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  1. Article ; Online: Recombinant Anti-Müllerian Hormone (rAMH) for Stalling In Vitro Granulosa Cell Replication.

    Detti, Laura / Abuzeid, Mustafa I / Peregrin-Alvarez, Irene / Christiansen, Mary E / Malekzadeh, Pouran / Sledge, Jennifer / Saed, Ghassan M

    Reproductive sciences (Thousand Oaks, Calif.)

    2020  Volume 27, Issue 10, Page(s) 1873–1878

    Abstract: To investigate whether recombinant AMH (rAMH) is able to decrease cellular proliferation/apoptosis in luteinized granulosa cells (GCs) through hormonal regulation, a primary culture of GCs was established from GCs obtained at time of oocyte retrieval ... ...

    Abstract To investigate whether recombinant AMH (rAMH) is able to decrease cellular proliferation/apoptosis in luteinized granulosa cells (GCs) through hormonal regulation, a primary culture of GCs was established from GCs obtained at time of oocyte retrieval from follicular fluid of 3 patients. Cells were seeded in well cell culture plates at a density of 100,000 cells/well in medium and treated with rAMH 20 ng/ml (rAMH group), or phosphate-buffered saline (PBS-control group), for 24 h. Total RNA was extracted from all cells, followed by cDNA synthesis and real-time RT-PCR to quantify the expression levels of AMH, AMH-R2, FSH-R, inhibin B, cell proliferation (Ki67), and apoptosis (Caspase 3). We used independent sample t test (SPSS v25) and a p < 0.05 significance. Cellular expressions of AMH, AMH-R2, FSH-R, and inhibin B were reduced greater than 50% in the rAMH group, compared with that of the the control group (p ≤ 0.005 for all). Ki67 and Caspase3 were also reduced greater than 30% in the rAMH group (p ≤ 0.001 for both). Our findings show a direct inhibitory effect of AMH on luteinized GCs' expression of the major regulatory hormones, in addition to a significant decrease in markers of cell proliferation and apoptosis. These results confirm the inhibitory effects of AMH on follicular development.
    MeSH term(s) Anti-Mullerian Hormone/metabolism ; Anti-Mullerian Hormone/pharmacology ; Apoptosis/drug effects ; Caspase 3/metabolism ; Cell Proliferation/drug effects ; Female ; Follicular Fluid ; Granulosa Cells/cytology ; Granulosa Cells/drug effects ; Humans ; Inhibins/metabolism ; Oocyte Retrieval ; Receptors, FSH/metabolism ; Receptors, Peptide/metabolism ; Receptors, Transforming Growth Factor beta/metabolism ; Recombinant Proteins/pharmacology
    Chemical Substances Receptors, FSH ; Receptors, Peptide ; Receptors, Transforming Growth Factor beta ; Recombinant Proteins ; anti-Mullerian hormone receptor ; inhibin B ; Inhibins (57285-09-3) ; Anti-Mullerian Hormone (80497-65-0) ; Caspase 3 (EC 3.4.22.-)
    Language English
    Publishing date 2020-07-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2276411-2
    ISSN 1933-7205 ; 1933-7191
    ISSN (online) 1933-7205
    ISSN 1933-7191
    DOI 10.1007/s43032-020-00206-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4113: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Vaginal Mesh Removal Outcomes: Eight Years of Experience at an Academic Hospital.

    Cardenas-Trowers, Olivia O / Malekzadeh, Pouran / Nix, David E / Hatch, Kenneth D

    Female pelvic medicine & reconstructive surgery

    2017  Volume 23, Issue 6, Page(s) 382–386

    Abstract: Objectives: The purpose of this study is to describe the clinical history leading up to and the outcomes after vaginal mesh removal surgery at an academic hospital.: Methods: A retrospective case series of patients who underwent vaginal mesh removal ... ...

