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  1. AU="Matczuk, Anna Karolina"
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  1. Article ; Online: Molecular detection of avian hepatitis E virus (Orthohepevirus B) in chickens, ducks, geese, and western capercaillies in Poland.

    Siedlecka, Magdalena / Kublicka, Agata / Wieliczko, Alina / Matczuk, Anna Karolina

    PloS one

    2022  Volume 17, Issue 6, Page(s) e0269854

    Abstract: Orthohepevirus B, commonly known as avian hepatitis E virus (aHEV), causes big liver and spleen disease (BLS) or hepatitis-splenomegaly syndrome (HSS) in chickens. BLS is an emerging disease among chicken flocks in several countries around the world. In ... ...

    Abstract Orthohepevirus B, commonly known as avian hepatitis E virus (aHEV), causes big liver and spleen disease (BLS) or hepatitis-splenomegaly syndrome (HSS) in chickens. BLS is an emerging disease among chicken flocks in several countries around the world. In our previous studies, serology and molecular biology screening revealed that chicken flocks are widely affected by aHEV in Poland. The present study, which was conducted between 2019 and 2020, aimed to investigate the prevalence of aHEV in chicken flocks and other poultry, including ducks, geese, and turkeys. A total of 307 flocks were examined. In addition, 29 samples from captive wild birds (western capercaillies, Tetrao urogallus) were analyzed. In all the investigated poultry species, except turkeys, the nucleic acid sequence covering part of the ORF1 gene of the aHEV genome was detected (34/336 samples, 10.1%). The infection rate was found to be the highest in broiler breeder chicken flocks (14/40 samples; 35%). Phylogenetic analysis of partial ORF1 gene, which encodes helicase, revealed that the obtained sequences belonged to genotypes 2 and 4, while one belonged to genotype 3. Genotype 2 was detected for the first time in domestic geese and ducks, and genotype 4 was detected for the first time in Poland. The study demonstrated the presence of aHEV among the investigated western capercaillies, suggesting that this species is susceptible to aHEV infections and biosecurity is therefore required in western capercaillie breeding facilities.
    MeSH term(s) Animals ; Chickens ; Ducks ; Geese ; Hepatitis, Viral, Animal/epidemiology ; Hepevirus ; Phylogeny ; Poland/epidemiology ; Poultry Diseases/epidemiology ; Quail ; Turkeys
    Language English
    Publishing date 2022-06-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0269854
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Expression of the Heterotrimeric GP2/GP3/GP4 Spike of an Arterivirus in Mammalian Cells.

    Matczuk, Anna Karolina / Zhang, Minze / Veit, Michael / Ugorski, Maciej

    Viruses

    2022  Volume 14, Issue 4

    Abstract: Equine arteritis virus (EAV), an enveloped positive-strand RNA virus, is an important pathogen of horses and the prototype member of the Arteiviridae family. Unlike many other enveloped viruses, which possess homotrimeric spikes, the spike responsible ... ...

    Abstract Equine arteritis virus (EAV), an enveloped positive-strand RNA virus, is an important pathogen of horses and the prototype member of the Arteiviridae family. Unlike many other enveloped viruses, which possess homotrimeric spikes, the spike responsible for cellular tropism in Arteriviruses is a heterotrimer composed of 3 glycoproteins: GP2, GP3, and GP4. Together with the hydrophobic protein E they are the minor components of virus particles. We describe the expression of all 3 minor glycoproteins, each equipped with a different tag, from a multi-cassette system in mammalian BHK-21 cells. Coprecipitation studies suggest that a rather small faction of GP2, GP3, and GP4 form dimeric or trimeric complexes. GP2, GP3, and GP4 co-localize with each other and also, albeit weaker, with the E-protein. The co-localization of GP3-HA and GP2-myc was tested with markers for ER, ERGIC, and cis-Golgi. The co-localization of GP3-HA was the same regardless of whether it was expressed alone or as a complex, whereas the transport of GP2-myc to cis-Golgi was higher when this protein was expressed as a complex. The glycosylation pattern was also independent of whether the proteins were expressed alone or together. The recombinant spike might be a tool for basic research but might also be used as a subunit vaccine for horses.
    MeSH term(s) Animals ; Arterivirus ; Equartevirus/genetics ; Equartevirus/metabolism ; Glycoproteins/genetics ; Guanidines ; Horses ; Mammals ; Piperazines ; Viral Envelope Proteins/metabolism
    Chemical Substances Glycoproteins ; Guanidines ; Piperazines ; Viral Envelope Proteins ; GP 4 (81746-15-8)
    Language English
    Publishing date 2022-04-01
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14040749
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Quantifying the Dynamics of Bacterial Biofilm Formation on the Surface of Soft Contact Lens Materials Using Digital Holographic Tomography to Advance Biofilm Research.

