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  1. Article: Comparable Neutralization of the SARS-CoV-2 Omicron BA.1 and BA.2 Variants.

    Yu, Jingyou / Collier, Ai-Ris Y / Rowe, Marjorie / Mardas, Fatima / Ventura, John D / Wan, Huahua / Miller, Jessica / Powers, Olivia / Chung, Benjamin / Siamatu, Mazuba / Hachmann, Nicole P / Surve, Nehalee / Nampanya, Felix / Chandrashekar, Abishek / Barouch, Dan H

    medRxiv : the preprint server for health sciences

    2022  

    Abstract: The SARS-CoV-2 Omicron variant (B.1.1.529) has three major lineages BA.1, BA.2, and BA ... ...

    Abstract The SARS-CoV-2 Omicron variant (B.1.1.529) has three major lineages BA.1, BA.2, and BA.3
    Language English
    Publishing date 2022-02-07
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2022.02.06.22270533
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Neutralization of the SARS-CoV-2 Omicron BA.1 and BA.2 Variants.

    Yu, Jingyou / Collier, Ai-Ris Y / Rowe, Marjorie / Mardas, Fatima / Ventura, John D / Wan, Huahua / Miller, Jessica / Powers, Olivia / Chung, Benjamin / Siamatu, Mazuba / Hachmann, Nicole P / Surve, Nehalee / Nampanya, Felix / Chandrashekar, Abishek / Barouch, Dan H

    The New England journal of medicine

    2022  Volume 386, Issue 16, Page(s) 1579–1580

    MeSH term(s) Antibodies, Neutralizing/immunology ; Antibodies, Neutralizing/therapeutic use ; COVID-19/immunology ; COVID-19/prevention & control ; COVID-19/therapy ; Humans ; SARS-CoV-2/drug effects ; SARS-CoV-2/genetics ; SARS-CoV-2/immunology ; Spike Glycoprotein, Coronavirus
    Chemical Substances Antibodies, Neutralizing ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2022-03-16
    Publishing country United States
    Document type Letter ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 207154-x
    ISSN 1533-4406 ; 0028-4793
    ISSN (online) 1533-4406
    ISSN 0028-4793
    DOI 10.1056/NEJMc2201849
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  3. Article ; Online: Engineering melon plants with improved fruit shelf life using the TILLING approach.

    Dahmani-Mardas, Fatima / Troadec, Christelle / Boualem, Adnane / Lévêque, Sylvie / Alsadon, Abdullah A / Aldoss, Abdullah A / Dogimont, Catherine / Bendahmane, Abdelhafid

    PloS one

    2010  Volume 5, Issue 12, Page(s) e15776

    Abstract: Background: Fruit ripening and softening are key traits that have an effect on food supply, fruit nutritional value and consequently, human health. Since ethylene induces ripening of climacteric fruit, it is one of the main targets to control fruit over ...

    Abstract Background: Fruit ripening and softening are key traits that have an effect on food supply, fruit nutritional value and consequently, human health. Since ethylene induces ripening of climacteric fruit, it is one of the main targets to control fruit over ripening that leads to fruit softening and deterioration. The characterization of the ethylene pathway in Arabidopsis and tomato identified key genes that control fruit ripening.
    Methodology/principal findings: To engineer melon fruit with improved shelf-life, we conducted a translational research experiment. We set up a TILLING platform in a monoecious and climacteric melon line, cloned genes that control ethylene production and screened for induced mutations that lead to fruits with enhanced shelf life. Two missense mutations, L124F and G194D, of the ethylene biosynthetic enzyme, ACC oxidase 1, were identified and the mutant plants were characterized with respect to fruit maturation. The L124F mutation is a conservative mutation occurring away from the enzyme active site and thus was predicted to not affect ethylene production and thus fruit ripening. In contrast, G194D modification occurs in a highly conserved amino acid position predicted, by crystallographic analysis, to affect the enzymatic activity. Phenotypic analysis of the G194D mutant fruit showed complete delayed ripening and yellowing with improved shelf life and, as predicted, the L124F mutation did not have an effect.
    Conclusions/significance: We constructed a mutant collection of 4023 melon M2 families. Based on the TILLING of 11 genes, we calculated the overall mutation rate of one mutation every 573 kb and identified 8 alleles per tilled kilobase. We also identified a TILLING mutant with enhanced fruit shelf life. This work demonstrates the effectiveness of TILLING as a reverse genetics tool to improve crop species. As cucurbits are model species in different areas of plant biology, we anticipate that the developed tool will be widely exploited by the scientific community.
    MeSH term(s) Amino Acid Oxidoreductases/genetics ; Amino Acid Sequence ; Catalytic Domain ; Crystallography, X-Ray/methods ; Cucurbitaceae/genetics ; Cucurbitaceae/physiology ; Ethylenes/chemistry ; Fruit/genetics ; Gene Expression Regulation, Plant ; Genetic Engineering/methods ; Molecular Sequence Data ; Mutation, Missense ; Phenotype ; Protein Conformation ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid
    Chemical Substances Ethylenes ; ethylene (91GW059KN7) ; Amino Acid Oxidoreductases (EC 1.4.-) ; 1-aminocyclopropane-1-carboxylic acid oxidase (EC 1.4.3.-)
    Language English
    Publishing date 2010-12-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0015776
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Engineering Melon Plants with Improved Fruit Shelf Life Using the TILLING Approach

