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  1. Article ; Online: Necroptosis Induced by Delta-Tocotrienol Overcomes Docetaxel Chemoresistance in Prostate Cancer Cells

    Marina Montagnani Marelli / Giangiacomo Beretta / Roberta Manuela Moretti

    International Journal of Molecular Sciences, Vol 24, Iss 4923, p

    2023  Volume 4923

    Abstract: Prostate cancer (PCa) represents the fifth cause of cancer death in men. Currently, chemotherapeutic agents for the treatment of cancers, including PCa, mainly inhibit tumor growth by apoptosis induction. However, defects in apoptotic cellular responses ... ...

    Abstract Prostate cancer (PCa) represents the fifth cause of cancer death in men. Currently, chemotherapeutic agents for the treatment of cancers, including PCa, mainly inhibit tumor growth by apoptosis induction. However, defects in apoptotic cellular responses frequently lead to drug resistance, which is the main cause of chemotherapy failure. For this reason, trigger non-apoptotic cell death might represent an alternative approach to prevent drug resistance in cancer. Several agents, including natural compounds, have been shown to induce necroptosis in human cancer cells. In this study we evaluated the involvement of necroptosis in anticancer activity of delta-tocotrienol (δ-TT) in PCa cells (DU145 and PC3). Combination therapy is one tool used to overcome therapeutic resistance and drug toxicity. Evaluating the combined effect of δ-TT and docetaxel (DTX), we found that δ-TT potentiates DTX cytotoxicity in DU145 cells. Moreover, δ-TT induces cell death in DU145 cells that have developed DTX resistance (DU-DXR) activating necroptosis. Taken together, obtained data indicate the ability of δ-TT to induce necroptosis in both DU145, PC3 and DU-DXR cell lines. Furthermore, the ability of δ-TT to induce necroptotic cell death may represent a promising therapeutical approach to overcome DTX chemoresistance in PCa.
    Keywords necroptosis ; delta-tocotrienol ; prostate cancer ; docetaxel ; chemoresistance ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 610
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Genealogical Data Mining from Historical Archives

    Angelica Lo Duca / Andrea Marchetti / Manuela Moretti / Francesca Diana / Mafalda Toniazzi / Andrea D’Errico

    Informatics, Vol 10, Iss 42, p

    The Case of the Jewish Community in Pisa

    2023  Volume 42

    Abstract: The Jewish community archive in Pisa owns a vast collection of documents and manuscripts that date back centuries. These documents contain valuable genealogical information, including birth, marriage, and death records. This paper aims to describe the ... ...

    Abstract The Jewish community archive in Pisa owns a vast collection of documents and manuscripts that date back centuries. These documents contain valuable genealogical information, including birth, marriage, and death records. This paper aims to describe the preliminary results of the Archivio Storico della Comunita Ebraica di Pisa (ASCEPI) project, with a focus on the extraction of data from the Nati, Morti e Ballottati (NMB) Registry document in the archive. The NMB Registry contains about 1900 records of births, deaths, and balloted individuals within the Jewish community in Pisa. The study uses a semiautomatic pipeline of digitization, transcription, and Natural Language Processing (NLP) techniques to extract personal data such as names, surnames, birth and death dates, and parental names from each record. The extracted data are then used to build a knowledge base and a genealogical tree for a representative family, Supino. This study demonstrates the potential of using NLP and rule-based techniques to extract valuable information from historical documents and to construct genealogical trees.
    Keywords entity extraction ; digital manuscripts ; digital humanities ; genealogical tree ; Information technology ; T58.5-58.64
    Language English
    Publishing date 2023-05-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Anosmin-1-Like Effect of UMODL1/Olfactorin on the Chemomigration of Mouse GnRH Neurons and Zebrafish Olfactory Axons Development

    Elia Di Schiavi / Giulio Vistoli / Roberta Manuela Moretti / Ilaria Corrado / Giulia Zuccarini / Silvia Gervasoni / Lavinia Casati / Daniele Bottai / Giorgio Roberto Merlo / Roberto Maggi

    Frontiers in Cell and Developmental Biology, Vol

    2022  Volume 10

    Abstract: The impairment of development/migration of hypothalamic gonadotropin-releasing hormone (GnRH) neurons is the main cause of Kallmann's syndrome (KS), an inherited disorder characterized by hypogonadism, anosmia, and other developmental defects. Olfactorin ...

