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Article ; Online: Combined RNAscope and immunohistochemistry staining on duodenal paraffin sections as a new tool to reveal cytolytic potential of intraepithelial lymphocytes.

Marano, Antonella / Troncone, Riccardo / Discepolo, Valentina / Maglio, Mariantonia

2023 Volume 517, Page(s) 113470

Abstract: Immunohistochemistry (IHC) is a consolidated technique for the identification of surface and cytoplasmic antigens in cells or tissue sections using specific antibodies, yet simultaneous detection of two markers on the same cell may be difficult to ... ...

Abstract	Immunohistochemistry (IHC) is a consolidated technique for the identification of surface and cytoplasmic antigens in cells or tissue sections using specific antibodies, yet simultaneous detection of two markers on the same cell may be difficult to achieve. Here we develop a protocol to perform a double staining using RNAscope, a new in-situ hybridization (ISH) technology, to visualize perforin transcripts, and classical IHC to visualize either CD8 or TcRγδ positive intraepithelial lymphocytes (IELs) in small intestinal paraffin sections of celiac disease (CD) patients. This double assay will allow to investigate the cytotoxic properties of two subsets of IELs in different stages of CD, thus contributing to understand the events leading to tissue destruction and healing.
MeSH term(s)	Humans ; Paraffin ; Immunohistochemistry ; Intraepithelial Lymphocytes ; Intestine, Small ; Celiac Disease/diagnosis ; In Situ Hybridization ; Intestinal Mucosa
Chemical Substances	Paraffin (8002-74-2)
Language	English
Publishing date	2023-04-08
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	120142-6
ISSN	1872-7905 ; 0022-1759
ISSN (online)	1872-7905
ISSN	0022-1759
DOI	10.1016/j.jim.2023.113470
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Article ; Online: Combined RNAscope and immunohistochemistry staining on duodenal paraffin sections as a new tool to reveal cytolytic potential of intraepithelial lymphocytes

Marano, Antonella / Troncone, R. / Discepolo, Valentina / Maglio, Mariantonia

Journal of Immunological Methods. 2023 June, v. 517 p.113470-

2023

Abstract	Immunohistochemistry (IHC) is a consolidated technique for the identification of surface and cytoplasmic antigens in cells or tissue sections using specific antibodies, yet simultaneous detection of two markers on the same cell may be difficult to achieve. Here we develop a protocol to perform a double staining using RNAscope, a new in-situ hybridization (ISH) technology, to visualize perforin transcripts, and classical IHC to visualize either CD8 or TcRγδ positive intraepithelial lymphocytes (IELs) in small intestinal paraffin sections of celiac disease (CD) patients. This double assay will allow to investigate the cytotoxic properties of two subsets of IELs in different stages of CD, thus contributing to understand the events leading to tissue destruction and healing.
Keywords	celiac disease ; cytotoxicity ; immunohistochemistry ; in situ hybridization ; intestines ; RNAscope ; Intraepithelial lymphocytes ; Perforin ; AMP ; AP ; CD ; CD8 ; FFPE ; HRP ; HybEZ oven ; IELs ; IHC ; ISH ; NKRs ; TcR
Language	English
Dates of publication	2023-06
Publishing place	Elsevier B.V.
Document type	Article ; Online
Note	Pre-press version ; Use and reproduction
ZDB-ID	120142-6
ISSN	1872-7905 ; 0022-1759
ISSN (online)	1872-7905
ISSN	0022-1759
DOI	10.1016/j.jim.2023.113470
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Article: Ectopic decidual reaction.

MARANO, A

Archivos. Sociedad Argentina de Anatomia Normal y Patologica

2010 Volume 8, Issue 2, Page(s) 213–217

Title translation	Reaccion decidual ectopica.
MeSH term(s)	Female ; Humans ; Pregnancy
Language	Spanish
Publishing date	2010-02-28
Publishing country	Argentina
Document type	Journal Article
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Article: Myxoma of the breast.

MARANO, A

Revista de la Asociacion Medica Argentina

2010 Volume 59, Page(s) 1415

Title translation	Mixoma de mama.
MeSH term(s)	Breast Neoplasms ; Humans ; Myxoma ; Neoplasms
Language	Spanish
Publishing date	2010-08-27
Publishing country	Argentina
Document type	Journal Article
ZDB-ID	422344-5
ISSN	0004-4830
ISSN	0004-4830
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Article ; Online: Biosorption of rare earth elements from luminophores by G. sulphuraria (Cyanidiophytina, Rhodophyta).

