LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 7 of total 7

Search options

  1. Article ; Online: Optimized synthesis of polyacrylic acid-coated magnetic nanoparticles for high-efficiency DNA isolation and size selection.

    Bali, Nesrine / Brennhaug, Svein J / Bjørås, Magnar / Bandyopadhyay, Sulalit / Manaf, Adeel

    RSC advances

    2023  Volume 13, Issue 42, Page(s) 29109–29120

    Abstract: Solid-phase reversible immobilization (SPRI) bead technology is widely used in molecular biology for convenient DNA manipulation. However, commercial SPRI bead kits lack cost advantages and flexibility. It is, therefore, necessary to develop new and ... ...

    Abstract Solid-phase reversible immobilization (SPRI) bead technology is widely used in molecular biology for convenient DNA manipulation. However, commercial SPRI bead kits lack cost advantages and flexibility. It is, therefore, necessary to develop new and alternative cost-effective methods of on-par or better quality. Herein, an easy and cost-effective method is proposed for synthesizing polyacrylic acid-coated iron oxide nanoparticles (PAA-IONPs) through
    Language English
    Publishing date 2023-10-04
    Publishing country England
    Document type Journal Article
    ISSN 2046-2069
    ISSN (online) 2046-2069
    DOI 10.1039/d3ra04687g
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article ; Online: NAxtra magnetic nanoparticles for low-cost, efficient isolation of mammalian DNA and RNA.

    Starheim, Eirin Johannessen / Ravlo, Erlend / Schjølberg, Jørn-Ove / Solvang, Vanessa / Wang, Wei / Scrimgeour, Nathan Robert / Manaf, Adeel / Erlandsen, Sten Even / Aas, Per Arne / Hagen, Lars / de Sousa, Mirta Mittelstedt Leal / Bjørås, Magnar

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 20836

    Abstract: A cost-effective, viral nucleic acid (NA) isolation kit based on NAxtra magnetic nanoparticles was developed at the Norwegian University of Science and Technology in response to the shortage of commercial kits for isolation of severe acute respiratory ... ...

    Abstract A cost-effective, viral nucleic acid (NA) isolation kit based on NAxtra magnetic nanoparticles was developed at the Norwegian University of Science and Technology in response to the shortage of commercial kits for isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA during the coronavirus disease 2019 (COVID-19) pandemic. This method showed comparable sensitivity to available kits at significantly reduced cost, making its application for other biological sources an intriguing prospect. Thus, based on this low-cost nucleic acid extraction technology, we developed a simple, low- and high-throughput, efficient method for isolation of high-integrity total NA, DNA and RNA from mammalian cell lines (monolayer) and organoids (3D-cultures). The extracted NA are compatible with downstream applications including (RT-)qPCR and next-generation sequencing. When automated, NA isolation can be performed in 14 min for up to 96 samples, yielding similar quantities to available kits.
    MeSH term(s) Animals ; Humans ; Magnetite Nanoparticles ; RNA, Viral/analysis ; COVID-19 ; SARS-CoV-2/genetics ; DNA ; Sensitivity and Specificity ; Mammals/genetics
    Chemical Substances Magnetite Nanoparticles ; RNA, Viral ; DNA (9007-49-2)
    Language English
    Publishing date 2023-11-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-46868-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Going low to reach high: Small-scale ChIP-seq maps new terrain.

    Fosslie, Madeleine / Manaf, Adeel / Lerdrup, Mads / Hansen, Klaus / Gilfillan, Gregor D / Dahl, John Arne

    Wiley interdisciplinary reviews. Systems biology and medicine

    2019  Volume 12, Issue 1, Page(s) e1465

    Abstract: Chromatin immunoprecipitation (ChIP) enables mapping of specific histone modifications or chromatin-associated factors in the genome and represents a powerful tool in the study of chromatin and genome regulation. Importantly, recent technological ... ...

    Abstract Chromatin immunoprecipitation (ChIP) enables mapping of specific histone modifications or chromatin-associated factors in the genome and represents a powerful tool in the study of chromatin and genome regulation. Importantly, recent technological advances that couple ChIP with whole-genome high-throughput sequencing (ChIP-seq) now allow the mapping of chromatin factors throughout the genome. However, the requirement for large amounts of ChIP-seq input material has long made it challenging to assess chromatin profiles of cell types only available in limited numbers. For many cell types, it is not feasible to reach high numbers when collecting them as homogeneous cell populations in vivo. Nonetheless, it is an advantage to work with pure cell populations to reach robust biological conclusions. Here, we review (a) how ChIP protocols have been scaled down for use with as little as a few hundred cells; (b) which considerations to be aware of when preparing small-scale ChIP-seq and analyzing data; and (c) the potential of small-scale ChIP-seq datasets for elucidating chromatin dynamics in various biological systems, including some examples such as oocyte maturation and preimplantation embryo development. This article is categorized under: Laboratory Methods and Technologies > Genetic/Genomic Methods Developmental Biology > Developmental Processes in Health and Disease Biological Mechanisms > Cell Fates.
    Language English
    Publishing date 2019-09-03
    Publishing country United States
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2503323-2
    ISSN 1939-005X ; 1939-5094
    ISSN (online) 1939-005X
    ISSN 1939-5094
    DOI 10.1002/wsbm.1465
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6790: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Histone Methylations Define Neural Stem/Progenitor Cell Subtypes in the Mouse Subventricular Zone.

