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  1. Article ; Online: BALF and BLOOD NK- cells in different stages of pulmonary sarcoidosis.

    Manika, Katerina / Domvri, Kalliopi / Kyriazis, George / Kontakiotis, Theodoros / Papakosta, Despina

    Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG

    2022  Volume 38, Issue 4, Page(s) e2021039

    Abstract: Background and objective: Data on natural killer (NK)- and natural killer T (NKT)- like cells in the immunopathogenesis of sarcoidosis remain limited. The aim was to assess NK- and NKT-like cells across different stages in bronchoalveolar lavage (BALF) ... ...

    Abstract Background and objective: Data on natural killer (NK)- and natural killer T (NKT)- like cells in the immunopathogenesis of sarcoidosis remain limited. The aim was to assess NK- and NKT-like cells across different stages in bronchoalveolar lavage (BALF) versus peripheral blood (PB) in comparison to controls.
    Methods: Forty four patients (32 women and 12 men, mean age 46.6±14.4 years) with biopsy-proven sarcoidosis and 10 healthy individuals (6 women, 4 men mean age 52.6±19.1 years) were submitted to BALF. Total cells and cell differentials were counted, while CD45+, CD3+, CD4+, CD8+, CD19+, CD3-CD16/56 (NK cells) and CD3+CD16/56+ (NKT-like cells) were determined by dual flow cytometry in BALF and PB.
    Results: A significantly lower percentage of both NK and NKT-like cells was observed in BALF of controls and sarcoid patients (SP) compared to PB. Both BALF NK and NKT-cell counts were significantly higher in SP than in controls (NK: p=0.046, NKT-like: p=0.012) In addition BALF NK cell percentage differed among sarcoidosis stages (p=0.005). In PB NK-cell count was lower in sarcoidosis patients but the difference did not reach statistical significance. Also, in sarcoid patients' BALF NK-cell percentage negatively correlated with lymphocyte percentage (r=-0.962, p<0.001).
    Conclusions: The increased count of BALF NK and NKT-like cells in sarcoidosis compared to controls along with the increase of NK cells with stage progression are in line with a growing number of investigations suggesting the involvement of NK- and NKT-like cells in the pathogenesis of sarcoidosis.
    Language English
    Publishing date 2022-01-13
    Publishing country Italy
    Document type Journal Article
    ZDB-ID 1339192-6
    ISSN 2532-179X ; 1124-0490
    ISSN (online) 2532-179X
    ISSN 1124-0490
    DOI 10.36141/svdld.v38i4.10810
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  2. Article ; Online: Occupational Exposure to Zoonotic Tuberculosis Caused by Mycobacterium caprae, Northern Greece, 2019.

    Papaventsis, Dimitrios / Dougas, George / Kalkouni, Ourania / Karabela, Simona / Manika, Katerina

    Emerging infectious diseases

    2021  Volume 27, Issue 7, Page(s) 1997–1999

    Abstract: Pulmonary tuberculosis caused by Mycobacterium caprae was diagnosed in a 65-year-old goat breeder from northern Greece. This case represents a documented occupational transmission of M. caprae and highlights the importance of enhanced laboratory ... ...

    Abstract Pulmonary tuberculosis caused by Mycobacterium caprae was diagnosed in a 65-year-old goat breeder from northern Greece. This case represents a documented occupational transmission of M. caprae and highlights the importance of enhanced laboratory screening and increased surveillance for zoonotic tuberculosis control.
    MeSH term(s) Aged ; Farmers ; Greece ; Humans ; Male ; Mycobacterium bovis ; Mycobacterium tuberculosis ; Occupational Exposure ; Tuberculosis
    Language English
    Publishing date 2021-06-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1380686-5
    ISSN 1080-6059 ; 1080-6040
    ISSN (online) 1080-6059
    ISSN 1080-6040
    DOI 10.3201/eid2707.204399
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  3. Article: Clinical, imaging and functional determinants of sarcoidosis phenotypes in a Greek population.

