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  1. Article ; Online: Morphological characteristics of Greek Strawberry tree (Arbutus andrachne L.) genotypes

    Markovski Aleksandar

    Acta Agriculturae Serbica, Vol 22, Iss 44, Pp 193-

    2017  Volume 206

    Abstract: The morphological characteristics of fruits and leaves of ten Greek Strawberry tree (Arbutus andrachne L.) genotypes located on the middle course, from the left side of Konjska River, in South part of Republic of Macedonia are investigated. Among the ... ...

    Abstract The morphological characteristics of fruits and leaves of ten Greek Strawberry tree (Arbutus andrachne L.) genotypes located on the middle course, from the left side of Konjska River, in South part of Republic of Macedonia are investigated. Among the investigated characteristics of Greek Strawberry tree genotypes it is noted a large level of polymorphism. The genotype 2 is characterized with twice as big fruit mass (1.87 g) as most of other investigated genotypes. The genotypes 2, 7 and 9 are included in the group with large fruits. According to CIELab color system the fruit skin of genotype 2 is characterized with unusually dark red coloration (L* 21.02; a* 18.8; b* 12.0), while the most attractive are the fruits from the genotype 9 (L* 23.9; a* 28.3; b* 17.4). The investigated genotypes show differences in the fruit anatomy, especially the genotype 7 which is characterized with significantly higher value for lobedness degree, pericarp area and pericarp thickness, percent of pulp and number of seeds in fruit. Large differences in the leaves dimension and form are also determined.
    Keywords Arbutus andrachne L ; genotype ; fruit ; leaf ; polymorphism ; Agriculture ; S
    Subject code 571
    Language English
    Publishing date 2017-01-01T00:00:00Z
    Publisher University of Kragujevac, Faculty of Agronomy, Cacak
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Application of High Performance Liquid Chromatography to the Analysis of Pesticide Residues in Apple Juice

    Velkoska-Markovska Lenche / Petanovska-Ilievska Biljana / Markovski Aleksandar

    Contemporary Agriculture, Vol 67, Iss 1, Pp 93-

    2018  Volume 102

    Abstract: The modern apple production involves the use of large amounts of pesticides that can be found in processed products such as apple juice. Harmful effects of pesticide residues on humans, especially children, are well known, hence the content of pesticide ... ...

    Abstract The modern apple production involves the use of large amounts of pesticides that can be found in processed products such as apple juice. Harmful effects of pesticide residues on humans, especially children, are well known, hence the content of pesticide residues in fruit, vegetables and their juices should be controlled. This study presents an application of a new, relatively simple and reliable analytical method for qualitative and quantitative determination of three organophosphorus and one organonitrogen pesticide residues in apple juices. The analysis utilizes reversed-phase high-performance liquid chromatography (RP-HPLC) followed by UV diode array detection. Prior to HPLC analysis, a solid-phase extraction (SPE) was used for analytes concentration and sample clean-up. Specificity, selectivity, linearity, precision, accuracy and limit of quantification (LOQ) were examined to assess the validity of the developed method. The method had satisfactory values of multiple correlation coefficients for calibration curves (R2 ≥ 0.95 ). The precision was evaluated for the retention times and peak areas, and the estimated values for relative standard deviations (RSD) were 0.05 % - 0.18 % and 0.09 % - 0.62 %, respectively, which indicated an excellent precision of the proposed method. Under the established conditions, the recovery of analytes was 93.80 % - 119.41 %, with relative standard deviations below 0.56 %. This method was successfully applied for determination of some organophosphorus and organonitrogen pesticide residues in apple juices which were taken from Macedonian markets. The achieved values for LOQs were low enough compared to the MRLs of the investigated pesticides in apple according to the Regulation (EC) No 396/2005. Detectable residues of the examined pesticides were not found in the analyzed samples.
    Keywords rp-hplc method ; solid-phase extraction ; pesticide residues ; apple juice ; Agriculture ; S
    Subject code 540
    Language English
    Publishing date 2018-03-01T00:00:00Z
    Publisher Sciendo
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Investigation of the morphometric characteristics of jujube types (Zizyphus jujuba Mill.) fruits in Republic of Macedonia

    Markovski Aleksandar / Velkoska-Markovska Lence

    Genetika, Vol 47, Iss 1, Pp 33-

    2015  Volume 43

    Abstract: In the period 2002-2004 have been studied 131 Jujube types in the experimental orchards of Agricultural Institute in Skopje. They are progeny derived from six varieties of Jujube: Zu tao czao, Da baj czao, Kitajski 2A, Wild midleasiatic jujube type, Ja ... ...

