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  1. Article ; Online: Comparative study of in vitro activity of tedizolid and linezolid against Mycobacterium avium complex.

    Marfil, Eduardo / Ruiz, Pilar / Martínez-Martínez, Luis / Causse, Manuel

    Journal of global antimicrobial resistance

    2022  Volume 30, Page(s) 395–398

    Abstract: Objectives: The aim of this study is to compare the in vitro activity and minimal inhibitory concentration (MIC) distributions of tedizolid and linezolid against Mycobacterium avium complex (MAC) strains using a reference broth microdilution assay and a ...

    Abstract Objectives: The aim of this study is to compare the in vitro activity and minimal inhibitory concentration (MIC) distributions of tedizolid and linezolid against Mycobacterium avium complex (MAC) strains using a reference broth microdilution assay and a macrodilution assay with the Bactec-MGIT-960.
    Methods: A total of 37 clinical isolates of MAC were included in the study. Reference broth microdilution was performed according to CLSI guidelines in a range of concentrations from 64 to 0.064 mg/L. Macrodilution was performed with the Bactec-MGIT-960 system. The cut-off points defined by CLSI for linezolid (resistant: > 16 mg/L, intermediate: 16 mg/L, susceptible: <16 mg/L) were used to define clinical categories of this drug. Essential agreement for both linezolid and tedizolid and categorical agreement for linezolid were defined following FDA criteria.
    Results: The MIC
    Conclusions: Tedizolid showed higher in vitro activity than linezolid against the tested MAC isolates. Macrodilution using the BD Bactec-MGIT-960 system is a practical approach to determine the susceptibility of MAC strains to tedizolid.
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Humans ; Linezolid/pharmacology ; Mycobacterium avium Complex ; Mycobacterium avium-intracellulare Infection ; Organophosphates/pharmacology ; Oxazoles/pharmacology ; Oxazolidinones ; Tetrazoles
    Chemical Substances Anti-Bacterial Agents ; Organophosphates ; Oxazoles ; Oxazolidinones ; Tetrazoles ; tedizolid (97HLQ82NGL) ; Linezolid (ISQ9I6J12J)
    Language English
    Publishing date 2022-07-18
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2710046-7
    ISSN 2213-7173 ; 2213-7173
    ISSN (online) 2213-7173
    ISSN 2213-7173
    DOI 10.1016/j.jgar.2022.07.012
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Impact of KPC Production and High-Level Meropenem Resistance on All-Cause Mortality of Ventilator-Associated Pneumonia in Association with Klebsiella pneumoniae.

    Rivera-Espinar, Francisco / Machuca, Isabel / Tejero, Rocio / Rodríguez, Jorge / Mula, Ana / Marfil, Eduardo / Cano, Ángela / Gutiérrez-Gutiérrez, Belén / Rodríguez, Marina / Pozo, Juan Carlos / De la Fuente, Carmen / Rodriguez-Baño, Jesús / Martínez-Martínez, Luis / León, Rafael / Torre-Cisneros, Julian

    Antimicrobial agents and chemotherapy

    2020  Volume 64, Issue 6

    Abstract: Carbapenemase- ... ...

    Abstract Carbapenemase-producing
    MeSH term(s) Anti-Bacterial Agents/therapeutic use ; Bacterial Proteins/genetics ; Humans ; Klebsiella Infections/drug therapy ; Klebsiella pneumoniae ; Meropenem/therapeutic use ; Pneumonia, Ventilator-Associated/drug therapy ; Retrospective Studies ; beta-Lactamases/genetics
    Chemical Substances Anti-Bacterial Agents ; Bacterial Proteins ; beta-Lactamases (EC 3.5.2.6) ; carbapenemase (EC 3.5.2.6) ; Meropenem (FV9J3JU8B1)
    Language English
    Publishing date 2020-05-21
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 217602-6
    ISSN 1098-6596 ; 0066-4804
    ISSN (online) 1098-6596
    ISSN 0066-4804
    DOI 10.1128/AAC.02164-19
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Sample pooling for SARS-CoV-2 RT-PCR screening.

    de Salazar, Adolfo / Aguilera, Antonio / Trastoy, Rocio / Fuentes, Ana / Alados, Juan Carlos / Causse, Manuel / Galán, Juan Carlos / Moreno, Antonio / Trigo, Matilde / Pérez-Ruiz, Mercedes / Roldán, Carolina / Pena, Maria José / Bernal, Samuel / Serrano-Conde, Esther / Barbeito, Gema / Torres, Eva / Riazzo, Cristina / Cortes-Cuevas, Jose Luis / Chueca, Natalia /
    Coira, Amparo / Sanchez-Calvo, Juan Manuel / Marfil, Eduardo / Becerra, Federico / Gude, María José / Pallarés, Ángeles / Pérez Del Molino, María Luisa / García, Federico

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

    2020  Volume 26, Issue 12, Page(s) 1687.e1–1687.e5

    Abstract: Objective: To evaluate the efficacy of sample pooling compared to the individual analysis for the diagnosis of coronavirus disease 2019 (COVID-19) by using different commercial platforms for nucleic acid extraction and amplification.: Methods: A ... ...