    Abstract Objectives: The purpose of this study is to describe the clinical history leading up to and the outcomes after vaginal mesh removal surgery at an academic hospital.
    Methods: A retrospective case series of patients who underwent vaginal mesh removal from 2008 to 2015 was conducted. Demographics, clinical history, physical examination, pre- and postoperative symptoms, and number and type of reoperations were abstracted.
    Results: Between February 2008 and November 2015, 83 patients underwent vaginal mesh removal surgery at our hospital. The median time interval from initial mesh placement to removal was 58 months (range, 0.4-154 months). The most common preoperative symptoms were vaginal pain (n = 52, 62%), dyspareunia (n = 46, 55%), and pelvic pain (n = 42, 50%). Intraoperative complications were infrequent (n = 3, 4%). Of patients presenting for follow-up within 4 to 6 weeks postoperatively, the most common symptoms were urinary incontinence (n = 15, 28%), vaginal pain (n = 7, 13%), buttock pain (n = 5, 9%), and urinary tract infection (n = 5, 9%). There were no identifiable risk factors to predict which patients would have persistent postoperative symptoms or who would require more than 1 mesh removal surgery. After vaginal mesh removal, 29 patients (35%) required 1 or more reoperations, with 3 being the highest number of reoperations per patient. The total number of reoperations was 43, with a total of 63 individual procedures performed. Forty-four percent (n = 28) of the procedures were graft removals, 40% (n = 25) were pelvic organ prolapse surgeries (only native tissue repairs), and 16% (n = 10) were stress incontinence surgeries. More than 1 procedure was performed in 49% (n = 21) of the reoperations.
    Conclusions: Vaginal mesh removal surgery is safe; however, some patients require more than 1 procedure, and the risk factors for reoperations are unclear.
    MeSH term(s) Aged ; Female ; Hospitals, University/statistics & numerical data ; Humans ; Hysterectomy/adverse effects ; Hysterectomy/statistics & numerical data ; Middle Aged ; Pelvic Organ Prolapse/surgery ; Postoperative Complications/epidemiology ; Reoperation/statistics & numerical data ; Retrospective Studies ; Risk Factors ; Suburethral Slings/adverse effects ; Surgical Mesh/adverse effects ; Urinary Incontinence/surgery
    Language English
    Publishing date 2017-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2542707-6
    ISSN 2154-4212 ; 2151-8378
    ISSN (online) 2154-4212
    ISSN 2151-8378
    DOI 10.1097/SPV.0000000000000419
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4599: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Effect of Centruroides antivenom on reversal of methamphetamine-induced hyperkinesis and hyperthermia in rats.

    Malekzadeh, Pouran / Hu, Jackie / Sandweiss, Alexander J / Ameli, Nina / Bierny, Philippe / Largent-Milnes, Tally M / Vanderah, Todd W / Shirazi, Farshad

    Journal of pharmaceutics & pharmacology

    2017  Volume 5, Issue 1

    Abstract: Context: Methamphetamine (MA) toxicity is a major health concern causing agitation, hyperkinesia, hyperthermia, and even death, affecting 24.7 million people worldwide. It has been observed that MA generates movement disorders in children similar to ... ...

    Abstract Context: Methamphetamine (MA) toxicity is a major health concern causing agitation, hyperkinesia, hyperthermia, and even death, affecting 24.7 million people worldwide. It has been observed that MA generates movement disorders in children similar to that of scorpion envenomation. Four cases have been reported where MA intoxication in children were both subjectively and objectively improved as indicated by the reversal of nystagmus and movement disorders following administration of Centruroides antivenom (AV) therapy.
    Objective: Here, we aimed to demonstrate the reversal of MA induced movement disorders and hyperthermia by scorpion AV equine immune F(ab')2 in rats.
    Materials and methods: Baseline core temperature and locomotor activity in adult male Sprague-Dawley rats (200-220 g) were evaluated prior to acute administration of AV (20 mg/kg, intraperitoneally, i.p.) + MA (10 mg/kg, i.p.) or control. Core body temperature was reassessed 10, 50, and 80 min post injection while locomotor activity was reassessed 20-35 and 60-75 min post injection.
    Results: At 20-35 min, Saline + MA and BSA + MA groups showed a significant increase in the number of fine events compared to their respective control groups Saline + Saline and BSA + Saline, which indicates an increase in paw movements of animals
    Discussion: Here, we provide evidence for some aspects of MA-induced hyperkinesia but not hyperthermia reversed by scorpion AV. Further preclinical studies involving adolescent rodents may be necessary to completely mimic the reversal of MA toxicity seen in children in the clinic.
    Language English
    Publishing date 2017-04-20
    Publishing country United States
    Document type Journal Article
    ISSN 2327-204X
    ISSN 2327-204X
    DOI 10.13188/2327-204X.1000020
    Database MEDical Literature Analysis and Retrieval System OnLINE

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