    Spyropoulos, Georgios / Domvri, Kalliopi / Manika, Katerina / Fouka, Evangelia / Kontakiotis, Theodoros / Papakosta, Despoina

    Journal of thoracic disease

    2022  Volume 14, Issue 6, Page(s) 1941–1949

    Abstract: Background: The aim of the present study was the application of the latest phenotype recommendations in Greek patients, in order to identify specific clinical, imaging and spirometric characteristics, at initial diagnosis of sarcoidosis, related to ... ...

    Abstract Background: The aim of the present study was the application of the latest phenotype recommendations in Greek patients, in order to identify specific clinical, imaging and spirometric characteristics, at initial diagnosis of sarcoidosis, related to disease phenotypes.
    Methods: Our cohort included 147 patients coming from Northern Greece, recruited from the Outpatient Sarcoidosis Clinic, of Aristotle University of Thessaloniki. The observation period was 5 years. The Scadding staging system and the World Association of Sarcoidosis and other Granulomatous Disorders (WASOG) Clinical Outcome Status instrument were used. Phenotypes were defined by the latest DELPHI consensus recommendations.
    Results: The following clinical phenotypes were identified: asymptomatic 59%, acute 14.3%, chronic 12.9% and advanced 33.3%. The observed phenotypes were not related to Scadding stages. Lung function decline was in line with phenotype severity. The presence of fibrosis to any extent upon diagnosis differed among phenotypes (asymptomatic 13.8%, acute 38.1%, chronic 57.9%, advanced 61.2%, P<0.001) and was common for relapsing patients (P<0.001). In spontaneously remitting patients, fibrosis upon diagnosis was found less often than in non-remitting patients (P<0.001). Renal involvement was more frequently found in the advanced phenotype (P=0.032). Skin involvement was more common for patients with acute onset (P<0.001) and spontaneous remission (P=0.012). Ocular involvement was mainly found in relapsing patients (P<0.001).
    Conclusions: In our cohort, sarcoidosis clinical phenotypes have certain clinical, imaging and functional characteristics, at initial diagnosis of the disease, which could be assessed in everyday practice.
    Language English
    Publishing date 2022-05-16
    Publishing country China
    Document type Journal Article
    ZDB-ID 2573571-8
    ISSN 2077-6624 ; 2072-1439
    ISSN (online) 2077-6624
    ISSN 2072-1439
    DOI 10.21037/jtd-21-1760
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  4. Article: Tigecycline Pharmacokinetic and Pharmacodynamic Profile in Patients with Chronic Obstructive Pulmonary Disease Exacerbation.

    Kipourou, Maria / Begou, Olga / Manika, Katerina / Ismailos, Georgios / Kontou, Paschalina / Pitsiou, Georgia / Gika, Helen / Kioumis, Ioannis

    Antibiotics (Basel, Switzerland)

    2023  Volume 12, Issue 2

    Abstract: Background: We aimed to evaluate the pharmacokinetic profile of tigecycline in plasma and its penetration to sputum in moderately ill patients with an infectious acute exacerbation of chronic obstructive pulmonary disease (COPD).: Methods: Eleven ... ...

    Abstract Background: We aimed to evaluate the pharmacokinetic profile of tigecycline in plasma and its penetration to sputum in moderately ill patients with an infectious acute exacerbation of chronic obstructive pulmonary disease (COPD).
    Methods: Eleven patients hospitalized with acute respiratory failure due to an acute COPD exacerbation with clinical evidence of an infectious cause received tigecycline 50 mg twice daily after an initial loading dose of 100 mg. Blood and sputum samples were collected at steady state after dose seven.
    Results: In plasma, mean C
    Conclusions: Our findings support the clinical effectiveness of tigecycline against commonly causative bacteria in COPD exacerbations and highlight its sufficient lung penetration in pulmonary infections of moderate severity.
    Language English
    Publishing date 2023-02-02
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2681345-2
    ISSN 2079-6382
    ISSN 2079-6382
    DOI 10.3390/antibiotics12020307
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  5. Article ; Online: Severe Pulmonary Disease Caused by Mycolicibacter kumamotonensis.

    Manika, Katerina / Kontos, Fanourios / Papavasileiou, Apostolos / Papaventsis, Dimitrios / Sionidou, Maria / Kioumis, Ioannis

    Emerging infectious diseases

    2021  Volume 27, Issue 3, Page(s) 962–964

    Abstract: Severe Mycolicibacter kumamotonensis-pulmonary disease was diagnosed in a 68-year-old immunocompetent woman in Greece; the disease was initially treated as tuberculosis. The patient responded favorably to a new treatment regimen of azithromycin, amikacin, ...

