Article ; Online: Quantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study, 2011-2018.
2019 Volume 9, Issue 1, Page(s) 16675
Abstract: Household contacts (HHC) of leprosy patients exhibit high-risk of developing leprosy and contact tracing is helpful for early diagnosis. From 2011 to 2018,2,437 HHC were examined in a clinic in Rio de Janeiro, Brazil and 16S qPCR was used for diagnosis ... ...
| Abstract | Household contacts (HHC) of leprosy patients exhibit high-risk of developing leprosy and contact tracing is helpful for early diagnosis. From 2011 to 2018,2,437 HHC were examined in a clinic in Rio de Janeiro, Brazil and 16S qPCR was used for diagnosis and monitoring of contacts. Fifty-four HHCs were clinically diagnosed with leprosy at intake. Another 25 exhibited leprosy-like skin lesions at intake, 8 of which were confirmed as having leprosy (50% of which were qPCR positive) and 17 of which were diagnosed with other skin diseases (6% qPCR positive). In skin biopsies, qPCR presented a sensitivity of 0.50 and specificity of 0.94. Furthermore, 955 healthy HHCs were followed-up for at least 3 years and skin scrapings were collected from earlobes for qPCR detection. Positive qPCR indicated a non-significant relative risk of 2.52 of developing the disease. During follow-up, those who progressed towards leprosy exhibited 20% qPCR positivity, compared to 9% of those who remained healthy. Disease-free survival rates indicated that age had a significant impact on disease progression, where patients over 60 had a greater chance of developing leprosy [HR = 32.4 (3.6-290.3)]. Contact tracing combined with qPCR may assist in early diagnosis and age is a risk factor for leprosy progression. |
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| MeSH term(s) | Adolescent ; Adult ; Brazil/epidemiology ; Case-Control Studies ; Child ; Child, Preschool ; Cohort Studies ; Contact Tracing/methods ; DNA, Bacterial/analysis ; DNA, Ribosomal/analysis ; Family Characteristics ; Female ; Follow-Up Studies ; Humans ; Infant ; Leprosy/diagnosis ; Leprosy/epidemiology ; Leprosy/genetics ; Male ; Middle Aged ; Molecular Diagnostic Techniques ; Mycobacterium leprae/genetics ; Mycobacterium leprae/isolation & purification ; Real-Time Polymerase Chain Reaction/methods ; Time Factors ; Young Adult |
| Chemical Substances | DNA, Bacterial ; DNA, Ribosomal |
| Language | English |
| Publishing date | 2019-11-13 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2615211-3 |
| ISSN | 2045-2322 ; 2045-2322 |
| ISSN (online) | 2045-2322 |
| ISSN | 2045-2322 |
| DOI | 10.1038/s41598-019-52640-5 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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