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Article ; Online: A First Order q-Difference System for the BC_1-Type Jackson Integral and Its Applications

Masahiko Ito

Symmetry, Integrability and Geometry : Methods and Applications, Vol 5, p

2009 Volume 041

Abstract: We present an explicit expression for the q-difference system, which the BC_1-type Jackson integral (q-series) satisfies, as first order simultaneous q-difference equations with a concrete basis. As an application, we give a simple proof for the ... ...

Abstract	We present an explicit expression for the q-difference system, which the BC_1-type Jackson integral (q-series) satisfies, as first order simultaneous q-difference equations with a concrete basis. As an application, we give a simple proof for the hypergeometric summation formula introduced by Gustafson and the product formula of the q-integral introduced by Nassrallah-Rahman and Gustafson.
Keywords	q-difference equations ; Jackson integral of type BC_1 ; Gustafson's C_n-type sum ; Nassrallah-Rahman integral ; Mathematics ; QA1-939 ; Science ; Q ; DOAJ:Mathematics ; DOAJ:Mathematics and Statistics
Language	English
Publishing date	2009-04-01T00:00:00Z
Publisher	National Academy of Science of Ukraine
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Development and Use of a Kinetical and Real-Time Monitoring System to Analyze the Replication of Hepatitis C Virus

Xiaoyu Li / Masahiko Ito / Haruyo Aoyagi / Asako Murayama / Hideki Aizaki / Masayoshi Fukasawa / Takanobu Kato / Takaji Wakita / Tetsuro Suzuki

International Journal of Molecular Sciences, Vol 23, Iss 8711, p

2022 Volume 8711

Abstract: In microbiological research, it is important to understand the time course of each step in a pathogen’s lifecycle and changes in the host cell environment induced by infection. This study is the first to develop a real-time monitoring system that ... ...

Abstract	In microbiological research, it is important to understand the time course of each step in a pathogen’s lifecycle and changes in the host cell environment induced by infection. This study is the first to develop a real-time monitoring system that kinetically detects luminescence reporter activity over time without sampling cells or culture supernatants for analyzing the virus replication. Subgenomic replicon experiments with hepatitis C virus (HCV) showed that transient translation and genome replication can be detected separately, with the first peak of translation observed at 3–4 h and replication beginning around 20 h after viral RNA introduction into cells. From the bioluminescence data set measured every 30 min (48 measurements per day), the initial rates of translation and replication were calculated, and their capacity levels were expressed as the sums of the measured signals in each process, which correspond to the areas on the kinetics graphs. The comparison of various HuH-7-derived cell lines showed that the bioluminescence profile differs among cell lines, suggesting that both translation and replication capacities potentially influence differences in HCV susceptibility. The effects of RNA mutations within the 5′ UTR of the replicon on viral translation and replication were further analyzed in the system developed, confirming that mutations to the miR-122 binding sites primarily reduce replication activity rather than translation. The newly developed real-time monitoring system should be applied to the studies of various viruses and contribute to the analysis of transitions and progression of each process of their life cycle.
Keywords	hepatitis C virus ; genome replication ; bioluminescence profile ; real-time monitoring ; kinetical analysis ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	570
Language	English
Publishing date	2022-08-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Prevalence and Genetic Diversity of Staphylococcal Enterotoxin (-Like) Genes sey , selw , selx , selz , sel26 and sel27 in Community-Acquired Methicillin-Resistant Staphylococcus aureus

Meiji Soe Aung / Noriko Urushibara / Mitsuyo Kawaguchiya / Masahiko Ito / Satoshi Habadera / Nobumichi Kobayashi

Toxins, Vol 12, Iss 347, p

2020 Volume 347

Abstract: Staphylococcal enterotoxins (SEs) are virulence factors of Staphylococcus aureus associated with various toxic diseases due to their emetic and superantigenic activities. Although at least 27 SE(-like) genes have been identified in S. aureus to date, the ...

