use of artificial plants as environmental enrichment for zebrafish ( ...

Abstract	The use of artificial plants as environmental enrichment for zebrafish (
MeSH term(s)	Animals ; Disinfection/methods ; Water ; Zebrafish ; Animal Welfare ; Adenosine Triphosphate
Chemical Substances	Water (059QF0KO0R) ; Adenosine Triphosphate (8L70Q75FXE)
Language	English
Publishing date	2023-11-30
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2769-6677
ISSN (online)	2769-6677
DOI	10.30802/AALAS-JAALAS-23-000042
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Scrotal ablation and orchiectomy in the domestic laboratory goat (Capra hircus)

Malbrue, Raphael A / Arsuaga Zorilla, Carmen B

Veterinary and Animal Science. 2018 June, v. 5

2018

Abstract: Domestic goats (Capra hircus) have been used as animal models in biomedical research, and for numerous years for agricultural research and models for human diseases. This column describes an improved surgical technique, for castration of male goats, ... ...

Abstract	Domestic goats (Capra hircus) have been used as animal models in biomedical research, and for numerous years for agricultural research and models for human diseases. This column describes an improved surgical technique, for castration of male goats, similar to companion animal techniques for use in the laboratory setting. The technique discussed supports a more in-depth perioperative protocol for performing scrotal ablation and orchiectomy in the male goat.
Keywords	Capra hircus ; animal models ; biomedical research ; castration ; goats ; human diseases ; males ; pets
Language	English
Dates of publication	2018-06
Size	p. 26-30.
Publishing place	Elsevier Ltd
Document type	Article
ISSN	2451-943X
DOI	10.1016/j.vas.2018.02.002
Database	NAL-Catalogue (AGRICOLA)

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Article: Scrotal ablation and orchiectomy in the domestic laboratory goat (

Malbrue, Raphael A / Arsuaga Zorilla, Carmen B

Veterinary and animal science

2018 Volume 5, Page(s) 26–30

Abstract: Domestic goats ( ...

Abstract	Domestic goats (
Language	English
Publishing date	2018-02-08
Publishing country	United States
Document type	Journal Article
ISSN	2451-943X
ISSN	2451-943X
DOI	10.1016/j.vas.2018.02.002
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Internal Temperatures of Packaging for Overnight Cross-country Shipping of Zebrafish (

Fehrenbach, Logan A / Adekanye, Adewole A / Palillo, Michael B / Bidot, Willie A / Malbrue, Raphael A / Coble, Dondrae J / Kendall, Genevieve C

Comparative medicine

2022 Volume 72, Issue 3, Page(s) 195–203

Abstract: As the use of zebrafish ( ...

Abstract	As the use of zebrafish (
MeSH term(s)	Animals ; Temperature ; Zebrafish
Language	English
Publishing date	2022-06-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2006425-1
ISSN	2769-819X ; 0023-6764 ; 1532-0820
ISSN (online)	2769-819X
ISSN	0023-6764 ; 1532-0820
DOI	10.30802/AALAS-CM-22-000053
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Article: Effects of Cling Film Draping Material on Body Temperature of Mice During Surgery.

Celeste, Natalie A / Emmer, Kathryn M / Bidot, Willie A / Perret-Gentil, Marcel I / Malbrue, Raphael A

Journal of the American Association for Laboratory Animal Science : JAALAS

2020 Volume 60, Issue 2, Page(s) 195–200

Abstract: General anesthesia induces many systemic effects, including thermoregulatory impairment and subsequent perioperative hypothermia. Due to the animals' small size, monitoring and maintaining body temperatures in laboratory rodents during anesthesia is ... ...

