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  1. Article ; Online: Characterization and in-vitro Alzheimer’s properties of exopolysaccharide from Bacillus maritimus MSM1

    Manal S. Selim / Sahar S. Mohamed / Mohsen S. Asker / Abeer Y. Ibrahim / Samah A. El-Newary / Mohamed E. El Awady

    Scientific Reports, Vol 13, Iss 1, Pp 1-

    2023  Volume 17

    Abstract: Abstract Four bacterial isolates were obtained from marine sediments collected from Sahl Hashish, Hurghada Red Sea, Egypt. This study was designed to search for promising anti-Alzheimer natural polysaccharide; therefore, four isolates were screened for ... ...

    Abstract Abstract Four bacterial isolates were obtained from marine sediments collected from Sahl Hashish, Hurghada Red Sea, Egypt. This study was designed to search for promising anti-Alzheimer natural polysaccharide; therefore, four isolates were screened for exopolysaccharides (EPSs) production and acetylcholinesterase inhibition. The isolate S16 provided the highest EPS yield (7.51 g/L) and acetylcholinesterase inhibition. It was identified morphologically and genetically using 16S rRNA gene sequence analysis as Bacillus maritimus. A Physicochemical analysis of S16 exopolysaccharide (BMEPS) was estimated, which pointed to the presence of uronic acid and sulfate (24.7% and 18.3%, respectively). HPLC analysis indicated that mannuronic acid, glucuronic acid, glucose, and mannose are presented in a molar ratio of 0.8:1.0:2.8:2.3, respectively. Furthermore, FT-IR revealed an abundance of β-configurations. The GPC estimated the average molecular weight (Mw) as 4.31 × 104 g/mol. BMEPS inhibited AChE (IC50; 691.77 ± 8.65 μg/ ml), BChE (IC50; 288.27 ± 10.50 μg/ ml), and tyrosinase (IC50; 3.34 ± 0.09, 14.00 ± 0.14, and 22.96 ± 1.23 μg/ ml during incubation durations of 10, 20, and 40 min). It also demonstrated a selective anti-inflammatory action against COX-2 rather than COX-1. Moreover, BMEPS exhibited antioxidant capabilities as free radical and oxygen reactive species (ROS) scavenger, metal chelator, reductant agent, and lipid peroxidation suppressor. These activities are due to the distinct chemical composition. The findings of this study indicate that BMEPS could be considered as promising anti-disease Alzheimer's (AD) material in an in-vitro model, which qualifies it for advanced in-vivo studies in the discovery of alternative Alzheimer’s treatment.
    Keywords Medicine ; R ; Science ; Q
    Subject code 540
    Language English
    Publishing date 2023-07-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Medical application of exopolymers produced by marine bacteria

    Sayeda A. Abdelhamid / Sahar S. Mohamed / Manal S. Selim

    Bulletin of the National Research Centre, Vol 44, Iss 1, Pp 1-

    2020  Volume 14

    Abstract: Abstract Background Natural products have been viewed as essential sources that could create potential chemotherapeutic agents. In the look for new bioactive substances, examinations were extended to marine territories. Results Humanity has known for the ...

    Abstract Abstract Background Natural products have been viewed as essential sources that could create potential chemotherapeutic agents. In the look for new bioactive substances, examinations were extended to marine territories. Results Humanity has known for the last few thousand years that a marine organism contains substances fit for strong biological activity. However, the main genuine examination of marine living beings began just 50 years prior. Since then, all types of life in the marine condition (e.g., bacteria, algae, and fungi) have been researched for their bioactive content. Conclusions Exopolymers can be applied in a wide range of industrial applications in biomedicines.
    Keywords Exopolymers ; Marine bacteria ; Bioactive substances ; Science ; Q
    Language English
    Publishing date 2020-05-01T00:00:00Z
    Publisher SpringerOpen
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Production of exopolysaccharides from novel marine bacteria and anticancer activity against hepatocellular carcinoma cells (HepG2)

    Mohsen S. Asker / Osama H. El Sayed / Manal G. Mahmoud / Shaymaa M. Yahya / Sahar S. Mohamed / Manal S. Selim / Mohamed S. El Awady / Salma M. Abdelnasser / Mostafa M. Abo Elsoud

    Bulletin of the National Research Centre, Vol 42, Iss 1, Pp 1-

    2018  Volume 9

    Abstract: Abstract Background The aim of the current study based on the production and characterization of exopolysaccharides (EPSs) isolated from marine sediment of the Mediterranean and Red Seas is to study its cytotoxic activity against HepG2 cells. Results ... ...

