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Article ; Online: "The Future of Vulvar Disease: New and Ongoing Challenges": Presidential Lecture - XXIV World Congress 2017, Mendoza, Argentina.

Marchitelli, Claudia

2018 Volume 22, Issue 2, Page(s) 166–168

Abstract: The 21st century brought along a number of discoveries and medical advances. With the advances, we can mention the following: target therapies for cancer, genomics and molecular biology, pharmacogenomics, minimally invasive and robotic surgery, ... ...

Abstract	The 21st century brought along a number of discoveries and medical advances. With the advances, we can mention the following: target therapies for cancer, genomics and molecular biology, pharmacogenomics, minimally invasive and robotic surgery, immunosuppressive therapies in transplant patients, human papillomavirus in the era of vaccination, and aesthetic vulvovaginal procedures. All of them have greatly influenced medicine. As a scientific society, we have several challenges. In the past, you had to go to a library to get information. Now, we have readily available, accessible information. Thus, here comes the debate; we are facing a change in our educational paradigm, which needs to be approached from an integral and holistic perspective. New historical and cultural circumstances call for a deep reflection on the current social validity of scientific societies. They should be open to new ideas and allow dissent, work in teams, and speak a common language. They must be organized and, in constant communication with its members, support and organize research developing well-designed clinical trials and observational studies. We should work for our patients, recognizing the importance of the whole woman. We all have a great scientific and humanitarian challenge; to succeed, we must not lose sight of who we are working for. We work for them, our patients, and not for us. Our mission is to take care of our them!
MeSH term(s)	Argentina ; Female ; Humans ; Pharmacogenetics ; Robotic Surgical Procedures ; Societies, Scientific ; Vulvar Diseases/therapy
Language	English
Publishing date	2018-02-23
Publishing country	United States
Document type	Journal Article ; Lectures
ZDB-ID	2041332-4
ISSN	1526-0976 ; 1089-2591
ISSN (online)	1526-0976
ISSN	1089-2591
DOI	10.1097/LGT.0000000000000379
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Article ; Online: Determination of

Crisà, Alessandra / Marchitelli, Cinzia / Failla, Sebastiana / Contò, Michela

The Journal of dairy research

2022 , Page(s) 1–3

Abstract: This research communication reports concentrations of two sialic acids (SA), ...

Abstract	This research communication reports concentrations of two sialic acids (SA),
Language	English
Publishing date	2022-09-26
Publishing country	England
Document type	Journal Article
ZDB-ID	242089-2
ISSN	1469-7629 ; 0022-0299
ISSN (online)	1469-7629
ISSN	0022-0299
DOI	10.1017/S0022029922000620
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Article ; Online: Genetic parameters and genome-wide associations of twinning rate in a local breed, the Maremmana cattle.

Moioli, B / Steri, R / Marchitelli, C / Catillo, G / Buttazzoni, L

Animal : an international journal of animal bioscience

2017 Volume 11, Issue 10, Page(s) 1660–1666

Abstract: This study seeks to verify the feasibility of increasing twinning in a herd of the Italian autochtonous Maremmana breed. The data set included 1260 individuals born from 1963 to 2014, 527 males and 733 females, 402 of them calving at least once from 1983 ...

Abstract	This study seeks to verify the feasibility of increasing twinning in a herd of the Italian autochtonous Maremmana breed. The data set included 1260 individuals born from 1963 to 2014, 527 males and 733 females, 402 of them calving at least once from 1983 through 2015. Breeding values for twinning were estimated by a single-trait linear animal model. However, since twinning is a dichotomous trait and the frequency of twins is far smaller than the frequency of single births, breeding values were also estimated by a single-trait animal threshold model. Heritability of twinning was 0.014±0.018 and 0.062±0.093 for the linear and the threshold models, respectively. Repeatability was 0.071±0.004 and 0.286± 0.012, respectively, for the two models. Genotyping with the Illumina BovineSNP54 BeadChip was performed for cows living on farm in 2012 (119 cows) and a genome-wide association analysis was performed on the corrected phenotype of all calving during the lifespan of each cow, using the GenABEL package in R and a three step GRAMMAR-GC approach. Genomic heritability, calculated from the genomic kinship matrix estimated through genomic marker data, was 0.29±0.021. The most significant detected single nucleotide polymorphisms (Hapmap22923-BTA-129564) was located in proximity of two genes, ARHGAP8 and TMEM200C, which might be potential functional candidates for twinning rate in cattle.
Language	English
Publishing date	2017-10
Publishing country	England
Document type	Journal Article
ZDB-ID	2257920-5
ISSN	1751-732X ; 1751-7311
ISSN (online)	1751-732X
ISSN	1751-7311
DOI	10.1017/S1751731117000283
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Article ; Online: Identification of the complete coding cDNAs and expression analysis of B4GALT1, LALBA, ST3GAL5, ST6GAL1 in the colostrum and milk of the Garganica and Maltese goat breeds to reveal possible implications for oligosaccharide biosynthesis.

