LIVIVO - Search results -

Search results

Result 1 - 10 of total 27

Search options

Article ; Online: Sperm interaction with bacteria induces the spontaneous acrosome reaction

Azoulay, Yael / Malik, Zvi / Breitbart, Haim

Theriogenology. 2023 June, v. 203 p.82-88

2023

Abstract: Bacterial contamination in the semen deteriorates spermatozoa function. One mechanism through which this may occur is by inducing a premature form of the acrosome reaction (spontaneous acrosome reaction (sAR)) which has been shown to abrogate ... ...

Abstract	Bacterial contamination in the semen deteriorates spermatozoa function. One mechanism through which this may occur is by inducing a premature form of the acrosome reaction (spontaneous acrosome reaction (sAR)) which has been shown to abrogate fertilization. To understand the mechanism by which bacteria affect sperm functions, we determined the effects of bacteria on sperm sAR and on other parameters involved in sperm capacitation. Sperm cells undergo biochemical changes in the female reproductive tract collectively called capacitation. Only capacitated sperm can undergo the physiological acrosomal exocytosis process near or on the oocyte, which allows the spermatozoon to penetrate and fertilize the egg. Bovine sperm incubated with the bacteria Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) or Pseudomonas aeruginosa (P. aeruginosa), revealed a sperm-bacteria interaction, however only E. coli and P. aeruginosa caused an increase in sperm sAR. This effect was seen only when the bacteria were present with the sperm during the full incubation under capacitation conditions but not when the bacteria were added to capacitated sperm. These results indicate that bacteria affect sperm during capacitation and not at the AR step. In addition, Ca²⁺ influx, protein kinase A, and protein tyrosine phosphorylation activities, three essential processes that promote capacitation, were inhibited by the bacteria. Moreover, increasing intracellular cAMP, which also occur during sperm capacitation, caused significant reverse of sAR induced by the bacteria.
Keywords	Escherichia coli ; Pseudomonas aeruginosa ; Staphylococcus aureus ; acrosome reaction ; bacterial contamination ; cAMP-dependent protein kinase ; calcium ; cattle ; eggs ; exocytosis ; female reproductive system ; oocytes ; phosphorylation ; semen ; sperm capacitation ; spermatozoa ; tyrosine ; Bacteria ; Capacitation
Language	English
Dates of publication	2023-06
Size	p. 82-88.
Publishing place	Elsevier Inc.
Document type	Article ; Online
ZDB-ID	189232-0
ISSN	1879-3231 ; 0093-691X
ISSN (online)	1879-3231
ISSN	0093-691X
DOI	10.1016/j.theriogenology.2023.02.029
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 3929: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾

Article ; Online: Sperm interaction with bacteria induces the spontaneous acrosome reaction.

Azoulay, Yael / Malik, Zvi / Breitbart, Haim

Theriogenology

2023 Volume 203, Page(s) 82–88

Abstract	Bacterial contamination in the semen deteriorates spermatozoa function. One mechanism through which this may occur is by inducing a premature form of the acrosome reaction (spontaneous acrosome reaction (sAR)) which has been shown to abrogate fertilization. To understand the mechanism by which bacteria affect sperm functions, we determined the effects of bacteria on sperm sAR and on other parameters involved in sperm capacitation. Sperm cells undergo biochemical changes in the female reproductive tract collectively called capacitation. Only capacitated sperm can undergo the physiological acrosomal exocytosis process near or on the oocyte, which allows the spermatozoon to penetrate and fertilize the egg. Bovine sperm incubated with the bacteria Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) or Pseudomonas aeruginosa (P. aeruginosa), revealed a sperm-bacteria interaction, however only E. coli and P. aeruginosa caused an increase in sperm sAR. This effect was seen only when the bacteria were present with the sperm during the full incubation under capacitation conditions but not when the bacteria were added to capacitated sperm. These results indicate that bacteria affect sperm during capacitation and not at the AR step. In addition, Ca
MeSH term(s)	Male ; Cattle ; Animals ; Female ; Acrosome Reaction ; Semen ; Escherichia coli ; Staphylococcus aureus ; Spermatozoa ; Sperm Capacitation ; Acrosome/physiology
Language	English
Publishing date	2023-03-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	189232-0
ISSN	1879-3231 ; 0093-691X
ISSN (online)	1879-3231
ISSN	0093-691X
DOI	10.1016/j.theriogenology.2023.02.029
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 3929: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Pros, cons and future prospects of ALA-photodiagnosis, phototherapy and pharmacology in cancer therapy - A mini review

Malik, Zvi

Photonics & lasers in medicine

2015 Volume 4, Issue 1, Page(s) 19

Language	English
Document type	Article
ZDB-ID	2651139-3
ISSN	2193-0635
Database	Current Contents Medicine

In stock of ZB MED Cologne/Königswinter

Zs.A 6952: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: The synergistic effect of PDT and oxacillin on clinical isolates of Staphylococcus aureus.

