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Article ; Online: Targeted Multiresidue Method for the Analysis of Different Classes of Pesticides in Agro-Food Industrial Sludge by Liquid Chromatography Tandem Mass Spectrometry.

Maragou, Niki C / Balayiannis, George / Karanasios, Evangelos / Markellou, Emilia / Liapis, Konstantinos

2021 Volume 26, Issue 22

Abstract: Sludge generated after washing of fruits and vegetables during agro-food processes is a complex matrix and selective methods for the identification and quantification of pesticides' residues are necessary in order to achieve a sustainable and effective ... ...

Abstract	Sludge generated after washing of fruits and vegetables during agro-food processes is a complex matrix and selective methods for the identification and quantification of pesticides' residues are necessary in order to achieve a sustainable and effective management of the total sewage. The present work describes the development and validation of a reliable, simple and fast analytical method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the determination of 47 pesticides of different chemical classes, including organosphosphates, pyrethroids, neonicotinoids, triazoles and others, in sludge samples after QuEChERS sample preparation. The necessity of the individual steps of QuEChERS was investigated and the LC-ESI-MS/MS conditions were optimized to achieve maximum sensitivity of the target analytes. The method limits of detection (LODs) ranged between 0.0005 mg/kg (imidacloprid) and 0.05 mg/kg (beta cyfluthrin). The recoveries ranged between 71-120% and the repeatability of the method was ≤25% expressed as relative standard deviation. The method was applied to sludge samples generated after washing of fruits in an agro-fruit-packaging unit in Greece. The results showed the presence of 37 pesticides' active substances with concentrations ranging from low ppbs, such as fludioxinil (5 μg/kg) up to low ppms such as beta cyfluthrin (3.5 mg/kg) and with their sum concentration reaching up to 19 mg/kg.
MeSH term(s)	Chromatography, Liquid/methods ; Limit of Detection ; Pesticides/analysis ; Reproducibility of Results ; Sewage/analysis ; Solid Phase Extraction/methods ; Tandem Mass Spectrometry/methods
Chemical Substances	Pesticides ; Sewage
Language	English
Publishing date	2021-11-15
Publishing country	Switzerland
Document type	Journal Article ; Validation Study
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules26226888
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Article: Novel Pilot-Scale Photocatalytic Nanofiltration Reactor for Agricultural Wastewater Treatment.

Theodorakopoulos, George V / Arfanis, Michalis K / Sánchez Pérez, José Antonio / Agüera, Ana / Cadena Aponte, Flor Ximena / Markellou, Emilia / Romanos, George Em / Falaras, Polycarpos

Membranes

2023 Volume 13, Issue 2

Abstract: Nowadays, the increased agro-industrial activities and the inability of traditional wastewater treatment plants (WWTPs) to eliminate recalcitrant organic contaminants are raising a potential worldwide risk for the environment. Among the various advanced ... ...

Abstract	Nowadays, the increased agro-industrial activities and the inability of traditional wastewater treatment plants (WWTPs) to eliminate recalcitrant organic contaminants are raising a potential worldwide risk for the environment. Among the various advanced water treatment technologies that are lately proposed for addressing this challenge, the development and optimization of an innovative hybrid photocatalytic nanofiltration reactor (PNFR) prototype emerges as a prominent solution that achieves synergistic beneficial effects between the photocatalytic degradation activity and size exclusion capacity for micropollutant molecules. Both these features can be contemporarily endued to a multitude of membrane monoliths. The physicochemical and the photoinduced decontamination properties of the titania materials were firstly determined in the powder form, and subsequently, the structural and morphological characterization of the obtained titania-modified membrane monoliths were accomplished. The PNFR unit can operate at high water recovery and low pressures, exhibiting promising removal efficiencies against Acetamiprid (ACT) and Thiabendazole (TBZ) pesticides and achieving the recycling of 15 m
Language	English
Publishing date	2023-02-06
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2614641-1
ISSN	2077-0375
ISSN	2077-0375
DOI	10.3390/membranes13020202
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Article ; Online: Monitoring of glyphosate and AMPA in soil samples from two olive cultivation areas in Greece: aspects related to spray operators activities.