    Abstract Abstract Background The aim of the current study based on the production and characterization of exopolysaccharides (EPSs) isolated from marine sediment of the Mediterranean and Red Seas is to study its cytotoxic activity against HepG2 cells. Results Eleven isolates have the ability to produce EPSs and also decreased the viability of HepG2 cell line in different manners. The five most promising isolates that produce high yield of EPSs and high cytotoxicity were identified by 16S RNA as Brevundimonas subvibrioides MSA1, Bacillus thuringiensis E4, Bacillus amyloliquefaciens MGA2, Pseudomonas fluorescens SGA3, and Advenella Kashmirensis NRC-7. The chemical composition of the following EPSs (M1, M3, M6, M15, M19, E2, E4, E10, S5, S7, and S11) demonstrates that they are acidic sulfated heteropolysaccharides with different relative ratios of monosugars of glucose, mannose, galactose, glucouronic acid, and mannouronic acid. The average molecular weights from 1.94 × 104 to 7.95 × 105 g/mol and the number average molecular weight from 1.51 × 104 to 7.53 × 105 g/mol. FTIR spectrum of the five EPSs indicated the presence of sulfate and carboxylic groups in different percentages. Conclusions The EPSs produced from marine bacteria are very promising for treating the HepG2 cells.
    Keywords Exopolysaccharides ; Marine bacteria ; Cytotoxicity ; Hepatocellular carcinoma cells ; Science ; Q
    Language English
    Publishing date 2018-12-01T00:00:00Z
    Publisher SpringerOpen
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Protective effect of some natural products against chemotherapy-induced toxicity in rats

    Heba M.I. Abdallah / Rehab F. Abdel-Rahman / Sally A. El Awdan / Rasha M. Allam / Aliaa E.M.K. El-Mosallamy / Manal S. Selim / Sahar S. Mohamed / Mahmoud S. Arbid / Abdel Razik H. Farrag

    Heliyon, Vol 5, Iss 5, Pp e01590- (2019)

    2019  

    Abstract: Aim: There is a great interest in combining anticancer drugs with natural products aiming at maximizing their efficacy while minimizing systemic toxicity. Hence, the present study was constructed aiming to investigate the protective potential of three ... ...

    Abstract Aim: There is a great interest in combining anticancer drugs with natural products aiming at maximizing their efficacy while minimizing systemic toxicity. Hence, the present study was constructed aiming to investigate the protective potential of three natural products, 1,8-cineole an essential oil from Artemisia herba alba, exopolysaccharide (EPS) from locally identified marine streptomycete, and ellagic acid (EA), against chemotherapy-induced organ toxicity. Methods: Isolation, production and characterization of EPS from marine streptomycete was done. Animals were allocated into five groups, GP1: normal control, GP2: cyclophosphamide (CYC), GP3: 1,8-cineole + CYC, GP4: EPS + CYC, GP4: EA + CYC. All drugs were administered orally 1 week before and concomitantly with CYC. Electrocardiography (ECG) analysis, liver enzymes (ALT and AST), cardiac serum markers (LDH and CK), oxidative stress biomarkers in hepatic and cardiac tissues (GSH and MDA), TGF-β1 and histopathological examination of hepatic and cardiac tissues were executed. Results: The isolated stain produced EPS was identified as Streptomyces xiamenensis. EPS contains uronic, sulphate groups and different monosugars with Mw 4.65 × 104 g/mol and showed antioxidant activity against DPPH. Pretreatment of rats with 1,8-cineole, EPS and EA improved ECG abnormalities, decrease serum markers of hepato- and cardiotoxicity, prevent oxidative stress and decrease TGF-β1 in liver and heart tissues. Conclusion: The present results demonstrate the hepatoprotective and cardioprotective effects of the above-mentioned natural products against CYC organ toxicity.
    Keywords Biochemistry ; Cancer research ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
    Subject code 630
    Language English
    Publishing date 2019-05-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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