LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 9 of total 9

Search options

  1. Book ; Thesis: Entschlüsselung der dynamischen Evolution oligoklonaler Mutationshierarchien von Patienten mit myelodysplastischem Syndrom unter Therapie und bei Krankheitsprogression

    Mossner, Maximilian

    2016  

    Author's details vorgelegt von Maximilian Markus Wolf Moses Mossner
    Language German
    Size 115 Blätter, Illustrationen, Diagramme, 30 cm
    Publishing place Heidelberg
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Dissertation, Ruprecht-Karls-Universität Heidelberg, 2016
    HBZ-ID HT019310621
    Database Catalogue ZB MED Medicine, Health

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2017 E 750 order for loan Magazin

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: High-Throughput Screening for CEBPD-Modulating Compounds in THP-1-Derived Reporter Macrophages Identifies Anti-Inflammatory HDAC and BET Inhibitors

    Tatjana Ullmann / Sonja Luckhardt / Markus Wolf / Michael J. Parnham / Eduard Resch

    International Journal of Molecular Sciences, Vol 22, Iss 3022, p

    2021  Volume 3022

    Abstract: This study aimed to identify alternative anti-inflammatory compounds that modulate the activity of a relevant transcription factor, CCAAT/enhancer binding protein delta (C/EBPδ). C/EBPδ is a master regulator of inflammatory responses in macrophages (Mϕ) ... ...

    Abstract This study aimed to identify alternative anti-inflammatory compounds that modulate the activity of a relevant transcription factor, CCAAT/enhancer binding protein delta (C/EBPδ). C/EBPδ is a master regulator of inflammatory responses in macrophages (Mϕ) and is mainly regulated at the level of CEBPD gene transcription initiation. To screen for CEBPD -modulating compounds, we generated a THP-1-derived reporter cell line stably expressing secreted alkaline phosphatase (SEAP) under control of the defined CEBPD promoter ( CEBPD::SEAP ). A high-throughput screening of LOPAC ®1280 and ENZO ®774 libraries on LPS- and IFN-γ-activated THP-1 reporter Mϕ identified four epigenetically active hits: two bromodomain and extraterminal domain (BET) inhibitors, I-BET151 and Ro 11-1464, as well as two histone deacetylase (HDAC) inhibitors, SAHA and TSA. All four hits markedly and reproducibly upregulated SEAP secretion and CEBPD::SEAP mRNA expression, confirming screening assay reliability. Whereas BET inhibitors also upregulated the mRNA expression of the endogenous CEBPD , HDAC inhibitors completely abolished it. All hits displayed anti-inflammatory activity through the suppression of IL-6 and CCL2 gene expression. However, I-BET151 and HDAC inhibitors simultaneously upregulated the mRNA expression of pro-inflammatory IL-1ß . The modulation of CEBPD gene expression shown in this study contributes to our understanding of inflammatory responses in Mϕ and may offer an approach to therapy for inflammation-driven disorders.
    Keywords CEBPD ; phenotypic screening ; anti-inflammatory drug ; SEAP ; I-BET151 ; Ro 11-1464 ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 570
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Challenges and best practices for digital unstructured data enrichment in health research

    Jana Sedlakova / Paola Daniore / Andrea Horn Wintsch / Markus Wolf / Mina Stanikic / Christina Haag / Chloé Sieber / Gerold Schneider / Kaspar Staub / Dominik Alois Ettlin / Oliver Grübner / Fabio Rinaldi / Viktor von Wyl

    PLOS Digital Health, Vol 2, Iss 10, p e

    A systematic narrative review.

    2023  Volume 0000347

    Abstract: Digital data play an increasingly important role in advancing health research and care. However, most digital data in healthcare are in an unstructured and often not readily accessible format for research. Unstructured data are often found in a format ... ...

    Abstract Digital data play an increasingly important role in advancing health research and care. However, most digital data in healthcare are in an unstructured and often not readily accessible format for research. Unstructured data are often found in a format that lacks standardization and needs significant preprocessing and feature extraction efforts. This poses challenges when combining such data with other data sources to enhance the existing knowledge base, which we refer to as digital unstructured data enrichment. Overcoming these methodological challenges requires significant resources and may limit the ability to fully leverage their potential for advancing health research and, ultimately, prevention, and patient care delivery. While prevalent challenges associated with unstructured data use in health research are widely reported across literature, a comprehensive interdisciplinary summary of such challenges and possible solutions to facilitate their use in combination with structured data sources is missing. In this study, we report findings from a systematic narrative review on the seven most prevalent challenge areas connected with the digital unstructured data enrichment in the fields of cardiology, neurology and mental health, along with possible solutions to address these challenges. Based on these findings, we developed a checklist that follows the standard data flow in health research studies. This checklist aims to provide initial systematic guidance to inform early planning and feasibility assessments for health research studies aiming combining unstructured data with existing data sources. Overall, the generality of reported unstructured data enrichment methods in the studies included in this review call for more systematic reporting of such methods to achieve greater reproducibility in future studies.
    Keywords Computer applications to medicine. Medical informatics ; R858-859.7
    Subject code 306
    Language English
    Publishing date 2023-10-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  4. Article ; Online: Stereotyping in the digital age

    Tabea Meier / Ryan L Boyd / Matthias R Mehl / Anne Milek / James W Pennebaker / Mike Martin / Markus Wolf / Andrea B Horn

    PLoS ONE, Vol 15, Iss 12, p e

    Male language is "ingenious", female language is "beautiful" - and popular.

