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  1. Article ; Online: Como colocar em prática o Plano de Atendimento às Emergências (PAE) no laboratório clínico How to implement the Plan of Care for Emergencies (LAP) in the clinical laboratory

    Maria Elizabete Mendes / Maria Leide Badaró / Evelyn Rodrigues / Maurílio Pacheco-Neto / Nairo Massakazu Sumita

    Jornal Brasileiro de Patologia e Medicina Laboratorial, Vol 47, Iss 3, Pp 225-

    2011  Volume 231

    Abstract: INTRODUÇÃO: A situação socioeconômica e ambiental na qual os laboratórios clínicos vivem, aliada aos riscos ampliados do negócio nos dias atuais, exige dos dirigentes a elaboração de um plano de segurança para situações de catástrofes. OBJETIVO: Esse ... ...

    Abstract INTRODUÇÃO: A situação socioeconômica e ambiental na qual os laboratórios clínicos vivem, aliada aos riscos ampliados do negócio nos dias atuais, exige dos dirigentes a elaboração de um plano de segurança para situações de catástrofes. OBJETIVO: Esse plano é útil para garantir a continuidade do negócio e sua recuperação após a crise. MÉTODO: O Plano de Atendimento à Emergência (PAE) é apresentado como um conjunto de procedimentos estruturados para a obtenção de respostas rápidas, adestradas e eficientes em situações de emergência no laboratório. CONCLUSÃO: Ele visa prevenir ou mitigar as eventuais consequências adversas para segurança, saúde e meio ambiente no âmbito laboratorial. RESULTADO: Este artigo discute a aplicabilidade, as responsabilidades, a elaboração e a manutenção, assim como suas implicações na rotina laboratorial INTRODUCTION: Nowadays, the environmental and socioeconomic contexts in which clinical laboratories are set, coupled with increased business risks, require the formulation of an emergency care plan in case of natural disasters. OBJECTIVE: This plan is useful to ensure business continuity and recovery after crisis. METHOD: The Emergency Care Plan (PAE) is presented as a structured set of procedures for obtaining rapid, efficient and trained responses in emergency situations. CONCLUSION: It aims at preventing or mitigating occasional adverse consequences regarding safety, health and environment in clinical laboratories. RESULTS: This article discusses the applicability, responsibilities, development and maintenance as well as their corresponding implications in laboratory procedures
    Keywords Laboratório clínico ; Meio ambiente ; Gestão de riscos ; Catástrofes ; Emergências ; Gestão de crises ; Clinical laboratory ; Environment ; Risk management ; Disasters ; Emergencies ; Crisis management ; Pathology ; RB1-214 ; Medicine ; R ; DOAJ:Pathology ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
    Language English
    Publishing date 2011-06-01T00:00:00Z
    Publisher Sociedade Brasileira de Patologia Clínica
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Practical aspects of the use of FMEA tool in clinical laboratory risk management

    Maria Elizabete Mendes / Pérsio de Almeida Rezende Ebner / Paschoalina Romano / Maurílio Pacheco Neto / Alexandre Sant’anna / Nairo Massakazu Sumita

    Jornal Brasileiro de Patologia e Medicina Laboratorial, Vol 49, Iss 3, Pp 174-

    2013  Volume 181

    Abstract: INTRODUCTION: This paper presents the failure modes and effects analysis (FMEA) tool in a clinical laboratory through the introduction of new technology for blood gas and serum ionized calcium in multi-parameter analyzers such as Point of Care Testing ( ... ...