Karanasios, Evangelos / Karasali, Helen / Marousopoulou, Anna / Akrivou, Antigoni / Markellou, Emilia

Environmental monitoring and assessment

2018 Volume 190, Issue 6, Page(s) 361

Abstract: The persistence of glyphosate and its primary metabolite AMPA (aminomethylphosphonic acid) was monitored in two areas in Southern Greece (Peza, Crete and Chora Trifilias, Peloponnese) with a known history of glyphosate use, and the levels of residues ... ...

Abstract	The persistence of glyphosate and its primary metabolite AMPA (aminomethylphosphonic acid) was monitored in two areas in Southern Greece (Peza, Crete and Chora Trifilias, Peloponnese) with a known history of glyphosate use, and the levels of residues were linked to spray operators' activities in the respective areas. A total of 170 samples were collected and analysed from both areas during a 3-year monitoring study. A new method (Impact Assessment Procedure - IAP) designed to assess potential impacts to the environment caused by growers' activities, was utilised in the explanation of the results. The level of residues was compared to the predicted environmental concentrations in soil. The ratio of the measured concentrations to the predicted environmental concentrations (MCs/PECs) was > 1 in Chora the first 2 years of sampling and < 1 in the third year, whilst the MCs/PECs ratio was < 1 in Peza, throughout the whole monitoring period. The compliance to the instructions for best handling practices, which operators received during the monitoring period, was reflected in the amount of residues and the MCs/PECs ratio in the second and especially the third sampling year. Differences in the level of residues between areas as well as sampling sites of the same area were identified. AMPA persisted longer than the parent compound glyphosate in both areas.
MeSH term(s)	Agriculture ; Environmental Monitoring ; Glycine/analogs & derivatives ; Glycine/analysis ; Greece ; Herbicides/analysis ; Olea ; Soil/chemistry ; Soil Pollutants/analysis ; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/analysis ; Glyphosate
Chemical Substances	Herbicides ; Soil ; Soil Pollutants ; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (77521-29-0) ; Glycine (TE7660XO1C)
Language	English
Publishing date	2018-05-25
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	782621-7
ISSN	1573-2959 ; 0167-6369
ISSN (online)	1573-2959
ISSN	0167-6369
DOI	10.1007/s10661-018-6728-x
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Article: Genomic Analysis and Secondary Metabolites Production of the Endophytic Bacillus velezensis Bvel1: A Biocontrol Agent against Botrytis cinerea Causing Bunch Rot in Post-Harvest Table Grapes

Nifakos, Kallimachos / Tsalgatidou, Polina C. / Thomloudi, Eirini-Evangelia / Skagia, Aggeliki / Kotopoulis, Dimitrios / Baira, Eirini / Delis, Costas / Papadimitriou, Konstantinos / Markellou, Emilia / Venieraki, Anastasia / Katinakis, Panagiotis

Plants. 2021 Aug. 20, v. 10, no. 8

2021

Abstract: Botrytis bunch rot caused by Botrytis cinerea is one of the most economically significant post-harvest diseases of grapes. In the present study, we showed that the bacterial strain Bvel1 is phylogenetically affiliated to Bacillus velezensis species. The ... ...

Abstract	Botrytis bunch rot caused by Botrytis cinerea is one of the most economically significant post-harvest diseases of grapes. In the present study, we showed that the bacterial strain Bvel1 is phylogenetically affiliated to Bacillus velezensis species. The strain Bvel1 and its secreted metabolites exerted an antifungal activity, under in vitro conditions, against B. cinerea. UHPLC–HRMS chemical analysis revealed that iturin A2, surfactin-C13 and -C15, oxydifficidin, bacillibactin, L-dihydroanticapsin, and azelaic acid were among the metabolites secreted by Bvel1. Treatment of wounded grape berries with Bacillus sp. Bvel1 cell culture was effective for controlling grey mold ingress and expansion in vivo. The effectiveness of this biological control agent was a function of the cell culture concentration of the antagonist applied, while preventive treatment proved to be more effective compared to curative. The strain Bvel1 exhibited an adequate colonization efficiency in wounded grapes. The whole-genome phylogeny, combined with ANI and dDDH analyses, provided compelling evidence that the strain Bvel1 should be taxonomically classified as Bacillus velezensis. Genome mining approaches showed that the strain Bvel1 harbors 13 antimicrobial biosynthetic gene clusters, including iturin A, fengycin, surfactin, bacilysin, difficidin, bacillaene, and bacillibactin. The results provide new insights into the understanding of the endophytic Bacillus velezensis Bvel1 biocontrol mechanism against post-harvest fungal pathogens, including bunch rot disease in grape berries.
Keywords	Bacillus velezensis ; Botrytis cinerea ; antagonists ; antifungal properties ; biological control ; biological control agents ; biosynthesis ; cell culture ; chemical analysis ; endophytes ; fungi ; gray mold ; iturin ; phylogeny ; secondary metabolites ; surfactin
Language	English
Dates of publication	2021-0820
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants10081716
Database	NAL-Catalogue (AGRICOLA)