Abstract	Staphylococcal enterotoxins (SEs) are virulence factors of Staphylococcus aureus associated with various toxic diseases due to their emetic and superantigenic activities. Although at least 27 SE(-like) genes have been identified in S. aureus to date, the newly identified SE(-like) genes have not yet been well characterized by their epidemiological features. In this study, the prevalence and genetic diversity of SE gene sey and SE-like genes selw , selx , selz , sel26 , and sel27 were investigated for 624 clinical isolates of community-acquired methicillin-resistant S. aureus (CA-MRSA). The most prevalent SE(-like) gene was selw (92.9%), followed by selx (85.6%), sey (35.4%) and selz (5.6%), while sel26 and sel27 were not detected. Phylogenetically, sey , selw , selx , and selz were discriminated into 7, 10, 16, and 9 subtypes (groups), respectively. Among these subtypes, sey was the most conserved and showed the highest sequence identity (>98.8%), followed by selz and selx . The SE-like gene selw was the most divergent, and four out of ten genetic groups contained pseudogenes that may encode truncated product. Individual subtypes of SE(-like) genes were generally found in isolates with specific genotypes/lineages of S. aureus . This study revealed the putative ubiquity of selw and selx and the prevalence of sey and selz in some specific lineages (e.g., ST121) in CA-MRSA, suggesting a potential role of these newly described SEs(-like) in pathogenicity.
Keywords	Staphylococcus aureus ; enterotoxin ; sey ; selw ; selx ; selz ; Medicine ; R
Subject code	572
Language	English
Publishing date	2020-05-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Virus-like particles with FLAG-tagged envelope protein as a tetravalent dengue vaccine candidate

Toshifumi Imagawa / Masahiko Ito / Mami Matsuda / Kenji Nakashima / Yuhei Tokunaga / Isao Ohta / Tian-Cheng Li / Ryosuke Suzuki / Tetsuro Suzuki

Scientific Reports, Vol 11, Iss 1, Pp 1-

2021 Volume 10

Abstract: Abstract The global incidence of dengue, which is caused by dengue virus (DENV) infection, has grown dramatically in recent decades and secondary infection with heterologous serotype of the virus may cause severe symptoms. Efficacious dengue vaccines ... ...

Abstract	Abstract The global incidence of dengue, which is caused by dengue virus (DENV) infection, has grown dramatically in recent decades and secondary infection with heterologous serotype of the virus may cause severe symptoms. Efficacious dengue vaccines should be able to provide long-lasting immunity against all four DENV serotypes simultaneously. In this study, we constructed a novel vaccine platform based on tetravalent dengue virus-like particles (DENV-LPs) in which envelope (E) protein carried a FLAG tag sequence at the position located not only in the exterior loop on the protruding domain but outside of dimerization interface of the protein. We demonstrated an effective strategy to produce the DENV-LPs by transient transfection with expression plasmids for pre-membrane and E proteins of DENV-1 to DENV-4 in mammalian cells and to concentrate and purify them with one-step affinity chromatography. Characteristic features of VLPs such as particle size, shape and density were comparable to flavivirus-like particles reported. The neutralizing activity against all four DENV serotypes was successfully induced by immunization with the purified tetravalent VLPs in mice. Simple, one-step purification systems for VLP vaccine platforms using epitope-tagging strategy should be advantageous for vaccine development not only for dengue but for emerging pandemics in the future.
Keywords	Medicine ; R ; Science ; Q
Subject code	570
Language	English
Publishing date	2021-09-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Distribution of Virulence Factors and Resistance Determinants in Three Genotypes of Staphylococcus argenteus Clinical Isolates in Japan

Meiji Soe Aung / Noriko Urushibara / Mitsuyo Kawaguchiya / Mina Hirose / Miyo Ike / Masahiko Ito / Nobumichi Kobayashi

Pathogens, Vol 10, Iss 2, p

2021 Volume 163

Abstract: Staphylococcus argenteus , a novel staphylococcal species independent of S. aureus , causes a wide spectrum of infectious diseases. As detection of this species from humans and animals has been increasingly reported worldwide, its growing virulence and ... ...