    Zhang, Zhichao / Manaf, Adeel / Li, Yanjiao / Perez, Sonia Peña / Suganthan, Rajikala / Dahl, John Arne / Bjørås, Magnar / Klungland, Arne

    Molecular neurobiology

    2019  Volume 57, Issue 2, Page(s) 997–1008

    Abstract: Neural stem/progenitor cells (NSPCs) persist in the mammalian brain throughout life and can be activated in response to the physiological and pathophysiological stimuli. Epigenetic reprogramming of NPSC represents a novel strategy for enhancing the ... ...

    Abstract Neural stem/progenitor cells (NSPCs) persist in the mammalian brain throughout life and can be activated in response to the physiological and pathophysiological stimuli. Epigenetic reprogramming of NPSC represents a novel strategy for enhancing the intrinsic potential of the brain to regenerate after brain injury. Therefore, defining the epigenetic features of NSPCs is important for developing epigenetic therapies for targeted reprogramming of NSPCs to rescue neurologic function after injury. In this study, we aimed at defining different subtypes of NSPCs by individual histone methylations. We found the three histone marks, histone H3 lysine 4 trimethylation (H3K4me3), histone H3 lysine 27 trimethylation (H3K27me3), and histone H3 lysine 36 trimethylation (H3K36me3), to nicely and dynamically portray individual cell types during neurodevelopment. First, we found all three marks co-stained with NSPC marker SOX2 in mouse subventricular zone. Then, CD133, Id1, Mash1, and DCX immunostaining were used to define NSPC subtypes. Type E/B, B/C, and C/A cells showed high levels of H3K27me3, H3K36me3, and H3K4me3, respectively. Our results reveal defined histone methylations of NSPC subtypes supporting that epigenetic regulation is critical for neurogenesis and for maintaining NSPCs.
    MeSH term(s) Animals ; Epigenesis, Genetic/genetics ; Histones/metabolism ; Lateral Ventricles/metabolism ; Lysine/metabolism ; Methylation ; Mice, Inbred C57BL ; Neural Stem Cells/metabolism ; Neurogenesis/physiology ; Protein Processing, Post-Translational/physiology ; Regeneration/physiology ; Stem Cells/cytology
    Chemical Substances Histones ; Lysine (K3Z4F929H6)
    Language English
    Publishing date 2019-10-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 645020-9
    ISSN 1559-1182 ; 0893-7648
    ISSN (online) 1559-1182
    ISSN 0893-7648
    DOI 10.1007/s12035-019-01777-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2411: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: KDM4A regulates the maternal-to-zygotic transition by protecting broad H3K4me3 domains from H3K9me3 invasion in oocytes.

    Sankar, Aditya / Lerdrup, Mads / Manaf, Adeel / Johansen, Jens Vilstrup / Gonzalez, Javier Martin / Borup, Rehannah / Blanshard, Robert / Klungland, Arne / Hansen, Klaus / Andersen, Claus Yding / Dahl, John Arne / Helin, Kristian / Hoffmann, Eva R

    Nature cell biology

    2020  Volume 22, Issue 4, Page(s) 380–388

    Abstract: The importance of germline-inherited post-translational histone modifications on priming early mammalian development is just ... ...

    Abstract The importance of germline-inherited post-translational histone modifications on priming early mammalian development is just emerging
    MeSH term(s) Animals ; Embryo Implantation ; Embryo, Mammalian ; Female ; Fertilization/genetics ; Heterochromatin/chemistry ; Heterochromatin/metabolism ; Histone Demethylases/genetics ; Histone Demethylases/metabolism ; Histones/genetics ; Histones/metabolism ; Male ; Metaphase ; Methylation ; Mice ; Mice, Knockout ; Oocytes/cytology ; Oocytes/growth & development ; Oocytes/metabolism ; Promoter Regions, Genetic ; Protein Processing, Post-Translational ; Transcription, Genetic ; Zygote/cytology ; Zygote/growth & development ; Zygote/metabolism
    Chemical Substances Heterochromatin ; Histones ; histone H3 trimethyl Lys4 ; Histone Demethylases (EC 1.14.11.-) ; JMJD2A protein, mouse (EC 1.14.11.-)
    Language English
    Publishing date 2020-03-30
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1474722-4
    ISSN 1476-4679 ; 1465-7392
    ISSN (online) 1476-4679
    ISSN 1465-7392
    DOI 10.1038/s41556-020-0494-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 5169: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 12: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: 5-hydroxymethylcytosine Marks Mammalian Origins Acting as a Barrier to Replication.