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Article: Breeding Bread-Making Wheat Varieties for Organic Farming Systems: The Need to Target Productivity, Robustness, Resource Use Efficiency and Grain Quality Traits.

Rempelos, Leonidas / Wang, Juan / Sufar, Enas Khalid / Almuayrifi, Mohammed Saleh Bady / Knutt, Daryl / Leifert, Halima / Leifert, Alice / Wilkinson, Andrew / Shotton, Peter / Hasanaliyeva, Gultekin / Bilsborrow, Paul / Wilcockson, Steve / Volakakis, Nikolaos / Markellou, Emilia / Zhao, Bingqiang / Jones, Stephen / Iversen, Per Ole / Leifert, Carlo

Foods (Basel, Switzerland)

2023 Volume 12, Issue 6

Abstract: Agronomic protocols (rotation, tillage, fertilization and crop protection) commonly used in organic and conventional crop production differ significantly and there is evidence that modern varieties developed for conventional high-input farming systems do ...

Abstract	Agronomic protocols (rotation, tillage, fertilization and crop protection) commonly used in organic and conventional crop production differ significantly and there is evidence that modern varieties developed for conventional high-input farming systems do not have the combination of traits required for optimum performance in organic farming systems. Specifically, there is evidence that prohibition on the use of water-soluble, mineral N, P and K fertilizers and synthetic pesticide inputs in organic farming results in a need to revise both breeding and selection protocols. For organic production systems, the focus needs to be on the following: (i) traits prioritized by organic farmers such as high nutrient use efficiency from organic fertilizer inputs, competitiveness against weeds, and pest and disease resistance, (ii) processing quality parameters defined by millers and bakers and (iii) nutritional quality parameters demanded by organic consumers. In this article, we review evidence from variety trials and factorial field experiments that (i) studied to what extent there is a need for organic farming focused breeding programs, (ii) investigated which traits/trait combinations should be targeted in these breeding programs and/or (iii) compared the performance of modern varieties developed for the conventional sector with traditional/older varieties favored by organic farmers and/or new varieties developed in organic farming focused breeding programs. Our review focuses on wheat because there have been organic and/or low-input farming focused wheat breeding programs for more than 20 years in Europe, which has allowed the performance of varieties/genotypes from organic/low-input and conventional farming focused breeding programs to be compared.
Language	English
Publishing date	2023-03-13
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods12061209
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Article: Evaluation of plant protection efficacy in field conditions and side effects of Lysobacter capsici AZ78, a biocontrol agent of Plasmopara viticola

Markellou, Emilia / Kapaxidi, Eleftheria / Karamaouna, Filitsa / Samara, Maria / Kyriakopoulou, Katerina / Anastasiadou, Pelagia / Vavoulidou, Evangelia / Meidanis, Marios / Machera, Kyriaki / Mandoulaki, Athanasia / Margaritopoulou, Theoni / Giovannini, Oscar / Tomada, Selena / Pertot, Ilaria / Puopolo, Gerardo

Biocontrol science and technology. 2022 Aug. 03, v. 32, no. 8

2022

Abstract: Downy mildew, caused by Plasmopara viticola, is a highly significant grapevine disease causing severe yield losses and affecting wine quality. Disease control mainly relies on use of copper and synthetic fungicides. Today there is a growing interest in ... ...

