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  1. Article ; Online: SARS-CoV-2 diagnostic diary

    Marcio da Costa Cipitelli / Elizabeth Valentin / Nadia Vaez Gonçalves da Cruz / Tatiana LS Nogueira / Elaine Cristina Amaro de Melo / Rebeca Araujo da Silva / Marcelo M Serra / André L Meriano / Alberto ML Colares / Marcos Dornelas-Ribeiro / Caleb GM Santos

    Memórias do Instituto Oswaldo Cruz., Vol

    from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro

    2020  Volume 115

    Abstract: Corona virus disease (COVID-19) presents a serious threat to global health. A historical timeline of early molecular diagnostics from government alert (January 22) (D) was presented. After in silico analysis, Brazilian Army Institute of Biology (IBEx-RJ) ...

    Abstract Corona virus disease (COVID-19) presents a serious threat to global health. A historical timeline of early molecular diagnostics from government alert (January 22) (D) was presented. After in silico analysis, Brazilian Army Institute of Biology (IBEx-RJ) tested samples in house using real-time reverse transcriptase polymerase chain reaction (RT-PCR) (fast mode) based on Centers for Disease Control and Prevention (CDC) recommendations. First cases from Brazil, Rio de Janeiro, IBEx, and diagnosis team were reported in D36, D44, D66, and D74 respectively. Therefore, after 1300 tests, we recommend N1/N2 primer sets (CDC) for preliminary and Charité protocol confirmation in case of positive results. Moreover, every professional should be tested before starting work, in addition to weekly tests for everyone involved.
    Keywords coronavirus ; SARS-CoV-2 ; RT-PCR ; global pandemic ; COVID-19 ; Arctic medicine. Tropical medicine ; RC955-962 ; Microbiology ; QR1-502
    Language English
    Publishing date 2020-07-01T00:00:00Z
    Publisher Instituto Oswaldo Cruz, Ministério da Saúde
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: Trichosporon asahii secretes a 30-kDa aspartic peptidase

    Valle, Roberta S / André L.S. Santos / Cátia L. Sodré / Lívia S. Ramos / Marcos Dornelas-Ribeiro / Mariangela Ziccardi / Marta H. Branquinha / Vanessa J. Reis

    Microbiological research. 2017 Dec., v. 205

    2017  

    Abstract: Trichosporon asahii is a fungal opportunistic pathogen that causes superficial and deep-seated infections presenting high mortality. Very little is known about the virulence attributes produced by this fungus. Herein, aspartic peptidase production was ... ...

    Abstract Trichosporon asahii is a fungal opportunistic pathogen that causes superficial and deep-seated infections presenting high mortality. Very little is known about the virulence attributes produced by this fungus. Herein, aspartic peptidase production was identified in Brazilian clinical isolates of T. asahii by different methodologies. Initially, T. asahii strain 250 (from skin lesion) was inoculated in both liquid and solid culture media containing bovine serum albumin (BSA) as the sole nitrogenous source. A translucent halo around the fungal colony was observed from the 5th day of culture. The cell-free culture supernatant revealed that soluble BSA was hydrolyzed along the growth, generating low molecular mass polypeptides as observed by electrophoresis. Subsequently, the secretions from four clinical strains of T. asahii were analyzed by BSA-SDS-PAGE and a single proteolytic band of 30-kDa was detected under acidic pH at 37°C. The secreted aspartic peptidase of T. asahii efficiently cleaved the cathepsin D peptide substrate, but not the substrates with specificity to HIV-1 peptidase and rennin. The capability to cleave either cathepsin D substrate in a fluorogenic assay or BSA immobilized within a gel matrix varied according to the T. asahii isolate. T. asahii extracellular peptidase activity was strongly inhibited by pepstatin A and HIV peptidase inhibitors, classifying it as an aspartic-type peptidase. Human serum albumin, mucin, non-immune immunoglobulin G and gelatin induced, in different levels, the secretion of this aspartic peptidase. With these results, T. asahii must be included in the list of many human fungal opportunistic pathogens able to secrete an aspartic-type peptidase.
    Keywords bovine serum albumin ; cathepsin D ; cell free system ; chymosin ; culture media ; electrophoresis ; fungi ; gelatin ; Human immunodeficiency virus 1 ; human serum albumin ; humans ; immunoglobulin G ; molecular weight ; mortality ; mucins ; pH ; polypeptides ; proteolysis ; secondary infection ; secretion ; skin lesions ; Trichosporon asahii ; virulence
    Language English
    Dates of publication 2017-12
    Size p. 66-72.
    Publishing place Elsevier GmbH
    Document type Article
    ZDB-ID 1189614-0
    ISSN 1618-0623 ; 0944-5013
    ISSN (online) 1618-0623
    ISSN 0944-5013
    DOI 10.1016/j.micres.2017.08.015
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  3. Article ; Online: Nosocomial candidiasis in Rio de Janeiro State

    Paulo Murillo Neufeld / Marcia de Souza Carvalho Melhem / Maria Walderez Szeszs / Marcos Dornelas Ribeiro / Efigênia de Lourdes Teixeira Amorim / Manuela da Silva / Marcia dos Santos Lazéra

    Brazilian Journal of Microbiology, Vol 46, Iss 2, Pp 477-

    Distribution and fluconazole susceptibility profile

    2015  Volume 484

    Abstract: One hundred and forty-one Candida species isolated from clinical specimens of hospitalized patients in Rio de Janeiro, Brazil, during 2002 to 2007, were analized in order to evaluate the distribution and susceptibility of these species to fluconazole. ... ...