    Abstract In the period 2002-2004 have been studied 131 Jujube types in the experimental orchards of Agricultural Institute in Skopje. They are progeny derived from six varieties of Jujube: Zu tao czao, Da baj czao, Kitajski 2A, Wild midleasiatic jujube type, Ja czao, Vahshski 45-2. The seeds of these varieties were obtained by open pollination. Studies were done on the 7-9 aged plants in full fertility. Characteristics of fruits, fruit dimensions and mass of fruit were determined. It was established that the variety Kitajski 2A has the largest fruits (13.1 g). The progeny of the same variety (Kitajski 2A) is characterized with the largest fruits (8.9 g), and the progeny of the variety with the smallest fruits between varity-mothers - Da baj czao is characterized with the smallest fruits (6.3 g). The type 21/6 has an average the largest fruits (22.2 g), while the smallest are the fruits of the type 16/4 (1.8 g). The fruits from type 21/6 are bigger than fruits of the biggest Jujube variety introduced in R Macedonia -Ta jan czao.
    Keywords jujube ; fruit ; morphometric characteristics ; open pollination ; variety ; types ; Genetics ; QH426-470 ; Biology (General) ; QH301-705.5 ; Science ; Q
    Subject code 333
    Language English
    Publishing date 2015-01-01T00:00:00Z
    Publisher Serbian Genetics Society
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Selective Silencing of Disease-Associated B Lymphocytes from Hashimoto's Thyroiditis Patients by Chimeric Protein Molecules.

    Ralchev, Nikola Ralchev / Markovski, Aleksandar Mishel / Yankova, Inna Angelova / Manoylov, Iliyan Konstantinov / Doytchinova, Irini Atanas / Mihaylova, Nikolina Mihaylova / Shinkov, Alexander Dimitrov / Tchorbanov, Andrey Ivanov

    International journal of molecular sciences

    2022  Volume 23, Issue 23

    Abstract: Hashimoto's thyroiditis is one of the most common endocrine disorders, affecting up to 20% of the adult population. No treatment or prevention exists except hormonal substitution for hypothyroidism. We hypothesize that it may be possible to selectively ... ...

    Abstract Hashimoto's thyroiditis is one of the most common endocrine disorders, affecting up to 20% of the adult population. No treatment or prevention exists except hormonal substitution for hypothyroidism. We hypothesize that it may be possible to selectively suppress anti-thyroglobulin (Tg) IgG antibody-producing B lymphocytes from HT patients by a chimeric protein molecule containing a monoclonal antibody specific for the human inhibitory receptor CR1, coupled to peptide epitopes derived from Tg protein. We expect that this treatment will down-regulate B-cell autoreactivity by delivering a strong inhibitory signal. Three peptides-two epitope-predicted ones derived from Tg and another irrelevant peptide-were synthesized and then coupled with monoclonal anti-human CR1 antibody to construct three chimeric molecules. The binding to CD35 on human B cells and the effects of the chimeric constructs on PBMC and TMC from patients with HT were tested using flow cytometry, ELISpot assay, and immunoenzyme methods. We found that after the chemical conjugation, all chimeras retained their receptor-binding capacity, and the Tg epitopes could be recognized by anti-Tg autoantibodies in the patients' sera. This treatment downregulated B-cell autoreactivity and cell proliferation, inhibited Tg-specific B-cell differentiation to plasmablasts and promoted apoptosis to the targeted cells. The treatment of PBMCs from HT patients with Tg-epitope-carrying chimeric molecules affects the activity of Tg-specific autoreactive B lymphocytes, delivering to them a strong suppressive signal.
    Language English
    Publishing date 2022-12-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms232315083
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: A torpor-like state (TLS) in mice slows blood epigenetic aging and prolongs healthspan.

    Jayne, Lorna / Lavin-Peter, Aurora / Roessler, Julian / Tyshkovskiy, Alexander / Antoszewski, Mateusz / Ren, Erika / Markovski, Aleksandar / Sun, Senmiao / Yao, Hanqi / Sankaran, Vijay G / Gladyshev, Vadim N / Brooke, Robert T / Horvath, Steve / Griffith, Eric C / Hrvatin, Sinisa

    bioRxiv : the preprint server for biology

    2024  

    Abstract: Torpor and hibernation are extreme physiological adaptations of homeotherms associated with pro-longevity effects. Yet the underlying mechanisms of how torpor affects aging, and whether hypothermic and hypometabolic states can be induced to slow aging ... ...