Abstract	General anesthesia induces many systemic effects, including thermoregulatory impairment and subsequent perioperative hypothermia. Due to the animals' small size, monitoring and maintaining body temperatures in laboratory rodents during anesthesia is important for successful surgical outcomes and prompt anesthetic recovery. Draping materials have the potential to aid in thermal support during surgical anesthesia. In this study, rectal and surface (infrared) temperatures were measured in C57BL/6 mice under isoflurane anesthesia every 5 min for the duration of a 35-min sham surgery. In addition to placement on a circulating water bath, mice (
MeSH term(s)	Anesthesia, General/veterinary ; Animals ; Body Temperature ; Humans ; Male ; Mice ; Mice, Inbred C57BL ; Monitoring, Physiologic/veterinary ; Surgical Equipment
Language	English
Publishing date	2020-12-28
Publishing country	United States
Document type	Journal Article
ISSN	1559-6109
ISSN	1559-6109
DOI	10.30802/AALAS-JAALAS-20-000089
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Evaluation of the Sterility of Reynolds Wrap Aluminum Foil for Use During Rodent Surgery.

Nolan, Katherine E / Bidot, Willie A / Perret-Gentil, Marcel I / Malbrue, Raphael A

Journal of the American Association for Laboratory Animal Science : JAALAS

2020 Volume 60, Issue 1, Page(s) 85–90

Abstract: In biomedical research, surgeons are often responsible for simultaneously conducting rodent surgical procedures, monitoring anesthesia, and adjusting nonsterile equipment. Maintaining appropriate aseptic technique can be challenging when working under ... ...

Abstract	In biomedical research, surgeons are often responsible for simultaneously conducting rodent surgical procedures, monitoring anesthesia, and adjusting nonsterile equipment. Maintaining appropriate aseptic technique can be challenging when working under these conditions. Applying a sterile barrier material such as aluminum foil to nonsterile surfaces in these circumstances offers an innovative, inexpensive option to improve asepsis. The purpose of this study was to validate the sterility of foodgrade aluminum foil for use as a sterile barrier on nonsterile equipment during rodent surgery. In this investigation, 10 boxes of aluminum foil were assessed for sterility by using ATP swabs and replicate organism detection and counting (RODAC) plates at 0, 14, and 28 d and 6 mo. At 6 mo, foil was applied to surgical equipment, and sterility was assessed by using ATP swabs and RODAC plates. Results revealed no ATP-positive results at any time point. During assessment of samples obtained directly from boxes, RODAC plates yielded minimal bacterial growth (1 cfu per plate) in 2 of the 10 boxes at initial testing and in 1 box at the day 0, day 14, and 6 mo time points. No growth was observed at day 28 (tested directly from the box) or at 6-mo apparatus testing. Our data revealed minimal bacterial growth on tested samples and support the use of Reynolds Wrap aluminum foil as a sterile barrier on nonsterile surfaces during aseptic rodent surgery.
MeSH term(s)	Aluminum ; Animals ; Asepsis ; Infertility ; Rodentia ; Surgical Equipment
Chemical Substances	Aluminum (CPD4NFA903)
Language	English
Publishing date	2020-11-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1559-6109
ISSN	1559-6109
DOI	10.30802/AALAS-JAALAS-20-000078
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: PATET ratio by Doppler echocardiography: noninvasive detection of pediatric pulmonary arterial hypertension.

Trittmann, Jennifer K / Almazroue, Hanadi / Nelin, Leif D / Shaffer, Terri A / Celestine, Charanda R / Green, Henry W / Malbrue, Raphael A

Pediatric research

2021 Volume 92, Issue 3, Page(s) 631–636

Abstract: Pulmonary artery acceleration time (PAT) and PAT: ejection time (PATET) ratio are echocardiographic measurements of pulmonary arterial hypertension (PAH). These noninvasive quantitative measurements are ideal to follow longitudinally through the clinical ...