    Buzalewicz, Igor / Kaczorowska, Aleksandra / Fijałkowski, Wojciech / Pietrowska, Aleksandra / Matczuk, Anna Karolina / Podbielska, Halina / Wieliczko, Alina / Witkiewicz, Wojciech / Jędruchniewicz, Natalia

    International journal of molecular sciences

    2024  Volume 25, Issue 5

    Abstract: The increase in bacterial resistance to antibiotics in recent years demands innovative strategies for the detection and combating of biofilms, which are notoriously resilient. Biofilms, particularly those on contact lenses, can lead to biofilm-related ... ...

    Abstract The increase in bacterial resistance to antibiotics in recent years demands innovative strategies for the detection and combating of biofilms, which are notoriously resilient. Biofilms, particularly those on contact lenses, can lead to biofilm-related infections (e.g., conjunctivitis and keratitis), posing a significant risk to patients. Non-destructive and non-contact sensing techniques are essential in addressing this threat. Digital holographic tomography emerges as a promising solution. This allows for the 3D reconstruction of the refractive index distribution in biological samples, enabling label-free visualization and the quantitative analysis of biofilms. This tool provides insight into the dynamics of biofilm formation and maturation on the surface of transparent materials. Applying digital holographic tomography for biofilm examination has the potential to advance our ability to combat the antibiotic bacterial resistance crisis. A recent study focused on characterizing biofilm formation and maturation on six soft contact lens materials (three silicone hydrogels, three hydrogels), with a particular emphasis on
    MeSH term(s) Humans ; Biofilms ; Bacteria ; Anti-Bacterial Agents ; Hydrogels ; Contact Lenses, Hydrophilic/microbiology ; Pseudomonas aeruginosa/physiology
    Chemical Substances Anti-Bacterial Agents ; Hydrogels
    Language English
    Publishing date 2024-02-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25052653
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Avian hepatitis E virus is widespread among chickens in Poland and belongs to genotype 2

    Matczuk, Anna Karolina / Ćwiek, Katarzyna / Wieliczko, Alina

    Archives of virology. 2019 Feb., v. 164, no. 2

    2019  

    Abstract: Big liver and spleen disease, caused by avian hepatitis E virus, has been reported in Poland, but the prevalence of the virus has not yet been investigated. In this study, 1034 serum samples from 57 breeder broiler and laying hen flocks were screened for ...

    Abstract Big liver and spleen disease, caused by avian hepatitis E virus, has been reported in Poland, but the prevalence of the virus has not yet been investigated. In this study, 1034 serum samples from 57 breeder broiler and laying hen flocks were screened for the presence of anti-aHEV antibodies. In a random serology study, 56.1% of flocks were positive. Seroprevalence was higher in laying hen flocks than in broiler breeder flocks. Phylogenetic analysis of partial ORF1 and ORF2 sequences revealed that all Polish isolates belonged to genotype 2. This is the first time this genotype has been detected in Central Europe.
    Keywords Orthohepevirus B ; liver ; blood serum ; broiler breeders ; laying hens ; seroprevalence ; antibodies ; flocks ; phylogeny ; serology ; spleen ; genotype ; viruses ; Central European region ; Poland
    Language English
    Dates of publication 2019-02
    Size p. 595-599.
    Publishing place Springer Vienna
    Document type Article
    Note 2019-12-06
    ZDB-ID 7491-3
    ISSN 1432-8798 ; 0304-8608
    ISSN (online) 1432-8798
    ISSN 0304-8608
    DOI 10.1007/s00705-018-4089-y
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 70/128: Show issues

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  5. Article: Production of Recombinant EAV with Tagged Structural Protein Gp3 to Study Artervirus Minor Protein Localization in Infected Cells

    Matczuk, Anna Karolina / Chodaczek, Grzegorz / Ugorski, Maciej

    Viruses. 2019 Aug. 09, v. 11, no. 8

    2019  

    Abstract: Equine arteritis virus (EAV) is a prototype member of the Arterivirus family, comprising important pathogens of domestic animals. Minor glycoproteins of Arteriviruses are responsible for virus entry and cellular tropism. The experimental methods for ... ...