    Abstract INTRODUCTION: This paper presents the failure modes and effects analysis (FMEA) tool in a clinical laboratory through the introduction of new technology for blood gas and serum ionized calcium in multi-parameter analyzers such as Point of Care Testing (POCT). OBJECTIVE: To present FMEA as a tool for risk managing and improvement with the introduction of new technologies in a public laboratory. METHODS: The change of multiparameter gas analyzer type POCT was defined and described as a process. Subsequently, the criteria were presented to the risk assessment and its quantification. We studied the failure modes that might occur in this process. We established three action plans involving improvements to be made in the technological change. FMEA was applied in two stages: at the beginning of the project and after the implementation of the proposed measures. RESULTS: The first plan involved administrative measures related to the bidding process; the second preventive action involved the possibility of which supplier would win the bid by studying the efficiency of the analyzer and its impact on productivity; the third set of actions was directed to improvements in the relationship with the clinical staff in order to minimize occasional complaints. The last actions referred to employing new employees to meet the growing demand. CONCLUSION: FMEA proved to be a reliable tool for performance improvement, which proactively identifies, prioritizes and mitigates patient risks.
    Keywords laboratório clínico ; gestão de riscos ; análise de riscos ; FMEA ; segurança do paciente ; gasometria ; Pathology ; RB1-214 ; Medicine ; R
    Subject code 690
    Language English
    Publishing date 2013-06-01T00:00:00Z
    Publisher Sociedade Brasileira de Patologia Clínica
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Effects of long-term administration of omeprazole on bone mineral density and the mechanical properties of the bone

    Gabriela Rezende Yanagihara / Aline Goulart de Paiva / Maurílio Pacheco Neto / Larissa Helena Torres / Antônio Carlos Shimano / Mário Jefferson Quirino Louzada / Raquel Annoni / Álvaro César de Oliveira Penoni

    Revista Brasileira de Ortopedia, Vol 50, Iss 2, Pp 232-

    2015  Volume 238

    Abstract: OBJECTIVES: Epidemiological studies have shown a relationship between long-term use of proton pump inhibitors and bone metabolism. However, this relationship has not yet become established. The aim of the present study was to analyze the mechanical ... ...

    Abstract OBJECTIVES: Epidemiological studies have shown a relationship between long-term use of proton pump inhibitors and bone metabolism. However, this relationship has not yet become established. The aim of the present study was to analyze the mechanical properties and bone mineral density (BMD) of rats that were subjected to long-term omeprazole use.METHODS: Fifty Wistar rats weighing between 200 and 240 g were divided equally into five groups: OMP300 (omeprazole intake at a dose of 300 µmoL/kg/day); OMP200 (200 µmoL/kg/day); OMP40 (40 µmoL/kg/day); OMP10 (10 µmoL/kg/day); and Cont (control group; intake of dilution vehicle). The solutions were administered for 90 consecutive days. After the rats had been sacrificed, their BMD, the mechanical properties of the dissected femurs and their serum Ca++ levels were analyzed.RESULTS: The BMD of the OMP300 group was lower than that of the controls (p = 0.006). There was no difference on comparing the OMP200, OMP40 and OMP10 groups with the controls. The maximum strength and rigidity of the femur did not differ in the experimental groups in comparison with the controls. The OMP300 group had a statistically lower serum Ca++ concentration than that of the controls (p = 0.049), but the other groups did not show any difference in relation to the controls.CONCLUSION: Daily intake of 300 µmoL/kg/day of omeprazole decreased the BMD of the femur, but without changes to the rigidity and strength of the femur in adult rats.
    Keywords Osso ; Densidade óssea ; Omeprazol ; Ratos ; Medicine ; R ; Orthopedic surgery ; RD701-811
    Subject code 630
    Language English
    Publishing date 2015-04-01T00:00:00Z
    Publisher Sociedade Brasileira de Ortopedia e Traumatologia
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Exposure of Neonatal Mice to Tobacco Smoke Disturbs Synaptic Proteins and Spatial Learning and Memory from Late Infancy to Early Adulthood.

    Larissa Helena Torres / Raphael C T Garcia / Anne M M Blois / Lívia M M Dati / Ana Carolina Durão / Adilson Silva Alves / Maurílio Pacheco-Neto / Thais Mauad / Luiz R G Britto / Gilberto Fernando Xavier / Rosana Camarini / Tania Marcourakis

    PLoS ONE, Vol 10, Iss 8, p e

    2015  Volume 0136399

    Abstract: Exposure to environmental tobacco smoke (ETS) in the early postnatal period has been associated with several diseases; however, little is known about the brain effects of ETS exposure during this critical developmental period or the long-term ... ...

