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Article: Revealing the Prevalence of

Castillo-Castillo, José María / Rufino-Moya, Pablo José / Martínez-Moreno, Álvaro / Salvador Castaño, Ángela / Martínez-Moreno, Francisco Javier / Leva, Rafael Zafra

Pathogens (Basel, Switzerland)

2024 Volume 13, Issue 4

Abstract: This research work focused on the prevalence ... ...

Abstract	This research work focused on the prevalence of
Language	English
Publishing date	2024-03-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens13040281
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Article ; Online: Antiparasitic Tannin-Rich Plants from the South of Europe for Grazing Livestock: A Review

Rodríguez-Hernández, Pablo / Reyes-Palomo, Carolina / Sanz-Fernández, Santos / Rufino-Moya, Pablo José / Zafra, Rafael / Martínez-Moreno, Francisco Javier / Rodríguez-Estévez, Vicente / Díaz-Gaona, Cipriano

Animals. 2023 Jan. 05, v. 13, no. 2

2023

Abstract: Internal parasites are one of the main causes of health threats in livestock production, especially in extensive livestock farming. Despite the environmental toxic effects (loss of dung beetles, biodiversity, and other issues) and resistance phenomenon ... ...

Abstract	Internal parasites are one of the main causes of health threats in livestock production, especially in extensive livestock farming. Despite the environmental toxic effects (loss of dung beetles, biodiversity, and other issues) and resistance phenomenon derived from their prolonged use, anti-parasitic chemical pharmaceuticals are frequently used, even in organic farming. Such a situation within the context of climate change requires urgent exploration of alternative compounds to solve these problems and apparent conflicts between organic farming objectives regarding the environment, public health, and animal health. This review is focused on some plants (Artemisia spp., Cichorium intybus L., Ericaceae family, Hedysarum coronarium L., Lotus spp., Onobrychis viciifolia Scop.) that are well known for their antiparasitic effect, are voluntarily grazed and ingested, and can be spontaneously found or cultivated in southern Europe and other regions with a Mediterranean climate. The differences found between effectiveness, parasite species affected, in vitro/in vivo experiments, and active compounds are explored. A total of 87 papers where antiparasitic activity of those plants have been studied are included in this review; 75% studied the effect on ruminant parasites, where gastrointestinal nematodes were the parasite group most studied (70%), and these included natural (31%) and experimental (37%) infections.
Keywords	Artemisia ; Cichorium intybus ; Ericaceae ; Hedysarum coronarium ; Lotus ; Mediterranean climate ; Onobrychis viciifolia ; antiparasitic agents ; antiparasitic properties ; biodiversity ; climate change ; feces ; gastrointestinal nematodes ; livestock ; livestock production ; public health ; ruminants ; toxicity ; Europe ; Southern European region
Language	English
Dates of publication	2023-0105
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2606558-7
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani13020201
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Evaluation of Th1/Th2, regulatory cytokines and transcriptional factor FoxP3 in sheep immunized with a partially protective and non-protective vaccine and challenged with Fasciola hepatica.

Ruiz-Campillo, María Teresa / Pacheco, Isabel Lourdes / Abril, Nieves / Bautista, María José / Martínez-Moreno, Álvaro / Martínez-Moreno, Francisco Javier / Buffoni, Leandro / Pérez, José / Molina-Hernández, Verónica / Zafra, Rafael

Veterinary research

2024 Volume 55, Issue 1, Page(s) 53

Abstract: Gene expression for Th1/Th2 cytokines (IL-4 and IFN-ɣ), regulatory cytokines (TGF-β and IL-10) and the transcriptional factor FoxP3 was analyzed in the liver and hepatic lymph nodes (HLN) from sheep immunized with partially protective and non-protective ... ...