    Abstract Equine arteritis virus (EAV) is a prototype member of the Arterivirus family, comprising important pathogens of domestic animals. Minor glycoproteins of Arteriviruses are responsible for virus entry and cellular tropism. The experimental methods for studying minor Arterivirus proteins are limited because of the lack of antibodies and nested open reading frames (ORFs). In this study, we generated recombinant EAV with separated ORFs 3 and 4, and Gp3 carrying HA-tag (Gp3-HA). The recombinant viruses were stable on passaging and replicated in titers similar to the wild-type EAV. Gp3-HA was incorporated into the virion particles as monomers and as a Gp2/Gp3-HA/Gp4 trimer. Gp3-HA localized in ER and, to a lesser extent, in the Golgi, it also co-localized with the E protein but not with the N protein. The co-localization of Gp3-HA and the E protein with ERGIC was reduced. Moreover, EAV with Gp3-HA could become a valuable research tool for identifying host cell factors during infection and the role of Gp3 in virus attachment and entry.
    Keywords Equine arteritis virus ; antibodies ; domestic animals ; glycoproteins ; host-cell factors ; open reading frames ; pathogens ; prototypes ; structural proteins ; virion ; viruses ; covid19
    Language English
    Dates of publication 2019-0809
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v11080735
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Production of Recombinant EAV with Tagged Structural Protein Gp3 to Study Artervirus Minor Protein Localization in Infected Cells.

    Matczuk, Anna Karolina / Chodaczek, Grzegorz / Ugorski, Maciej

    Viruses

    2019  Volume 11, Issue 8

    Abstract: Equine arteritis virus (EAV) is a prototype member of the Arterivirus family, comprising important pathogens of domestic animals. Minor glycoproteins of Arteriviruses are responsible for virus entry and cellular tropism. The experimental methods for ... ...

    Abstract Equine arteritis virus (EAV) is a prototype member of the Arterivirus family, comprising important pathogens of domestic animals. Minor glycoproteins of Arteriviruses are responsible for virus entry and cellular tropism. The experimental methods for studying minor Arterivirus proteins are limited because of the lack of antibodies and nested open reading frames (ORFs). In this study, we generated recombinant EAV with separated ORFs 3 and 4, and Gp3 carrying HA-tag (Gp3-HA). The recombinant viruses were stable on passaging and replicated in titers similar to the wild-type EAV. Gp3-HA was incorporated into the virion particles as monomers and as a Gp2/Gp3-HA/Gp4 trimer. Gp3-HA localized in ER and, to a lesser extent, in the Golgi, it also co-localized with the E protein but not with the N protein. The co-localization of Gp3-HA and the E protein with ERGIC was reduced. Moreover, EAV with Gp3-HA could become a valuable research tool for identifying host cell factors during infection and the role of Gp3 in virus attachment and entry.
    MeSH term(s) Animals ; Arterivirus Infections/veterinary ; Cell Line ; Equartevirus/genetics ; Equartevirus/metabolism ; Genetic Engineering ; Genome, Viral ; Golgi Apparatus/metabolism ; Horse Diseases/virology ; Horses ; Host-Pathogen Interactions ; Intracellular Space ; Mutation ; Open Reading Frames ; Protein Transport ; Viral Envelope Proteins/genetics ; Viral Envelope Proteins/metabolism ; Virus Replication
    Chemical Substances GP3 protein, equine arteritis virus ; Viral Envelope Proteins
    Keywords covid19
    Language English
    Publishing date 2019-08-09
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v11080735
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Book ; Online ; Thesis: Membrane topology and processing of the minor glycoproteins of equine arteritis virus