Abstract	Pulmonary artery acceleration time (PAT) and PAT: ejection time (PATET) ratio are echocardiographic measurements of pulmonary arterial hypertension (PAH). These noninvasive quantitative measurements are ideal to follow longitudinally through the clinical course of PAH, especially as it relates to the need for and/or response to treatment. This review article focuses on the current literature of PATET measurement for infants and children as it relates to the shortening of the PATET ratio in PAH. At the same time, further development of PATET as an outcome measure for PAH in preclinical models, particularly mice, such that the field can move forward to human clinical studies that are both safe and effective. Here, we present what is known about PATET in infants and children and discuss what is known in preclinical models with particular emphasis on neonatal mouse models. In both animal models and human disease, PATET allows for longitudinal measurements in the same individual, leading to more precise determinations of disease/model progression and/or response to therapy. IMPACT: PATET ratio is a quantitative measurement by a noninvasive technique, Doppler echocardiography, providing clinicians a more precise/accurate, safe, and longitudinal assessment of pediatric PAH. We present a brief history/state of the art of PATET ratio to predict PAH in adults, children, infants, and fetuses, as well as in small animal models of PAH. In a preliminary study, PATET shortened by 18% during acute hypoxic exposure compared to pre-hypoxia. Studies are needed to establish PATET, especially in mouse models of disease, such as bronchopulmonary, as a routine measure of PAH.
MeSH term(s)	Adult ; Animals ; Child ; Echocardiography ; Echocardiography, Doppler/methods ; Humans ; Hypertension, Pulmonary/diagnostic imaging ; Infant ; Mice ; Pulmonary Arterial Hypertension ; Pulmonary Artery/diagnostic imaging
Language	English
Publishing date	2021-11-18
Publishing country	United States
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	4411-8
ISSN	1530-0447 ; 0031-3998
ISSN (online)	1530-0447
ISSN	0031-3998
DOI	10.1038/s41390-021-01840-9
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Article: Evaluation of the Sterility of Press'n Seal Cling Film for Use in Rodent Surgery.

Emmer, Kathryn M / Celeste, Natalie A / Bidot, Willie A / Perret-Gentil, Marcel I / Malbrue, Raphael A

Journal of the American Association for Laboratory Animal Science : JAALAS

2019 Volume 58, Issue 2, Page(s) 235–239

Abstract: level and improve surgical outcomes. Recently, some institutions have approved the use of Press'n Seal cling film (CF; Glad Products, Oakland, CA) as a practical, cost-effective alternative to sterile drapes for rodent surgeries. The purpose of this ... ...

Abstract	level and improve surgical outcomes. Recently, some institutions have approved the use of Press'n Seal cling film (CF; Glad Products, Oakland, CA) as a practical, cost-effective alternative to sterile drapes for rodent surgeries. The purpose of this study was to evaluate the sterility of CF by using ATP and replicate organism detection and counting (RODAC) plates. We tested 10 boxes of CF at days 0, 14, and 28 after opening the box and compared the results with traditional packaged sterile drapes. Our data indicated that CF ATP bioluminescence remained at or below 10 relative light units for 28 d after opening the box. In addition, RODAC plates had no growth for 70% of CF boxes at day 0, 100% at day 14, and 90% at day 28. The mean growth for the positive plates was 0.024 cfu/cm² sampled after contacting locations on the front and back of the CF. The results of this study support the use of CF as an acceptable alternative to traditional sterile drapes during rodent aseptic surgery.
MeSH term(s)	Animals ; Bacteria/isolation & purification ; Equipment Contamination/prevention & control ; Laboratory Animal Science ; Luminescent Measurements ; Rodentia ; Stem Cells ; Surgical Equipment/microbiology ; Surgical Equipment/standards
Language	English
Publishing date	2019-02-27
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1559-6109
ISSN	1559-6109
DOI	10.30802/AALAS-JAALAS-18-000096
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Supporting diversity, equity, inclusion, and belonging to strengthen and position the veterinary profession for service, sustainability, excellence, and impact.

Burkhard, Mary Jo / Dawkins, Sandra / Knoblaugh, Sue E / El-Khoury, Caroline / Coble, Dondrae / Malbrue, Raphael A / Read, Emma K / Greenhill, Lisa M / Moore, Rustin M

Journal of the American Veterinary Medical Association

2022 Volume 260, Issue 11, Page(s) 1283–1290

Abstract: Advancing equality and equity in society is creating positive change, and the time has come to critically evaluate veterinary medicine, which, by all metrics, lacks diversity. To keep pace with increasingly diverse demographics and recent surges in pet ... ...