    Abstract Objective: To evaluate the efficacy of sample pooling compared to the individual analysis for the diagnosis of coronavirus disease 2019 (COVID-19) by using different commercial platforms for nucleic acid extraction and amplification.
    Methods: A total of 3519 nasopharyngeal samples received at nine Spanish clinical microbiology laboratories were processed individually and in pools (342 pools of ten samples and 11 pools of nine samples) according to the existing methodology in place at each centre.
    Results: We found that 253 pools (2519 samples) were negative and 99 pools (990 samples) were positive; with 241 positive samples (6.85%), our pooling strategy would have saved 2167 PCR tests. For 29 pools (made out of 290 samples), we found discordant results when compared to their correspondent individual samples, as follows: in 22 of 29 pools (28 samples), minor discordances were found; for seven pools (7 samples), we found major discordances. Sensitivity, specificity and positive and negative predictive values for pooling were 97.10% (95% confidence interval (CI), 94.11-98.82), 100%, 100% and 99.79% (95% CI, 99.56-99.90) respectively; accuracy was 99.80% (95% CI, 99.59-99.92), and the kappa concordant coefficient was 0.984. The dilution of samples in our pooling strategy resulted in a median loss of 2.87 (95% CI, 2.46-3.28) cycle threshold (C
    Conclusions: We found a high efficiency of pooling strategies for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA testing across different RNA extraction and amplification platforms, with excellent performance in terms of sensitivity, specificity and positive and negative predictive values.
    MeSH term(s) Biostatistics ; COVID-19/diagnosis ; COVID-19/epidemiology ; COVID-19/virology ; COVID-19 Nucleic Acid Testing/methods ; Humans ; Mass Screening/methods ; Nasopharynx/virology ; RNA, Viral/genetics ; SARS-CoV-2/isolation & purification ; Sensitivity and Specificity ; Spain/epidemiology ; Specimen Handling/methods
    Chemical Substances RNA, Viral
    Keywords covid19
    Language English
    Publishing date 2020-09-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 1328418-6
    ISSN 1469-0691 ; 1470-9465 ; 1198-743X
    ISSN (online) 1469-0691
    ISSN 1470-9465 ; 1198-743X
    DOI 10.1016/j.cmi.2020.09.008
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 4541: Show issues Location:
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  4. Article: Sample pooling for SARS-CoV-2 RT-PCR screening

    de Salazar, Adolfo / Aguilera, Antonio / Trastoy, Rocio / Fuentes, Ana / Alados, Juan Carlos / Causse, Manuel / Galán, Juan Carlos / Moreno, Antonio / Trigo, Matilde / Pérez-Ruiz, Mercedes / Roldán, Carolina / Pena, Maria José / Bernal, Samuel / Serrano-Conde, Esther / Barbeito, Gema / Torres, Eva / Riazzo, Cristina / Cortes-Cuevas, Jose Luis / Chueca, Natalia /
    Coira, Amparo / Sanchez-Calvo, Juan Manuel / Marfil, Eduardo / Becerra, Federico / Gude, María José / Pallarés, Ángeles / Pérez Del Molino, María Luisa / García, Federico

    Clin. microbiol. infect

    Abstract: OBJECTIVE: To evaluate the efficacy of sample pooling compared to the individual analysis for the diagnosis of coronavirus disease 2019 (COVID-19) by using different commercial platforms for nucleic acid extraction and amplification. METHODS: A total of ... ...

    Abstract OBJECTIVE: To evaluate the efficacy of sample pooling compared to the individual analysis for the diagnosis of coronavirus disease 2019 (COVID-19) by using different commercial platforms for nucleic acid extraction and amplification. METHODS: A total of 3519 nasopharyngeal samples received at nine Spanish clinical microbiology laboratories were processed individually and in pools (342 pools of ten samples and 11 pools of nine samples) according to the existing methodology in place at each centre. RESULTS: We found that 253 pools (2519 samples) were negative and 99 pools (990 samples) were positive; with 241 positive samples (6.85%), our pooling strategy would have saved 2167 PCR tests. For 29 pools (made out of 290 samples), we found discordant results when compared to their correspondent individual samples, as follows: in 22 of 29 pools (28 samples), minor discordances were found; for seven pools (7 samples), we found major discordances. Sensitivity, specificity and positive and negative predictive values for pooling were 97.10% (95% confidence interval (CI), 94.11-98.82), 100%, 100% and 99.79% (95% CI, 99.56-99.90) respectively; accuracy was 99.80% (95% CI, 99.59-99.92), and the kappa concordant coefficient was 0.984. The dilution of samples in our pooling strategy resulted in a median loss of 2.87 (95% CI, 2.46-3.28) cycle threshold (Ct) for E gene, 3.36 (95% CI, 2.89-3.85) Ct for the RdRP gene and 2.99 (95% CI, 2.56-3.43) Ct for the N gene. CONCLUSIONS: We found a high efficiency of pooling strategies for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA testing across different RNA extraction and amplification platforms, with excellent performance in terms of sensitivity, specificity and positive and negative predictive values.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #753740
    Database COVID19

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  5. Article ; Online: Sample Pooling as an efficient strategy for SARS-COV-2 RT-PCR screening: a multicenter study in Spain

    de Salazar, Adolfo / Aguilera, Antonio / Trastoy, Rocio / Fuentes, Ana / Alados, Juan Carlos / Causse, Manuel / Galan, Juan Carlos / Moreno, Antonio / Trigo, Matilde / Perez, Mercedes / Roldan, Carolina / Pena, Maria Jose / Bernal, Samuel / Serrano-Conde, Esther / Barbeito, Gema / Torres, Eva / Riazzo, Cristina / Cortes-Cuevas, Jose Luis / Chueca, Natalia /
    Coira, Amparo / Sanchez-Calvo, Juan Manuel / Marfil, Eduardo / Becerra, Federico / Gude, Maria Jose / Pallares, Angeles / Perez del Molino, Maria Luisa / Garcia, Federico

    medRxiv

    Abstract: Importance: The actual demand on SARS-CoV-2 diagnosis is a current challenge for clinical laboratories. Sample pooling may help to ameliorate workload in clinical laboratories. Objective: to evaluate the efficacy of sample pooling compared to the ... ...

    Abstract Importance: The actual demand on SARS-CoV-2 diagnosis is a current challenge for clinical laboratories. Sample pooling may help to ameliorate workload in clinical laboratories. Objective: to evaluate the efficacy of sample pooling compared to the individual analysis for the diagnosis of CoVID-19, by using different commercial platforms for nucleic acid extraction and amplification. Design and settings: observational, prospective, multicentre study across 9 Spanish clinical microbiology laboratories including SARS-CoV-2 RNA testing performed in April 2020, during the first three days after acceptance to participate. Participants and Methods: 3519 naso-oro-pharyngeal samples received at the participating laboratories were processed individually and in pools (351 pools) according to the existing methodology in each of the centres. Results: We found that 253 pools (2519 samples) were negative, and 99 pools (990 samples) were positive; with 241 positive samples (6.85%), our pooling strategy would have saved 2167 PCR tests. For 29 pools (made out of 290 samples) we found discordant results when compared to their correspondent individual samples: in 24/29 pools (30 samples), minor discordances were found; for five pools (5 samples), we found major discordances. Sensitivity, specificity, positive and negative predictive values for pooling were 97.93%, 100%, 100% and 99.85% respectively; accuracy was 99.86% and kappa concordant coefficient was 0.988. As a result of the sample dilution effect of pooling, a loss of 2-3 Cts was observed for E, N or RdRP genes. Conclusion: we show a high efficiency of pooling strategies for SARS-CoV-2 RNA testing, across different RNA extraction and amplification platforms, with excellent performance in terms of sensitivity, specificity, and positive and negative predictive values. We believe that our results may help clinical laboratories to respond to the actual demand and clinical need on SARS-CoV-2 testing, especially for the screening of low prevalence populations.
    Keywords covid19
    Language English
    Publishing date 2020-07-06
    Publisher Cold Spring Harbor Laboratory Press
    Document type Article ; Online
    DOI 10.1101/2020.07.04.20146027
    Database COVID19

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  6. Article ; Online: Sample pooling for SARS-CoV-2 RT-PCR screening

    de Salazar, Adolfo / Aguilera, Antonio / Trastoy, Rocio / Fuentes, Ana / Alados, Juan Carlos / Causse, Manuel / Galán, Juan Carlos / Moreno, Antonio / Trigo, Matilde / Pérez-Ruiz, Mercedes / Roldán, Carolina / Pena, Maria José / Bernal, Samuel / Serrano-Conde, Esther / Barbeito, Gema / Torres, Eva / Riazzo, Cristina / Cortes-Cuevas, Jose Luis / Chueca, Natalia /
    Coira, Amparo / Sanchez-Calvo, Juan Manuel / Marfil, Eduardo / Becerra, Federico / Gude, María José / Pallarés, Ángeles / Pérez Del Molino, María Luisa / García, Federico

    Clinical Microbiology and Infection ; ISSN 1198-743X

    2020  

    Keywords Microbiology (medical) ; Infectious Diseases ; General Medicine ; covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    DOI 10.1016/j.cmi.2020.09.008
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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