    Abstract Exposure to environmental tobacco smoke (ETS) in the early postnatal period has been associated with several diseases; however, little is known about the brain effects of ETS exposure during this critical developmental period or the long-term consequences of this exposure. This study investigated the effects of the early postnatal ETS exposure on both reference and working memory, synaptic proteins and BDNF from late infancy to early adulthood (P3-P73). BALB/c mice were exposed to ETS generated from 3R4F reference research cigarettes (0.73 mg of nicotine/cigarette) from P3 to P14. Spatial reference and working memory were evaluated in the Morris water maze during infancy (P20-P29), adolescence (P37-P42) and adulthood (P67-P72). Synapsin, synaptophysin, PSD95 and brain-derived neurotrophic factor (BDNF) were assessed at P15, P35 and P65 by immunohistochemistry and immunoblotting. Mice that were exposed to ETS during the early postnatal period showed poorer performance in the spatial reference memory task. Specifically, the ETS-exposed mice exhibited a significantly reduced time and distance traveled in the target quadrant and in the platform location area than the controls at all ages evaluated. In the spatial working memory task, ETS disrupted the maintenance but not the acquisition of the critical spatial information in both infancy and adolescence. ETS also induced changes in synaptic components, including decreases in synapsin, synaptophysin, PSD95 and BDNF levels in the hippocampus. Exposure to ETS in the early postnatal period disrupts both spatial reference and working memory; these results may be related to changes in synaptogenesis in the hippocampus. Importantly, most of these effects were not reversed even after a long exposure-free period.
    Keywords Medicine ; R ; Science ; Q
    Subject code 333
    Language English
    Publishing date 2015-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Improvement of an HPLC method to determine urinary δ-aminolevulinic acid Aprimoramento de um método de HPLC para determinar ácido δ-aminolevulínico urinário

    Atecla Nunciata Lopes Alves / Nairo Massakazu Sumita / Alexandre Soriano Fortini / Maurilio Pacheco Neto / Maria Elizabete Mendes / Alberto José da Silva Duarte

    Jornal Brasileiro de Patologia e Medicina Laboratorial, Vol 46, Iss 3, Pp 171-

    2010  Volume 174

    Abstract: OBJECTIVE: The purpose of this study was to evaluate and improve a high performance liquid chromatography (HPLC) methodology to determine urinary δ-aminolevulinic acid (ALA-U) with small volumes of sample. METHOD: The method was based on the formation of ...

    Abstract OBJECTIVE: The purpose of this study was to evaluate and improve a high performance liquid chromatography (HPLC) methodology to determine urinary δ-aminolevulinic acid (ALA-U) with small volumes of sample. METHOD: The method was based on the formation of a fluorescent compound and subsequent 15-minute chromatographic run. RESULTS: The method shows suitable linearity, precision and recovery. Urine samples showed 1.2 ± 0.9 mg/l (media ± standard deviation) of ALA-U. CONCLUSION: The method was considered suitable for the routine analysis of ALA-U. INTRODUÇÃO E OBJETIVO: A proposta deste estudo foi avaliar e aprimorar uma metodologia de cromatografia líquida de alta eficiência (CLAE)(11, 12), a fim de determinar o ácido δ-aminolevulínico urinário (ALA-U) utilizando volumes reduzidos de amostra. MÉTODO: O método baseia-se na formação de um composto fluorescente e posterior corrida cromatográfica de 15 minutos. RESULTADOS: O método apresentou linearidade, precisão e recuperação adequadas. Os resultados para as amostras de urina testadas foram 1,2 ± 0,9 mg/l (média ± desvio padrão) de ALA-U. CONCLUSÃO: O método foi considerado adequado para análises de rotina de ALA-U.
    Keywords Ácido δ-aminolevulínico urinário ; Cromatografia líquida de alta eficiência ; Porfirias agudas ; Urinary δ-aminolevulinic acid ; High performance liquid chromatography ; Acute porphyrias ; Pathology ; RB1-214 ; Medicine ; R ; DOAJ:Pathology ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
    Language English
    Publishing date 2010-06-01T00:00:00Z
    Publisher Sociedade Brasileira de Patologia Clínica
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Monitoração terapêutica da azatioprina