Abstract	Staphylococcus argenteus , a novel staphylococcal species independent of S. aureus , causes a wide spectrum of infectious diseases. As detection of this species from humans and animals has been increasingly reported worldwide, its growing virulence and drug resistance via external genetic determinants has become concerning. In this study, the prevalence and genetic characteristics of virulence factors and drug resistance determinants were investigated for 82 S. argenteus clinical isolates in Hokkaido, Japan, for a one-year period starting in August 2019. These S. argenteus isolates corresponded to 0.66% of the total number of S. aureus isolates collected in the same period. The most prevalent genotype was sequence type (ST) 2250 and staphylocoagulase ( coa ) genotype XId (45.1%, n = 37), followed by ST1223- coa XV (30.5%, n = 25) and ST2198- coa XIV (24.4%, n = 20). Panton-Valentine leukocidin genes ( lukS-PV-lukF-PV ) were identified in a single ST2250 isolate. Only ST1223 isolates had the enterotoxin gene cluster ( egc-2 ), seb , and selw (detection rate; 100%, 60%, and 84%, respectively), while sec , sey , sel26-sel27 , tst-1 were only detected in ST2250 isolates (detection rate; 10.8%, 100%, 67.6%, and 10.8%, respectively). ST2198 isolates harbored selx at a significantly higher rate (60%) than isolates of other STs. Although most of S. argenteus isolates were susceptible to antimicrobials examined, ST2198 showed higher resistance rates to penicillin, macrolides, and aminoglycosides than other STs, and it harbored various resistance genes such as blaZ , erm(C) , msr(A) , lnuA , and aac(6′)-Ie-aph(2″)-Ia . Only one ST2250 isolate possessed SCC mec -IVc, showing resistance to oxacillin. blaZ was the most prevalent determinant of resistance in the three STs and belonged to two plasmid groups and a chromosomal group, suggesting its diverse origin. lnu(A) in ST2198 isolates was assigned to a major cluster with various staphylococcal species. The present study indicates that the prevalence of virulence factors and ...
Keywords	Staphylococcus argenteus ; ST ; virulence factors ; enterotoxin ; resistance gene ; Japan ; Medicine ; R
Language	English
Publishing date	2021-02-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Peripheral B cells as reservoirs for persistent HCV infection

ToshiakiMizuochi / MasahikoIto

Frontiers in Microbiology, Vol

2011 Volume 2

Keywords	B cell ; innate immunity ; HCV ; Microbiology ; QR1-502 ; Science ; Q
Language	English
Publishing date	2011-08-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
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Article ; Online: High prevalence of antimicrobial resistance in non-vaccine serotypes of non-invasive/colonization isolates of Streptococcus pneumoniae

Mitsuyo Kawaguchiya / Noriko Urushibara / Meiji Soe Aung / Masahiko Ito / Ayana Takahashi / Satoshi Habadera / Nobumichi Kobayashi

Journal of Infection and Public Health, Vol 13, Iss 8, Pp 1094-

A cross-sectional study eight years after the licensure of conjugate vaccine in Japan

2020 Volume 1100

Abstract: Background: Multidrug resistance (MDR) in non-vaccine serotypes (NVTs)-Streptococcus pneumoniae is a global public health concern after the widespread use of pneumococcal conjugate vaccines (PCVs). The present study aimed to analyze the prevalence of ... ...

Abstract	Background: Multidrug resistance (MDR) in non-vaccine serotypes (NVTs)-Streptococcus pneumoniae is a global public health concern after the widespread use of pneumococcal conjugate vaccines (PCVs). The present study aimed to analyze the prevalence of serotypes and antimicrobial susceptibilities of non-invasive/colonization isolates of S. pneumoniae eight years after the introduction of PCV in Japan. Methods: A total of 545 non-invasive pneumococcal isolates (460 children, 85 adults) obtained from July 2018 to January 2019 were studied. All isolates were tested for susceptibility to ten antimicrobials and were characterized for serotypes, penicillin binding protein (PBP) genotypes, and macrolide resistance genes. Results: Among children, 95.0% of isolates belonged to non-13-valent pneumococcal conjugate vaccine (PCV13) serotypes (NVTs), with 15A (15.0%) being dominant, followed by 35B (12.2%), 23A (11.1%), 15B (9.8%), and 15C (9.6%). In contrast, serotype 3 (18.8%) was the most prevalent in adults, while 15A, 10A, and 23A were also common. Serotypes covered by PCV13 and 23-valent pneumococcal polysaccharide vaccine (PPSV23) in all the adult isolates were 25.9% and 48.2%, respectively. High resistance rates were observed against erythromycin, tetracycline, and clindamycin (≥62.4% children, ≥58.8% adults). Penicillin-resistant and penicillin-intermediate isolates represented 2.2% and 33.8% of all isolates, respectively. Most isolates of the three dominant NVTs 15A, 35B, and 23A were non-susceptible to penicillin. Overall, multiple drug resistance (MDR) was detected in 69.0% of all isolates, including dominant NVTs 15A, 35B, 23A, 15B, and 15C. Among all the isolates, 81.8% harbored at least one altered PBP genes, and erm(B), mef(A/E) and both these genes were found in 68.2%, 23.3%, and 6.8%, respectively. High prevalence (>90%) of erm(B) was observed in serogroup 15, and serotypes 23A, 33F, and 12F. Conclusion: The present study revealed the high prevalence of NVTs 15 and 23A, and 35B showing MDR, suggesting ...
Keywords	Streptococcus pneumoniae ; Serotype ; Drug resistance ; Multidrug resistance (MDR) ; Pneumococcal conjugate vaccine (PCV) ; Japan ; Infectious and parasitic diseases ; RC109-216 ; Public aspects of medicine ; RA1-1270
Subject code	580
Language	English
Publishing date	2020-08-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Characterization of the self-assembly of New Jersey polyomavirus VP1 into virus-like particles and the virus seroprevalence in Japan