Iovinella, M / Palmieri, M / Papa, S / Auciello, C / Ventura, R / Lombardo, F / Race, M / Lubritto, C / di Cicco, M R / Davis, S J / Trifuoggi, M / Marano, A / Ciniglia, C

Environmental research

2023 Volume 239, Issue Pt 1, Page(s) 117281

Abstract: Lanthanides are indispensable constituents of modern technologies and are often challenging to acquire from natural resources. The demand for REEs is so high that there is a clear need to develop efficient and eco-friendly recycling methods. In the ... ...

Abstract	Lanthanides are indispensable constituents of modern technologies and are often challenging to acquire from natural resources. The demand for REEs is so high that there is a clear need to develop efficient and eco-friendly recycling methods. In the present study, freeze-dried biomass of the polyextremophile Galdieria sulphuraria was employed to recover REEs from spent fluorescent lamps (FL) luminophores by pretreating the freeze-dried biomass with an acid solution to favour ion exchange and enhance the binding sites on the cell surface available for the metal ions. Lanthanides were extracted from the luminophores using sulfuric acid solutions according to standardised procedures, and the effect of biosorbent dosage (0.5-5 mg/ml) and biosorption time (5-60 min) were evaluated. The content of individual REEs in the luminophores and the resulting algal biomass were determined using inductively coupled plasma mass spectrometry (ICP-MS). The most abundant REE in the luminophores was yttrium (287.42 mg/g dm, 91.60% of all REEs), followed by europium (20.98 mg/g, 6.69%); cerium, gadolinium, terbium and lanthanum was in trace. The best biosorption performances were achieved after 5 min and at the lowest biosorbent dosage (0.5 mg/mL). The highest total metal amount corresponded to 41.61 mg/g dried mass, and yttrium was the most adsorbed metal (34.59 mg/g dm, 82.88%), followed by cerium (4.01 mg/g); all other metals were less than 2 mg/g. The rapidity of the biosorption process and the low biosorbent dosage required confirmed this microalga as a promising material for creating an eco-sustainable protocol for recycling REEs.
MeSH term(s)	Metals, Rare Earth/analysis ; Cerium ; Yttrium ; Metals/metabolism ; Rhodophyta/metabolism
Chemical Substances	Metals, Rare Earth ; Cerium (30K4522N6T) ; Yttrium (58784XQC3Y) ; Metals
Language	English
Publishing date	2023-10-10
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	205699-9
ISSN	1096-0953 ; 0013-9351
ISSN (online)	1096-0953
ISSN	0013-9351
DOI	10.1016/j.envres.2023.117281
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Article: Robotic Intraoperative Tracheobronchial Repair during Minimally Invasive 3-Stage Esophagectomy.

Marano, Alessandra / Palagi, Silvia / Pellegrino, Luca / Borghi, Felice

Journal of chest surgery

2020 Volume 54, Issue 2, Page(s) 154–157

Abstract: Tracheobronchial injury (TBI) is an uncommon but potentially fatal event. Iatrogenic lesions during bronchoscopy, endotracheal intubation, or thoracic surgery are considered the most common causes of TBI. When TBI is detected during surgery, concomitant ... ...

Abstract	Tracheobronchial injury (TBI) is an uncommon but potentially fatal event. Iatrogenic lesions during bronchoscopy, endotracheal intubation, or thoracic surgery are considered the most common causes of TBI. When TBI is detected during surgery, concomitant surgical treatment is recommended. Herein we present a case of successful robotic primary repair of iatrogenic tracheal and left bronchial branch tears during a robot-assisted hybrid 3-stage esophagectomy after neoadjuvant chemoradiotherapy. A robotic approach can facilitate the repair of this injury while reducing both the potential risk of conversion to open surgery and the associated increased risk of postoperative respiratory complications.
Language	English
Publishing date	2020-10-28
Document type	Case Reports
ISSN	2765-1606
ISSN	2765-1606
DOI	10.5090/jcs.20.055
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Article ; Online: Reconstruction of Through-and-Through Mandibular Defects with the Fibula Osteocutaneous and Lower Lateral Leg Perforator Free Flaps.

