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Article ; Online: Protein-polysaccharide based double network microbeads improves stability of Bifidobacterium infantis ATCC 15697 in a gastro-Intestinal tract model (TIM-1).

Khan, Wahab Ali / Butt, Masood Sadiq / Yasmin, Iqra / Wadood, Syed Abdul / Mahmood, Asif / Gad, Heba A

2024 Volume 652, Page(s) 123804

Abstract: Microencapsulation of probiotics is a main technique employed to improve cell survival in gastrointestinal tract (GIT). The present study investigated the impact of utilizing proteins i.e. Whey Protein Isolates (WPI), Pea Protein Isolates (PPI) or (WPI + ...

Abstract	Microencapsulation of probiotics is a main technique employed to improve cell survival in gastrointestinal tract (GIT). The present study investigated the impact of utilizing proteins i.e. Whey Protein Isolates (WPI), Pea Protein Isolates (PPI) or (WPI + PPI) complex based microbeads as encapsulating agents on the encapsulation efficiency (EE), diameter, morphology along with the survival and viability of Bifidobacterium infantis ATCC 15697. Results revealed that WPI + PPI combination had the highest EE% of the probiotics up to 94.09 % and the smoothest surface with less visible holes. WPI based beads revealed lower EE% and smaller size than PPI based ones. In addition, WPI based beads showed rough surface with visible signs of cracks, while PPI beads showed dense surfaces with pores and depressions. In contrast, the combination of the two proteins resulted in compact and smooth beads with less visible pores/wrinkles. The survival in gastrointestinal tract (GIT) was observed through TNO in-vitro gastrointestinal model (TIM-1) and results illustrated that all microbeads shrank in gastric phase while swelled in intestinal phase. In addition, in-vitro survival rate of free cells was very low in gastric phase (18.2 %) and intestinal phase (27.5 %). The free cells lost their viability after 28 days of storage (2.66 CFU/mL) with a maximum log reduction of 6.76, while all the encapsulated probiotic showed more than 10
MeSH term(s)	Bifidobacterium longum subspecies infantis ; Microspheres ; Gastrointestinal Tract ; Polysaccharides ; Whey Proteins ; Probiotics ; Microbial Viability
Chemical Substances	Polysaccharides ; Whey Proteins
Language	English
Publishing date	2024-01-12
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	428962-6
ISSN	1873-3476 ; 0378-5173
ISSN (online)	1873-3476
ISSN	0378-5173
DOI	10.1016/j.ijpharm.2024.123804
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Article: Phytochemical screening of

Saeed, Raakia Anam / Khan, Muhammad Issa / Butt, Masood Sadiq / Faisal, Muhammad Naeem

Frontiers in nutrition

2023 Volume 10, Page(s) 1132356

Abstract: Scope: Prunus avium: Methods and results: The quantification of ultrasound-assisted extract (UAE) of : Conclusion: Consequently, the polyphenol-rich sweet cherries UAE exhibited antiproliferative and chemo-preventive potential by reducing tumor ... ...

Abstract	Scope: Prunus avium Methods and results: The quantification of ultrasound-assisted extract (UAE) of Conclusion: Consequently, the polyphenol-rich sweet cherries UAE exhibited antiproliferative and chemo-preventive potential by reducing tumor biomarkers, serum transaminases and oxidative stress, as well as enhancing antioxidant status. It further upregulated the downstream targets of ATM signaling cascade.
Language	English
Publishing date	2023-05-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2776676-7
ISSN	2296-861X
ISSN	2296-861X
DOI	10.3389/fnut.2023.1132356
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Article ; Online: INDEXED STROKE VOLUME IN CHILDREN WITH VARYING LEFT VENTRICLE EJECTION FRACTION AND ITS CORRELATION WITH VARIOUS CARDIAC FACTORS

Rizwan Ullah Jan / Ahmad Usaid Qureshi / Syed Najam Hyder / Masood Sadiq

Khyber Medical University Journal, Vol 15, Iss 1, Pp 31-

2023 Volume 7

Abstract: OBJECTIVE: To determine the variation in indexed stroke volume (LVSVi) in children with varying left ventricle ejection fraction (LVEF) using cardiac magnetic imaging (CMR) and its correlation with various cardiac factors. METHODS: This observational ... ...