Iluz, Natanel / Maor, Yasmin / Keller, Natan / Malik, Zvi

Lasers in surgery and medicine

2018 Volume 50, Issue 5, Page(s) 535–551

Abstract: Background: Staphylococcus aureus is a major pathogen in clinical microbiology. It is known to cause infections at various body sites and can be life-threatening. The development of resistance to many well-established antibiotic treatments and the ... ...

Abstract	Background: Staphylococcus aureus is a major pathogen in clinical microbiology. It is known to cause infections at various body sites and can be life-threatening. The development of resistance to many well-established antibiotic treatments and the prevalence of methicillin-resistant S. aureus (MRAS) among hospital patients and the general community pose challenges in treating the pathogen. The antimicrobial effect of photodynamic therapy (PDT) has been a subject of study for a long time and can offer new strategies for dealing with resistant strains. Objective: In our study, we searched for a positive synergistic relationship between PDT and the standard antibiotics used to treat S. aureus and MRSA infections. Materials and methods: The phototoxic profile of deuteroporphyrin (DP) in both resistant and susceptible clinical strains of S. aureus was determined by plating of treated and untreated broth cultures. Electron microscopy imaging was done to explore possible sites of damage and free-radical accumulation in the cells during DP-PDT. Minimal inhibitory concentration (MIC) of oxacillin, gentamicin, vancomycin, rifampin, and fusidic acid was determined using the broth dilution method, and the checkerboard method was used to detect and evaluate the synergistic potential of DP-PDT and antibiotic combinations. A synergistic combination was further characterized using broth cultures and plating. Results: DP-PDT using a light dose of 15 J/cm Conclusions: PDT with porphyrins shows possible new therapeutic options in treating drug-resistant S. aureus at body sites suitable for irradiation. The synergistic effect of DP-PDT with oxacillin on clinical strains illustrates the potential of PDT to augment traditional antibiotic treatment based on cell wall inhibitors. Lasers Surg. Med. 50:535-551, 2018. © 2018 Wiley Periodicals, Inc.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Deuteroporphyrins/pharmacology ; Drug Synergism ; Microbial Sensitivity Tests ; Oxacillin/pharmacology ; Photochemotherapy ; Photosensitizing Agents/pharmacology ; Staphylococcus aureus/drug effects
Chemical Substances	Anti-Bacterial Agents ; Deuteroporphyrins ; Photosensitizing Agents ; deuteroporphyrin-IX (448-65-7) ; Oxacillin (UH95VD7V76)
Language	English
Publishing date	2018-01-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	604493-1
ISSN	1096-9101 ; 0196-8092
ISSN (online)	1096-9101
ISSN	0196-8092
DOI	10.1002/lsm.22785
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1603: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Comparative kinetics of damage to the plasma and mitochondrial membranes by intra-cellularly synthesized and externally-provided photosensitizers using multi-color FACS.

Haupt, Sara / Malik, Zvi / Ehrenberg, Benjamin

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology

2014 Volume 13, Issue 1, Page(s) 38–47

Abstract: Photodynamic therapy (PDT) of cancer involves inflicting lethal damage to the cells of malignant tumors, primarily by singlet oxygen that is generated following light-absorption in a photosensitizer molecule. Dysfunction of cells is manifested in many ... ...