    Abstract One hundred and forty-one Candida species isolated from clinical specimens of hospitalized patients in Rio de Janeiro, Brazil, during 2002 to 2007, were analized in order to evaluate the distribution and susceptibility of these species to fluconazole. Candida albicans was the most frequent species (45.4%), followed by C. parapsilosis sensu lato (28.4%), C. tropicalis (14.2%), C. guilliermondii (6.4%), C. famata (2.8%), C. glabrata (1.4%), C. krusei (0.7%) and C. lambica (0.7%). The sources of fungal isolates were blood (47.5%), respiratory tract (17.7%), urinary tract (16.3%), skin and mucous membrane (7.1%), catheter (5.6%), feces (2.1%) and mitral valve tissue (0.7%). The susceptibility test was performed using the methodology of disk-diffusion in agar as recommended in the M44-A2 Document of the Clinical and Laboratory Standards Institute (CLSI). The majority of the clinical isolates (97.2%) was susceptible (S) to fluconazole, although three isolates (2.1%) were susceptible-dose dependent (S-DD) and one of them (0.7%) was resistant (R). The S-DD isolates were C. albicans, C. parapsilosis sensu lato and C. tropicalis. One isolate of C. krusei was resistant to fluconazole. This work documents the high susceptibility to fluconazole by Candida species isolated in Rio de Janeiro, Brazil.
    Keywords Candida ; fluconazole ; antifungal susceptibility ; disk diffusion method ; Microbiology ; QR1-502 ; Science ; Q
    Language English
    Publishing date 2015-06-01T00:00:00Z
    Publisher Sociedade Brasileira de Microbiologia
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Cellular characterisation of Candida tropicalis presenting fluconazole-related trailing growth

    Marcos Dornelas-Ribeiro / Eliane Olmo Pinheiro / Carolina Guerra / Lys Adriana Braga-Silva / Silvia Maia Faria de Carvalho / André Luis Souza dos Santos / Sonia Rozental / Sergio Eduardo Longo Fracalanzza

    Memórias do Instituto Oswaldo Cruz., Vol 107, Iss 1, Pp 31-

    2012  Volume 38

    Abstract: We assessed fluconazole susceptibility in 52 Candida tropicalis clinical strains using seven antifungal susceptibility methods, including broth microdilution (BMD) [standard M27 A3 (with neutral and acid pH), ATB Fungus 3, Vitek 2 system and flow ... ...

    Abstract We assessed fluconazole susceptibility in 52 Candida tropicalis clinical strains using seven antifungal susceptibility methods, including broth microdilution (BMD) [standard M27 A3 (with neutral and acid pH), ATB Fungus 3, Vitek 2 system and flow cytometric analysis] and agar-based methods (disk diffusion and E-test). Trailing growth, detection of cell-associated secreted aspartic proteases (Saps) and morphological and ultrastructural traits of these clinical strains were also examined. The ranges of fluconazole 24 h-minimum inhibitory concentration (MIC) values were similar among all methods. The essential agreement among the methods used for MIC determinations was excellent and all methods categorised all strains as susceptible, except for one strain that showed a minor error. The presence of the trailing effect was assessed by six methods. Trailing positivity was observed for 86.5-100% of the strains. The exception was the BMD-Ac method where trailing growth was not observed. Morphological and ultrastructural alterations were detected in C. tropicalis trailing cells, including mitochondrial swelling and cell walls with irregular shapes. We tested the production of Saps in 13 C. tropicalis strains expressing trailing growth through flow cytometry. Our results showed that all of the C. tropicalis strains up-regulated surface Sap expression after 24 h or 48 h of exposure to fluconazole, which was not observed in untreated yeast strains. We concluded that C. tropicalis strains expressing trailing growth presented some particular features on both biological and ultrastructural levels.
    Keywords Candida tropicalis ; antifungal susceptibility test ; trailing growth ; biological and ultrastructural alterations ; aspartic protease production ; Arctic medicine. Tropical medicine ; RC955-962 ; Microbiology ; QR1-502
    Subject code 610 ; 500
    Language English
    Publishing date 2012-02-01T00:00:00Z
    Publisher Instituto Oswaldo Cruz, Ministério da Saúde
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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