Abstract	Gene expression for Th1/Th2 cytokines (IL-4 and IFN-ɣ), regulatory cytokines (TGF-β and IL-10) and the transcriptional factor FoxP3 was analyzed in the liver and hepatic lymph nodes (HLN) from sheep immunized with partially protective and non-protective vaccine candidates and challenged with Fasciola hepatica. FoxP3 T cells were also evaluated by immunohistochemistry (IHQ). The most remarkable difference between the partially protected vaccinated (V1) group and the non-protected vaccinated (V2) group was a more severe expansion of FoxP3 T cells recorded by IHQ in both the liver and HLN of the V2 group as compared to the V1 group, whereas no differences were found between the V2 group and the infected control (IC) group. Similar results were recorded for FoxP3 gene expression although significant differences among V1 and V2 groups were only significant in the HLN, while FoxP3 gene expression was very similar in the V2 and IC groups both in the liver and HLN. No significant differences for the remaining cytokines were recorded between the V1 and V2 groups, but in the liver the V2 group shows significant increases of IFN-ɣ and IL-10 as compared to the uninfected control (UC) group whereas the V1 group did not. The lower expansion of FoxP3 T cells and lower increase of IFN-ɣ and IL-10 in the partially protected vaccinated group may be related with lower hepatic lesions and fluke burdens recorded in this group as compared to the other two infected groups. The most relevant change in regulatory cytokine gene expression was the significant increase of TGF-β in the liver of IC, V1 and V2 groups as compared to the UC group, which could be related to hepatic lesions.
MeSH term(s)	Animals ; Fascioliasis/veterinary ; Fascioliasis/prevention & control ; Fascioliasis/immunology ; Fasciola hepatica/immunology ; Sheep ; Forkhead Transcription Factors/metabolism ; Sheep Diseases/prevention & control ; Sheep Diseases/immunology ; Sheep Diseases/parasitology ; Cytokines/metabolism ; Liver/parasitology ; Liver/immunology ; Vaccines/immunology ; Vaccines/administration & dosage ; Th1 Cells/immunology ; Lymph Nodes/immunology ; Female ; Th2 Cells/immunology
Chemical Substances	Forkhead Transcription Factors ; Cytokines ; Vaccines
Language	English
Publishing date	2024-04-24
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1146298-x
ISSN	1297-9716 ; 0928-4249
ISSN (online)	1297-9716
ISSN	0928-4249
DOI	10.1186/s13567-024-01308-8
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Article: Antiparasitic Tannin-Rich Plants from the South of Europe for Grazing Livestock: A Review.

Animals : an open access journal from MDPI

2023 Volume 13, Issue 2

Abstract	Internal parasites are one of the main causes of health threats in livestock production, especially in extensive livestock farming. Despite the environmental toxic effects (loss of dung beetles, biodiversity, and other issues) and resistance phenomenon derived from their prolonged use, anti-parasitic chemical pharmaceuticals are frequently used, even in organic farming. Such a situation within the context of climate change requires urgent exploration of alternative compounds to solve these problems and apparent conflicts between organic farming objectives regarding the environment, public health, and animal health. This review is focused on some plants (
Language	English
Publishing date	2023-01-05
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2606558-7
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani13020201
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Article: Fasciolosis: pathogenesis, host-parasite interactions, and implication in vaccine development.

Flores-Velázquez, Luis Miguel / Ruiz-Campillo, María Teresa / Herrera-Torres, Guillem / Martínez-Moreno, Álvaro / Martínez-Moreno, Francisco Javier / Zafra, Rafael / Buffoni, Leandro / Rufino-Moya, Pablo José / Molina-Hernández, Verónica / Pérez, José

Frontiers in veterinary science

2023 Volume 10, Page(s) 1270064

Abstract: ... Fasciola ... ...

Abstract	Fasciola hepatica
Language	English
Publishing date	2023-12-11
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2834243-4
ISSN	2297-1769
ISSN	2297-1769
DOI	10.3389/fvets.2023.1270064
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Article ; Online: Serosurveillance of Trichinella sp. in wild boar and Iberian domestic suids in Mediterranean ecosystems of southwestern Spain.

Buffoni, Leandro / Cano-Terriza, David / Jiménez-Martín, Débora / Jiménez-Ruiz, Saúl / Martínez-Moreno, Álvaro / Martínez-Moreno, Francisco Javier / Zafra, Rafael / Pérez-Caballero, Raúl / Risalde, María Ángeles / Gómez-Guillamón, Félix / García-Bocanegra, Ignacio

Zoonoses and public health

2023 Volume 71, Issue 2, Page(s) 191–199

Abstract: Aims: A cross-sectional study was carried out to assess the seroprevalence and risk factors associated with Trichinella spp. exposure in wild boar and Iberian domestic pigs from Mediterranean ecosystems of southwestern Spain.: Methods and results: ... ...

