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  1. Article ; Online: Use of PCR-DGGE-Based Molecular Methods to Analyze Nematode Community Diversity.

    Festa, Margherita / Abbruscato, Pamela / Manachini, Barbara

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2756, Page(s) 247–255

    Abstract: DGGE (denaturing gradient gel electrophoresis) is a nucleic acid separation technique applied to the evaluation of microbial biodiversity. This technique is quite rapid and cheap compared to other types of analysis. Here we describe the comparison of ... ...

    Abstract DGGE (denaturing gradient gel electrophoresis) is a nucleic acid separation technique applied to the evaluation of microbial biodiversity. This technique is quite rapid and cheap compared to other types of analysis. Here we describe the comparison of nematode communities inhabiting different ecosystems. After an ecologically representative sampling collection and the nematode extraction from soil, nematodes are centrifuged in Eppendorf tubes to facilitate DNA extraction. DNA from the whole community of each type of soil is extracted, amplified with primers for 18 S rDNA and used in DGGE analysis. The profiles of DGGE can be analyzed with appropriate software, and biodiversity indices can be estimated.
    MeSH term(s) Animals ; Ecosystem ; Biodiversity ; DNA, Ribosomal/genetics ; Polymerase Chain Reaction/methods ; Nematoda/genetics ; Soil ; Electrophoresis, Polyacrylamide Gel ; DNA, Bacterial/genetics ; RNA, Ribosomal, 16S/genetics ; Soil Microbiology
    Chemical Substances DNA, Ribosomal ; Soil ; DNA, Bacterial ; RNA, Ribosomal, 16S
    Language English
    Publishing date 2024-03-01
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3638-1_6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Heat Shock Proteins as Potential Indicators of Induced Stress in Nematodes.

    Celi, Monica / Vazzana, Mirella / Manachini, Barbara

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2756, Page(s) 343–350

    Abstract: Heat shock proteins (HSPs) in all animals studied to date constitute potential indicators of stress, under various environmental conditions. The goal of this chapter is to show, for the first time, the suitability of the approach based on evaluation of ... ...

    Abstract Heat shock proteins (HSPs) in all animals studied to date constitute potential indicators of stress, under various environmental conditions. The goal of this chapter is to show, for the first time, the suitability of the approach based on evaluation of the expression levels of heat shock proteins, as good indicators of stress induced in nematodes by the cultivation of resistant plant varieties or by other potential stressors.
    MeSH term(s) Animals ; Heat-Shock Proteins/metabolism ; Nematoda/metabolism ; Plant Proteins/metabolism ; Heat-Shock Response ; HSP70 Heat-Shock Proteins/metabolism
    Chemical Substances Heat-Shock Proteins ; Plant Proteins ; HSP70 Heat-Shock Proteins
    Language English
    Publishing date 2024-03-01
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3638-1_13
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  3. Article: Essential Oils as Nematicides in Plant Protection-A Review.

    Catani, Linda / Manachini, Barbara / Grassi, Eleonora / Guidi, Loretta / Semprucci, Federica

    Plants (Basel, Switzerland)

    2023  Volume 12, Issue 6

    Abstract: By 2030, the European Commission intends to halve chemical pesticide use and its consequent risks. Among pesticides, nematicides are chemical agents used to control parasitic roundworms in agriculture. In recent decades, researchers have been looking for ...

    Abstract By 2030, the European Commission intends to halve chemical pesticide use and its consequent risks. Among pesticides, nematicides are chemical agents used to control parasitic roundworms in agriculture. In recent decades, researchers have been looking for more sustainable alternatives with the same effectiveness but a limited impact on the environment and ecosystems. Essential oils (EOs) are similar bioactive compounds and potential substitutes. Different studies on the use of EOs as nematicides are available in the Scopus database in the scientific literature. These works show a wider exploration of EO effects in vitro than in vivo on different nematode populations. Nevertheless, a review of which EOs have been used on different target nematodes, and how, is still not available. The aim of this paper is to explore the extent of EO testing on nematodes and which of them have nematicidal effects (e.g., mortality, effects on motility, inhibition of egg production). Particularly, the review aims to identify which EOs have been used the most, on which nematodes, and which formulations have been applied. This study provides an overview of the available reports and data to date, downloaded from Scopus, through (a) network maps created by VOSviewer software (version 1.6.8, Nees Jan van Eck and Ludo Waltman, Leiden, The Netherlands) and (b) a systematic analysis of all scientific papers. VOSviewer created maps with keywords derived from co-occurrence analysis to understand the main keywords used and the countries and journals which have published most on the topic, while the systematic analysis investigated all the documents downloaded. The main goal is to offer a comprehensive understanding of the potential use of EOs in agriculture as well as which directions future research should move toward.
    Language English
    Publishing date 2023-03-22
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants12061418
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Does