Xianfeng Zhou / Huimin Bai / Michiyo Kataoka / Masahiko Ito / Masamichi Muramatsu / Tetsuro Suzuki / Tian-Cheng Li

Scientific Reports, Vol 9, Iss 1, Pp 1-

2019 Volume 8

Abstract: Abstract New Jersey polyomavirus (NJPyV) was discovered in 2014 in a pancreatic transplant recipient’s vascular endothelial cells. Here, in the recombinant baculovirus system, VP1 protein of NJPyV expressed in insect cells was processed. The protein self- ...

Abstract	Abstract New Jersey polyomavirus (NJPyV) was discovered in 2014 in a pancreatic transplant recipient’s vascular endothelial cells. Here, in the recombinant baculovirus system, VP1 protein of NJPyV expressed in insect cells was processed. The protein self-assembled into virus-like particles (NJPyV-LPs) in a cell-type-dependent manner, and the particles were then released into the culture media. Spherical ~50-nm-dia. NJPyV-LPs of uniform size with morphology resembling that of the native particles of polyomaviruses were purified from the fraction at 1.33 g/cm3 in supernatants of VP1-expressing Sf9 cells. We investigated the antigenic properties of purified NJPyV-LPs and performed a VLP-based enzyme immunoassay to determine the age-specific prevalence of NJPyV infection in a general Japanese population aged 1–70 years. The overall seropositivity rate of anti-NJPyV antibodies was only 1.8%. This might be explained by the low circulation of NJPyV in Japan. This is the first report of a large-scale serological survey of NJPyV in Asia (n = 1,050).
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2019-09-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Molecular Epidemiological Characterization of Staphylococcus a rgenteus Clinical Isolates in Japan

Meiji Soe Aung / Noriko Urushibara / Mitsuyo Kawaguchiya / Ayako Sumi / Seika Takahashi / Miyo Ike / Masahiko Ito / Satoshi Habadera / Nobumichi Kobayashi

Microorganisms, Vol 7, Iss 10, p

Identification of Three Clones (ST1223, ST2198, and ST2550) and a Novel Staphylocoagulase Genotype XV

2019 Volume 389

Abstract: Staphylococcus argenteus , a novel emerging species within Staphylococcus aureus complex (SAC), has been increasingly reported worldwide. In this study, prevalence of S. argenteus among human clinical isolates, and their clonal diversity and genetic ... ...