Abstract	Photodynamic therapy (PDT) of cancer involves inflicting lethal damage to the cells of malignant tumors, primarily by singlet oxygen that is generated following light-absorption in a photosensitizer molecule. Dysfunction of cells is manifested in many ways, including peroxidation of cellular components, membrane rupture, depolarization of electric potentials, termination of mitochondrial activity, onset of apoptosis and necrosis and eventually cell lysis. These events do not necessarily occur in linear fashion and different types of damage to cell components occur, most probably, in parallel. In this report we measured the relative rates of damage to two cellular membranes: the plasma membrane and the mitochondrial membrane. We employed photosensitizers of diverse hydrophobicities and used different incubation procedures, which lead to their different intra-cellular localizations. We monitored the damage that was inflicted on these membranes, by employing optical probes of membrane integrity, in a multi-color FACS experiment. The potentiometric indicator JC-1 monitored the electric cross-membrane potential of the mitochondria and the fluorometric indicator Draq7 monitored the rupture of the plasma membrane. We show that the electric depolarization of the mitochondrial membrane and the damage to the enveloping plasma membrane proceed with different kinetics that reflect the molecular character and intracellular location of the sensitizer: PpIX that is synthesized in the cells from ALA causes rapid mitochondrial damage and very slow damage to the plasma membrane, while externally added PpIX has an opposite effect. The hydrophilic sensitizer HypS4 can be taken up by the cells by different incubation conditions, and these affect its intracellular location, and as a consequence either the plasma membrane or the mitochondria is damaged first. A similar correlation was found for additional extracellularly-provided photosensitizers HP and PpIX.
MeSH term(s)	Aminolevulinic Acid/pharmacology ; Anthracyclines ; Benzimidazoles ; Carbocyanines ; Cell Line, Tumor ; Cell Membrane/drug effects ; Cell Survival/drug effects ; Flow Cytometry ; Fluorescent Dyes ; Humans ; Hydrophobic and Hydrophilic Interactions ; Kinetics ; Light ; Membrane Potential, Mitochondrial/drug effects ; Mitochondrial Membranes/drug effects ; Neurons/drug effects ; Neurons/pathology ; Photosensitizing Agents/pharmacology ; Protoporphyrins/pharmacology
Chemical Substances	Anthracyclines ; Benzimidazoles ; Carbocyanines ; DRAQ7 fluorescent probe ; Fluorescent Dyes ; Photosensitizing Agents ; Protoporphyrins ; 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine (21527-78-6) ; Aminolevulinic Acid (88755TAZ87) ; protoporphyrin IX (C2K325S808)
Language	English
Publishing date	2014-01
Publishing country	England
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2072584-X
ISSN	1474-9092 ; 1474-905X
ISSN (online)	1474-9092
ISSN	1474-905X
DOI	10.1039/c3pp50189b
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Pdots nanoparticles load photosensitizers and enhance efficiently their photodynamic effect by FRET

Haimov, Elina / Ehrenberg, Benjamin / Ickowicz, Debby / Malik, Zvi / Weitman, Hana

RSC advances. 2015 Feb. 12, v. 5, no. 24

2015

Abstract: A new type of nanoparticles, Pdots, and a new methodology of photosensitization are developed to achieve a more efficient photodynamic effect in aqueous solutions and in cells. Pdots are nano-sized particles, composed of conjugated chromophoric polymers ... ...

Abstract	A new type of nanoparticles, Pdots, and a new methodology of photosensitization are developed to achieve a more efficient photodynamic effect in aqueous solutions and in cells. Pdots are nano-sized particles, composed of conjugated chromophoric polymers coated with PEGylated phospholipids. They exhibit good aqueous colloidal properties, a broad absorption band and a strong and narrow emission band. We show that these characteristics improve biological photosensitization, which is employed in the photodynamic therapy of cancer. Amphiphilic photosensitizers such as Rose Bengal partition, non-covalently but with a high affinity, into the amphiphilic coating of the Pdots, without necessitating covalent attachment. At this close contact, very efficient fluorescence resonance energy transfer (FRET) occurs between the Pdot donor and the sensitizer acceptor. The Pdots serve as broad-band collectors of light, which is funneled, via energy transfer, to the photosensitizer. Therefore, energy transfer from them can additively assist in the activity of the acceptors of energy. The energy transfer mechanism, strong uptake of the Pdot-sensitizer dyads by MCF-7 adenocarcinoma cells and their enhanced photosensitized killing are demonstrated.
Keywords	absorption ; aqueous solutions ; coatings ; collectors ; colloidal properties ; energy transfer ; fluorescence ; nanoparticles ; neoplasm cells ; neoplasms ; phospholipids ; photochemotherapy ; photosensitivity ; photosensitizing agents ; polymers
Language	English
Dates of publication	2015-0212
Size	p. 18482-18491.
Publishing place	The Royal Society of Chemistry
Document type	Article
ISSN	2046-2069
DOI	10.1039/c4ra15291c
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: The centrality of PBGD expression levels on ALA-PDT efficacy.