    Dahmani-Mardas, Fatima / Troadec, Christelle / Boualem, Adnane / Leveque, Sylvie / Alsadon, Abdullah A. / Aldoss, Abdullah A. / Dogimont, Catherine / Bendahmane, Abdelhafid

    Plos One 12 (5), . (2010)

    Abstract: Background: Fruit ripening and softening are key traits that have an effect on food supply, fruit nutritional value and consequently, human health. Since ethylene induces ripening of climacteric fruit, it is one of the main targets to control fruit over ... ...

    Abstract Background: Fruit ripening and softening are key traits that have an effect on food supply, fruit nutritional value and consequently, human health. Since ethylene induces ripening of climacteric fruit, it is one of the main targets to control fruit over ripening that leads to fruit softening and deterioration. The characterization of the ethylene pathway in Arabidopsis and tomato identified key genes that control fruit ripening. [br/]Methodology/Principal Findings: To engineer melon fruit with improved shelf-life, we conducted a translational research experiment. We set up a TILLING platform in a monoecious and climacteric melon line, cloned genes that control ethylene production and screened for induced mutations that lead to fruits with enhanced shelf life. Two missense mutations, L124F and G194D, of the ethylene biosynthetic enzyme, ACC oxidase 1, were identified and the mutant plants were characterized with respect to fruit maturation. The L124F mutation is a conservative mutation occurring away from the enzyme active site and thus was predicted to not affect ethylene production and thus fruit ripening. In contrast, G194D modification occurs in a highly conserved amino acid position predicted, by crystallographic analysis, to affect the enzymatic activity. Phenotypic analysis of the G194D mutant fruit showed complete delayed ripening and yellowing with improved shelf life and, as predicted, the L124F mutation did not have an effect. [br/]Conclusions/Significance: We constructed a mutant collection of 4023 melon M2 families. Based on the TILLING of 11 genes, we calculated the overall mutation rate of one mutation every 573 kb and identified 8 alleles per tilled kilobase. We also identified a TILLING mutant with enhanced fruit shelf life. This work demonstrates the effectiveness of TILLING as a reverse genetics tool to improve crop species. As cucurbits are model species in different areas of plant biology, we anticipate that the developed tool will be widely exploited by the scientific community.
    Language English
    Document type Article
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article: Engineering Melon Plants with Improved Fruit Shelf Life Using the TILLING Approach

    Dahmani-Mardas, Fatima / Troadec, Christelle / Boualem, Adnane / Leveque, Sylvie / Alsadon, Abdullah A. / Aldoss, Abdullah A. / Dogimont, Catherine / Bendahmane, Abdelhafid

    Plos One 12 (5), . (2010)

    Abstract: Background: Fruit ripening and softening are key traits that have an effect on food supply, fruit nutritional value and consequently, human health. Since ethylene induces ripening of climacteric fruit, it is one of the main targets to control fruit over ... ...

    Abstract Background: Fruit ripening and softening are key traits that have an effect on food supply, fruit nutritional value and consequently, human health. Since ethylene induces ripening of climacteric fruit, it is one of the main targets to control fruit over ripening that leads to fruit softening and deterioration. The characterization of the ethylene pathway in Arabidopsis and tomato identified key genes that control fruit ripening. [br/]Methodology/Principal Findings: To engineer melon fruit with improved shelf-life, we conducted a translational research experiment. We set up a TILLING platform in a monoecious and climacteric melon line, cloned genes that control ethylene production and screened for induced mutations that lead to fruits with enhanced shelf life. Two missense mutations, L124F and G194D, of the ethylene biosynthetic enzyme, ACC oxidase 1, were identified and the mutant plants were characterized with respect to fruit maturation. The L124F mutation is a conservative mutation occurring away from the enzyme active site and thus was predicted to not affect ethylene production and thus fruit ripening. In contrast, G194D modification occurs in a highly conserved amino acid position predicted, by crystallographic analysis, to affect the enzymatic activity. Phenotypic analysis of the G194D mutant fruit showed complete delayed ripening and yellowing with improved shelf life and, as predicted, the L124F mutation did not have an effect. [br/]Conclusions/Significance: We constructed a mutant collection of 4023 melon M2 families. Based on the TILLING of 11 genes, we calculated the overall mutation rate of one mutation every 573 kb and identified 8 alleles per tilled kilobase. We also identified a TILLING mutant with enhanced fruit shelf life. This work demonstrates the effectiveness of TILLING as a reverse genetics tool to improve crop species. As cucurbits are model species in different areas of plant biology, we anticipate that the developed tool will be widely exploited by the scientific community.
    Language English
    Document type Article
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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