    2020  Volume 0243637

    Abstract: The huge power for social influence of digital media may come with the risk of intensifying common societal biases, such as gender and age stereotypes. Speaker's gender and age also behaviorally manifest in language use, and language may be a powerful ... ...

    Abstract The huge power for social influence of digital media may come with the risk of intensifying common societal biases, such as gender and age stereotypes. Speaker's gender and age also behaviorally manifest in language use, and language may be a powerful tool to shape impact. The present study took the example of TED, a highly successful knowledge dissemination platform, to study online influence. Our goal was to investigate how gender- and age-linked language styles-beyond chronological age and identified gender-link to talk impact and whether this reflects gender and age stereotypes. In a pre-registered study, we collected transcripts of TED Talks along with their impact measures, i.e., views and ratios of positive and negative talk ratings, from the TED website. We scored TED Speakers' (N = 1,095) language with gender- and age-morphed language metrics to obtain measures of female versus male, and younger versus more senior language styles. Contrary to our expectations and to the literature on gender stereotypes, more female language was linked to higher impact in terms of quantity, i.e., more talk views, and this was particularly the case among talks with a lot of views. Regarding quality of impact, language signatures of gender and age predicted different types of positive and negative ratings above and beyond main effects of speaker's gender and age. The differences in ratings seem to reflect common stereotype contents of warmth (e.g., "beautiful" for female, "courageous" for female and senior language) versus competence (e.g., "ingenious", "informative" for male language). The results shed light on how verbal behavior may contribute to stereotypical evaluations. They also illuminate how, within new digital social contexts, female language might be uniquely rewarded and, thereby, an underappreciated but highly effective tool for social influence. WC = 286 (max. 300 words).
    Keywords Medicine ; R ; Science ; Q
    Subject code 401
    Language English
    Publishing date 2020-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article ; Online: A SARS-CoV-2 cytopathicity dataset generated by high-content screening of a large drug repurposing collection

    Bernhard Ellinger / Denisa Bojkova / Andrea Zaliani / Jindrich Cinatl / Carsten Claussen / Sandra Westhaus / Oliver Keminer / Jeanette Reinshagen / Maria Kuzikov / Markus Wolf / Gerd Geisslinger / Philip Gribbon / Sandra Ciesek

    Scientific Data, Vol 8, Iss 1, Pp 1-

    2021  Volume 10

    Abstract: Measurement(s) cytotoxicity • killing by virus of host cell Technology Type(s) bright-field microscopy Factor Type(s) concentration • incubation time Sample Characteristic - Organism Homo sapiens Machine-accessible metadata file describing the reported ... ...

    Abstract Measurement(s) cytotoxicity • killing by virus of host cell Technology Type(s) bright-field microscopy Factor Type(s) concentration • incubation time Sample Characteristic - Organism Homo sapiens Machine-accessible metadata file describing the reported data: https://doi.org/10.6084/m9.figshare.13562570
    Keywords Science ; Q
    Language English
    Publishing date 2021-02-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article ; Online: Antiviral activity of natural phenolic compounds in complex at an allosteric site of SARS-CoV-2 papain-like protease

    Vasundara Srinivasan / Hévila Brognaro / Prince R. Prabhu / Edmarcia Elisa de Souza / Sebastian Günther / Patrick Y. A. Reinke / Thomas J. Lane / Helen Ginn / Huijong Han / Wiebke Ewert / Janina Sprenger / Faisal H. M. Koua / Sven Falke / Nadine Werner / Hina Andaleeb / Najeeb Ullah / Bruno Alves Franca / Mengying Wang / Angélica Luana C. Barra /
    Markus Perbandt / Martin Schwinzer / Christina Schmidt / Lea Brings / Kristina Lorenzen / Robin Schubert / Rafael Rahal Guaragna Machado / Erika Donizette Candido / Danielle Bruna Leal Oliveira / Edison Luiz Durigon / Stephan Niebling / Angelica Struve Garcia / Oleksandr Yefanov / Julia Lieske / Luca Gelisio / Martin Domaracky / Philipp Middendorf / Michael Groessler / Fabian Trost / Marina Galchenkova / Aida Rahmani Mashhour / Sofiane Saouane / Johanna Hakanpää / Markus Wolf / Maria Garcia Alai / Dusan Turk / Arwen R. Pearson / Henry N. Chapman / Winfried Hinrichs / Carsten Wrenger / Alke Meents

    Communications Biology, Vol 5, Iss 1, Pp 1-

    2022  Volume 12

    Abstract: Three natural phenolic compounds are found to bind to an allosteric site in SARS-CoV-2 papain-like protease and exhibit antiviral activity in vitro, showing potential as starting scaffolds for drug design. ...

