LIVIVO - Das Suchportal für Lebenswissenschaften

switch to English language
Zurück zur letzten Suche Suchhistorie
Erweiterte Suche

Ihre letzten Suchen

  1. AU="Malik, Sajid Ali"
  2. AU="V, Gomathi"

Suchergebnis

Treffer 1 - 2 von insgesamt 2

Suchoptionen

  1. Artikel ; Online: Comparative Analysis of Physical and Chemical Mutagenesis in Chrysanthemum cv. 'Candid': Assessing Genetic Variation and Breeding Potential.

    Din, Ambreena / Qadri, Zahoor Ahmad / Wani, Muneeb Ahmad / Iqbal, Shameen / Malik, Sajid Ali / Zargar, Sajad Majeed / Banday, Neelofar / Nazki, Imtiyaz Tahir

    ACS omega

    2023  Band 8, Heft 46, Seite(n) 43836–43849

    Abstract: In this study, we developed a mutagenesis protocol specifically designed for chrysanthemum cv. "Candid" in order to introduce genetic variation. By subjecting chrysanthemum shoots to different doses of physical and chemical mutagens, we successfully ... ...

    Abstract In this study, we developed a mutagenesis protocol specifically designed for chrysanthemum cv. "Candid" in order to introduce genetic variation. By subjecting chrysanthemum shoots to different doses of physical and chemical mutagens, we successfully generated a total of 24 mutants, each with unique genetic compositions. We observed that the mortality rate was lowest when the shoots were exposed to 10 Gy gamma irradiation and 1.00% EMS. To assess the diversity and relatedness among the mutants, we employed RAPD and SSR markers. The combination of these markers allowed us to construct a dendrogram that effectively categorized the mutant population into distinct clusters based on the specific mutagen treatments. Interestingly, the mutants induced by 10 Gy gamma irradiation exhibited greater genetic diversity in terms of flower colors. On the other hand, mutants created with 1.00% EMS displayed a higher level of variation and yielded more viable mutants. To determine the optimal markers for studying genetic diversity, we analyzed the polymorphic information content (PIC) of different markers. Among the tested markers, OPA-07 (RAPD) and JH47 (SSR) showed the highest PIC values, indicating their effectiveness in capturing genetic variability within the mutant population. Conversely, the PIC values of OPD-07 and JH20 demonstrated the lowest among the markers tested. Our results revealed a percentage of polymorphism ranging from 81.81% to 100% for RAPD markers and 66.66% to 100% for SSR markers. These findings indicate that physical mutation induced by 10 Gy gamma irradiation can be clearly distinguished from chemical mutation induced by EMS at concentrations of 1% and 0.75% in chrysanthemum cv. "Candid.″ Overall, this study provides valuable insights into the genetic composition of the generated mutants and highlights their potential for enhancing chrysanthemum-breeding programs. The identified markers, particularly, OPA-07 and JH47, can serve as valuable tools for future studies aimed at exploring and exploiting the genetic diversity within the chrysanthemum population.
    Sprache Englisch
    Erscheinungsdatum 2023-11-10
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 2470-1343
    ISSN (online) 2470-1343
    DOI 10.1021/acsomega.3c05723
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  2. Artikel: Developing an efficient in vitro callusing and regeneration protocol in Dendranthema × grandiflorum Kitam

    Din, Ambreena / Qadri, Z. A. / Wani, Muneeb Ahmad / Iqbal, Shameen / Malik, Sajid Ali / Bhat, Z. A. / Banday, Neelofar

    Journal of crop science and biotechnology. 2022 Sept., v. 25, no. 4

    2022  

    Abstract: To standardize the methodology for in vitro callusing and regeneration of two chrysanthemum cultivars, ‘Candid’ and ‘Flirt,’ an experiment was carried out. The petals and leaf segments were used as explants. In both cultivars, petals were shown to be the ...

    Abstract To standardize the methodology for in vitro callusing and regeneration of two chrysanthemum cultivars, ‘Candid’ and ‘Flirt,’ an experiment was carried out. The petals and leaf segments were used as explants. In both cultivars, petals were shown to be the most effective for callusing and regeneration. In both cultivars, MS medium enriched with 1.00 mg L⁻¹ BAP + 1.50 mg L⁻¹ NAA resulted in the best callusing of petal explants in terms of minimal days to callus initiation, maximal callus induction, and callus weight per explant. When using petal explants from both cultivars, the shortest time (13.25 and 16.00 days) to callus initiation, the highest callus induction (91.66 and 83.33%) and the highest mean callus weight (2.43 and 2.31 g per explant) were all found in media augmented with 1.00 mg L⁻¹ BAP + 1.50 mg L⁻¹ NAA. In both cultivars, MS media supplied with 1.00 mg L⁻¹ Kinetin + 0.50 mg L⁻¹ IAA resulted in the best regeneration of petal explants in calli-producing shoots and shoot number per explant. Maximum calli-producing shoots 89.58 and 87.49% and significantly maximum shoot number 3.25 and 2.75 per explant were noticed in media fortified with Kinetin 1.00 mg L⁻¹ + IAA 0.50 mg L⁻¹ in case of petal explants of both the cultivars. Nevertheless, the finest callusing of leaf explants in terms of minimum days to callus initiation, highest callus induction in both cultivars was achieved using MS media supplied with 1.00 mg L⁻¹ BAP + 1.50 mg L⁻¹ NAA, whereas the highest callus weight per explant was acquired using MS media supplied with 0.50 mg L⁻¹ BAP and 2.50 mg L⁻¹ NAA. Explants from both cultivars showed a minimum callus initiation period of 15.25 and 16.75 days, callus induction rates of 89.57 and 81.24%, and maximum mean callus weights of 0.94 and 0.89 g, correspondingly.
    Schlagwörter Chrysanthemum ; biotechnology ; callus ; cultivars ; kinetin ; leaves
    Sprache Englisch
    Erscheinungsverlauf 2022-09
    Umfang p. 393-405.
    Erscheinungsort Springer Nature Singapore
    Dokumenttyp Artikel
    ZDB-ID 2300030-2
    ISSN 2005-8276 ; 1975-9479
    ISSN (online) 2005-8276
    ISSN 1975-9479
    DOI 10.1007/s12892-022-00140-w
    Datenquelle NAL Katalog (AGRICOLA)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Bonn

    Z 7743: Hefte anzeigen

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Zum Seitenanfang