Article ; Online: The Influence of GFRαl Inhibition on Proliferation and Apoptosis of Spermatogenic Cells
Cytol. Genet.. 2022 Dec., v. 56, no. 6 p.567-573
2022
Abstract: The role of the GFRαl receptor in the regulation of cell differentiation and spermatogenesis is poorly investigated. The influence of GFRα1 on the apoptosis and proliferation of spermatogenic cells are not fully elucidated. In this study, GC-1 spg cells ... ...
Abstract | The role of the GFRαl receptor in the regulation of cell differentiation and spermatogenesis is poorly investigated. The influence of GFRα1 on the apoptosis and proliferation of spermatogenic cells are not fully elucidated. In this study, GC-1 spg cells were cultured and then assigned to a control cell (control group) transfected with empty adenovirus AdH1-siRNA and an inhibited cells (inhibited group) with GFRα1 interfering adenovirus (AdH1-siRNA/GFRα1). The GFRal inhibition was determined by employing western blot and qRT-PCR analysis. Furthermore, we used CCK8 for determining the proliferation of the GFRα1 interfering adenovirus (AdH1-siRNA/GFRα1). Transfected cells, employing flow cytometry and Caspase 3, Bax and Bcl-2 expression analysis to determine their apoptosis. This study demonstrated that after transfection, the inhibited cells presented lower proliferation and invasion and higher apoptosis. According to these findings, the inhibition of GFRαl might reduce spermatogenesis and remarkably affect spermatogenic cells proliferation. |
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Keywords | Western blotting ; apoptosis ; caspase-3 ; cell differentiation ; flow cytometry ; spermatogenesis ; transfection |
Language | English |
Dates of publication | 2022-12 |
Size | p. 567-573. |
Publishing place | Pleiades Publishing |
Document type | Article ; Online |
ZDB-ID | 390717-x |
ISSN | 1934-9440 ; 0095-4527 |
ISSN (online) | 1934-9440 |
ISSN | 0095-4527 |
DOI | 10.3103/S0095452722060081 |
Database | NAL-Catalogue (AGRICOLA) |
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