Crisà, Alessandra / Claps, Salvatore / Moioli, Bianca / Marchitelli, Cinzia

BMC veterinary research

2019 Volume 15, Issue 1, Page(s) 457

Abstract: Background: Milk sialylated oligosaccharides (SOS) play crucial roles in many biological processes. The most abundant free SOS in goat's milk are 3'sialyllactose (3'-SL), 6'sialyllactose (6'-SL) and disialyllactose (DSL). The production of these ... ...

Abstract	Background: Milk sialylated oligosaccharides (SOS) play crucial roles in many biological processes. The most abundant free SOS in goat's milk are 3'sialyllactose (3'-SL), 6'sialyllactose (6'-SL) and disialyllactose (DSL). The production of these molecules is determined genetically by the expression of glycosyltransferases and by the availability of nucleotide sugar substrates, but the precise mechanisms regulating the differential patterns of milk oligosaccharides are not known. We aimed to identify the complete cDNAs of candidate genes implicated in SOS biosynthesis (B4GALT1, LALBA, ST3GAL5, ST6GAL1) and to analyse their expression during lactation in the Garganica and Maltese goat breeds. Moreover, we analysed the colostrum and milk contents of 3'-SL, 6'-SL and disialyllactose (DSL) and the possible correlations between expressed genes and SOS. Results: We identified the complete coding cDNAs of B4GALT1 (HQ700335.1), ST3GAL5 (KF055858.2), and ST6GAL1 (HQ709167.1), the single nucleotide polymorphism (SNPs) of these genes and 2 splicing variants of the ST6GAL1 cDNA. RT-qPCR analysis showed that LALBA and ST6GAL1 were the genes with the highest and lowest expression in both breeds, respectively. The interaction effects of the breeds and sampling times were associated with higher levels of B4GALT1 and ST3GAL5 gene expression in Garganica than in Maltese goats at kidding. B4GALT1, LALBA, and ST3GAL5 gene expression changed from kidding to 60 and 120 days in Maltese goats, while in Garganica goats, a difference was observed only for the LALBA gene. Breed and lactation effects were also found for SOS contents. Positive correlations of B4GALT1, LALBA, ST3GAL5, and ST6GAL1 with 3'-SL/6'SL and DSL were found. Conclusions: The genetic effect on the oligosaccharide content of milk was previously highlighted in bovines, and this study is the first to investigate this effect in two goat breeds (Garganica and Maltese) during lactation. The genetic variability of candidate genes involved in SOS biosynthesis highlights their potential role in affecting gene expression and ultimately biological function. The investigation of gene regulatory regions as well as the examination of other sialyltransferase genes will be needed to identify the genetic pattern leading to a higher SOS content in the autochtonous Garganica breed and to protect it using a focused breeding strategy.
MeSH term(s)	Animals ; Breeding ; Colostrum/chemistry ; DNA, Complementary/analysis ; Female ; Gene Expression Profiling ; Goats/classification ; Goats/genetics ; Goats/metabolism ; Lactation ; Milk/chemistry ; Oligosaccharides/biosynthesis ; Oligosaccharides/genetics ; Polymorphism, Single Nucleotide ; Pregnancy
Chemical Substances	DNA, Complementary ; Oligosaccharides
Language	English
Publishing date	2019-12-18
Publishing country	England
Document type	Journal Article
ISSN	1746-6148
ISSN (online)	1746-6148
DOI	10.1186/s12917-019-2206-0
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Article ; Online: Gelsolin expression in sheep milk somatic cells during lactation.

Napolitano, F / Grandoni, F / Signorelli, F / Annicchiarico, G / Catillo, G / Moioli, B / Crisà, A / Marchitelli, C

Animal : an international journal of animal bioscience

2019 Volume 13, Issue 10, Page(s) 2297–2304

Abstract: The identification of genes involved in phenotypes related to milk quality is important for both economic and health aspects in livestock production. The aim of this study was to assess the level of gelsolin gene expression in two breeds of dairy sheep - ...