Abstract	Downy mildew, caused by Plasmopara viticola, is a highly significant grapevine disease causing severe yield losses and affecting wine quality. Disease control mainly relies on use of copper and synthetic fungicides. Today there is a growing interest in developing microbial biopesticides for downy mildew control in integrated and organic viticulture. Here, the bacterial biocontrol agent Lysobacter capsici AZ78 (AZ78) was tested following a holistic approach including in planta efficacy test (large-scale trial) combined with bioassays aiming to assess potential adverse effects on non-target organisms: beneficial arthropods (Aphidius rhopalosiphi and Typhlodromus pyri), the crustacean Daphnia magna, the algae Selenastrum capricornutum and soil organisms [earthworm Eisenia (Ei.) fetida and worm Enchytraeid (En.) albidus]. Moreover, side effects of AZ78 treatments on grape quality and wine-making process were assessed. The results showed that AZ78 was equally effective to reference fungicide (Kocide®2000) in controlling downy mildew on leaves and bunches. AZ78 had no significant effect on the mortality of the indicator arthropod species at a Limit test Rate, which was equal to four times the recommended field rate. Additionally, no significant effects (mortality, growth rate or reproduction) were observed on D. magna, Ei. albidus, En. fetida and S. capricornutum, indicating AZ78 low toxicity on non-target organisms. Likewise, the yeast population dynamics in fermenting musts was not affected, without compromising the fermentation processes and wine quality. Overall, this study provides new insights into the characterisation of the biocontrol agent AZ78 showing that this bacterium has the potential to be further developed as a commercial biopesticide.
Keywords	Aphidius rhopalosiphi ; Daphnia magna ; Enchytraeidae ; Lysobacter capsici ; Plasmopara viticola ; Selenastrum capricornutum ; Typhlodromus pyri ; Vitis ; bacteria ; biological control ; biological control agents ; copper ; disease control ; downy mildew ; earthworms ; efficacy testing ; fermentation ; fungicides ; grapes ; mortality ; plant protection ; population dynamics ; reproduction ; soil ; toxicity ; viticulture ; wine quality ; winemaking ; yeasts
Language	English
Dates of publication	2022-0803
Size	p. 930-951.
Publishing place	Taylor & Francis
Document type	Article
ZDB-ID	1084478-8
ISSN	1360-0478 ; 0958-3157
ISSN (online)	1360-0478
ISSN	0958-3157
DOI	10.1080/09583157.2022.2064431
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Reynoutria sachalinensis extract elicits SA-dependent defense responses in courgette genotypes against powdery mildew caused by Podosphaera xanthii.

Margaritopoulou, Theoni / Toufexi, Eleftheria / Kizis, Dimosthenis / Balayiannis, George / Anagnostopoulos, Christos / Theocharis, Andreas / Rempelos, Leonidas / Troyanos, Yerasimos / Leifert, Carlo / Markellou, Emilia

Scientific reports

2020 Volume 10, Issue 1, Page(s) 3354

Abstract: Powdery mildew (PM) caused by Podosphaera xanthii is one of the most important courgette diseases with high yield losses and is currently controlled by fungicides and sulphur applications in conventional and organic production. Plant derived elicitors/ ... ...

Abstract	Powdery mildew (PM) caused by Podosphaera xanthii is one of the most important courgette diseases with high yield losses and is currently controlled by fungicides and sulphur applications in conventional and organic production. Plant derived elicitors/inducers of resistance are natural compounds that induce resistance to pathogen attack and promote a faster and/or more robust activation of plant defense responses. Giant knotweed (Reynoutria sachalinensis, RS) extract is a known elicitor of plant defenses but its mode of action remains elusive. The aim of this study was to investigate the mechanisms of foliar RS applications and how these affect PM severity and crop performance when used alone or in combination with genetic resistance. RS foliar treatments significantly reduced conidial germination and PM severity on both an intermediate resistance (IR) and a susceptible (S) genotype. RS application triggered plant defense responses, which induced the formation of callose papillae, hydrogen peroxide accumulation and the Salicylic acid (SA) - dependent pathway. Increased SA production was detected along with increased p-coumaric and caffeic acid concentrations. These findings clearly indicate that RS elicits plant defenses notably as a consequence of SA pathway induction.
MeSH term(s)	Ascomycota/pathogenicity ; Cucurbita/drug effects ; Cucurbita/genetics ; Cucurbita/growth & development ; Cucurbita/microbiology ; Fungicides, Industrial/pharmacology ; Genotype ; Glucans/metabolism ; Hydrogen Peroxide/metabolism ; Plant Diseases/genetics ; Plant Diseases/microbiology ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Polygonum/chemistry ; Salicylic Acid/metabolism
Chemical Substances	Fungicides, Industrial ; Glucans ; Plant Extracts ; callose (9064-51-1) ; Hydrogen Peroxide (BBX060AN9V) ; Salicylic Acid (O414PZ4LPZ)
Language	English
Publishing date	2020-02-25
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-60148-6
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Article ; Online: Breeding Bread-Making Wheat Varieties for Organic Farming Systems: The Need to Target Productivity, Robustness, Resource Use Efficiency and Grain Quality Traits

