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  1. Article ; Online: Functional capacity of natural killer cells in HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients.

    Queiroz, Gabriel Andrade Nonato / Mascarenhas, Rita Elizabeth Moreira / Vieillard, Vincent / Andrade, Raphaela Lisboa / Galvão-Castro, Bernardo / Grassi, Maria Fernanda Rios

    BMC infectious diseases

    2019  Volume 19, Issue 1, Page(s) 433

    Abstract: Background: Natural killer (NK) cells are part of the innate immune system and provide surveillance against viruses and cancers. The ability of NK cells to kill virus-infected cells depends on the balance between the effects of inhibitory and activating ...

    Abstract Background: Natural killer (NK) cells are part of the innate immune system and provide surveillance against viruses and cancers. The ability of NK cells to kill virus-infected cells depends on the balance between the effects of inhibitory and activating NK cell receptors. This study aimed to investigate the phenotypic profile and the functional capacity of NK cells in the context of HTLV-1 infection.
    Methods: This cross-sectional study sequentially recruited HTLV-1 infected individuals with HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) and asymptomatic HTLV-1 (AS) from the Integrated and Multidisciplinary HTLV Center in Salvador, Brazil. Blood samples from healthy blood donors served as controls. NK cell surface receptors (NKG2D, KIR2DL2/KIR2DL3, NKp30, NKG2A, NKp46, TIM-3 and PD-1), intracellular cytolytic (Granzyme B, perforin) and functional markers (CD107a for degranulation, IFN-γ) were assayed by flow cytometry in the presence or absence of standard K562 target cells. In addition, cytotoxicity assays were performed in the presence or absence of anti-NKp30.
    Results: The frequency of NKp30
    Conclusions: NK cells from individuals with a diagnosis of HAM/TSP present decreased expression of the activating receptor NKp30, in addition to elevated degranulation activity that remained unaffected after blocking the NKp30 receptor.
    MeSH term(s) Adult ; Antibodies, Monoclonal/pharmacology ; Biomarkers/metabolism ; Cross-Sectional Studies ; Female ; Flow Cytometry ; Granzymes/metabolism ; HTLV-I Infections/immunology ; HTLV-I Infections/virology ; Humans ; Interferon-gamma/metabolism ; K562 Cells ; Killer Cells, Natural/immunology ; Killer Cells, Natural/metabolism ; Killer Cells, Natural/virology ; Male ; Middle Aged ; Natural Cytotoxicity Triggering Receptor 3/antagonists & inhibitors ; Natural Cytotoxicity Triggering Receptor 3/metabolism ; Paraparesis, Tropical Spastic/immunology ; Paraparesis, Tropical Spastic/virology ; Perforin/metabolism
    Chemical Substances Antibodies, Monoclonal ; Biomarkers ; NCR3 protein, human ; Natural Cytotoxicity Triggering Receptor 3 ; Perforin (126465-35-8) ; Interferon-gamma (82115-62-6) ; GZMB protein, human (EC 3.4.21.-) ; Granzymes (EC 3.4.21.-)
    Language English
    Publishing date 2019-05-17
    Publishing country England
    Document type Journal Article
    ISSN 1471-2334
    ISSN (online) 1471-2334
    DOI 10.1186/s12879-019-4032-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test.

    Silva, Bosco Christiano Maciel da / Grassi, Maria Fernanda Rios / Coutinho, Raimundo / Mascarenhas, Rita Elizabeth Moreira / Olavarria, Viviana Nilla / Coutinho-Borgo, Adriana / Kalil, Jorge / Cunha Neto, Edecio / Fonseca, Simone Gonçalves

    Memorias do Instituto Oswaldo Cruz

    2014  Volume 109, Issue 8, Page(s) 999–1004

    Abstract: The interferon (IFN)-γ response to peptides can be a useful diagnostic marker of Mycobacterium tuberculosis (MTB) latent infection. We identified promiscuous and potentially protective CD4+ T-cell epitopes from the most conserved regions of MTB antigenic ...