    Abstract Three natural phenolic compounds are found to bind to an allosteric site in SARS-CoV-2 papain-like protease and exhibit antiviral activity in vitro, showing potential as starting scaffolds for drug design.
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2022-08-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Article: VCP/p97 Extracts Sterically Trapped Ku70/80 Rings from DNA in Double-Strand Break Repair

    van den Boom, Johannes / Anne Riemer / Christian Zierhut / Fanghua Li / Farnusch Kaschani / George Iliakis / Hemmo Meyer / Hironori Funabiki / Lena Weimann / Markus Kaiser / Markus Wolf / Nina Schulze

    Molecular cell. 2016 Oct. 06, v. 64, no. 1

    2016  

    Abstract: During DNA double-strand break (DSB) repair, the ring-shaped Ku70/80 complex becomes trapped on DNA and needs to be actively extracted, but it has remained unclear what provides the required energy. By means of reconstitution of DSB repair on beads, we ... ...

    Abstract During DNA double-strand break (DSB) repair, the ring-shaped Ku70/80 complex becomes trapped on DNA and needs to be actively extracted, but it has remained unclear what provides the required energy. By means of reconstitution of DSB repair on beads, we demonstrate here that DNA-locked Ku rings are released by the AAA-ATPase p97. To achieve this, p97 requires ATP hydrolysis, cooperates with the Ufd1-Npl4 ubiquitin-adaptor complex, and specifically targets Ku80 that is modified by K48-linked ubiquitin chains. In U2OS cells, chemical inhibition of p97 or siRNA-mediated depletion of p97 or its adapters impairs Ku80 removal after non-homologous end joining of DSBs. Moreover, this inhibition attenuates early steps in homologous recombination, consistent with p97-driven Ku release also affecting repair pathway choice. Thus, our data answer a central question regarding regulation of Ku in DSB repair and illustrate the ability of p97 to segregate even tightly bound protein complexes for release from DNA.
    Keywords adenosine triphosphate ; DNA ; DNA repair ; energy ; homologous recombination ; hydrolysis ; ubiquitin
    Language English
    Dates of publication 2016-1006
    Size p. 189-198.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 1415236-8
    ISSN 1097-4164 ; 1097-2765
    ISSN (online) 1097-4164
    ISSN 1097-2765
    DOI 10.1016/j.molcel.2016.08.037
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 2005/501: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  8. Article ; Online: Chemical Analysis and Ecotoxicological Effects of UV Absorbing Organic Chemicals in Aquatic Environments

    Karl Fent / Petra Kunz / Markus Wolf / Hansruedi Schmutz / Daniel Gygax / Clemens Schanné

    CHIMIA, Vol 58, Iss

    2004  Volume 5

    Abstract: Substances that exert adverse effects on reproduction and fertility are a cause of concern. UV filters used in sunscreens and in the protection of materials may enter aquatic environments. Properties of UV filters such as environmental persistency and ... ...

    Abstract Substances that exert adverse effects on reproduction and fertility are a cause of concern. UV filters used in sunscreens and in the protection of materials may enter aquatic environments. Properties of UV filters such as environmental persistency and high lipophilicity make an environmental risk assessment desirable, in particular as some of them show hormonal activity at high concentrations. In this research project conducted within the National Research Programme NRP50, hormonal activities of UV filters are evaluated in vitro in recombinant yeast system and in vivo in fish and frogs.
    Keywords Ecotoxicology ; Endocrine disrupters ; Estrogenic activity ; Uv filters ; Chemistry ; QD1-999
    Language German
    Publishing date 2004-05-01T00:00:00Z
    Publisher Swiss Chemical Society
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  9. Article ; Online: Exploiting the 2‑Amino-1,3,4-thiadiazole Scaffold To Inhibit Trypanosoma brucei Pteridine Reductase in Support of Early-Stage Drug Discovery

    Pasquale Linciano / Alice Dawson / Ina Pöhner / David M. Costa / Monica S. Sá / Anabela Cordeiro-da-Silva / Rosaria Luciani / Sheraz Gul / Gesa Witt / Bernhard Ellinger / Maria Kuzikov / Philip Gribbon / Jeanette Reinshagen / Markus Wolf / Birte Behrens / Véronique Hannaert / Paul A. M. Michels / Erika Nerini / Cecilia Pozzi /
    Flavio di Pisa / Giacomo Landi / Nuno Santarem / Stefania Ferrari / Puneet Saxena / Sandra Lazzari / Giuseppe Cannazza / Lucio H. Freitas-Junior / Carolina B. Moraes / Bruno S. Pascoalino / Laura M. Alcântara / Claudia P. Bertolacini / Vanessa Fontana / Ulrike Wittig / Wolfgang Müller / Rebecca C. Wade / William N. Hunter / Stefano Mangani / Luca Costantino / Maria P. Costi

    ACS Omega, Vol 2, Iss 9, Pp 5666-

    2017  Volume 5683

    Keywords Chemistry ; QD1-999
    Language English
    Publishing date 2017-09-01T00:00:00Z
    Publisher American Chemical Society
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top