    Celi, Monica / Russo, Debora / Vazzana, Mirella / Arizza, Vincenzo / Manachini, Barbara

    Insects

    2022  Volume 13, Issue 5

    Abstract: ... Bacillus ... ...

    Abstract Bacillus thuringiensis
    Language English
    Publishing date 2022-05-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662247-6
    ISSN 2075-4450
    ISSN 2075-4450
    DOI 10.3390/insects13050437
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  5. Article ; Online: Could Europe Apply a Suitable Control Method for the Small Hive Beetle (Coleoptera: Nitidulidae)?

    Sabella, Giorgio / Mulè, Rosaria / Robba, Lavinia / Agrò, Alfonso / Manachini, Barbara

    Journal of economic entomology

    2022  Volume 115, Issue 2, Page(s) 401–411

    Abstract: The European bee, Apis mellifera L. (Hymenoptera: Apidae), is a fundamental resource for the pollination of a great variety of botanical species used by humans for sustenance. Over the last few decades, bee colonies have become vulnerable to a new pest ... ...

    Abstract The European bee, Apis mellifera L. (Hymenoptera: Apidae), is a fundamental resource for the pollination of a great variety of botanical species used by humans for sustenance. Over the last few decades, bee colonies have become vulnerable to a new pest that has advanced beyond its native sub-Saharan territory: the small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae). This currently presents a pressing problem in the United States and Australia, but it has also been recorded in Portugal and Italy and it is likely to spread in the rest of Europe too. This study represents a systematic review, based on EFSA guidelines, of the various control treatments for small hive beetles in order to identify the most effective methods as well as, those with no effects on bee colonies. The results show that the bulk of these studies were performed in the United States and that a number of treatments are suitable for the control of A. tumida, though some have negative effects on bees while others have low effectiveness or are ineffective. The best results are those with the entomopathogenic nematodes of the genus Steinernema and Heterorhabditis, but also with formic acid or diatomaceous earth. Various products containing insecticides have been effective, for example, Perizin (Bayer), GardStar (Y-Tex), CheckMite+ strips (Bayer), but Apithor (Apithor ) cannot be used in Europe because it contains Fipronil, which has been banned since 2013. Some common products like bleach and detergent have also been effective.
    MeSH term(s) Animals ; Australia ; Bees ; Coleoptera ; Europe ; Insecticides ; Italy
    Chemical Substances Insecticides
    Language English
    Publishing date 2022-02-25
    Publishing country England
    Document type Journal Article ; Systematic Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 3031-4
    ISSN 1938-291X ; 0022-0493
    ISSN (online) 1938-291X
    ISSN 0022-0493
    DOI 10.1093/jee/toac001
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    In stock of ZB MED Cologne/Königswinter

    Zs.B 1118: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Novel [1,3,4]Thiadiazole[3,2-

    Carbone, Daniela / Pecoraro, Camilla / Scianò, Fabio / Catania, Valentina / Schillaci, Domenico / Manachini, Barbara / Cascioferro, Stella / Diana, Patrizia / Parrino, Barbara

    Marine drugs

    2024  Volume 22, Issue 3

    Abstract: Biofilm-associated infections pose significant challenges in healthcare settings due to their resistance to conventional antimicrobial therapies. In the last decade, the marine environment has been a precious source of bioactive molecules, including ... ...