Schauder, Avital / Feuerstein, Tamar / Malik, Zvi

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology

2011 Volume 10, Issue 8, Page(s) 1310–1317

Abstract: Successful 5-aminolevulinic acid-based photodynamic therapy (ALA-PDT) is dependent on efficient porphyrin synthesis in the inflicted cancer tissue, which is regulated by several enzymes. Irradiation of the tumor excites the light-sensitive porphyrins and ...

Abstract	Successful 5-aminolevulinic acid-based photodynamic therapy (ALA-PDT) is dependent on efficient porphyrin synthesis in the inflicted cancer tissue, which is regulated by several enzymes. Irradiation of the tumor excites the light-sensitive porphyrins and results in ROS production and cell death. In this study we investigated the effect of the expression levels of two main enzymes in heme biosynthesis, ALA dehydratase (ALAD) and porphobilinogen deaminase (PBGD), on the capacity of K562 cells to undergo cell death following ALA-PDT. We manipulated PBGD and ALAD expression levels by shRNAs and PBGD overexpressing plasmid. PBGD down-regulation induced an elevation in ALAD activity, while overexpression of PBGD reduced ALAD activity, indicating a novel regulation feedback of PBGD on ALAD activity. This feedback mechanism enabled partial PpIX synthesis under PBGD silencing, whereas ALAD silencing reduced PpIX production to a minimum. ALA-PDT efficacy was directly correlated to PpIX levels. Thus, only ALAD-silenced cells were not affected by ALA+ irradiation, while following PBGD silencing, the accumulated PpIX, though decreased, was sufficient for successful ALA-PDT. The alterations in ALAD activity level initiated by changes in PBGD expression indicates PBGD's central role in heme synthesis. This enables efficient ALA-PDT, even when PBGD is not fully active. Conversely, ALAD loss resulted in reduced PpIX synthesis and consequently failure in ALA-PDT, due to the absence of compensation mechanism for ALAD.
MeSH term(s)	Aminolevulinic Acid/chemistry ; Aminolevulinic Acid/pharmacology ; Aminolevulinic Acid/therapeutic use ; Apoptosis ; Gene Expression Regulation, Neoplastic ; Humans ; Hydroxymethylbilane Synthase/antagonists & inhibitors ; Hydroxymethylbilane Synthase/genetics ; Hydroxymethylbilane Synthase/metabolism ; K562 Cells ; Leukemia, Erythroblastic, Acute/drug therapy ; Leukemia, Erythroblastic, Acute/enzymology ; Light ; Photochemotherapy ; Porphobilinogen Synthase/antagonists & inhibitors ; Porphobilinogen Synthase/genetics ; Porphobilinogen Synthase/metabolism ; Protoporphyrins/metabolism ; RNA Interference ; RNA, Small Interfering/metabolism
Chemical Substances	Protoporphyrins ; RNA, Small Interfering ; Aminolevulinic Acid (88755TAZ87) ; protoporphyrin IX (C2K325S808) ; Hydroxymethylbilane Synthase (EC 2.5.1.61) ; Porphobilinogen Synthase (EC 4.2.1.24)
Language	English
Publishing date	2011-08
Publishing country	England
Document type	Journal Article
ZDB-ID	2072584-X
ISSN	1474-9092 ; 1474-905X
ISSN (online)	1474-9092
ISSN	1474-905X
DOI	10.1039/c1pp05085k
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Regulation and gene expression of heme synthesis under heavy metal exposure--review.

Schauder, Avital / Avital, Almog / Malik, Zvi

Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer

2010 Volume 29, Issue 2, Page(s) 137–158

Abstract: Environmental pollution of heavy metals is very abundant nowadays from industry, chemicals, old paints, and pipes or resulting from previous contaminants accumulating in the food chain. Most of the iron demands of the body are needed for heme synthesis ... ...