Shahzad, Farooq / Kiwanuka, Elizabeth / Marano, Andrew / Boyle, Jay / Matros, Evan

Plastic and reconstructive surgery

2021 Volume 149, Issue 1, Page(s) 157e–158e

MeSH term(s)	Carcinoma, Squamous Cell/pathology ; Carcinoma, Squamous Cell/surgery ; Fibula/transplantation ; Free Tissue Flaps/transplantation ; Humans ; Leg/blood supply ; Leg/surgery ; Male ; Mandible/pathology ; Mandible/surgery ; Mandibular Neoplasms/pathology ; Mandibular Neoplasms/surgery ; Perforator Flap/transplantation ; Surgical Wound/etiology ; Surgical Wound/surgery ; Treatment Outcome
Language	English
Publishing date	2021-11-22
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	208012-6
ISSN	1529-4242 ; 0032-1052 ; 0096-8501
ISSN (online)	1529-4242
ISSN	0032-1052 ; 0096-8501
DOI	10.1097/PRS.0000000000008620
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Article ; Online: Robot-assisted esophagectomy with robot-sewn intrathoracic anastomosis (Ivor Lewis): surgical technique and early results.

Marano, Alessandra / Salomone, Sara / Pellegrino, Luca / Geretto, Paolo / Robella, Manuela / Borghi, Felice

Updates in surgery

2022 Volume 75, Issue 4, Page(s) 941–952

Abstract: Esophagectomy is the selected treatment for nonmetastatic esophageal and esophagogastric junction cancer, although high perioperative morbidity and mortality incur. Robot-assisted minimally invasive esophagectomy (RAMIE) effectively reduces ... ...

Abstract	Esophagectomy is the selected treatment for nonmetastatic esophageal and esophagogastric junction cancer, although high perioperative morbidity and mortality incur. Robot-assisted minimally invasive esophagectomy (RAMIE) effectively reduces cardiopulmonary complications compared to open esophagectomy and offers a technical advantage, especially for lymph node dissection and intrathoracic anastomosis. This article aims at describing our initial experience of Ivor Lewis RAMIE, focusing on the technique's main steps and robotic-sewn esophagogastrostomy. Prospectively collected data from all consecutive patients who underwent Ivor Lewis RAMIE for cancer was reviewed. Reconstruction was performed with a gastric conduit pull-up and a robotic-sewn intrathoracic anastomosis. Intraoperative and postoperative complications were recorded as prescribed by the Esophagectomy Complications Consensus Group (ECCG). Thirty patients underwent Ivor Lewis RAMIE with complete mediastinal lymph node dissection and robot-sewn anastomosis. No intraoperative complications nor conversion occurred. Pulmonary complications totaled 26.7%. Anastomotic leakage (ECCG, type III) and conduit necrosis (ECCG, type III) both occurred in one patient (3.3%). Chylothorax appeared in 2 patients (6.7%) (ECCG, Type IIA). Anastomotic stricture, successfully treated with endoscopic dilatations, occurred in 8 cases (26.7%). Median overall postoperative stay was 11 days (range, 6-51 days). 30 day and 90 day mortality was 0%. R0 resection was performed in 96.7% of patients with a median number of 47 retrieved lymph nodes. RAMIE with robot-sewn intrathoracic anastomosis appears to be feasible, safe and effective, with favorable perioperative results. Nevertheless, further high-quality studies are needed to define the best anastomotic technique for Ivor Lewis RAMIE.
MeSH term(s)	Humans ; Esophagectomy/methods ; Robotics ; Robotic Surgical Procedures/methods ; Esophageal Neoplasms/pathology ; Retrospective Studies ; Anastomosis, Surgical/methods ; Postoperative Complications/epidemiology ; Postoperative Complications/surgery ; Treatment Outcome
Language	English
Publishing date	2022-12-12
Publishing country	Italy
Document type	Journal Article
ZDB-ID	2572692-4
ISSN	2038-3312 ; 2038-131X
ISSN (online)	2038-3312
ISSN	2038-131X
DOI	10.1007/s13304-022-01439-7
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Article ; Online: Mucosal Healing in Celiac Disease: Villous Architecture and Immunohistochemical Features in Children on a Long-Term Gluten Free Diet.

Mandile, Roberta / Maglio, Mariantonia / Mosca, Caterina / Marano, Antonella / Discepolo, Valentina / Troncone, Riccardo / Auricchio, Renata

Nutrients

2022 Volume 14, Issue 18

Abstract: Considerable heterogeneity exists across studies assessing intestinal mucosal recovery in celiac (CD) patients on a gluten-free diet (GFD). We aimed at investigating histological and immunohistochemical features in CD patients on a long-term GFD and to ... ...