Abstract	Staphylococcus argenteus , a novel emerging species within Staphylococcus aureus complex (SAC), has been increasingly reported worldwide. In this study, prevalence of S. argenteus among human clinical isolates, and their clonal diversity and genetic characteristics of virulence factors were investigated in Hokkaido, the northern main island of Japan. During a four-month period starting from March 2019, twenty-four S. argenteus and 4330 S. aureus isolates were recovered from clinical specimens (the ratio of S. argenteus to S. aureus :0.0055). Half of S. argenteus isolates ( n = 12) belonged to MLST sequence type (ST) 2250 and its single-locus variant, with staphylocoagulase genotype ( coa -) XId, while the remaining isolates were assigned to ST2198/ coa -XIV ( n = 6), and ST1223 with a novel coa -XV identified in this study ( n = 6). All the isolates were mecA -negative, and susceptible to all the antimicrobials tested, except for an ST2198 isolate with blaZ and an ST2250 isolate with tet (L) showing resistance to ampicillin and tetracyclines, respectively. Common virulence factors in the S. argenteus isolates were staphylococcal enterotoxin (-like) genes sey , selz , sel26 , and sel27 in ST2250, selx in ST2198, and enterotoxin gene cluster ( egc-1 : seg-sei- sem - sen-seo ) in ST1223 isolates, in addition to hemolysin genes ( hla , hlb , and hld ) distributed universally. Elastin binding protein gene ( ebpS ) and MSCRAMM family adhesin SdrE gene ( sdrE ) detected in all the isolates showed high sequence identity among them (> 97%), while relatively lower identity to those of S. aureus (78−92%). Phylogenetically, ebpS , sdrE , selx , sey , selw , sel26 , and sel27 of S. argenteus formed clusters distinct from those of S. aureus , unlike sec , selz , tst-1 , and staphylokinase gene ( sak ). The present study revealed the prevalence of S. argenteus among clinical isolates, and presence of three distinct S. argenteus clones (ST2250; ST2198 and ST1223) harboring different virulence factors in northern Japan. ...
Keywords	Staphylococcus argenteus ; ST ; staphyocoagulase genotype ; enterotoxin (-like) genes ; Japan ; Biology (General) ; QH301-705.5
Subject code	610 ; 580
Language	English
Publishing date	2019-09-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Partial silencing of fucosyltransferase 8 gene expression inhibits proliferation of Ishikawa cells, a cell line of endometrial cancer

Hana Shimoyama / Toshiaki K. Shibata / Masahiko Ito / Tomoaki Oda / Toshiya Itoh / Mari Mukai / Madoka Matsuya-Ogawa / Masashi Adachi / Hirotake Murakami / Takeshi Nakayama / Kazuhiro Sugihara / Hiroaki Itoh / Tetsuro Suzuki / Naohiro Kanayama

Biochemistry and Biophysics Reports, Vol 22, Iss , Pp - (2020)

2020

Abstract: Endometrial cancer is the most common gynecologic malignancy and is associated with increased morbidity each year, including young people. However, its mechanisms of proliferation and progression are not fully elucidated. It is well known that abnormal ... ...

Abstract	Endometrial cancer is the most common gynecologic malignancy and is associated with increased morbidity each year, including young people. However, its mechanisms of proliferation and progression are not fully elucidated. It is well known that abnormal glycosylation is involved in oncogenesis, and fucosylation is one of the most important types of glycosylation. In particular, fucosyltransferase 8 (FUT8) is the only FUT responsible for α1, 6-linked fucosylation (core fucosylation), and it is involved in various physiological as well as pathophysiological processes, including cancer biology. Therefore, we aimed to identify the expression of FUT8 in endometrial endometrioid carcinoma and investigate the effect of the partial silencing of the FUT8 gene on the cell proliferation of Ishikawa cells, an epithelial-like endometrial cancer cell line. Quantitative real-time PCR analysis showed that FUT8 gene expression was significantly elevated in the endometrial endometrioid carcinoma, compared to the normal endometrium. The immunostaining of FUT8 and Ulex europaeus Agglutinin 1 (UEA-1), a kind of lectin family specifically binding to fucose, was detected endometrial endometrioid carcinoma. The proliferation assay showed FUT8 partial knockdown by transfection of siRNA significantly suppressed the proliferation of Ishikawa cells, concomitant with the upregulation in the gene expressions associated with the interesting pathways associated with de-ubiquitination, aspirin trigger, mesenchymal-epithelial transition (MET) et al. It was suggested that the core fucosylation brought about by FUT8 might be involved in the proliferation of endometrial endometrioid carcinoma cells.
Keywords	Endometrial endometrioid carcinoma ; Fucosyltransferase 8 ; Ishikawa cells ; Cell proliferation ; xCELLigence ; Biology (General) ; QH301-705.5 ; Biochemistry ; QD415-436
Subject code	616
Language	English
Publishing date	2020-07-01T00:00:00Z
Publisher	Elsevier
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