Abstract	The identification of genes involved in phenotypes related to milk quality is important for both economic and health aspects in livestock production. The aim of this study was to assess the level of gelsolin gene expression in two breeds of dairy sheep - Sarda and Gentile - with pronounced differences in quantitative and qualitative milk traits. Gelsolin, a type of actin-modulating proteins is involved in the processes of actin remodeling during cell growth and apoptosis; therefore a role of this protein in mammary changes during lactation was here hypothesized. Individual milk samples were collected three times during lactation from 26 ewes of the two breeds. The differential gene expression of gelsolin in the two breeds and the three lactation times was estimated by quantitative PCR on RNA extracted from milk somatic cells. Correlations of gelsolin gene expression with milk yield and quality and days of lactation were also estimated. The results showed that gelsolin gene expression was significantly higher in the Sarda compared to the Gentile at each lactation stage, in agreement with the longer lactation duration and the higher daily milk yield of the first breed. Significant correlations of gelsolin gene expression were found with milk fat content in Sarda breed (-0.46, P<0.05). Gelsolin expression analysis confirmed the link between gelsolin gene function and milk fat content of sheep.
MeSH term(s)	Animals ; Female ; Gelsolin/genetics ; Gene Expression Regulation ; Lactation ; Milk/metabolism ; Milk/standards ; Phenotype ; Sheep/genetics ; Sheep/physiology ; Time Factors
Chemical Substances	Gelsolin
Language	English
Publishing date	2019-03-06
Publishing country	England
Document type	Journal Article
ZDB-ID	2257920-5
ISSN	1751-732X ; 1751-7311
ISSN (online)	1751-732X
ISSN	1751-7311
DOI	10.1017/S1751731119000399
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Article ; Online: Prediction of key milk biomarkers in dairy cows through milk mid-infrared spectra and international collaborations.

Grelet, C / Larsen, T / Crowe, M A / Wathes, D C / Ferris, C P / Ingvartsen, K L / Marchitelli, C / Becker, F / Vanlierde, A / Leblois, J / Schuler, U / Auer, F J / Köck, A / Dale, L / Sölkner, J / Christophe, O / Hummel, J / Mensching, A / Fernández Pierna, J A /

Soyeurt, H / Calmels, M / Reding, R / Gelé, M / Chen, Y / Gengler, N / Dehareng, F

Journal of dairy science

2023 Volume 107, Issue 3, Page(s) 1669–1684

Abstract: At the individual cow level, suboptimum fertility, mastitis, negative energy balance, and ketosis are major issues in dairy farming. These problems are widespread on dairy farms and have an important economic impact. The objectives of this study were (1) ...

Abstract	At the individual cow level, suboptimum fertility, mastitis, negative energy balance, and ketosis are major issues in dairy farming. These problems are widespread on dairy farms and have an important economic impact. The objectives of this study were (1) to assess the potential of milk mid-infrared (MIR) spectra to predict key biomarkers of energy deficit (citrate, isocitrate, glucose-6 phosphate [glucose-6P], free glucose), ketosis (β-hydroxybutyrate [BHB] and acetone), mastitis (N-acetyl-β-d-glucosaminidase activity [NAGase] and lactate dehydrogenase), and fertility (progesterone); (2) to test alternative methodologies to partial least squares (PLS) regression to better account for the specific asymmetric distribution of the biomarkers; and (3) to create robust models by merging large datasets from 5 international or national projects. Benefiting from this international collaboration, the dataset comprised a total of 9,143 milk samples from 3,758 cows located in 589 herds across 10 countries and represented 7 breeds. The samples were analyzed by reference chemistry for biomarker contents, whereas the MIR analyses were performed on 30 instruments from different models and brands, with spectra harmonized into a common format. Four quantitative methodologies were evaluated to address the strongly skewed distribution of some biomarkers. Partial least squares regression was used as the reference basis, and compared with a random modification of distribution associated with PLS (random-downsampling-PLS), an optimized modification of distribution associated with PLS (KennardStone-downsampling-PLS), and support vector machine (SVM). When the ability of MIR to predict biomarkers was too low for quantification, different qualitative methodologies were tested to discriminate low versus high values of biomarkers. For each biomarker, 20% of the herds were randomly removed within all countries to be used as the validation dataset. The remaining 80% of herds were used as the calibration dataset. In calibration, the 3 alternative methodologies outperform the PLS performances for the majority of biomarkers. However, in the external herd validation, PLS provided the best results for isocitrate, glucose-6P, free glucose, and lactate dehydrogenase (coefficient of determination in external herd validation [R
MeSH term(s)	Female ; Cattle ; Animals ; Milk ; Isocitrates ; Acetone ; Acetylglucosaminidase ; Progesterone ; Citrates ; Citric Acid ; 3-Hydroxybutyric Acid ; Biomarkers ; Glucose ; Ketosis/diagnosis ; Ketosis/veterinary ; L-Lactate Dehydrogenase ; Mastitis/veterinary ; Cattle Diseases
Chemical Substances	isocitric acid (9RW6G5D4MQ) ; Isocitrates ; Acetone (1364PS73AF) ; Acetylglucosaminidase (EC 3.2.1.52) ; Progesterone (4G7DS2Q64Y) ; Citrates ; Citric Acid (2968PHW8QP) ; 3-Hydroxybutyric Acid (TZP1275679) ; Biomarkers ; Glucose (IY9XDZ35W2) ; L-Lactate Dehydrogenase (EC 1.1.1.27)
Language	English
Publishing date	2023-10-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	242499-x
ISSN	1525-3198 ; 0022-0302
ISSN (online)	1525-3198
ISSN	0022-0302
DOI	10.3168/jds.2023-23843
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Article ; Online: The "Genitourinary Syndrome of Menopause": A Leap Forward?

