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  1. Article ; Online: Senescence on Dental Pulp Cells: Effects on Morphology, Migration, Proliferation, and Immune Response.

    de Farias, Jade Ormondes / da Costa Sousa, Maurício Gonçalves / Martins, Danilo César Mota / de Oliveira, Mayara Alves / Takahashi, Isadora / de Sousa, Larissa Barbosa / da Silva, Ingrid Gracielle Martins / Corrêa, José Raimundo / Silva Carvalho, Amandda Évelin / Saldanha-Araújo, Felipe / Rezende, Taia Maria Berto

    Journal of endodontics

    2024  Volume 50, Issue 3, Page(s) 362–369

    Abstract: Introduction: Evidence indicates that senescence can affect essential dental pulp functions, such as defense capacity and repair, consequently affecting the successes of conservative endodontic treatments. This study aims to evaluate the effects of ... ...

    Abstract Introduction: Evidence indicates that senescence can affect essential dental pulp functions, such as defense capacity and repair, consequently affecting the successes of conservative endodontic treatments. This study aims to evaluate the effects of senescence on the morphology, migration, proliferation, and immune response of human dental pulp cells.
    Methods: Cells were treated with doxorubicin to induce senescence, confirmed by β-galactosidase staining. Morphological changes, cellular proliferation, and migration were evaluated by scanning electron microscopy, trypan blue cells, and the scratch method, respectively. Modifications in the immune response were evaluated by measuring the genes for pro-inflammatory cytokines tumor necrosis factor alpha and interleukin (IL)-6 and anti-inflammatory cytokines transforming growth factor beta 1 and IL-10 using the real time polymerase chain reaction assay.
    Results: An increase in cell size and a decrease in the number of extensions were observed in senescent cells. A reduction in the proliferative and migratory capacity was also found in senescent cells. In addition, there was an increase in the gene expression of inflammatory cytokines tumor necrosis factor alpha and IL-6 and a decrease in the gene expression of IL-10 and transforming growth factor beta-1, suggesting an exacerbated inflammatory situation associated with immunosuppression.
    Conclusions: Cellular senescence is possibly a condition that affects prognoses of conservative endodontic treatments, as it affects primordial cellular functions related to this treatment.
    MeSH term(s) Humans ; Dental Pulp/metabolism ; Cell Differentiation ; Interleukin-10/metabolism ; Tumor Necrosis Factor-alpha/metabolism ; Cytokines/metabolism ; Cell Proliferation ; Interleukin-6/metabolism ; Immunity ; Cellular Senescence ; Cells, Cultured
    Chemical Substances Interleukin-10 (130068-27-8) ; Tumor Necrosis Factor-alpha ; Cytokines ; Interleukin-6
    Language English
    Publishing date 2024-01-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 752412-2
    ISSN 1878-3554 ; 0099-2399
    ISSN (online) 1878-3554
    ISSN 0099-2399
    DOI 10.1016/j.joen.2023.12.009
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Host defense peptides combined with MTA extract increase the repair in dental pulp cells: in vitro and ex vivo study.

    Silva, Poliana Amanda Oliveira / Martins, Danilo César Mota / de Castro Cantuária, Ana Paula / de Andrade, Rosangela V / Lacorte, Cristiano / de Almeida, Jeeser Alves / Aguiar, Lana Ribeiro / Corrêa, José Raimundo / da Silva, Ingrid Gracielle Martins / Franco, Octávio Luiz / Rezende, Taia Maria Berto

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 9531

    Abstract: Host Defense Peptides (HDPs) have, in previous studies, been demonstrating antimicrobial, anti-inflammatory, and immunomodulatory capacity, important factors in the repair process. Knowing these characteristics, this article aims to evaluate the ... ...

