Article ; Online: Evaluation of post-natal angiogenesis in a mouse hind limb ischemia model
STAR Protocols, Vol 4, Iss 2, Pp 102232- (2023)
2023
Abstract: Summary: Hind limb ischemia is a useful model to assess metabolic and cellular responses. Here, we present a protocol for evaluating post-natal angiogenesis in a mouse hind limb ischemia model. We describe steps to induce a severe restriction of blood ... ...
| Abstract | Summary: Hind limb ischemia is a useful model to assess metabolic and cellular responses. Here, we present a protocol for evaluating post-natal angiogenesis in a mouse hind limb ischemia model. We describe steps to induce a severe restriction of blood supply of the femoral artery and vein that mimics the real-life scenario observed in clinical settings. We then detail procedures for follow-up laser Doppler imaging to compare post-ischemic responses of four different mouse strains in their capacity to trigger compensatory arteriogenesis.For complete details on the use and execution of this protocol, please refer to Oberkersch et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
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| Keywords | Health Sciences ; Metabolism ; Microscopy ; Model Organisms ; Science (General) ; Q1-390 |
| Language | English |
| Publishing date | 2023-06-01T00:00:00Z |
| Publisher | Elsevier |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
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