    Abstract Torpor and hibernation are extreme physiological adaptations of homeotherms associated with pro-longevity effects. Yet the underlying mechanisms of how torpor affects aging, and whether hypothermic and hypometabolic states can be induced to slow aging and increase health span, remain unknown. We demonstrate that the activity of a spatially defined neuronal population in the avMLPA, which has previously been identified as a torpor-regulating brain region, is sufficient to induce a torpor like state (TLS) in mice. Prolonged induction of TLS slows epigenetic aging across multiple tissues and improves health span. We isolate the effects of decreased metabolic rate, long-term caloric restriction, and decreased core body temperature (T
    Language English
    Publishing date 2024-03-25
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.03.20.585828
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Investigation of the possibility for production of some stone fruit rootstocks by rooting cuttings

    Markovski Aleksandar / Popovska Melpomena / Gjamovski Viktor

    Acta Agriculturae Serbica, Vol 20, Iss 39, Pp 75-

    2015  Volume 83

    Abstract: This work evaluated the influence of different biostimulators (IBA and NAA) on rooting softwood and hardwood cuttings from some stone fruit rootstocks (Mirabolana, St. Julian A., St. Julian INRA, St. Julian Orleans, GF 8/1, GF 655/2, Alkavo, Gisela 4, ... ...

    Abstract This work evaluated the influence of different biostimulators (IBA and NAA) on rooting softwood and hardwood cuttings from some stone fruit rootstocks (Mirabolana, St. Julian A., St. Julian INRA, St. Julian Orleans, GF 8/1, GF 655/2, Alkavo, Gisela 4, Gisela 5, Weiroot 53 and Weiroot 158). The experiment was conducted in an experimental greenhouse at the Institute of Agriculture, Skopje. Rooting biostimulators were applied in 3 variants: a) IBA 2%, b) NAA 0.2%, c) IBA 2% without chemical protection. The GF/8 rootstock had the highest percent of rooted cuttings (50.8%), while some rootstocks (Weiroot 158 and Wieroot 53) produced negligible rooting of the cuttings (1.6%). Hardwood cuttings from the tested rootstocks had better rooting (20.3%) than softwood cuttings (6.0%). The first treatment (IBA 2%) was the most efficient for rooting cuttings (23.1%).
    Keywords softwood cutting ; hardwood cutting ; rooting ; Agriculture ; S
    Language English
    Publishing date 2015-01-01T00:00:00Z
    Publisher University of Kragujevac, Faculty of Agronomy, Cacak
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article: Application of High Performance Liquid Chromatography to the Analysis of Pesticide Residues in Apple Juice

    Velkoska-Markovska, Lenche / Petanovska-Ilievska, Biljana / Markovski, Aleksandar

    Contemporary Agriculture. 2018 Mar. 1, v. 67, no. 1

    2018  

    Abstract: The modern apple production involves the use of large amounts of pesticides that can be found in processed products such as apple juice. Harmful effects of pesticide residues on humans, especially children, are well known, hence the content of pesticide ... ...

    Abstract The modern apple production involves the use of large amounts of pesticides that can be found in processed products such as apple juice. Harmful effects of pesticide residues on humans, especially children, are well known, hence the content of pesticide residues in fruit, vegetables and their juices should be controlled. This study presents an application of a new, relatively simple and reliable analytical method for qualitative and quantitative determination of three organophosphorus and one organonitrogen pesticide residues in apple juices. The analysis utilizes reversed-phase high-performance liquid chromatography (RP-HPLC) followed by UV diode array detection. Prior to HPLC analysis, a solid-phase extraction (SPE) was used for analytes concentration and sample clean-up. Specificity, selectivity, linearity, precision, accuracy and limit of quantification (LOQ) were examined to assess the validity of the developed method. The method had satisfactory values of multiple correlation coefficients for calibration curves (R2 ≥ 0.95). The precision was evaluated for the retention times and peak areas, and the estimated values for relative standard deviations (RSD) were 0.05 % - 0.18 % and 0.09 % - 0.62 %, respectively, which indicated an excellent precision of the proposed method. Under the established conditions, the recovery of analytes was 93.80 % - 119.41 %, with relative standard deviations below 0.56 %. This method was successfully applied for determination of some organophosphorus and organonitrogen pesticide residues in apple juices which were taken from Macedonian markets. The achieved values for LOQs were low enough compared to the MRLs of the investigated pesticides in apple according to the Regulation (EC) No 396/2005. Detectable residues of the examined pesticides were not found in the analyzed samples.
    Keywords apple juice ; apples ; chemical species ; children ; crop production ; diodes ; fruits ; humans ; markets ; maximum residue limits ; pesticide residues ; pesticides ; quantitative analysis ; reversed-phase high performance liquid chromatography ; solid phase extraction ; standard deviation ; vegetables
    Language English
    Dates of publication 2018-0301
    Size p. 93-102.
    Publishing place De Gruyter Open
    Document type Article
    ZDB-ID 2676736-3
    ISSN 2466-4774 ; 0350-1205
    ISSN 2466-4774 ; 0350-1205
    DOI 10.2478/contagri-2018-0014
    Database NAL-Catalogue (AGRICOLA)

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