    Abstract Biofilm-associated infections pose significant challenges in healthcare settings due to their resistance to conventional antimicrobial therapies. In the last decade, the marine environment has been a precious source of bioactive molecules, including numerous derivatives with antibiofilm activity. In this study, we reported the synthesis and the biological evaluation of a new series of twenty-two thiadiazopyrimidinone derivatives obtained by using a hybridization approach combining relevant chemical features of two important classes of marine compounds: nortopsentin analogues and Essramycin derivatives. The synthesized compounds were in vitro tested for their ability to inhibit biofilm formation and to disrupt mature biofilm in various bacterial strains. Among the tested compounds, derivative
    MeSH term(s) Thiadiazoles ; Biofilms ; Biological Assay ; Candida albicans ; Hybridization, Genetic
    Chemical Substances Thiadiazoles
    Language English
    Publishing date 2024-03-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2175190-0
    ISSN 1660-3397 ; 1660-3397
    ISSN (online) 1660-3397
    ISSN 1660-3397
    DOI 10.3390/md22030133
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Could Europe Apply a Suitable Control Method for the Small Hive Beetle (Coleoptera: Nitidulidae)?

    Sabella, Giorgio / Mulè, Rosaria / Robba, Lavinia / Agrò, Alfonso / Manachini, Barbara

    Journal of economic entomology. 2022 Feb. 26, v. 115, no. 2

    2022  

    Abstract: The European bee, Apis mellifera L. (Hymenoptera: Apidae), is a fundamental resource for the pollination of a great variety of botanical species used by humans for sustenance. Over the last few decades, bee colonies have become vulnerable to a new pest ... ...

    Abstract The European bee, Apis mellifera L. (Hymenoptera: Apidae), is a fundamental resource for the pollination of a great variety of botanical species used by humans for sustenance. Over the last few decades, bee colonies have become vulnerable to a new pest that has advanced beyond its native sub-Saharan territory: the small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae). This currently presents a pressing problem in the United States and Australia, but it has also been recorded in Portugal and Italy and it is likely to spread in the rest of Europe too. This study represents a systematic review, based on EFSA guidelines, of the various control treatments for small hive beetles in order to identify the most effective methods as well as, those with no effects on bee colonies. The results show that the bulk of these studies were performed in the United States and that a number of treatments are suitable for the control of A. tumida, though some have negative effects on bees while others have low effectiveness or are ineffective. The best results are those with the entomopathogenic nematodes of the genus Steinernema and Heterorhabditis, but also with formic acid or diatomaceous earth. Various products containing insecticides have been effective, for example, Perizin (Bayer), GardStar (Y-Tex), CheckMite+ strips (Bayer), but Apithor (Apithor) cannot be used in Europe because it contains Fipronil, which has been banned since 2013. Some common products like bleach and detergent have also been effective.
    Keywords Aethina tumida ; Apis mellifera ; Heterorhabditis ; Steinernema ; bees ; control methods ; detergents ; diatomaceous earth ; entomology ; entomopathogenic nematodes ; fipronil ; formic acid ; pests ; pollination ; systematic review ; Australia ; Italy ; Portugal
    Language English
    Dates of publication 2022-0226
    Size p. 401-411.
    Publishing place Entomological Society of America
    Document type Article
    ZDB-ID 3031-4
    ISSN 0022-0493
    ISSN 0022-0493
    DOI 10.1093/jee/toac001
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 1776: Show issues

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  8. Article: Odorants of

    Guarino, Salvatore / Basile, Sara / Arif, Mokhtar Abdulsattar / Manachini, Barbara / Peri, Ezio

    Insects

    2021  Volume 12, Issue 1

    Abstract: The cigarette beetle, ...

    Abstract The cigarette beetle,
    Language English
    Publishing date 2021-01-12
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2075-4450
    ISSN 2075-4450
    DOI 10.3390/insects12010061
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Potential impact of genetically modified Lepidoptera‐resistant Brassica napus in biodiversity hotspots: Sicily as a theoretical model

    Manachini, Barbara / Giuseppe Bazan / Rosario Schicchi

    Insect science. 2018 Aug., v. 25, no. 4

    2018  

    Abstract: The general increase of the cultivation and trade of Bt transgenic plants resistant to Lepidoptera pests raises concerns regarding the conservation of animal and plant biodiversity. Demand for biofuels has increased the cultivation and importation of ... ...