    Abstract Severe Mycolicibacter kumamotonensis-pulmonary disease was diagnosed in a 68-year-old immunocompetent woman in Greece; the disease was initially treated as tuberculosis. The patient responded favorably to a new treatment regimen of azithromycin, amikacin, moxifloxacin, and linezolid. Complete symptom resolution and radiologic improvement resulted.
    MeSH term(s) Aged ; Anti-Bacterial Agents ; Female ; Greece ; Humans ; Lung Diseases ; Mycobacteriaceae ; Mycobacterium Infections, Nontuberculous ; Nontuberculous Mycobacteria
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2021-02-23
    Publishing country United States
    Document type Case Reports ; Journal Article
    ZDB-ID 1380686-5
    ISSN 1080-6059 ; 1080-6040
    ISSN (online) 1080-6059
    ISSN 1080-6040
    DOI 10.3201/eid2703.191648
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    Zs.A 4778: Show issues Location:
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  6. Article ; Online: Adherence to inhaled therapies over 4 years in people with cystic fibrosis.

    Manika, Katerina / Hatziagorou, Elpis / Kotoulas, Serafeim-Chrysovalantis / Kyrvasili, Syrmo-Styliani / Sourla, Evdokia / Kouroukli, Eleana / Sionidou, Maria / Papadaki, Eleni / Tsanakas, John

    Pediatric pulmonology

    2022  Volume 57, Issue 4, Page(s) 956–964

    Abstract: Background: This study examined the drug-specific and overall adherence of teenagers and adults with cystic fibrosis (CF) to inhaled therapies, to assess the degree of adherence, stability over a period of 4 years, and its association with health ... ...

    Abstract Background: This study examined the drug-specific and overall adherence of teenagers and adults with cystic fibrosis (CF) to inhaled therapies, to assess the degree of adherence, stability over a period of 4 years, and its association with health outcomes.
    Methods: Fifty-five participants (30 women and 25 men) aged 14 years or older from two CF centers were enrolled in a retrospective review of inhaled medication adherence over 4 years. Adherence was assessed by the number of doses that were obtained by each participant based on the "e-prescription.gr" platform and the calculation of the medication possession ratio (MPR).
    Results: The mean composite MPR (cMPR) for the entire research period was 0.75 ± 0.19. A total of 43.4% of participants showed a variance of adherence <25%. Participants with stable adherence had a significantly higher mean cMPR compared with those with variable adherence (0.86 ± 0.16 vs. 0.66 ± 0.17, p < 0.001). A statistically significant difference between groups of patients with different degrees of mean cMPR and mean weight was observed (p = 0.011). Patients with a mean cMPR ≥0.80 weighed significantly more than those with moderate and low adherence. In addition, mean weight correlated significantly with the mean cMPR (Β [95% confidence interval] = 14.845 [0.191-29.498], r = 0.269, p = 0.047).
    Conclusions: In our setting, the cMPR was easy to assess and showed that adherence was probably better than expected. The association of cMPR with weight should be further investigated. Stable adherence seemed to be related to high adherence. This observation could enhance our understanding of people with CF and their approach to treatment.
    MeSH term(s) Adolescent ; Adult ; Cystic Fibrosis/drug therapy ; Female ; Humans ; Male ; Medication Adherence ; Retrospective Studies
    Language English
    Publishing date 2022-01-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 632784-9
    ISSN 1099-0496 ; 8755-6863
    ISSN (online) 1099-0496
    ISSN 8755-6863
    DOI 10.1002/ppul.25834
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  7. Article ; Online: Evaluation of Pain Scales and Outcome in Critically Ill Patients of a Greek ICU.

    Kontou, Paschalina / Kotoulas, Serafeim-Chrysovalantis / Kalliontzis, Stavros / Synodinos-Kamilos, Spyridon / Akritidou, Sofia / Kaimakamis, Evangelos / Anisoglou, Souzana / Manika, Katerina

    Journal of pain & palliative care pharmacotherapy

    2022  Volume 37, Issue 1, Page(s) 34–43

    Abstract: The purpose of the study was to evaluate painful procedures in ICU patients and to investigate their effect as well as the role of analgesia in the outcome. We measured pain level and vital signs before, during and after potentially painful procedures by ...