Abstract	Considerable heterogeneity exists across studies assessing intestinal mucosal recovery in celiac (CD) patients on a gluten-free diet (GFD). We aimed at investigating histological and immunohistochemical features in CD patients on a long-term GFD and to correlate them to the GFD duration. Morphometrical and immunohistochemical analysis were retrospectively performed on duodenal biopsies in three groups of children: 33 on a long-term (>2 years) GFD (GFD-group), four of which remained seropositive despite dietary adherence, 31 with villous atrophy (ACD-group) and 76 heathy, non-celiac (CTR-group). Moreover, in the GFD-group, we correlated immunohistochemical alterations to the GFD duration. The villous to crypt (V/C) ratio significantly improved after the GFD and completely normalized in all patients, becoming even higher than in the CTR-group (median value 3.2 vs. 3, p = 0.007). In parallel, the number of CD3+ and TCRγδ+ cells in the epithelium were significantly reduced in the GFD compared to ACD patients, even if they remained higher than in the CTR-group (p < 0.05). In contrast, CD25+ cells in the lamina propria significantly decreased after the GFD (p < 0.05) and become comparable to the CTR-group (p = 0.9). In the GFD-group there was no difference in the immunohistochemical parameters between seropositive and seronegative patients and alterations did not correlate to GFD length. In conclusion, a GFD is able to both restore a normal V/C ratio and reduce inflammation, but the epithelium maintains some stigmata of the disorder, such as an increased number of CD3+ and TCRγδ+ cells. These alterations persist regardless of the duration of the GFD.
MeSH term(s)	Biopsy ; Celiac Disease ; Child ; Diet, Gluten-Free ; Humans ; Intestinal Mucosa/pathology ; Retrospective Studies
Language	English
Publishing date	2022-09-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2518386-2
ISSN	2072-6643 ; 2072-6643
ISSN (online)	2072-6643
ISSN	2072-6643
DOI	10.3390/nu14183696
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Article: Abdominal wall endometriosis misdiagnosed as a desmoid tumor: A case report.

Girardi, Martina / Marano, Alessandra / Fortunato, Mirella / Gelarda, Enrico / Giuffrida, Maria Carmela

International journal of surgery case reports

2022 Volume 93, Page(s) 106979

Abstract: Introduction: Abdominal wall masses have different aetiologies. Diagnosis includes desmoid tumors (DTs) and other benign and malignant lesions, among which abdominal wall endometriosis (AWE). Diagnosis is challenging if symptoms are aspecific, and the ... ...

Abstract	Introduction: Abdominal wall masses have different aetiologies. Diagnosis includes desmoid tumors (DTs) and other benign and malignant lesions, among which abdominal wall endometriosis (AWE). Diagnosis is challenging if symptoms are aspecific, and the contribution of imaging may be weak. We present a case of AWE that according to clinical history and imaging was misdiagnosed as DT. Presentation of case: A healthy 35-year-old female presented, 4 years after a cesarean delivery, a rapidly growing painless subumbilical mass within the right rectus abdominis muscle. Ultrasound and magnetic resonance imaging suspected a DT. The patient underwent complete resection of the mass and pathological examination revealed foci of endometriosis in the muscle. Patient's post-operative course was uneventful and at 18-month follow-up, no recurrence has been detected. Discussion: The current case highlights differences in clinical presentation and imaging in case of AWE and DTs, underlining possible pitfalls in diagnosis. In young women with previous gynaecological abdominal surgery, AWE is the most likely disease when a mass in the region of the scar appears. Differential diagnosis is complex and rare entities like DTs should nevertheless be taken into consideration. A complete surgical resection with negative margins is considered the primary treatment for AWE and for selected DTs. Final pathology of the tumor can state the precise diagnosis. Conclusion: Since AWE and DTs share similar clinical signs and aspecific imaging exams, both diseases should be considered in case of abdominal wall mass in female patients of childbearing age and history of uterine-related surgery.
Language	English
Publishing date	2022-03-29
Publishing country	Netherlands
Document type	Journal Article
ISSN	2210-2612
ISSN	2210-2612
DOI	10.1016/j.ijscr.2022.106979
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