Foods. 2023 Mar. 13, v. 12, no. 6

2023

Abstract	Agronomic protocols (rotation, tillage, fertilization and crop protection) commonly used in organic and conventional crop production differ significantly and there is evidence that modern varieties developed for conventional high-input farming systems do not have the combination of traits required for optimum performance in organic farming systems. Specifically, there is evidence that prohibition on the use of water-soluble, mineral N, P and K fertilizers and synthetic pesticide inputs in organic farming results in a need to revise both breeding and selection protocols. For organic production systems, the focus needs to be on the following: (i) traits prioritized by organic farmers such as high nutrient use efficiency from organic fertilizer inputs, competitiveness against weeds, and pest and disease resistance, (ii) processing quality parameters defined by millers and bakers and (iii) nutritional quality parameters demanded by organic consumers. In this article, we review evidence from variety trials and factorial field experiments that (i) studied to what extent there is a need for organic farming focused breeding programs, (ii) investigated which traits/trait combinations should be targeted in these breeding programs and/or (iii) compared the performance of modern varieties developed for the conventional sector with traditional/older varieties favored by organic farmers and/or new varieties developed in organic farming focused breeding programs. Our review focuses on wheat because there have been organic and/or low-input farming focused wheat breeding programs for more than 20 years in Europe, which has allowed the performance of varieties/genotypes from organic/low-input and conventional farming focused breeding programs to be compared.
Keywords	breadmaking ; crop production ; disease resistance ; grain quality ; nutrient use efficiency ; nutritive value ; organic fertilizers ; organic production ; pesticides ; pests ; plant protection ; tillage ; water solubility ; wheat ; Europe
Language	English
Dates of publication	2023-0313
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods12061209
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Article: Genomic Analysis and Secondary Metabolites Production of the Endophytic

Nifakos, Kallimachos / Tsalgatidou, Polina C / Thomloudi, Eirini-Evangelia / Skagia, Aggeliki / Kotopoulis, Dimitrios / Baira, Eirini / Delis, Costas / Papadimitriou, Konstantinos / Markellou, Emilia / Venieraki, Anastasia / Katinakis, Panagiotis

Plants (Basel, Switzerland)

2021 Volume 10, Issue 8

Abstract: Botrytis bunch rot caused ... ...

Abstract	Botrytis bunch rot caused by
Language	English
Publishing date	2021-08-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants10081716
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Article: Effect of Organic and Conventional Production Methods on Fruit Yield and Nutritional Quality Parameters in Three Traditional Cretan Grape Varieties: Results from a Farm Survey.

Hasanaliyeva, Gultakin / Chatzidimitrou, Eleni / Wang, Juan / Baranski, Marcin / Volakakis, Nikolaos / Pakos, Panagiotis / Seal, Chris / Rosa, Eduardo A S / Markellou, Emilia / Iversen, Per Ole / Vigar, Vanessa / Willson, Adam / Barkla, Bronwyn / Leifert, Carlo / Rempelos, Leonidas

Foods (Basel, Switzerland)

2021 Volume 10, Issue 2

Abstract: The antioxidants found in grapes and wine have been linked to health benefits in humans, but may be affected by agronomic parameters, grape type/variety, and processing. Here, we report results of a farm survey which investigated the effects of ... ...

Abstract	The antioxidants found in grapes and wine have been linked to health benefits in humans, but may be affected by agronomic parameters, grape type/variety, and processing. Here, we report results of a farm survey which investigated the effects of production system (organic vs. conventional) and grape variety on fruit yield, total antioxidant activity/capacity (TAA, assessed by the Trolox Equivalent Antioxidant Capacity (TEAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays), and total concentrations of total phenolics (TPC) and anthocyanins (TAC) in grapes of one red (Kotsifali) and two white (Villana and/or Vidiano) traditional Cretan grape varieties. Analysis of variance (ANOVA) results showed that grape variety choice had a more substantial effect on TPC, TAA, and TAC than primary production protocols, and significant interactions were identified between production system and grape variety choice for TAA
Language	English
Publishing date	2021-02-22
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods10020476
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