    Abstract The purpose of the study was to evaluate painful procedures in ICU patients and to investigate their effect as well as the role of analgesia in the outcome. We measured pain level and vital signs before, during and after potentially painful procedures by using the Behavioral Pain Scale (BPS) and the Critical Care Pain Observation Tool (CPOT). We analyzed the correlation of these measurements and of analgesia with the outcome. Twenty-eight patients were subjected to 160 stimuli. There were statistically significant differences in pain scores and most vital signs between the different timepoints (before-during, during-after). Most of them were significantly correlated with each other. Physiotherapy proved to be the most painful procedure. Regarding the outcome, the administration of extra analgesia predicted less days of mechanical ventilation (
    MeSH term(s) Humans ; Critical Illness ; Greece ; Reproducibility of Results ; Critical Care/methods ; Pain ; Intensive Care Units
    Language English
    Publishing date 2022-12-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 2078852-6
    ISSN 1536-0539 ; 1536-0288
    ISSN (online) 1536-0539
    ISSN 1536-0288
    DOI 10.1080/15360288.2022.2149668
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  8. Article ; Online: Pulmonary medication adherence among children and adults with cystic fibrosis: Is there an association with disease severity?

    Hatziagorou, Elpis / Manika, Katerina / Kyrvasili, Syrmo Styliani / Kotoulas, Serafeim Chrysovalantis / Chrysochoou, Elisavet Anna / Kouroukli, Eleana / Sionidou, Maria / Tsanakas, John

    Pediatric pulmonology

    2022  Volume 57, Issue 12, Page(s) 3017–3026

    Abstract: Background: Adherence to pulmonary medication is pivotal in delaying the progression of lung disease in cystic fibrosis (CF). Further exploring the consequences of poor adherence and its impact on disease severity may be valuable to personalize CF ... ...

    Abstract Background: Adherence to pulmonary medication is pivotal in delaying the progression of lung disease in cystic fibrosis (CF). Further exploring the consequences of poor adherence and its impact on disease severity may be valuable to personalize CF treatment strategy.
    Aim: To evaluate indicators of disease severity among children and adults with CF and investigate which of them are related to pulmonary medication adherence.
    Methods: This is a retrospective cohort study. Data of children and adults followed up in one pediatric and one adult CF Unit were evaluated over 4 years. Disease severity was assessed by measuring body mass index (BMI), lung function, history of pulmonary exacerbations, and medication complexity. Adherence was assessed by calculating a 12-month medication possession ratio (MPR) for each pulmonary medication and then averaged for a composite MPR (cMPR) for each patient. Regression analysis was performed to explore the association of adherence with disease severity.
    Results: Ninety-five patients were included in our study, 52 children and 43 adults. The overall cMPR was 0.74 (SD = 0.25); 0.68 (SD = 0.24) for children and 0.81 (SD = 0.24) for adults. Adults presented higher adherence, with overall mean cMPR 0.81 (SD = 0.24) compared to children with mean cMPR 0.68 (SD = 0.24) (p < 0.05, 95% CI = -0.27 to -0.03). Adherence was inversely related to FEV
    Conclusion: An overall moderate to high level of adherence was found among our study population. Adults presented higher adherence compared to children. FEV1% and FVC% predicted were related to a significant decrease in adherence. Among our group of CF patients with an overall moderate to high level of adherence, adherence to pulmonary medication was inversely related to disease severity.
    MeSH term(s) Adult ; Humans ; Child ; Cystic Fibrosis/drug therapy ; Retrospective Studies ; Medication Adherence ; Lung ; Severity of Illness Index
    Language English
    Publishing date 2022-09-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 632784-9
    ISSN 1099-0496 ; 8755-6863
    ISSN (online) 1099-0496
    ISSN 8755-6863
    DOI 10.1002/ppul.26126
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  9. Article: 18F-FDG PET/CT contribution to tuberculous vertebral osteomyelitis diagnosis: a case report.