Abstract	OBJECTIVE: To determine the variation in indexed stroke volume (LVSVi) in children with varying left ventricle ejection fraction (LVEF) using cardiac magnetic imaging (CMR) and its correlation with various cardiac factors. METHODS: This observational comparative study was conducted at The Children’s Hospital, Lahore, Pakistan from December 2018 to November 2021. All children below 18 years’ age presenting to hospital for CMR for tissue characterization, having normal vital organs function and no clinical signs of heart failure were included in the study. Relevant clinical data was recorded. CMR was performed using 1.5T Philips Ingenia MRI scanner. The data were analyzed with varying LVEF and correlation of LVSVi with various cardiac factors including indexed left ventricular end diastolic volume (LVEDVi), cardiac output (CO) and heart rate (HR). RESULTS: Out of 175 patients, 170 children up to 18 years old completed the test with mean age 14.3±3.3 years. Mean LVSVi was 42+12 ml/m2 which followed Frank Starling curve except in children with LVEF <36%. Mean LVEDVi was 86±34 ml/m2. LVSVi did not correlate with heart rate or indexed ventricular systolic volumes acting as an independent variable. Minimum LVSVi remained similar all groups as demonstrated through centile distribution. CONCLUSION: Indexed stroke volume is an independent variable in children having normal vital organs function with varying LVEF. It can serve as an independent monitoring parameter for clinical management of children with impaired ejection fraction.
Keywords	stroke volume ; blood ; heart ; ventricular function ; cardiac output ; high ; low ; child ; preschool ; cardiac mri ; cardiac imaging techniques ; magnetic resonance imaging ; Medicine ; R
Subject code	610 ; 616
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	Khyber Medical University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Fabrication and characterization of carnauba wax-based films incorporated with sodium alginate/whey protein

Butt, Masood Sadiq / Akhtar, Muhammad / Maan, Abid Aslam / Asghar, Muhammad

Food Measure. 2023 Feb., v. 17, no. 1 p.694-705

2023

Abstract: Edible films and coatings developed through biopolymers have good tensile strength and gaseous barriers but lack moisture barrier properties. To improve the moisture barrier properties of sodium alginate and whey protein-based films, carnauba wax at ... ...

Abstract	Edible films and coatings developed through biopolymers have good tensile strength and gaseous barriers but lack moisture barrier properties. To improve the moisture barrier properties of sodium alginate and whey protein-based films, carnauba wax at different concentrations (0.5, 1.0, and 1.5%) was incorporated. Results showed significant variations as the concentration of the carnauba wax increased. The addition of wax at a higher concentration (1.5%) significantly improved the moisture barrier properties (3.12 ± 0.31 g.mm/kPa.h.m²). However, tensile strength reduced (3.92 ± 0.04 to 3.34 ± 0.04 MPa) with the increase in wax concentration. The incorporation of lipid constituents in the emulsion-based films also affects the physicochemical properties including thickness, droplet size, transparency, and microstructure of the films as well. At lower concentrations (0.5%) films showed uniform microstructure and better transparency (81.82 ± 1.63%) but lack in moisture barrier properties. Emulsified edible films with improved moisture barrier and tensile properties are preferable in the food packaging industry. Emulsion-based films and coatings have potential applications in foods, especially in fresh and processed foods, dairy, bakery products, meat, and sausages.
Keywords	biopolymers ; carnauba wax ; droplet size ; industry ; lipids ; meat ; microstructure ; sodium alginate ; tensile strength ; whey ; whey protein
Language	English
Dates of publication	2023-02
Size	p. 694-705.
Publishing place	Springer US
Document type	Article ; Online
ZDB-ID	2754612-3
ISSN	2193-4134 ; 2193-4126
ISSN (online)	2193-4134
ISSN	2193-4126
DOI	10.1007/s11694-022-01636-3
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Article ; Online: Co-supplementation of Zinc and Calcium Suppresses Bio-absorption of Lead in Sprague Dawley Rats

Butt, Masood Sadiq / Iahtisham-Ul-Haq / Javed, Komal / Tariq, Urwa

Biol Trace Elem Res. 2023 Mar., v. 201, no. 3 p.1317-1326

2023

Abstract: Lead (Pb) is a widespread environmental toxicant and its toxicity causes huge health impacts. The present study was conducted to examine the protective role of zinc (Zn) and calcium (Ca) supplements against bio-absorption of Pb in blood and organs ... ...