    Prikrylova, Terezia / Robertson, Julia / Ferrucci, Francesca / Konorska, Dorota / Aanes, Håvard / Manaf, Adeel / Zhang, Beibei / Vågbø, Cathrine Broberg / Kuśnierczyk, Anna / Gilljam, Karin M / Løvkvam-Køster, Caroline / Otterlei, Marit / Dahl, John Arne / Enserink, Jorrit / Klungland, Arne / Robertson, Adam B

    Scientific reports

    2019  Volume 9, Issue 1, Page(s) 11065

    Abstract: In most mammalian cells, DNA replication occurs once, and only once between cell divisions. Replication initiation is a highly regulated process with redundant mechanisms that prevent errant initiation events. In lower eukaryotes, replication is ... ...

    Abstract In most mammalian cells, DNA replication occurs once, and only once between cell divisions. Replication initiation is a highly regulated process with redundant mechanisms that prevent errant initiation events. In lower eukaryotes, replication is initiated from a defined consensus sequence, whereas a consensus sequence delineating mammalian origin of replication has not been identified. Here we show that 5-hydroxymethylcytosine (5hmC) is present at mammalian replication origins. Our data support the hypothesis that 5hmC has a role in cell cycle regulation. We show that 5hmC level is inversely proportional to proliferation; indeed, 5hmC negatively influences cell division by increasing the time a cell resides in G1. Our data suggest that 5hmC recruits replication-licensing factors, then is removed prior to or during origin firing. Later we propose that TET2, the enzyme catalyzing 5mC to 5hmC conversion, acts as barrier to rereplication. In a broader context, our results significantly advance the understating of 5hmC involvement in cell proliferation and disease states.
    MeSH term(s) 5-Methylcytosine/analogs & derivatives ; 5-Methylcytosine/metabolism ; Cell Cycle/genetics ; Cell Division/physiology ; Cell Proliferation/physiology ; DNA Replication/physiology ; HeLa Cells ; Humans ; Replication Origin
    Chemical Substances 5-hydroxymethylcytosine (1123-95-1) ; 5-Methylcytosine (6R795CQT4H)
    Language English
    Publishing date 2019-07-30
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-019-47528-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article ; Online: Broad histone H3K4me3 domains in mouse oocytes modulate maternal-to-zygotic transition.

    Dahl, John Arne / Jung, Inkyung / Aanes, Håvard / Greggains, Gareth D / Manaf, Adeel / Lerdrup, Mads / Li, Guoqiang / Kuan, Samantha / Li, Bin / Lee, Ah Young / Preissl, Sebastian / Jermstad, Ingunn / Haugen, Mads Haugland / Suganthan, Rajikala / Bjørås, Magnar / Hansen, Klaus / Dalen, Knut Tomas / Fedorcsak, Peter / Ren, Bing /
    Klungland, Arne

    Nature

    2016  Volume 537, Issue 7621, Page(s) 548–552

    Abstract: Maternal-to-zygotic transition (MZT) is essential for the formation of a new individual, but is still poorly understood despite recent progress in analysis of gene expression and DNA methylation in early embryogenesis. Dynamic histone modifications may ... ...

    Abstract Maternal-to-zygotic transition (MZT) is essential for the formation of a new individual, but is still poorly understood despite recent progress in analysis of gene expression and DNA methylation in early embryogenesis. Dynamic histone modifications may have important roles in MZT, but direct measurements of chromatin states have been hindered by technical difficulties in profiling histone modifications from small quantities of cells. Recent improvements allow for 500 cell-equivalents of chromatin per reaction, but require 10,000 cells for initial steps or require a highly specialized microfluidics device that is not readily available. We developed a micro-scale chromatin immunoprecipitation and sequencing (μChIP-seq) method, which we used to profile genome-wide histone H3 lysine methylation (H3K4me3) and acetylation (H3K27ac) in mouse immature and metaphase II oocytes and in 2-cell and 8-cell embryos. Notably, we show that ~22% of the oocyte genome is associated with broad H3K4me3 domains that are anti-correlated with DNA methylation. The H3K4me3 signal becomes confined to transcriptional-start-site regions in 2-cell embryos, concomitant with the onset of major zygotic genome activation. Active removal of broad H3K4me3 domains by the lysine demethylases KDM5A and KDM5B is required for normal zygotic genome activation and is essential for early embryo development. Our results provide insight into the onset of the developmental program in mouse embryos and demonstrate a role for broad H3K4me3 domains in MZT.
    MeSH term(s) Acetylation ; Animals ; Cell Line, Tumor ; Chromatin/genetics ; Chromatin/metabolism ; Chromatin Immunoprecipitation ; DNA Methylation ; Embryonic Development/genetics ; Female ; Gene Expression Regulation, Developmental ; Genome/genetics ; Histones/chemistry ; Histones/metabolism ; Humans ; Lysine/metabolism ; Male ; Methylation ; Mice ; Oocytes/metabolism ; Sequence Analysis, DNA ; Transcription Initiation Site ; Zygote/cytology ; Zygote/metabolism
    Chemical Substances Chromatin ; Histones ; Lysine (K3Z4F929H6)
    Language English
    Publishing date 2016-09-14
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 120714-3
    ISSN 1476-4687 ; 0028-0836
    ISSN (online) 1476-4687
    ISSN 0028-0836
    DOI 10.1038/nature19360
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 26: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 9: Show issues
    Zs.MO 244: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top