Abstract	Aims: A cross-sectional study was carried out to assess the seroprevalence and risk factors associated with Trichinella spp. exposure in wild boar and Iberian domestic pigs from Mediterranean ecosystems of southwestern Spain. Methods and results: Serum samples from 1360 wild boar and 439 Iberian domestic pigs were obtained during 2015-2020, from regions where Iberian pigs are raised under extensive conditions, hence sharing habitat with wild boar. Seropositivity was found in 7.4% (100/1360; 95% CI: 6.1-8.9) of the wild boar analysed. In this species, the individual seroprevalence ranged from 3.6% (8/223) (hunting season 2016-2017) to 11.4% (37/326) (2018-2019). A significant higher seropositivity was observed during the hunting season 2018-2019 (p < 0.009: OR = 3.07; 95% CI = 1.32-7.18) and one statistically significant cluster was detected within the studied area, in south central Andalusia [Relative Risk (RR) = 2.9; p = 0.037]. Females showed a significantly higher seroprevalence than males (8.7% vs. 5.8%) (p < 0.001: OR = 1.58; 95% CI = 1.08-2.32). No seropositivity to Trichinella spp. was detected in Iberian domestic pigs (0.0%; 95% CI: 0.0-0.9). Conclusions: Although wild boar play an important role as a reservoir of Trichinella sp. in the Mediterranean ecosystems of southwestern Spain, our results suggest that the wild boar production system does not seem to pose a risk of Trichinella exposure to domestic pigs, despite sharing habitats in these ecosystems.
MeSH term(s)	Male ; Female ; Swine ; Animals ; Spain/epidemiology ; Ecosystem ; Seroepidemiologic Studies ; Cross-Sectional Studies ; Sus scrofa ; Trichinella ; Swine Diseases/epidemiology ; Trichinellosis/epidemiology ; Trichinellosis/veterinary
Language	English
Publishing date	2023-11-27
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2271118-1
ISSN	1863-2378 ; 1863-1959
ISSN (online)	1863-2378
ISSN	1863-1959
DOI	10.1111/zph.13098
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Article: Characterization of dendritic cells and follicular dendritic cells in the hepatic lymph nodes and liver of sheep experimentally infected with Fasciola hepatica

Ruiz-Campillo, María Teresa / Molina-Hernández, Verónica / Bautista, María José / Pacheco, Isabel L / Zafra, Rafael / Buffoni, Leandro / Martínez-Moreno, Francisco Javier / Martínez-Moreno, Alvaro / Pérez, José

Veterinary research. 2020 Dec., v. 51, no. 1

2020

Abstract: Fasciola hepatica has been shown to have a high capacity for immunomodulation of the host response, making the development of protective vaccines extremely difficult. One of these immunomodulation mechanisms is the impairment of dendritic cells (DC) ... ...

Abstract	Fasciola hepatica has been shown to have a high capacity for immunomodulation of the host response, making the development of protective vaccines extremely difficult. One of these immunomodulation mechanisms is the impairment of dendritic cells (DC) maturation and, therefore, suppression of antigenic presentation. The aim of this study was to evaluate the pathological changes as well as the characterization of two antigen presenting cells, DC (CD1b, CD83 and MHC-II positive) and follicular dendritic cells (FDC) (CNA.42, S100 and CD83 positive) by immunohistochemistry in the hepatic lymph nodes (HLN) and livers of sheep during the early stages of infection with F. hepatica [9 and 18 days post-infection (dpi)], compared with an uninfected group (UC) as a control. The results revealed a marked hyperplasia of HLN germinal centres at 9 and, in particular, 18 dpi, with respect to the UC group, with coincidental increased expression of CNA.42 in FDC of lymphoid follicles and CD1b in the DC of paracortical areas at 18 dpi. However, the expression of MHC-II and CD83 decreased at 9 and, particularly, at 18 dpi in HLN compared with that in the UC group. Since both markers are related to active presentation of antigens by DC and FDC, the results of the present study suggest that, despite the marked hyperplasia of HLN and increase in DC and FDC numbers during early stages of infection, the DC and FDC antigenic presentation capacity, as suggested by the expression of the markers MHC-II and CD83, is suppressed by the parasite. This suppression was not observed in the liver, probably because of the low number of DC. This is the first study of the immunophenotype of DCs and FDC in sheep infected with F. hepatica.
Keywords	Fasciola hepatica ; antigens ; dendritic cells ; hyperplasia ; immunohistochemistry ; immunomodulation ; immunophenotype ; liver ; lymph nodes ; major histocompatibility complex ; parasites ; sheep ; vaccines
Language	English
Dates of publication	2020-12
Size	p. 33.
Publishing place	BioMed Central
Document type	Article
ZDB-ID	1146298-x
ISSN	1297-9716 ; 0928-4249
ISSN (online)	1297-9716
ISSN	0928-4249
DOI	10.1186/s13567-020-00757-1
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Characterization of dendritic cells and follicular dendritic cells in the hepatic lymph nodes and liver of sheep experimentally infected with Fasciola hepatica.