Abstract	Advancing equality and equity in society is creating positive change, and the time has come to critically evaluate veterinary medicine, which, by all metrics, lacks diversity. To keep pace with increasingly diverse demographics and recent surges in pet ownership among all racial/ethnic groups, significant efforts to enhance diversity, equity, inclusion, and belonging (DEIB) must occur in veterinary colleges and the profession. Recruiting more underrepresented students, building pipelines for diverse faculty/staff, and creating inclusive, welcoming environments where all can thrive are critical steps toward enhancing DEIB within our organizations and profession. Our goal is to share experiences and lessons learned from our intentional commitment to strengthen DEIB, with the hope that our journey will be helpful to others. Increasing diversity in the veterinary profession will be facilitated through removing barriers, creating inclusive work environments where all people feel they belong, and ensuring fair and equitable hiring and personnel management practices. These steps should in turn improve access and quality of veterinary care, ensure we are more representative of the communities we serve, increase revenue, and preserve the human-animal bond. "You cannot change any society unless you take responsibility for it, unless you see yourself belonging to it, and responsible for changing it." - Grace Lee Boggs.
MeSH term(s)	Animals ; Cultural Diversity ; Humans
Language	English
Publishing date	2022-05-25
Publishing country	United States
Document type	Journal Article
ZDB-ID	390811-2
ISSN	1943-569X ; 0003-1488
ISSN (online)	1943-569X
ISSN	0003-1488
DOI	10.2460/javma.21.11.0477
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Scrotal stalk ablation and orchiectomy using electrosurgery in the male sugar glider (Petaurus breviceps) and histologic anatomy of the testes and associated scrotal structures

Malbrue, Raphael A / Allen, Jannelle L / Arsuaga, Carmen B / Collins, Toi A / Diggs, Treyton J / Langohr, Ingeborg M

Journal of exotic pet medicine. 2018 Apr., v. 27, no. 2

2018

Abstract: This report describes an alternative surgical technique for performing scrotal ablation and orchiectomy in the adult male sugar glider (Petaurus breviceps) using electrosurgery. Eight animals from a local small mammal shelter group that presented for ... ...

Abstract	This report describes an alternative surgical technique for performing scrotal ablation and orchiectomy in the adult male sugar glider (Petaurus breviceps) using electrosurgery. Eight animals from a local small mammal shelter group that presented for routine surgical castration for population control measures were included in this study. Mean surgical time was 4.37 ± 2.13 seconds. Mean anesthesia time was 12.32 ± 5.63 minutes. Mean recovery time was 8.37 ± 3.68 minutes. Mean total procedure time was 22.24 ± 5.69 minutes. No perioperative complications were reported. All animals appeared to have sufficient pain management with an analgesia and anti-inflammatory protocol using butorphanol and meloxicam. No over grooming and or self-injurious behaviors were reported by the local shelter during both the 24-hour and 14-day recheck examinations. Electrosurgery scrotal ablation and orchiectomy therefore offers an alternate route for the practicing veterinarian to perform safe and efficient castrations in male sugar gliders. Histologically, in the epididymis, 3 of the 8 sugar gliders had evidence of a well-defined region in the head, between the efferent ducts and the remaining epididymis. The lining cells differed morphologically from those constituting the remaining epithelial lining. The presence of eosinophilic vacuoles in the cytoplasm of the cells in this region suggests a secretory activity.
Keywords	adults ; analgesia ; anesthesia ; butorphanol ; castration ; control methods ; epididymis ; epithelium ; head ; histology ; males ; meloxicam ; pain ; Petaurus breviceps ; small mammals ; testes ; vacuoles ; veterinarians
Language	English
Dates of publication	2018-04
Size	p. 90-94.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	2228614-7
ISSN	1557-5063
ISSN	1557-5063
DOI	10.1053/j.jepm.2018.02.037
Database	NAL-Catalogue (AGRICOLA)

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