    Abstract The impairment of development/migration of hypothalamic gonadotropin-releasing hormone (GnRH) neurons is the main cause of Kallmann's syndrome (KS), an inherited disorder characterized by hypogonadism, anosmia, and other developmental defects. Olfactorin is an extracellular matrix protein encoded by the UMODL1 (uromodulin-like 1) gene expressed in the mouse olfactory region along the migratory route of GnRH neurons. It shares a combination of WAP and FNIII repeats, expressed in complementary domains, with anosmin-1, the product of the ANOS1 gene, identified as the causative of KS. In the present study, we have investigated the effects of olfactorin in vitro and in vivo models. The results show that olfactorin exerts an anosmin-1-like strong chemoattractant effect on mouse-immortalized GnRH neurons (GN11 cells) through the activation of the FGFR and MAPK pathways. In silico analysis of olfactorin and anosmin-1 reveals a satisfactory similarity at the N-terminal region for the overall arrangement of corresponding WAP and FNIII domains and marked similarities between WAP domains’ binding modes of interaction with the resolved FGFR1–FGF2 complex. Finally, in vivo experiments show that the down-modulation of the zebrafish z-umodl1 gene (orthologous of UMODL1) in both GnRH3:GFP and omp2k:gap-CFPrw034 transgenic zebrafish strains leads to a clear disorganization and altered fasciculation of the neurites of GnRH3:GFP neurons crossing at the anterior commissure and a significant increase in olfactory CFP + fibers with altered trajectory. Thus, our study shows olfactorin as an additional factor involved in the development of olfactory and GnRH systems and proposes UMODL1 as a gene worthy of diagnostic investigation in KS.
    Keywords GnRH (gonadotropin-releasing hormone) ; anosmin-1 ; olfactorin ; UMODL1 ; hypothalamus and neuroendocrinology ; olfactory axons ; Biology (General) ; QH301-705.5
    Subject code 572
    Language English
    Publishing date 2022-02-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article: Semi-preparative HPLC purification of δ-tocotrienol (δ-T3) from Elaeis guineensis Jacq. and Bixa orellana L. and evaluation of its in vitro anticancer activity in human A375 melanoma cells

    Beretta, Giangiacomo / Fabrizio Gelmini / Fabrizio Fontana / Roberta Manuela Moretti / Marina Montagnani Marelli / Patrizia Limonta

    Natural product research. 2018 May 19, v. 32, no. 10

    2018  

    Abstract: In this work, we report a rapid and convenient HPLC-UV-DAD method for the isolation of δ-T3 on semi-preparative scale from two different vitamin E rich processed, commercially available products obtained from the fruits of Elaeis guineensis Jacq. (oil ... ...

    Abstract In this work, we report a rapid and convenient HPLC-UV-DAD method for the isolation of δ-T3 on semi-preparative scale from two different vitamin E rich processed, commercially available products obtained from the fruits of Elaeis guineensis Jacq. (oil palm) and from the seeds of Bixa orellana L. (achiote tree). Chromatography was run using reverse phase (RP) C-18 columns and HPLC-grade acetonitrile as mobile phase. The purity of the isolated δ-T3, assessed by GC–MS and ¹H NMR was above 98%. The δ-T3 cytotoxic activity found in vitro against the proliferation of human A375 melanoma cells compared to that of the other δ-T3 free tocols mixture suggest its primary role in the experimental anticancer activity observed for palm oil derived products. Taken altogether, the results of this study highlight the importance of the application of suitable purification systems for the preparations of tocotrienols prior to their experimental or clinical testing.
    Keywords Bixa orellana ; Elaeis guineensis ; acetonitrile ; antineoplastic activity ; cytotoxicity ; delta-tocotrienol ; fruits ; gas chromatography-mass spectrometry ; high performance liquid chromatography ; melanoma ; nuclear magnetic resonance spectroscopy ; seeds ; trees
    Language English
    Dates of publication 2018-0519
    Size p. 1130-1135.
    Publishing place Taylor & Francis
    Document type Article
    ZDB-ID 2185747-7
    ISSN 1478-6427 ; 1478-6419
    ISSN (online) 1478-6427
    ISSN 1478-6419
    DOI 10.1080/14786419.2017.1320793
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Targeting melanoma stem cells with the Vitamin E derivative δ-tocotrienol

    Monica Marzagalli / Roberta Manuela Moretti / Elio Messi / Marina Montagnani Marelli / Fabrizio Fontana / Alessia Anastasia / Maria Rosa Bani / Giangiacomo Beretta / Patrizia Limonta

    Scientific Reports, Vol 8, Iss 1, Pp 1-

    2018  Volume 13

    Abstract: Abstract The prognosis of metastatic melanoma is very poor, due to the development of drug resistance. Cancer stem cells (CSCs) may play a crucial role in this mechanism, contributing to disease relapse. We first characterized CSCs in melanoma cell lines. ...

    Abstract Abstract The prognosis of metastatic melanoma is very poor, due to the development of drug resistance. Cancer stem cells (CSCs) may play a crucial role in this mechanism, contributing to disease relapse. We first characterized CSCs in melanoma cell lines. We observed that A375 (but not BLM) cells are able to form melanospheres and show CSCs traits: expression of the pluripotency markers SOX2 and KLF4, higher invasiveness and tumor formation capability in vivo with respect to parental adherent cells. We also showed that a subpopulation of autofluorescent cells expressing the ABCG2 stem cell marker is present in the A375 spheroid culture. Based on these data, we investigated whether δ-TT might target melanoma CSCs. We demonstrated that melanoma cells escaping the antitumor activity of δ-TT are completely devoid of the ability to form melanospheres. In contrast, cells that escaped vemurafenib treatment show a higher ability to form melanospheres than control cells. δ-TT also induced disaggregation of A375 melanospheres and reduced the spheroidogenic ability of sphere-derived cells, reducing the expression of the ABCG2 marker. These data demonstrate that δ-TT exerts its antitumor activity by targeting the CSC subpopulation of A375 melanoma cells and might represent a novel chemopreventive/therapeutic strategy against melanoma.
    Keywords Medicine ; R ; Science ; Q
    Subject code 570
    Language English
    Publishing date 2018-01-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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