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  1. Article: In vivo matrigel migration and angiogenesis assay.

    Malinda, Katherine M

    Methods in molecular biology (Clifton, N.J.)

    2009  Volume 467, Page(s) 287–294

    Abstract: The search for rapid and reproducible in vivo angiogenesis and antiangiogenesis assays is an area of intense interest. These types of assays are extremely useful in testing putative drugs and biological agents and for the comparison and enhancement of in ...

    Abstract The search for rapid and reproducible in vivo angiogenesis and antiangiogenesis assays is an area of intense interest. These types of assays are extremely useful in testing putative drugs and biological agents and for the comparison and enhancement of in vitro tests. The Matrigel plug assay is one such assay and has proved to be a relatively quick and easy method to evaluate both angiogenic and antiangiogenic compounds in vivo. Initial indications of the levels of activity of strong angiogenic or antiangiogenic compounds can be visually assessed even as the plugs come out of the mouse because there are color differences in the plugs compared to the controls. Further quantitation is then needed to determine levels of angiogenic/antiangiogenic activity, and this can be performed using a variety of methods. This chapter presents an overview of the basic methods used to set up both angiogenic and antiangiogenic assays, discusses factors influencing variability, and discusses the methods for quantitating the plugs obtained. The Matrigel plug assay provides another useful tool in angiogenesis research.
    MeSH term(s) Animals ; Biological Assay/methods ; Collagen/metabolism ; Drug Combinations ; Female ; Laminin/metabolism ; Mice ; Mice, Inbred C57BL ; Neovascularization, Physiologic ; Proteoglycans/metabolism
    Chemical Substances Drug Combinations ; Laminin ; Proteoglycans ; matrigel (119978-18-6) ; Collagen (9007-34-5)
    Language English
    Publishing date 2009
    Publishing country United States
    Document type Journal Article
    ISSN 1064-3745
    ISSN 1064-3745
    DOI 10.1007/978-1-59745-241-0_17
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: In vivo matrigel migration and angiogenesis assays.

    Malinda, Katherine M

    Methods in molecular medicine

    2003  Volume 78, Page(s) 329–335

    MeSH term(s) Animals ; Cell Movement ; Collagen/pharmacology ; Drug Combinations ; Laminin/pharmacology ; Mice ; Models, Animal ; Neovascularization, Physiologic ; Proteoglycans/pharmacology
    Chemical Substances Drug Combinations ; Laminin ; Proteoglycans ; matrigel (119978-18-6) ; Collagen (9007-34-5)
    Language English
    Publishing date 2003
    Publishing country United States
    Document type Journal Article
    ISSN 1543-1894
    ISSN 1543-1894
    DOI 10.1385/1-59259-332-1:329
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Angiogenic laminin-derived peptides stimulate wound healing.

    Malinda, Katherine M / Wysocki, Annette B / Koblinski, Jennifer E / Kleinman, Hynda K / Ponce, M Lourdes

    The international journal of biochemistry & cell biology

    2008  Volume 40, Issue 12, Page(s) 2771–2780

    Abstract: Acceleration of the wound healing process by using angiogenic peptides has been demonstrated previously. Here we used select laminin-111 peptides, A13 and C16, from the laminin alpha1 and gamma1 chain, respectively, to test whether they are able to ... ...

    Abstract Acceleration of the wound healing process by using angiogenic peptides has been demonstrated previously. Here we used select laminin-111 peptides, A13 and C16, from the laminin alpha1 and gamma1 chain, respectively, to test whether they are able to stimulate wound healing in a rat full thickness wound model. The 12-mer peptides C16 and A13 are highly angiogenic and bind to integrins alphavbeta3 and alpha5beta1. We show that A13 increases wound re-epithelialization as much as 17% over controls by day 4 and C16 increases coverage by 11%. Contraction of the treated wounds was increased as much as 11% for A13 and 8% for C16 at day 4. No differences were observed at day 7 with either peptide. The peptides also stimulated fibroblast migration in Boyden chamber assays. A13 increased cell migration as much as 2.4-fold on uncoated filters and as much as 16-fold on collagen type IV-coated filters over negative controls. Similarly, C16 also stimulated migration 1.8-fold on uncoated filters and as much as 12-fold on collagen-coated filters. A13 and C16 significantly decreased expression of the pro and active forms of matrix metalloproteinase 2 in foreskin fibroblasts indicating their role in collagen accumulation. We conclude that small bioactive angiogenic peptides can promote dermal wound healing and may offer a new class of stable and chemically manipulable therapeutics for wound healing.
    MeSH term(s) Angiogenic Proteins/chemistry ; Angiogenic Proteins/metabolism ; Angiogenic Proteins/pharmacology ; Animals ; Cell Movement/drug effects ; Cell Movement/physiology ; Cells, Cultured ; Collagen Type IV/metabolism ; Dose-Response Relationship, Drug ; Endothelium/drug effects ; Endothelium/metabolism ; Endothelium/physiology ; Endothelium, Vascular/cytology ; Fibroblasts/drug effects ; Fibroblasts/metabolism ; Fibroblasts/physiology ; Humans ; Integrin alpha5beta1/metabolism ; Integrin alphaVbeta3/metabolism ; Laminin/chemistry ; Laminin/physiology ; Matrix Metalloproteinase 2/metabolism ; Neovascularization, Physiologic/drug effects ; Neovascularization, Physiologic/physiology ; Rats ; Skin/cytology ; Time Factors ; Umbilical Veins/cytology ; Wound Healing/drug effects ; Wound Healing/physiology
    Chemical Substances Angiogenic Proteins ; Collagen Type IV ; Integrin alpha5beta1 ; Integrin alphaVbeta3 ; Laminin ; laminin A (151186-83-3) ; Matrix Metalloproteinase 2 (EC 3.4.24.24)
    Language English
    Publishing date 2008-06-20
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1228429-4
    ISSN 1878-5875 ; 1357-2725
    ISSN (online) 1878-5875
    ISSN 1357-2725
    DOI 10.1016/j.biocel.2008.05.025
    Database MEDical Literature Analysis and Retrieval System OnLINE

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