Abstract	Lead (Pb) is a widespread environmental toxicant and its toxicity causes huge health impacts. The present study was conducted to examine the protective role of zinc (Zn) and calcium (Ca) supplements against bio-absorption of Pb in blood and organs including the liver and kidney. Hence, Sprague Dawley rats were divided in to five groups. G₁ served as negative control and was provided with standard diet, G₂ as positive control receiving standard diet + PbAc (20 mg/kg BW), G₃ was provided with standard diet + PbAc (20 mg/kg BW) + ZnSO₄ (20 mg/kg BW), G₄ with standard diet + PbAc (20 mg/kg BW) + CaCO₃ (7.5 g/kg BW) whereas G₅ was fed on standard diet + PbAc (20 mg/kg BW) + ZnSO₄ (20 mg/kg BW) + CaCO₃ (7.5 g/kg BW). The salts were provided as solution, dissolved in 0.5 mL distilled water via orogastric tube. After 35 days, the overnight fasted rats were decapitated, and blood and organs were collected for analysis of levels of metals and liver and kidney function tests. The results depicted significant decrease in Pb concentration in blood and organs while increase in Zn and Ca absorption was observed as a result of Zn and Ca supplementation with Zn being better than Ca alone, specially however, combined effect of these supplements was more profound in improving liver and kidney stress biomarkers and maintained the normal architecture of renal and hepatic parenchyma. It was concluded that Zn and Ca co-supplementation hinder Pb absorption in blood, the liver, and kidney thus suggesting that their intake may protect from Pb toxicity.
Keywords	absorption ; biomarkers ; blood ; calcium ; diet ; kidneys ; liver ; protective effect ; renal function ; toxicity ; zinc
Language	English
Dates of publication	2023-03
Size	p. 1317-1326.
Publishing place	Springer US
Document type	Article ; Online
ZDB-ID	445336-0
ISSN	1559-0720 ; 0163-4984
ISSN (online)	1559-0720
ISSN	0163-4984
DOI	10.1007/s12011-022-03233-3
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Article: Therapeutic efficacy of spirulina against ovalbumin and cigarette smoke-induced asthma-specific stress biomarkers in Sprague-Dawley rats.

Riaz, Khadija / Butt, Masood Sadiq / Sharif, Mian Kamran / Faisal, Muhammad Naeem

Food science & nutrition

2022 Volume 11, Issue 2, Page(s) 972–982

Abstract: Due to the high prevalence of allergies and asthma, awareness about allergens and therapeutic use of functional foods and nutraceuticals have gained immense attention. Spirulina powder is being used as health-boosting and antioxidant agent against ... ...

Abstract	Due to the high prevalence of allergies and asthma, awareness about allergens and therapeutic use of functional foods and nutraceuticals have gained immense attention. Spirulina powder is being used as health-boosting and antioxidant agent against several ailments owing to its unique nutritional profile. Considering its antioxidant role, the current study was focused on exploring therapeutic role of spirulina against stress biomarkers in asthmatic model. To assess the therapeutic efficacy of spirulina against allergic asthma-specific oxidative stress biomarkers, a model feed trial was conducted and rats were divided into four groups (
Language	English
Publishing date	2022-11-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	2703010-6
ISSN	2048-7177
ISSN	2048-7177
DOI	10.1002/fsn3.3132
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Article ; Online: Co-supplementation of Zinc and Calcium Suppresses Bio-absorption of Lead in Sprague Dawley Rats.