Veterinary research

2020 Volume 51, Issue 1, Page(s) 33

Abstract	Fasciola hepatica has been shown to have a high capacity for immunomodulation of the host response, making the development of protective vaccines extremely difficult. One of these immunomodulation mechanisms is the impairment of dendritic cells (DC) maturation and, therefore, suppression of antigenic presentation. The aim of this study was to evaluate the pathological changes as well as the characterization of two antigen presenting cells, DC (CD1b, CD83 and MHC-II positive) and follicular dendritic cells (FDC) (CNA.42, S100 and CD83 positive) by immunohistochemistry in the hepatic lymph nodes (HLN) and livers of sheep during the early stages of infection with F. hepatica [9 and 18 days post-infection (dpi)], compared with an uninfected group (UC) as a control. The results revealed a marked hyperplasia of HLN germinal centres at 9 and, in particular, 18 dpi, with respect to the UC group, with coincidental increased expression of CNA.42 in FDC of lymphoid follicles and CD1b in the DC of paracortical areas at 18 dpi. However, the expression of MHC-II and CD83 decreased at 9 and, particularly, at 18 dpi in HLN compared with that in the UC group. Since both markers are related to active presentation of antigens by DC and FDC, the results of the present study suggest that, despite the marked hyperplasia of HLN and increase in DC and FDC numbers during early stages of infection, the DC and FDC antigenic presentation capacity, as suggested by the expression of the markers MHC-II and CD83, is suppressed by the parasite. This suppression was not observed in the liver, probably because of the low number of DC. This is the first study of the immunophenotype of DCs and FDC in sheep infected with F. hepatica.
MeSH term(s)	Animals ; Dendritic Cells/pathology ; Dendritic Cells, Follicular/pathology ; Fasciola hepatica/physiology ; Fascioliasis/pathology ; Fascioliasis/veterinary ; Genetic Markers ; Liver/pathology ; Lymph Nodes/pathology ; Sheep ; Sheep Diseases/pathology
Chemical Substances	Genetic Markers
Language	English
Publishing date	2020-03-04
Publishing country	England
Document type	Journal Article
ZDB-ID	1146298-x
ISSN	1297-9716 ; 0928-4249
ISSN (online)	1297-9716
ISSN	0928-4249
DOI	10.1186/s13567-020-00757-1
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Article: Humoral response (IgG) of goats experimentally infected with Fasciola hepatica against cysteine proteinases of adult fluke.

Ruiz, Antonio / Molina, José Manuel / González, Jorge / Martínez-Moreno, Francisco Javier / Gutiérrez, Pedro Nolasco / Martínez-Moreno, Alvaro

Veterinary research

2003 Volume 34, Issue 4, Page(s) 435–443

Abstract: The use of cysteine proteinases from Fasciola hepatica adult flukes for the serodiagnosis of caprine fasciolosis by means of an indirect ELISA test was studied. Two proteolytic fractions from adult fluke homogenates, with apparent molecular weights of 28 ...

Abstract	The use of cysteine proteinases from Fasciola hepatica adult flukes for the serodiagnosis of caprine fasciolosis by means of an indirect ELISA test was studied. Two proteolytic fractions from adult fluke homogenates, with apparent molecular weights of 28 and 34 kDa (P28 and P34 respectively), were characterised as cysteine proteinases using azocasein assays and gelatin gel analysis. Both P28 and P34 fractions were electroluted and used as antigens in two different indirect ELISA tests. Serum IgG levels against P28 and P34 in goats given an experimental primary infection with 200 metacercariae or in goats given two experimental infections with 200 metacercariae were determined and compared with those observed in an uninfected control group. ELISA tests using both cysteine proteases showed a rapid and consistent detection of specific IgG in all experimentally infected goats. The IgG response to P28 was the first to be detected as early as 2-3 weeks post-infection and remained elevated throughout the experiment. The response to P34 was detected later (4-6 wpi) and disappeared in some animals at 18 wpi, while flukes were still present in the bile ducts. No significant differences were observed between the anti-P28 and anti-P34 IgG responses between animals receiving a primary or a challenge infection. The results of our study, although preliminary, are promising since the P28 ELISA described here may be a reliable method for the immunodiagnosis of F. hepatica infection in goats.
MeSH term(s)	Animals ; Antibodies, Helminth/biosynthesis ; Antibodies, Helminth/blood ; Cross Reactions ; Cysteine Endopeptidases/immunology ; Enzyme-Linked Immunosorbent Assay/methods ; Enzyme-Linked Immunosorbent Assay/veterinary ; Fasciola hepatica/enzymology ; Fasciola hepatica/immunology ; Fascioliasis/diagnosis ; Fascioliasis/immunology ; Fascioliasis/veterinary ; Goat Diseases/diagnosis ; Goat Diseases/immunology ; Goats ; Immunoglobulin G/biosynthesis ; Immunoglobulin G/blood ; Male ; Random Allocation
Chemical Substances	Antibodies, Helminth ; Immunoglobulin G ; Cysteine Endopeptidases (EC 3.4.22.-)
Language	English
Publishing date	2003-07
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1146298-x
ISSN	1297-9716 ; 0928-4249
ISSN (online)	1297-9716
ISSN	0928-4249
DOI	10.1051/vetres:2003016
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