    Abstract The interferon (IFN)-γ response to peptides can be a useful diagnostic marker of Mycobacterium tuberculosis (MTB) latent infection. We identified promiscuous and potentially protective CD4+ T-cell epitopes from the most conserved regions of MTB antigenic proteins by scanning the MTB antigenic proteins GroEL2, phosphate-binding protein 1 precursor and 19 kDa antigen with the TEPITOPE algorithm. Seven peptide sequences predicted to bind to multiple human leukocyte antigen (HLA)-DR molecules were synthesised and tested with IFN-γ enzyme-linked immunospot (ELISPOT) assays using peripheral blood mononuclear cells (PBMCs) from 16 Mantoux tuberculin skin test (TST)-positive and 16 TST-negative healthy donors. Eighty-eight percent of TST-positive donors responded to at least one of the peptides, compared to 25% of TST-negative donors. Each individual peptide induced IFN-γ production by PBMCs from at least 31% of the TST-positive donors. The magnitude of the response against all peptides was 182 ± 230 x 106 IFN-γ spot forming cells (SFC) among TST-positive donors and 36 ± 62 x 106 SFC among TST-negative donors (p = 0.007). The response to GroEL2 (463-477) was only observed in the TST-positive group. This combination of novel MTB CD4 T-cell epitopes should be tested in a larger cohort of individuals with latent tuberculosis (TB) to evaluate its potential to diagnose latent TB and it may be included in ELISPOT-based IFN-γ assays to identify individuals with this condition.
    MeSH term(s) Adult ; Algorithms ; Antigens, Bacterial/analysis ; Bacterial Proteins/blood ; Biomarkers/analysis ; Brazil ; CD4-Positive T-Lymphocytes/immunology ; CD4-Positive T-Lymphocytes/metabolism ; Chaperonins/blood ; Enzyme-Linked Immunospot Assay ; Epitope Mapping ; Epitopes/immunology ; HLA-DR Antigens/immunology ; Healthy Volunteers ; Humans ; Interferon-gamma/metabolism ; Latent Tuberculosis/diagnosis ; Latent Tuberculosis/immunology ; Leukocytes, Mononuclear/immunology ; Leukocytes, Mononuclear/metabolism ; Middle Aged ; Mycobacterium tuberculosis/immunology ; Phosphate-Binding Proteins/blood ; Tuberculin Test
    Chemical Substances Antigens, Bacterial ; Bacterial Proteins ; Biomarkers ; Epitopes ; GroESL protein, Bacteria ; HLA-DR Antigens ; Phosphate-Binding Proteins ; Interferon-gamma (82115-62-6) ; Chaperonins (EC 3.6.1.-)
    Language English
    Publishing date 2014-12
    Publishing country Brazil
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 953293-6
    ISSN 1678-8060 ; 0074-0276
    ISSN (online) 1678-8060
    ISSN 0074-0276
    DOI 10.1590/0074-0276140193
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Ud II Zs.33: Show issues Location:
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  3. Article ; Online: Prevalence and risk factors for bacterial vaginosis and other vulvovaginitis in a population of sexually active adolescents from Salvador, Bahia, Brazil.

    Mascarenhas, Rita Elizabeth Moreira / Machado, Márcia Sacramento Cunha / Costa e Silva, Bruno Fernando Borges da / Pimentel, Rodrigo Fernandes Weyll / Ferreira, Tatiana Teixeira / Leoni, Fernanda Maria Silva / Grassi, Maria Fernanda Rios

    Infectious diseases in obstetrics and gynecology

    2012  Volume 2012, Page(s) 378640

    Abstract: Bacterial vaginosis, trichomoniasis, and genital candidiasis are considered the main etiologies of vulvovaginitis. Few studies estimate the prevalence of vulvovaginitis among adolescents, especially in Brazil. This study aimed to determine the prevalence ...

    Abstract Bacterial vaginosis, trichomoniasis, and genital candidiasis are considered the main etiologies of vulvovaginitis. Few studies estimate the prevalence of vulvovaginitis among adolescents, especially in Brazil. This study aimed to determine the prevalence and main risk factors associated with bacterial vaginosis and genital infection by C. albicans and Trichomonas vaginalis among a group of adolescents from Salvador, Bahia, Brazil. One hundred sexually active adolescents followed at an adolescent gynecology clinic were included. Endocervical and vaginal samples were obtained during gynecological examination. Nugent criteria were applied for the diagnosis of bacterial vaginosis. For Candida albicans and Trichomonas vaginalis detection, culture in Sabouraud agar plates and Papanicolaou cytology were used, respectively. The mean age of participants was 16.6 ± 1.6 years. The prevalence of bacterial vaginosis was 20% (95% CI 12-28) and of genital infection by Candida was 22% (95% CI 14-30). Vaginal cytology detected Trichomonas vaginalis in one patient. Alcohol, tobacco, and illegal drug use (P = 0.02) and multiple lifetime partners were statistically related to bacterial vaginosis (P = 0.01). The prevalence of bacterial vaginosis and genital candidiasis was similar to other studies carried out among adolescents worldwide.
    MeSH term(s) Adolescent ; Brazil/epidemiology ; Candidiasis, Vulvovaginal/epidemiology ; Female ; Humans ; Prevalence ; Risk Factors ; Sexual Behavior/statistics & numerical data ; Sexual Partners ; Substance-Related Disorders/epidemiology ; Vaginosis, Bacterial/epidemiology
    Language English
    Publishing date 2012-10-22
    Publishing country Egypt
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1176776-5
    ISSN 1098-0997 ; 1064-7449
    ISSN (online) 1098-0997
    ISSN 1064-7449
    DOI 10.1155/2012/378640
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 3915: Show issues Location:
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