Abstract	Environmental pollution of heavy metals is very abundant nowadays from industry, chemicals, old paints, and pipes or resulting from previous contaminants accumulating in the food chain. Most of the iron demands of the body are needed for heme synthesis and assembly, but iron is also required for Fe-S cluster proteins and other redox enzymes. Heme is an essential, iron-binding molecule used as a prosthetic group of hemoproteins or as a regulator in multiple cellular pathways. In this review, we focused on the effect of exposure to heavy metals, such as Pb, Ga, Cu, Kd, Hg and Al, on heme synthesis as the main iron-sequestering process of the human body. These metals compete with iron on transporters, reduce the cellular iron pool and moreover, bind to proteins, and cause physical and mental disturbances. Heavy metals mainly impair various aspects of the heme synthesis pathway: gene expression, enzyme activity, and iron integration into protoporphyrin IX. Main risk factors are described as well as effects on iron dependent processes in order to increase public awareness to the distribution of heavy metals in our close environment and the harsh consequences of exposure, even in low doses.
MeSH term(s)	5-Aminolevulinate Synthetase/genetics ; Animals ; Cadmium/toxicity ; Copper/toxicity ; Ferrochelatase/genetics ; Gene Expression Regulation/drug effects ; Heme/biosynthesis ; Heme Oxygenase (Decyclizing)/genetics ; Homeostasis ; Humans ; Hydroxymethylbilane Synthase/genetics ; Iron/metabolism ; Lead Poisoning/metabolism ; Metals, Heavy/toxicity ; Photochemotherapy ; Porphobilinogen Synthase/genetics
Chemical Substances	Metals, Heavy ; Cadmium (00BH33GNGH) ; Heme (42VZT0U6YR) ; Copper (789U1901C5) ; Iron (E1UOL152H7) ; Heme Oxygenase (Decyclizing) (EC 1.14.14.18) ; 5-Aminolevulinate Synthetase (EC 2.3.1.37) ; Hydroxymethylbilane Synthase (EC 2.5.1.61) ; Porphobilinogen Synthase (EC 4.2.1.24) ; Ferrochelatase (EC 4.99.1.1)
Language	English
Publishing date	2010-10-07
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	441790-2
ISSN	2162-6537 ; 0731-8898 ; 0146-4779
ISSN (online)	2162-6537
ISSN	0731-8898 ; 0146-4779
DOI	10.1615/jenvironpatholtoxicoloncol.v29.i2.70
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1472: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Regulation of heme synthesis and proteasomal activity by copper: possible implications for Wilson's disease.

Hait-Darshan, Ravit / Babushkin, Tania / Malik, Zvi

Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer

2009 Volume 28, Issue 3, Page(s) 209–221

Abstract: Wilson's disease (Wd) is a genetic disorder resulting in Cu2+ accumulation, and is caused by mutations in the ATP7B gene, the copper transporter. In vivo studies show a correlation between Cu2+ accumulation and malfunction of the heme biosynthesis ... ...

Abstract	Wilson's disease (Wd) is a genetic disorder resulting in Cu2+ accumulation, and is caused by mutations in the ATP7B gene, the copper transporter. In vivo studies show a correlation between Cu2+ accumulation and malfunction of the heme biosynthesis pathway. In this study, we describe multiple effects of Cu2+ accumulation on heme synthesis, which, in turn, affect proteasomal activity. Cu2+ toxicity was examined in two hepatocellular carcinoma cell lines, HepG2 and Hep3B, with Hep3B cells containing an integrated hepatitis B virus genome. Exposure of HepG2 and Hep3B cells to Cu2+ inhibited the enzymes PBGD and ALAD of the heme synthesis pathway and, in parallel, upregulated heme oxygenase-1 (HO-1). Proto-porphyrin IX (PpIX) and the heme pool were reduced as a result of these processes. PpIX synthesis was found to be lower in cells expressing the mutant ATP7B (P1134P), compared to those expressing the WT enzyme. Proteasomal activity was inhibited under Cu2+ treatment in HepG2 cells; however, Cu2+ induced marked proteosomal acceleration in Hep3B cells. Under these conditions, Ub-conjugated proteins were gradually accumulated, whereas treatment with bathocuproine disulfonic acid (BCS), a Cu2+ chelator, reversed this effect. In conclusion, our data suggest that copper downregulates the heme synthesis pathway in hepatocellular cells and further reduces it in the presence of mutated ATP7B.
MeSH term(s)	Adenosine Triphosphatases/biosynthesis ; Carcinoma, Hepatocellular/drug therapy ; Carcinoma, Hepatocellular/metabolism ; Cation Transport Proteins/biosynthesis ; Cell Line, Tumor ; Cell Survival/drug effects ; Copper/toxicity ; Copper-transporting ATPases ; Disulfiram/pharmacology ; Down-Regulation/drug effects ; Enzyme Inhibitors/toxicity ; Heme/biosynthesis ; Hepatocytes/drug effects ; Hepatocytes/metabolism ; Hepatolenticular Degeneration ; Humans ; Hydroxymethylbilane Synthase/antagonists & inhibitors ; Liver Neoplasms/drug therapy ; Liver Neoplasms/metabolism ; Porphobilinogen Synthase/antagonists & inhibitors ; Proteasome Endopeptidase Complex/drug effects ; Proteasome Endopeptidase Complex/metabolism ; Protoporphyrins/metabolism ; Reactive Oxygen Species/metabolism ; Ubiquitin/metabolism
Chemical Substances	Cation Transport Proteins ; Enzyme Inhibitors ; Protoporphyrins ; Reactive Oxygen Species ; Ubiquitin ; Heme (42VZT0U6YR) ; Copper (789U1901C5) ; protoporphyrin IX (C2K325S808) ; Hydroxymethylbilane Synthase (EC 2.5.1.61) ; Proteasome Endopeptidase Complex (EC 3.4.25.1) ; Adenosine Triphosphatases (EC 3.6.1.-) ; Copper-transporting ATPases (EC 3.6.3.54) ; Porphobilinogen Synthase (EC 4.2.1.24) ; ATP7B protein, human (EC 7.2.2.8) ; cupric chloride (S2QG84156O) ; Disulfiram (TR3MLJ1UAI)
Language	English
Publishing date	2009-10-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	441790-2
ISSN	2162-6537 ; 0731-8898 ; 0146-4779
ISSN (online)	2162-6537
ISSN	0731-8898 ; 0146-4779
DOI	10.1615/jenvironpatholtoxicoloncol.v28.i3.20
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1472: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Silencing of ALA dehydratase affects ALA-photodynamic therapy efficacy in K562 erythroleukemic cells.