Vieira-Baptista, Pedro / Marchitelli, Claudia / Haefner, Hope K

Journal of lower genital tract disease

2015 Volume 19, Issue 4, Page(s) 362–363

MeSH term(s)	Atrophy/pathology ; Female ; Humans ; Menopause/physiology ; Urogenital System/pathology ; Urogenital System/physiology
Language	English
Publishing date	2015-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	2041332-4
ISSN	1526-0976 ; 1089-2591
ISSN (online)	1526-0976
ISSN	1089-2591
DOI	10.1097/LGT.0000000000000127
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Article ; Online: Labioplasty specimens: when should pathological findings be considered as abnormal?

Scurry, James / Marchitelli, Claudia / Moyal-Barracco, Micheline

Journal of lower genital tract disease

2015 Volume 19, Issue 2, Page(s) e49–50

MeSH term(s)	Animals ; Female ; Humans ; Hypertrophy/etiology ; Hypertrophy/pathology ; Lymphedema/complications ; Lymphedema/pathology ; Vulvar Diseases/etiology ; Vulvar Diseases/pathology
Language	English
Publishing date	2015-04
Publishing country	United States
Document type	Comment ; Letter
ZDB-ID	2041332-4
ISSN	1526-0976 ; 1089-2591
ISSN (online)	1526-0976
ISSN	1089-2591
DOI	10.1097/LGT.0000000000000094
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Article: Gelsolin expression in sheep milk somatic cells during lactation

Napolitano, F / Annicchiarico, G / Catillo, G / Crisà, A / Grandoni, F / Marchitelli, C / Moioli, B / Signorelli, F

Animal. 2019 Oct., v. 13, no. 10

2019

Abstract	The identification of genes involved in phenotypes related to milk quality is important for both economic and health aspects in livestock production. The aim of this study was to assess the level of gelsolin gene expression in two breeds of dairy sheep – Sarda and Gentile – with pronounced differences in quantitative and qualitative milk traits. Gelsolin, a type of actin-modulating proteins is involved in the processes of actin remodeling during cell growth and apoptosis; therefore a role of this protein in mammary changes during lactation was here hypothesized. Individual milk samples were collected three times during lactation from 26 ewes of the two breeds. The differential gene expression of gelsolin in the two breeds and the three lactation times was estimated by quantitative PCR on RNA extracted from milk somatic cells. Correlations of gelsolin gene expression with milk yield and quality and days of lactation were also estimated. The results showed that gelsolin gene expression was significantly higher in the Sarda compared to the Gentile at each lactation stage, in agreement with the longer lactation duration and the higher daily milk yield of the first breed. Significant correlations of gelsolin gene expression were found with milk fat content in Sarda breed (−0.46, P<0.05). Gelsolin expression analysis confirmed the link between gelsolin gene function and milk fat content of sheep.
Keywords	actin ; apoptosis ; breeds ; cell growth ; dairy sheep ; ewe milk ; ewes ; gene expression ; gene expression regulation ; genes ; lactation duration ; lactation stage ; lipid content ; livestock production ; milk ; milk fat ; milk quality ; milk yield ; phenotype ; quantitative polymerase chain reaction ; RNA ; somatic cells
Language	English
Dates of publication	2019-10
Size	p. 2297-2304.
Publishing place	Cambridge University Press
Document type	Article
ZDB-ID	2257920-5
ISSN	1751-732X ; 1751-7311
ISSN (online)	1751-732X
ISSN	1751-7311
DOI	10.1017/S1751731119000399
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: To the Editor.

Vieira-Baptista, Pedro / Damaser, Margot / Digesu, Alex / Marchitelli, Claudia / Preti, Mario / Stockdale, Colleen

Menopause (New York, N.Y.)

2018 Volume 25, Issue 10, Page(s) 1166–1167

MeSH term(s)	Female ; Humans ; Lasers, Gas ; Menopause ; Syndrome ; Vulva
Language	English
Publishing date	2018-07-04
Publishing country	United States
Document type	Journal Article ; Comment
ZDB-ID	1205262-0
ISSN	1530-0374 ; 1072-3714
ISSN (online)	1530-0374
ISSN	1072-3714
DOI	10.1097/GME.0000000000001165
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