Butt, Masood Sadiq / Iahtisham-Ul-Haq / Javed, Komal / Tariq, Urwa

Biological trace element research

2022 Volume 201, Issue 3, Page(s) 1317–1326

Abstract	Lead (Pb) is a widespread environmental toxicant and its toxicity causes huge health impacts. The present study was conducted to examine the protective role of zinc (Zn) and calcium (Ca) supplements against bio-absorption of Pb in blood and organs including the liver and kidney. Hence, Sprague Dawley rats were divided in to five groups. G
MeSH term(s)	Rats ; Animals ; Zinc/pharmacology ; Zinc/analysis ; Calcium/analysis ; Rats, Sprague-Dawley ; Lead/toxicity ; Dietary Supplements ; Calcium, Dietary
Chemical Substances	Zinc (J41CSQ7QDS) ; Calcium (SY7Q814VUP) ; Lead (2P299V784P) ; Calcium, Dietary
Language	English
Publishing date	2022-04-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	445336-0
ISSN	1559-0720 ; 0163-4984
ISSN (online)	1559-0720
ISSN	0163-4984
DOI	10.1007/s12011-022-03233-3
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Article ; Online: Prophylactic role of olive fruit extract against cigarette smoke-induced oxidative stress in Sprague-Dawley rats.

Tariq, Urwa / Butt, Masood Sadiq / Pasha, Imran / Faisal, Muhammad Naeem

Cell stress & chaperones

2022 Volume 27, Issue 5, Page(s) 545–560

Abstract: Cigarette smoke exposure increases the production of free radicals leading to initiation of several pathological conditions by triggering the oxidative stress and inflammatory cascade. Olive fruit owing to its unique phytochemical composition possesses ... ...

Abstract	Cigarette smoke exposure increases the production of free radicals leading to initiation of several pathological conditions by triggering the oxidative stress and inflammatory cascade. Olive fruit owing to its unique phytochemical composition possesses antioxidant, immune modulatory, and anti-inflammatory potential. Considering the compositional alterations in olive fruits during ripening, the current experimental trail was designed to investigate the prophylactic role of green and black olives against the oxidative stress induced by cigarette smoke exposure in rats. Purposely, rats were divided into five different groups: NC (negative control; normal diet), PC [positive control; normal diet + smoke exposure (SE)], drug (normal diet + SE + citalopram), GO (normal diet + SE + green olive extract), and BO (normal diet + SE + black olive extract). Rats of all groups were exposed to cigarette smoke except "NC" and were sacrificed for collection of blood and organs after 28 days of experimental trial. The percent reduction in total oxidative stress by citalopram and green and black olive extracts in serum was 29.72, 58.69, and 57.97%, respectively, while the total antioxidant capacity increased by 30.78, 53.94, and 43.98%, accordingly in comparison to PC. Moreover, malondialdehyde (MDA) was reduced by 29.63, 42.59, and 45.70% in drug, GO, and BO groups, respectively. Likewise, green and black olive extracts reduced the leakage of hepatic enzymes in sera, alkaline phosphatase (ALP) by 23.44 and 25.80% and 35.62 and 37.61%, alanine transaminase (ALT) by 42.68 and 24.39% and 51.04 and 35.41%, and aspartate transaminase (AST) by 31.51 and 16.07% and 40.50 and 27.09% from PC and drug group, respectively. Additionally, olive extracts also maintained the antioxidant pool, i.e., superoxide dismutase, catalase, and glutathione in serum. Furthermore, histological examination revealed that olive extracts prevented the cigarette smoke-induced necrosis, pyknotic alterations, and congestion in the lung, hepatic, and renal parenchyma. Besides, gene expression analysis revealed that olive extracts and citalopram decreased the brain and lung damage caused by stress-induced upregulation of NRF-2 and MAPK signaling pathways. Hence, it can be concluded that olives (both green and black) can act as promising antioxidant in alleviating the cigarette smoke-induced oxidative stress.
MeSH term(s)	Alanine Transaminase ; Alkaline Phosphatase/metabolism ; Alkaline Phosphatase/pharmacology ; Animals ; Anti-Inflammatory Agents/pharmacology ; Antioxidants/metabolism ; Antioxidants/pharmacology ; Aspartate Aminotransferases ; Biological Products ; Catalase/metabolism ; Cigarette Smoking ; Citalopram/metabolism ; Citalopram/pharmacology ; Fruit ; Glutathione/metabolism ; Liver/metabolism ; Malondialdehyde/metabolism ; Olea/metabolism ; Oxidative Stress ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Rats ; Rats, Sprague-Dawley ; Superoxide Dismutase/metabolism
Chemical Substances	Anti-Inflammatory Agents ; Antioxidants ; Biological Products ; Plant Extracts ; olive extract ; Citalopram (0DHU5B8D6V) ; Malondialdehyde (4Y8F71G49Q) ; Catalase (EC 1.11.1.6) ; Superoxide Dismutase (EC 1.15.1.1) ; Aspartate Aminotransferases (EC 2.6.1.1) ; Alanine Transaminase (EC 2.6.1.2) ; Alkaline Phosphatase (EC 3.1.3.1) ; Glutathione (GAN16C9B8O)
Language	English
Publishing date	2022-08-11
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1362749-1
ISSN	1466-1268 ; 1355-8145
ISSN (online)	1466-1268
ISSN	1355-8145
DOI	10.1007/s12192-022-01291-z
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Article ; Online: Probing the antioxidant potential of Juglans regia (walnut) against arthritis-induced oxidative stress in Sprague Dawley rats.