Feuerstein, Tamar / Schauder, Avital / Malik, Zvi

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology

2009 Volume 8, Issue 10, Page(s) 1461–1466

Abstract: Synthesis of protoporphyrin IX (PpIX) by malignant cells is essential for the success of ALA-based photodynamic therapy (PDT). Two key enzymes that were described as affecting PpIX accumulation during ALA treatment are porphobilinogen deaminase (PBGD) ... ...

Abstract	Synthesis of protoporphyrin IX (PpIX) by malignant cells is essential for the success of ALA-based photodynamic therapy (PDT). Two key enzymes that were described as affecting PpIX accumulation during ALA treatment are porphobilinogen deaminase (PBGD) and ferrochelatase. Here, we show that down regulation of ALA dehydratase (ALAD) expression and activity by specific shRNA induced a marked decrease in PpIX synthesis in K562 erythroleukemic cells. Photo-inactivation efficacy following ALA-PDT was directly correlated with ALAD-silencing and cellular levels of PpIX. MTT metabolism following ALA-PDT was shown to be 60% higher in ALAD-silenced cells in comparison to control cells, indicating that mitochondria were protected in the silenced cells. Morphological analysis by scanning electron microscopy (SEM) of cells treated by ALA-PDT showed no morphological changes in ALAD-silenced cells, in contrast to controls exhibiting cell deformations and lysis. Membrane integrity following ALA-PDT was kept intact and undamaged in ALAD-silenced cells as examined by Annexin V-FITC/PI staining and LDH-L leakage. We conclude that ALAD, although it is present in the cell at abundant levels, has a major and limiting role in regulating PpIX synthesis and ALA-PDT outcome.
MeSH term(s)	Aminolevulinic Acid/pharmacology ; Aminolevulinic Acid/therapeutic use ; Cell Death/drug effects ; Cell Death/radiation effects ; Gene Silencing ; Humans ; K562 Cells ; Leukemia, Erythroblastic, Acute/pathology ; Mitochondria/drug effects ; Mitochondria/metabolism ; Mitochondria/radiation effects ; Photochemotherapy ; Porphobilinogen Synthase/deficiency ; Porphobilinogen Synthase/genetics ; Protoporphyrins/biosynthesis
Chemical Substances	Protoporphyrins ; Aminolevulinic Acid (88755TAZ87) ; protoporphyrin IX (C2K325S808) ; Porphobilinogen Synthase (EC 4.2.1.24)
Language	English
Publishing date	2009-10
Publishing country	England
Document type	Journal Article
ZDB-ID	2072584-X
ISSN	1474-9092 ; 1474-905X
ISSN (online)	1474-9092
ISSN	1474-905X
DOI	10.1039/b9pp00007k
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito