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Article ; Online: Umbilical artery Thrombosis: A case report of prenatal diagnosis and systematic review of the literature.

Romani, Eleonora / Marinelli, Laura / Ponziani, Ilaria / Masini, Giulia / Castiglione, Francesca / Nardi, Eleonora / Pasquini, Lucia

European journal of obstetrics, gynecology, and reproductive biology

2024 Volume 296, Page(s) 6–12

Abstract: Umbilical Artery Thrombosis (UAT) is an extremely rare complication of pregnancy strongly associated with severe fetal distress and death. The pathogenesis is still unclear but it is often associated with anatomical cord abnormalities that leads to blood ...

Abstract	Umbilical Artery Thrombosis (UAT) is an extremely rare complication of pregnancy strongly associated with severe fetal distress and death. The pathogenesis is still unclear but it is often associated with anatomical cord abnormalities that leads to blood stasis and thrombosis formation. Other possible risk factors are maternal thrombophilia, autoimmune disease, gestational diabetes, hypertension disorders of pregnancy and Rh-alloimmunization. The most common clinical symptom is the reduction of fetal movements. The diagnosis is histopathological, but it can be suspected by clinical and prenatal ultrasound findings. Generally, the first choice therapy is the immediate delivery with cesarean section. This study reported a case of a spontaneous intrauterine UAT in a low-risk pregnancy and a systematic review of the literature on clinical, ultrasound and histopathological findings of UAT, in order to help clinicians in the diagnostic process and management of this rare complication.
MeSH term(s)	Pregnancy ; Humans ; Female ; Umbilical Arteries/diagnostic imaging ; Cesarean Section/adverse effects ; Pregnancy Complications/pathology ; Prenatal Diagnosis ; Thrombosis/etiology ; Ultrasonography, Prenatal/adverse effects
Language	English
Publishing date	2024-02-10
Publishing country	Ireland
Document type	Systematic Review ; Case Reports ; Journal Article ; Review
ZDB-ID	190605-7
ISSN	1872-7654 ; 0301-2115 ; 0028-2243
ISSN (online)	1872-7654
ISSN	0301-2115 ; 0028-2243
DOI	10.1016/j.ejogrb.2024.02.016
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Book: Ein Wiedersehen mit Liliane

Marinelli, Laura

findet Aschenputtel ihren Traumprinzen? ; Roman

(Fürstenkrone ; 1208)

2015

Author's details	von Laura Marinelli
Series title	Fürstenkrone ; 1208
Language	German
Size	65 S
Publisher	Kelter
Publishing place	Hamburg
Document type	Book
Database	Former special subject collection: coastal and deep sea fishing

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Article ; Online: Does chronic low molecular weight heparins use during pregnancy increase the risk of postpartum hemorrhage?

Huri, Mor / Zullino, Sara / Marinelli, Laura / Clemenza, Sara / Petraglia, Felice / Mecacci, Federico

Thrombosis research

2022 Volume 222, Page(s) 12–15

MeSH term(s)	Pregnancy ; Female ; Humans ; Postpartum Hemorrhage ; Heparin, Low-Molecular-Weight/adverse effects ; Risk Factors
Chemical Substances	Heparin, Low-Molecular-Weight
Language	English
Publishing date	2022-12-18
Publishing country	United States
Document type	Letter
ZDB-ID	121852-9
ISSN	1879-2472 ; 0049-3848
ISSN (online)	1879-2472
ISSN	0049-3848
DOI	10.1016/j.thromres.2022.12.009
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Article: Robotic resection of a single adenoid cystic tumor liver metastasis using ICG fluorescence. A case report and literature review.

Pasquale, Alessio / Marinelli, Laura / Ciarleglio, Francesco Antonio / Campora, Michela / Salimian, Nick / Viel, Giovanni / Brolese, Alberto

Frontiers in surgery

2023 Volume 10, Page(s) 1162639

Abstract: Adenoid cystic carcinoma (AdCC) is a rare tumor that typically develops in the salivary glands and less frequently in other sites of the head and neck region. Only a few cases of resected metachronous liver metastases have been reported. Minimally ... ...

Abstract	Adenoid cystic carcinoma (AdCC) is a rare tumor that typically develops in the salivary glands and less frequently in other sites of the head and neck region. Only a few cases of resected metachronous liver metastases have been reported. Minimally invasive surgery is currently the gold standard of care for liver resections; furthermore, the use of Indocyanine Green (ICG) is continuously increasing in surgical practice, especially in cases of primary liver tumors and colorectal liver metastases, due to its capacity to enhance liver nodules. We report the case of a 54-year-old male with a single liver metastasis of AdCC, located in SIII, who presented in our center 9 months after resection of a primary tumor of the laryngotracheal junction and adjuvant proton therapy. A 25-mg injection of ICG (0.3 mg/kg) was administered 48 h before surgery in order to highlight the tumor and perform an ICG-guided resection. The lesion was clearly visible during surgery, and, given its position and the proximity to the main lobar vessels of the left lobe, we opted for a left lateral sectionectomy. The outcome was unremarkable, with no major postoperative complications. The administration of ICG 48 h before surgery seems to be a valid tool even in cases of AdCC liver metastases, providing surgeons with better visualization of the lesion and improving the precision of the resection.
Language	English
Publishing date	2023-03-23
Publishing country	Switzerland
Document type	Case Reports
ZDB-ID	2773823-1
ISSN	2296-875X
ISSN	2296-875X
DOI	10.3389/fsurg.2023.1162639
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Article ; Online: Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA.

Marinelli, Laura J / Piuri, Mariana / Hatfull, Graham F

Methods in molecular biology (Clifton, N.J.)

2018 Volume 1898, Page(s) 69–80

Abstract: We describe a recombineering-based method for the genetic manipulation of lytically replicating bacteriophages, focusing on mycobacteriophages. The approach utilizes recombineering-proficient strains of Mycobacterium smegmatis and employs a ... ...

Abstract	We describe a recombineering-based method for the genetic manipulation of lytically replicating bacteriophages, focusing on mycobacteriophages. The approach utilizes recombineering-proficient strains of Mycobacterium smegmatis and employs a cotransformation strategy with purified phage genomic DNA and a mutagenic substrate, which selects for only those cells that are competent to take up DNA. The cotransformation method, combined with the high rates of recombination obtained in M. smegmatis recombineering strains, allows for the efficient and rapid generation of bacteriophage mutants.
MeSH term(s)	Bacteriophages/chemistry ; Bacteriophages/genetics ; DNA/chemistry ; DNA/genetics ; Electrochemotherapy ; Electroporation ; Genetic Engineering ; Mutagenesis/genetics ; Mycobacterium smegmatis/genetics ; Mycobacterium smegmatis/virology ; Recombination, Genetic
Chemical Substances	DNA (9007-49-2)
Language	English
Publishing date	2018-12-19
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-4939-8940-9_6
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Fenoldopam Increases Urine Output in Oliguric Critically Ill Surgical Patients.

Cagliani, Joaquin A / Marinelli, Laura / Cho, Youngmin / Miyara, Santiago J / Ruhemann, Andres / Loyola, Andre / Molmenti, Ernesto / Smith, Candace / Coppa, Gene / Barrera, Rafael

Cureus

2021 Volume 13, Issue 12, Page(s) e20445

Abstract: Background Fenoldopam is a short-acting dopamine A1 receptor agonist which mediates vasodilation of the renal arteries, thereby increasing urine output. The objective of this study was to compare the effects of fenoldopam and its synergistic effect on ... ...

Abstract	Background Fenoldopam is a short-acting dopamine A1 receptor agonist which mediates vasodilation of the renal arteries, thereby increasing urine output. The objective of this study was to compare the effects of fenoldopam and its synergistic effect on furosemide for improving the urine output in postoperative critically ill patients with acute kidney injury (AKI). Methods This is a retrospective study of postoperative critically ill patients with AKI. Patients who received furosemide (control group) were compared with those who received furosemide plus fenoldopam (treatment group) and evaluated at 12 and 24 hours post-treatment. Patients with oliguria and AKI were included in the study, while patients with chronic kidney disease (CKD) were excluded. Glomerular filtration rate, serum creatinine, blood pressure, calculated fluid accumulation, fluid intake, urine output, and total fluid output were used as variables to assess the medication effect. Results Of the 126 patients who met the inclusion and exclusion criteria, 87 patients received furosemide alone, and 39 patients received furosemide plus fenoldopam during their first 24 hours of admission to the surgical intensive care unit (SICU). Although not statistically significant, the addition of fenoldopam demonstrated an increase in mean urine output of 1525ml (IQR; 1530-2095) in the first 24 hours (P=0.06). There was also noted an increase in the urine output (p= 0.07) and a decrease in the total fluid accumulation when fenoldopam was co-administered with furosemide when compared to the patients who were only treated with furosemide (p=0.06). There was no significant change in creatinine clearance from baseline in either group. Conclusion Fenoldopam may increase urine output in postoperative critically ill patients with acute kidney injury when administered within the first 24 hours of presentation. Based on our results, fenoldopam appears to have a synergistic effect with furosemide in our study population.
Language	English
Publishing date	2021-12-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.20445
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Article: Recombineering: A powerful tool for modification of bacteriophage genomes.

Marinelli, Laura J / Hatfull, Graham F / Piuri, Mariana

Bacteriophage

2012 Volume 2, Issue 1, Page(s) 5–14

Abstract: Recombineering, a recently developed technique for efficient genetic manipulation of bacteria, is facilitated by phage-derived recombination proteins and has the advantage of using DNA substrates with short regions of homology. This system was first ... ...

Abstract	Recombineering, a recently developed technique for efficient genetic manipulation of bacteria, is facilitated by phage-derived recombination proteins and has the advantage of using DNA substrates with short regions of homology. This system was first developed in E. coli but has since been adapted for use in other bacteria. It is now widely used in a number of different systems for a variety of purposes, and the construction of chromosomal gene knockouts, deletions, insertions, point mutations, as well as in vivo cloning, mutagenesis of bacterial artificial chromosomes and phasmids, and the construction of genomic libraries has been reported. However, these methods also can be effectively applied to the genetic modification of bacteriophage genomes, in both their prophage and lytically growing states. The ever-growing collection of fully sequenced bacteriophages raises more questions than they answer, including the unknown functions of vast numbers of genes with no known homologs and of unknown function. Recombineering of phage genomes is central to addressing these questions, enabling the simple construction of mutants, determination of gene essentiality, and elucidation of gene function. In turn, advances in our understanding of phage genomics should present similar recombineering tools for dissecting a multitude of other genetically naïve bacterial systems.
Language	English
Publishing date	2012-05-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	2682433-4
ISSN	2159-7081 ; 2159-7073
ISSN (online)	2159-7081
ISSN	2159-7073
DOI	10.4161/bact.18778
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Article: Early Amiodarone-Induced Pulmonary Toxicity after Endovascular Aneurysm Repair: A Case Report.

Yoon, Uzung / Marinelli, Laura / Ali, Sayed / Huberfeld, Seymour / Barrera, Rafael / Chang, John B

The International journal of angiology : official publication of the International College of Angiology, Inc

2014 Volume 25, Issue 3, Page(s) 189–192

Abstract: Amiodarone is an antiarrhythmic drug that has been commonly used to treat supraventricular and ventricular arrhythmias. This drug is an iodine-containing compound that tends to accumulate in several organs, including the lungs. Especially, its main ... ...

Abstract	Amiodarone is an antiarrhythmic drug that has been commonly used to treat supraventricular and ventricular arrhythmias. This drug is an iodine-containing compound that tends to accumulate in several organs, including the lungs. Especially, its main metabolically active metabolite desethylamiodarone can adversely affect many organs. A very well-known severe complication of amiodarone therapy is the amiodarone-induced pulmonary toxicity. This article presents the case study of an 82-year-old male patient with acute amiodarone-induced pulmonary toxicity. The patient underwent endovascular aneurysm repair for rapidly increasing abdominal aortic aneurysm. During the postoperative period the patient developed rapid atrial fibrillation and amiodarone therapy was initiated. Subsequently, the patient went into acute respiratory failure and was requiring high supplemental oxygen support and a chest X-ray revealed bilateral pulmonary infiltrates. During the hospital course the patient required mechanical ventilator support. With discontinuation of amiodarone, supportive therapy and steroid treatment patient symptoms significantly improved. Amiodarone-induced pulmonary toxicity must be considered in the differential diagnosis of all patients on the medication with progressive or acute respiratory symptoms. Early discontinuation of amiodarone and aggressive corticosteroid therapy should be considered as a viable treatment strategy.
Language	English
Publishing date	2014-08-19
Publishing country	United States
Document type	Case Reports
ZDB-ID	1144390-x
ISSN	1061-1711
ISSN	1061-1711
DOI	10.1055/s-0034-1387170
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Article ; Online: The role of the comprehensive complication index for the prediction of survival after liver transplantation.

Lai, Quirino / Melandro, Fabio / Nowak, Greg / Nicolini, Daniele / Iesari, Samuele / Fasolo, Elisa / Mennini, Gianluca / Romano, Antonio / Mocchegiani, Federico / Ackenine, Kevin / Polacco, Marina / Marinelli, Laura / Ciccarelli, Olga / Zanus, Giacomo / Vivarelli, Marco / Cillo, Umberto / Rossi, Massimo / Ericzon, Bo-Göran / Lerut, Jan

Updates in surgery

2020 Volume 73, Issue 1, Page(s) 209–221

Abstract: In the last years, several scoring systems based on pre- and post-transplant parameters have been developed to predict early post-LT graft function. However, some of them showed poor diagnostic abilities. This study aims to evaluate the role of the ... ...

Abstract	In the last years, several scoring systems based on pre- and post-transplant parameters have been developed to predict early post-LT graft function. However, some of them showed poor diagnostic abilities. This study aims to evaluate the role of the comprehensive complication index (CCI) as a useful scoring system for accurately predicting 90-day and 1-year graft loss after liver transplantation. A training set (n = 1262) and a validation set (n = 520) were obtained. The study was registered at https://www.ClinicalTrials.gov (ID: NCT03723317). CCI exhibited the best diagnostic performance for 90 days in the training (AUC = 0.94; p < 0.001) and Validation Sets (AUC = 0.77; p < 0.001) when compared to the BAR, D-MELD, MELD, and EAD scores. The cut-off value of 47.3 (third quartile) showed a diagnostic odds ratio of 48.3 and 7.0 in the two sets, respectively. As for 1-year graft loss, CCI showed good performances in the training (AUC = 0.88; p < 0.001) and validation sets (AUC = 0.75; p < 0.001). The threshold of 47.3 showed a diagnostic odds ratio of 21.0 and 5.4 in the two sets, respectively. All the other tested scores always showed AUCs < 0.70 in both the sets. CCI showed a good stratification ability in terms of graft loss rates in both the sets (log-rank p < 0.001). In the patients exceeding the CCI ninth decile, 1-year graft survival rates were only 0.7% and 23.1% in training and validation sets, respectively. CCI shows a very good diagnostic power for 90-day and 1-year graft loss in different sets of patients, indicating better accuracy with respect to other pre- and post-LT scores.Clinical Trial Notification: NCT03723317.
MeSH term(s)	Adult ; End Stage Liver Disease/surgery ; Female ; Graft Rejection/diagnosis ; Graft Survival ; Humans ; Liver Transplantation ; Male ; Middle Aged ; Predictive Value of Tests ; Primary Graft Dysfunction/diagnosis ; Research Design ; Time Factors
Language	English
Publishing date	2020-09-06
Publishing country	Italy
Document type	Journal Article
ZDB-ID	2572692-4
ISSN	2038-3312 ; 2038-131X
ISSN (online)	2038-3312
ISSN	2038-131X
DOI	10.1007/s13304-020-00878-4
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Article ; Online: Functional requirements for bacteriophage growth: gene essentiality and expression in mycobacteriophage Giles.

Dedrick, Rebekah M / Marinelli, Laura J / Newton, Gerald L / Pogliano, Kit / Pogliano, Joseph / Hatfull, Graham F

Molecular microbiology

2013 Volume 88, Issue 3, Page(s) 577–589

Abstract: Bacteriophages represent a majority of all life forms, and the vast, dynamic population with early origins is reflected in their enormous genetic diversity. A large number of bacteriophage genomes have been sequenced. They are replete with novel genes ... ...

Abstract	Bacteriophages represent a majority of all life forms, and the vast, dynamic population with early origins is reflected in their enormous genetic diversity. A large number of bacteriophage genomes have been sequenced. They are replete with novel genes without known relatives. We know little about their functions, which genes are required for lytic growth, and how they are expressed. Furthermore, the diversity is such that even genes with required functions - such as virion proteins and repressors - cannot always be recognized. Here we describe a functional genomic dissection of mycobacteriophage Giles, in which the virion proteins are identified, genes required for lytic growth are determined, the repressor is identified, and the transcription patterns determined. We find that although all of the predicted phage genes are expressed either in lysogeny or in lytic growth, 45% of the predicted genes are non-essential for lytic growth. We also describe genes required for DNA replication, show that recombination is required for lytic growth, and that Giles encodes a novel repressor. RNAseq analysis reveals abundant expression of a small non-coding RNA in a lysogen and in late lytic growth, although it is non-essential for lytic growth and does not alter lysogeny.
MeSH term(s)	Chromatography, Liquid ; DNA Replication ; Gene Deletion ; Gene Expression Regulation, Viral ; Genes, Essential ; Genome, Viral ; Lysogeny ; Mycobacteriophages/genetics ; Mycobacteriophages/physiology ; Mycobacterium smegmatis/virology ; Promoter Regions, Genetic ; RNA, Small Untranslated/genetics ; Repressor Proteins ; Tandem Mass Spectrometry ; Transcription, Genetic ; Viral Proteins/genetics ; Virus Replication
Chemical Substances	RNA, Small Untranslated ; Repressor Proteins ; Viral Proteins
Language	English
Publishing date	2013-04-08
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	619315-8
ISSN	1365-2958 ; 0950-382X
ISSN (online)	1365-2958
ISSN	0950-382X
DOI	10.1111/mmi.12210
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