    Matczuk, Anna Karolina

    2014  

    Title variant Membrantopologie und Prozessierung der Glykoproteine Gp2/3/4 des Equinen Arteritis Virus
    Author's details vorgelegt von Anna Karolina Matczuk
    Language English
    Size Online-Ressource, Ill., graph. Darst.
    Publisher Mensch und Buch Verl
    Publishing place Berlin
    Document type Book ; Online ; Thesis
    Thesis / German Habilitation thesis Freie Univ., Diss.--Berlin, 2014
    ISBN 9783863874735 ; 3863874730
    Database Special collection on veterinary medicine and general parasitology

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  8. Book ; Online ; Thesis: Membrane topology and processing of the minor glycoproteins of equine arteritis virus

    Matczuk, Anna Karolina

    2014  

    Title variant Membrantopologie und Prozessierung der Glykoproteine Gp2/3/4 des Equinen Arteritis Virus
    Author's details vorgelegt von Anna Karolina Matczuk
    Language English
    Size Online-Ressource, Ill., graph. Darst.
    Publisher Mensch und Buch Verl
    Publishing place Berlin
    Document type Book ; Online ; Thesis
    Thesis / German Habilitation thesis Freie Univ., Diss.--Berlin, 2014
    ISBN 9783863874735 ; 3863874730
    Database Former special subject collection: coastal and deep sea fishing

    Full text online

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    Inter-library loan at ZB MED

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  9. Book ; Thesis: Membrane topology and processing of the minor glycoproteins of equine arteritis virus

    Matczuk, Anna Karolina

    2014  

    Author's details vorgelegt von Anna Karolina Matczuk
    Keywords Glykoproteine ; Membranproteine ; Equines Arteriitis-Virus ; Signaltransduktion
    Language English
    Size 109 S., Ill., graph. Darst.
    Publisher Mensch & Buch
    Publishing place Berlin
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Freie Univ., Diss.--Berlin, 2014
    ISBN 9783863874735 ; 3863874730
    Database Special collection on veterinary medicine and general parasitology

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    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  10. Article ; Online: Avian hepatitis E virus is widespread among chickens in Poland and belongs to genotype 2.

    Matczuk, Anna Karolina / Ćwiek, Katarzyna / Wieliczko, Alina

    Archives of virology

    2018  Volume 164, Issue 2, Page(s) 595–599

    Abstract: Big liver and spleen disease, caused by avian hepatitis E virus, has been reported in Poland, but the prevalence of the virus has not yet been investigated. In this study, 1034 serum samples from 57 breeder broiler and laying hen flocks were screened for ...

    Abstract Big liver and spleen disease, caused by avian hepatitis E virus, has been reported in Poland, but the prevalence of the virus has not yet been investigated. In this study, 1034 serum samples from 57 breeder broiler and laying hen flocks were screened for the presence of anti-aHEV antibodies. In a random serology study, 56.1% of flocks were positive. Seroprevalence was higher in laying hen flocks than in broiler breeder flocks. Phylogenetic analysis of partial ORF1 and ORF2 sequences revealed that all Polish isolates belonged to genotype 2. This is the first time this genotype has been detected in Central Europe.
    MeSH term(s) Animals ; Antibodies, Viral/blood ; Chickens ; Female ; Genotype ; Hepatitis, Viral, Animal/blood ; Hepatitis, Viral, Animal/epidemiology ; Hepatitis, Viral, Animal/virology ; Hepevirus/classification ; Hepevirus/genetics ; Hepevirus/immunology ; Hepevirus/isolation & purification ; Male ; Phylogeny ; Poland/epidemiology ; Poultry Diseases/blood ; Poultry Diseases/epidemiology ; Poultry Diseases/virology ; Seroepidemiologic Studies
    Chemical Substances Antibodies, Viral
    Language English
    Publishing date 2018-11-03
    Publishing country Austria
    Document type Journal Article
    ZDB-ID 7491-3
    ISSN 1432-8798 ; 0304-8608
    ISSN (online) 1432-8798
    ISSN 0304-8608
    DOI 10.1007/s00705-018-4089-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.110: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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