    Maurílio Pacheco Neto / Atecla Nunciata Lopes Alves / Alexandre Soriano Fortini / Marcelo do Nascimento Burattini / Nairo Massakazu Sumita / Miguel Srougi / Pedro Renato Chocair

    Jornal Brasileiro de Patologia e Medicina Laboratorial, Vol 44, Iss 3, Pp 161-

    uma revisão Therapeutic drug monitoring of azathioprine: a review

    2008  Volume 167

    Abstract: Os nucleotídeos de tioguanina (6-TGN), metabólitos ativos da azatioprina (AZA) e da 6-mercaptopurina (6-MP), atuam como antagonistas das purinas, inibindo as sínteses de DNA, RNA e a protéica, e induzindo à citotoxicidade/imunossupressão. A enzima ... ...

    Abstract Os nucleotídeos de tioguanina (6-TGN), metabólitos ativos da azatioprina (AZA) e da 6-mercaptopurina (6-MP), atuam como antagonistas das purinas, inibindo as sínteses de DNA, RNA e a protéica, e induzindo à citotoxicidade/imunossupressão. A enzima geneticamente determinada, tiopurina metiltransferase (TPMT), está envolvida no metabolismo desses agentes e, hipoteticamente, determina a resposta clínica às tiopurinas. A baixa atividade dessa enzima diminui a metilação das tiopurinas, resultando em potencial sobredose, enquanto altos níveis de TPMT levam à superprodução do metabólito tóxico 6-metilmercaptopurina (6-MMP) e à não-efetividade terapêutica da AZA e da 6-MP. Várias mutações no gene da TPMT têm sido identificadas e correlacionadas com fenótipos de baixa atividade. Neste artigo, também se discute a monitoração terapêutica desses fármacos por meio da medida dos níveis de 6-TGN intra-eritrocitários, os quais se correlacionam com imunossupressão e mielotoxicidade. Já a 6-MMP está diretamente relacionada com hepatotoxicidade. Esses ensaios estão associados ao uso de doses adequadas dessa droga, resultando num melhor controle da doença e menor uso de corticosteróides. Thioguanine nucleotides (6-TGN), active metabolites of azathioprine (AZA) and 6-mercaptopurine (6-MP), act as purine antagonists, inhibiting DNA, RNA, and protein synthesis and inducing cytotoxicity and immunosuppression. The genetically determined thiopurine methyltransferase enzyme (TPMT) is involved in the metabolism of these agents and, theoretically, determines the clinical response to thiopurines. Low activity of this enzyme decreases the methylation of thiopurines, what results in potential overdosing, whereas high TPMT status leads to overproduction of toxic metabolite 6-methilmercaptopurine (6-MMP) and ineffectiveness of AZA and 6-MP. Several mutations in the TPMT gene have been identified and correlated with low activity phenotypes. In this study, we also discuss the therapeutic monitoring of these drugs by means of red blood cell 6-TGN levels, which correlate with immunosuppression and mielotoxicity. 6-MMP is directly connected with hepatotoxicity. These metabolites assays are associated with the use of appropriate doses of this drug, what results in a better control of the disease and a decreased use of corticosteroids.
    Keywords Azatioprina ; 6-mercaptopurina ; 6-tioguanina ; Imunossupressão ; Cromatografia líquida ; Monitorização de medicamentos ; 6-mercaptopurine ; 6-thioguanine ; Azathioprine ; Drug monitoring ; Immunosuppression ; Liquid chromatography ; Pathology ; RB1-214 ; Medicine ; R ; DOAJ:Pathology ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
    Language English
    Publishing date 2008-06-01T00:00:00Z
    Publisher Sociedade Brasileira de Patologia Clínica
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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