Javed, Komal / Rakha, Allah / Butt, Masood Sadiq / Faisal, Muhammad Naeem

Journal of food biochemistry

2022 Volume 46, Issue 5, Page(s) e14082

Abstract: Oxidative stress is the underlying cause of various chronic diseases and can contribute to its progression. Plant-based therapeutics are effective in the prevention of various chronic diseases through the management of underlying causes. Walnuts owing to ...

Abstract	Oxidative stress is the underlying cause of various chronic diseases and can contribute to its progression. Plant-based therapeutics are effective in the prevention of various chronic diseases through the management of underlying causes. Walnuts owing to their rich phytochemistry possess antioxidative potential. In this context, the present research was designed to explore the therapeutic potential of walnut against arthritis-induced oxidative stress in rat model. Purposely, 50 Sprague Dawley rats were separated into five groups of 10 rats each. The rats were categorized as G
MeSH term(s)	Animals ; Antioxidants/pharmacology ; Arthritis ; Juglans/chemistry ; Oxidative Stress ; Plant Extracts/pharmacology ; Rats ; Rats, Sprague-Dawley
Chemical Substances	Antioxidants ; Plant Extracts
Language	English
Publishing date	2022-01-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	433846-7
ISSN	1745-4514 ; 0145-8884
ISSN (online)	1745-4514
ISSN	0145-8884
DOI	10.1111/jfbc.14082
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Article: Development and characterization of emulsion-based films incorporated with chitosan and sodium caseinate

Akhtar, Muhammad / Butt, Masood Sadiq / Maan, Abid Aslam / Asghar, Muhammad

Journal of food measurement & characterization. 2022 Aug., v. 16, no. 4

2022

Abstract: This work analyses the impact of lipid addition (beeswax) on the physicochemical, rheological, microstructural, mechanical, and moisture barrier properties of the chitosan/sodium caseinate composite solution. The addition of lipid affects the different ... ...

Abstract	This work analyses the impact of lipid addition (beeswax) on the physicochemical, rheological, microstructural, mechanical, and moisture barrier properties of the chitosan/sodium caseinate composite solution. The addition of lipid affects the different parameters but improves the moisture barrier properties of the films in comparison to the films without lipid addition. The addition of lipid influences the rheological and microstructural properties as higher concentration results in more viscous solutions and films with a rough surface and larger droplets size. Film thickness and particle size increased as the concentration of beeswax increased from 0.5% to 1.5% in the film-forming solution. Transparency of the film is also influenced by the addition of lipids in the emulsion but the concentration of other film-forming constituents affects the transparency of the films. Mechanical properties of the films are inversely related to the increase in wax concentration as tensile strength and elongation at breakage decreased as the concentration of wax increased, however, young’s modulus has not been affected. Biodegradable edible films with improved moisture barrier and tensile properties are preferable in the food packaging industry as these can help in enhancing the package quality and shelf stability of foods especially mangoes, strawberries, bananas, and citrus fruits.
Keywords	Citrus ; beeswax ; biodegradability ; chitosan ; emulsions ; industry ; lipids ; measurement ; particle size ; sodium caseinate ; tensile strength
Language	English
Dates of publication	2022-08
Size	p. 3278-3288.
Publishing place	Springer US
Document type	Article
ZDB-ID	2754612-3
ISSN	2193-4134 ; 2193-4126
ISSN (online)	2193-4134
ISSN	2193-4126
DOI	10.1007/s11694-022-01422-1
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