    Abstract The general increase of the cultivation and trade of Bt transgenic plants resistant to Lepidoptera pests raises concerns regarding the conservation of animal and plant biodiversity. Demand for biofuels has increased the cultivation and importation of oilseed rape (Brassica napus L.), including transgenic lines. In environmental risk assessments (ERAs) for its potential future cultivation as well as for food and feed uses, the impact on wild Brassicaeae relatives and on non‐target Lepidoptera should be assessed. Here we consider the potential exposure of butterflies as results of possible cultivation or naturalization of spilled seed in Sicily (Italy). Diurnal Lepidoptera, which are pollinators, can be exposed directly to the insecticidal proteins as larvae (mainly of Pieridae) through the host and through the pollen that can deposit on other host plants. Adults can be exposed via pollen and nectar. The flight periods of butterflies were recorded, and they were found to overlap for about 90% of the flowering period of B. napus for the majority of the species. In addition, B. napus has a high potential to hybridise with endemic taxa belonging to the B. oleracea group. This could lead to an exposure of non‐target Lepidoptera if introgression of the Bt gene into a wild population happens. A rank of the risk for butterflies and wild relatives of oilseed rape is given. We conclude that, in environmental risk assessments, attention should be paid to plant–insect interaction especially in a biodiversity hotspot such as Sicily.
    Keywords Bacillus thuringiensis ; Brassica napus ; Brassica oleracea ; Pieridae ; adults ; biodiversity ; biofuels ; butterflies ; environmental assessment ; flight ; flowering ; genes ; host plants ; insecticidal proteins ; introgression ; larvae ; nectar ; pests ; plant-insect relations ; pollen ; pollinators ; risk assessment ; theoretical models ; trade ; transgenic plants ; wild relatives ; Italy ; Sicily
    Language English
    Dates of publication 2018-08
    Size p. 562-580.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 2179775-4
    ISSN 1744-7917 ; 1672-9609
    ISSN (online) 1744-7917
    ISSN 1672-9609
    DOI 10.1111/1744-7917.12588
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  10. Article ; Online: Potential impact of genetically modified Lepidoptera-resistant Brassica napus in biodiversity hotspots: Sicily as a theoretical model.

    Manachini, Barbara / Bazan, Giuseppe / Schicchi, Rosario

    Insect science

    2018  Volume 25, Issue 4, Page(s) 562–580

    Abstract: The general increase of the cultivation and trade of Bt transgenic plants resistant to Lepidoptera pests raises concerns regarding the conservation of animal and plant biodiversity. Demand for biofuels has increased the cultivation and importation of ... ...

    Abstract The general increase of the cultivation and trade of Bt transgenic plants resistant to Lepidoptera pests raises concerns regarding the conservation of animal and plant biodiversity. Demand for biofuels has increased the cultivation and importation of oilseed rape (Brassica napus L.), including transgenic lines. In environmental risk assessments (ERAs) for its potential future cultivation as well as for food and feed uses, the impact on wild Brassicaeae relatives and on non-target Lepidoptera should be assessed. Here we consider the potential exposure of butterflies as results of possible cultivation or naturalization of spilled seed in Sicily (Italy). Diurnal Lepidoptera, which are pollinators, can be exposed directly to the insecticidal proteins as larvae (mainly of Pieridae) through the host and through the pollen that can deposit on other host plants. Adults can be exposed via pollen and nectar. The flight periods of butterflies were recorded, and they were found to overlap for about 90% of the flowering period of B. napus for the majority of the species. In addition, B. napus has a high potential to hybridise with endemic taxa belonging to the B. oleracea group. This could lead to an exposure of non-target Lepidoptera if introgression of the Bt gene into a wild population happens. A rank of the risk for butterflies and wild relatives of oilseed rape is given. We conclude that, in environmental risk assessments, attention should be paid to plant-insect interaction especially in a biodiversity hotspot such as Sicily.
    MeSH term(s) Animals ; Biodiversity ; Brassica napus/chemistry ; Brassica napus/genetics ; Brassica napus/physiology ; Butterflies/growth & development ; Models, Theoretical ; Plant Nectar/chemistry ; Plants, Genetically Modified/adverse effects ; Pollen/chemistry ; Risk Assessment ; Sicily
    Chemical Substances Plant Nectar
    Language English
    Publishing date 2018-04-26
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 2179775-4
    ISSN 1744-7917 ; 1672-9609
    ISSN (online) 1744-7917
    ISSN 1672-9609
    DOI 10.1111/1744-7917.12588
    Database MEDical Literature Analysis and Retrieval System OnLINE

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