    Abstract Host Defense Peptides (HDPs) have, in previous studies, been demonstrating antimicrobial, anti-inflammatory, and immunomodulatory capacity, important factors in the repair process. Knowing these characteristics, this article aims to evaluate the potential of HDPs IDR1018 and DJK-6 associated with MTA extract in the repair process of human pulp cells. Antibacterial activity of HDPs, MTA and HDPs combined with MTA in Streptococcus mutans planktonic bacteria and antibiofilm activity was evaluated. Cell toxicity was assayed with MTT and cell morphology was observed by scanning electron microscopy (SEM). Proliferation and migration of pulp cells were evaluated by trypan blue and wound healing assay. Inflammatory and mineralization related genes were evaluated by qPCR (IL-6, TNFRSF, DSPP, TGF-β). Alkaline phosphatase, phosphate quantification and alizarin red staining were also verified. The assays were performed in technical and biological triplicate (n = 9). Results were submitted for the calculation of the mean and standard deviation. Then, normality verification by Kolmogorov Smirnov test, analyzing one-way ANOVA. Analyses were considered at a 95% significance level, with a p-value < 0.05. Our study demonstrated that HDPs combined with MTA were able to reduce biofilms performed in 24 h and biofilm performed over 7 days S. mutans biofilm (p < 0.05). IDR1018 and MTA, as well as their combination, down-regulated IL-6 expression (p < 0.05). Tested materials were not cytotoxic to pulp cells. IDR1018 induced high cell proliferation and combined with MTA induced high cellular migration rates in 48 h (p < 0.05). Furthermore, the combination of IDR1018 and MTA also induced high expression levels of DSPP, ALP activity, and the production of calcification nodules. So, IDR-1018 and its combination with MTA could assist in pulp-dentine complex repair process in vitro.
    MeSH term(s) Humans ; Dental Pulp ; Interleukin-6 ; Antimicrobial Cationic Peptides ; Alkaline Phosphatase ; Analysis of Variance ; Calcinosis
    Chemical Substances Interleukin-6 ; Antimicrobial Cationic Peptides ; Alkaline Phosphatase (EC 3.1.3.1)
    Language English
    Publishing date 2023-06-12
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-36748-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Concentrated MTA Repair HP reduced biofilm and can cause reparative action at a distance.

    Silva, Poliana Amanda Oliveira / Lima, Stella Maris de Freitas / Martins, Danilo César Mota / Amorim, Ingrid Aquino / Lacorte, Cristiano / de Almeida, Jeesser Alves / Franco, Octávio Luiz / Rezende, Taia Maria Berto

    International endodontic journal

    2021  Volume 54, Issue 10, Page(s) 1925–1936

    Abstract: Aim: To evaluate in vitro whether MTA Repair HP can induce repair processes at a distance, including its effects on biofilm, cell viability, migration, production of TGF-β, phosphate and ALP, evaluated through MTA diluted extracts.: Methodology: ... ...

    Abstract Aim: To evaluate in vitro whether MTA Repair HP can induce repair processes at a distance, including its effects on biofilm, cell viability, migration, production of TGF-β, phosphate and ALP, evaluated through MTA diluted extracts.
    Methodology: Initially, antibacterial tests were performed with the bacterium Streptococcus mutans (ATCC 25175) in the presence of MTA extracts (dilutions of 1:1, 1:2 and 1:4). Growth inhibition assay by microdilution in broth, antibiofilm plate assay of young biofilm and antibiofilm assay in confocal microscopy of mature biofilm were carried out. Then, pulp cells were stimulated in the presence of several MTA dilutions, and cell viability (MTT assay), proliferation and migration capacity (scratch assay) were evaluated. To evaluate the capacity of 1:1, 1:2 and 1:4 dilutions of MTA Repair HP to promote the production of important agents of odontogenic differentiation and mineralization, ALP activity, TGF-β secretion and phosphate quantification were measured. Statistical differences were verified using one-way and two-way anova and Tukey's post-tests.
    Results: The test dilutions of MTA Repair HP did not inhibit planktonic S. mutans growth but were able to reduce young and mature S. mutans biofilm (p < 0.001). In addition, none of the MTA Repair HP dilutions was cytotoxic for pulp cells. The 1:2 and 1:4 dilutions of MTA Repair HP induced migration and proliferation of pulp cells (p < 0.05). ALP activity and TGF-β secretion were independent of the tested dilution (p < 0.001). Diluted 1:4 MTA Repair HP produced less phosphate than the more concentrated 1:1 and 1:2 MTA dilutions (p < 0.001).
    Conclusions: Undiluted MTA Repair HP reduced S. mutans biofilm, when compared to 1:2 and 1:4 MTA dilutions. Furthermore, none of the tested dilutions was cytotoxic to pulp cells. MTA Repair HP promoted cell migration and proliferation at a distance, assessed through the dilution of the MTA. Even from a distance, MTA Repair HP has the ability to participate in some events related to repair, such as migration, proliferation and TGF production.
    MeSH term(s) Aluminum Compounds ; Biofilms ; Calcium Compounds/pharmacology ; Cells, Cultured ; Dental Pulp ; Drug Combinations ; Materials Testing ; Oxides/pharmacology ; Pemetrexed ; Root Canal Filling Materials ; Silicates/pharmacology
    Chemical Substances Aluminum Compounds ; Calcium Compounds ; Drug Combinations ; Oxides ; Root Canal Filling Materials ; Silicates ; Pemetrexed (04Q9AIZ7NO)
    Language English
    Publishing date 2021-07-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 603734-3
    ISSN 1365-2591 ; 0143-2885
    ISSN (online) 1365-2591
    ISSN 0143-2885
    DOI 10.1111/iej.13592
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 1508: Show issues Location:
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