    Manika, Katerina / Kipourou, Maria / Georga, Stamata / Faniadou, Eleni / Pilianidis, Georgios / Arsos, Georgios / Kioumis, Ioannis

    Oxford medical case reports

    2020  Volume 2020, Issue 9, Page(s) omaa068

    Abstract: Tuberculous vertebral osteomyelitis (TVO) is an extrapulmonary tuberculosis form characterized by difficulty and delay in diagnosis. PET/CT is a valuable, well-established tool in the diagnostic workup of cancer and fever of unknown origin, which is ... ...

    Abstract Tuberculous vertebral osteomyelitis (TVO) is an extrapulmonary tuberculosis form characterized by difficulty and delay in diagnosis. PET/CT is a valuable, well-established tool in the diagnostic workup of cancer and fever of unknown origin, which is increasingly appreciated in the management of infectious diseases. We report a TVO case where PET/CT had a valuable contribution towards diagnosis and monitoring of treatment response, highlighting its advantages and future perspectives when dealing with infectious diseases.
    Language English
    Publishing date 2020-09-22
    Publishing country England
    Document type Case Reports
    ZDB-ID 2766251-2
    ISSN 2053-8855
    ISSN 2053-8855
    DOI 10.1093/omcr/omaa068
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  10. Article ; Online: Use of cystic fibrosis inhaled medication before and after elexacaftor/tezacaftor/ivacaftor initiation.

    Manika, Katerina / Diamantea, Filia / Tsakona, Anna / Kakolyris, Alexandros / Sopiadou, Athina / Kotoulas, Serafeim-Chrysovalantis / Sionidou, Maria / Kirvasili, Sirmo-Stiliani / Hadji-Mitrova, Marija / Papadaki, Eleni / Chrysochoou, Elisavet-Anna / Hatziagorou, Elpis

    Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society

    2023  Volume 23, Issue 1, Page(s) 29–31

    Abstract: Elexacator/tezacaftor/ivacaftor (ETI) has improved cystic fibrosis (CF) outcomes. A reduction in use of maintenance medication after its initiation has been reported. Seventy-one adult people with CF (PwCF) who are followed in three CF centers and ... ...

    Abstract Elexacator/tezacaftor/ivacaftor (ETI) has improved cystic fibrosis (CF) outcomes. A reduction in use of maintenance medication after its initiation has been reported. Seventy-one adult people with CF (PwCF) who are followed in three CF centers and completed one year of treatment with ETI were included in this study. Their use of inhaled dornase-α, colistin, tobramycin, aztreonam and levofloxacin during this period was compared with the corresponding use during one year without ETI, using the Medication Possession Ratio (MPR). MPR was significantly decreased after ETI initiation for dornase-α (67±35% vs 48±40%, p<0.001) and for all four inhaled antibiotics together (62±33% vs 41±37%, p<0.001). The findings of this multi-center, retrospective, study suggest that the initiation of ETI significantly leads to decrease in use of standard inhaled medication in PwCF. The significance of this finding in the course of the disease is yet to be investigated by larger prospective clinical trials.
    MeSH term(s) Adult ; Humans ; Cystic Fibrosis/diagnosis ; Cystic Fibrosis/drug therapy ; Cystic Fibrosis/genetics ; Prospective Studies ; Retrospective Studies ; Cystic Fibrosis Transmembrane Conductance Regulator/genetics ; Mutation ; Benzodioxoles/adverse effects ; Aminophenols/adverse effects ; Indoles ; Pyrazoles ; Pyridines ; Pyrrolidines ; Quinolones
    Chemical Substances ivacaftor (1Y740ILL1Z) ; elexacaftor (RRN67GMB0V) ; tezacaftor ; Cystic Fibrosis Transmembrane Conductance Regulator (126880-72-6) ; Benzodioxoles ; Aminophenols ; Indoles ; Pyrazoles ; Pyridines ; Pyrrolidines ; Quinolones
    Language English
    Publishing date 2023-05-09
    Publishing country Netherlands
    Document type Multicenter Study ; Journal Article
    ZDB-ID 2084724-5
    ISSN 1873-5010 ; 1569-1993
    ISSN (online) 1873-5010
    ISSN 1569-1993
    DOI 10.1016/j.jcf.2023.05.001
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