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  1. Book: T-Cell trafficking

    Rainger, George Edward / Mcgettrick, Helen M.

    methods and protocols

    (Methods in molecular biology ; 1591 ; Springer protocols)

    2017  

    Series title Methods in molecular biology ; 1591
    Springer protocols
    Collection
    Keywords T-Cell ; antigens ; immune response ; immune system ; lymphocytes
    Subject code 610
    Language English
    Size xi, 252 Seiten, Illustrationen, 25.4 cm x 17.8 cm, 0 g
    Edition Second edition
    Publisher Humana Press
    Publishing place New York, NY
    Publishing country United States
    Document type Book
    HBZ-ID HT019305837
    ISBN 978-1-4939-6929-6 ; 1-4939-6929-3 ; 9781493969319 ; 1493969315
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 7042 -1591- verfügbar in Königswinter Königswinter

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  2. Article ; Online: Introduction: T Cell Trafficking in Inflammation and Immunity.

    Chimen, Myriam / Apta, Bonita H R / Mcgettrick, Helen M

    Methods in molecular biology (Clifton, N.J.)

    2016  Volume 1591, Page(s) 73–84

    Abstract: T cell migration across vascular endothelium is essential for T cell responses, as through the expression of specific tissue-homing receptors, these cells then access peripheral tissues, with the goal of eliminating invading pathogens and/or tumor cells. ...

    Abstract T cell migration across vascular endothelium is essential for T cell responses, as through the expression of specific tissue-homing receptors, these cells then access peripheral tissues, with the goal of eliminating invading pathogens and/or tumor cells. However, aberrant trafficking of T cells to peripheral tissues contributes to the development of most chronic inflammatory diseases. Very little is known about the mechanisms by which T cell trafficking is regulated during inflammation, and it is thus difficult to target this aspect of pathology for the development of new therapies. It is therefore important to understand the pathways involved in regulating the recruitment of immune cells.
    MeSH term(s) Animals ; Cell Movement/immunology ; Endothelium, Vascular/immunology ; Humans ; Immunity/immunology ; Inflammation/immunology ; T-Lymphocytes/immunology ; T-Lymphocytes/physiology
    Language English
    Publishing date 2016-06-24
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-6931-9_6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Endocrine Regulation of Lymphocyte Trafficking In Vitro.

    Apta, Bonita H R / Chimen, Myriam / Mcgettrick, Helen M

    Methods in molecular biology (Clifton, N.J.)

    2016  Volume 1591, Page(s) 101–119

    Abstract: Lymphocyte recruitment in inflammation can be influenced by many molecules including cytokines, chemokines, and adipokines. In our lab, we have examined the effects of the adipokines leptin and adiponectin on lymphocyte migration, and observed modulation ...

    Abstract Lymphocyte recruitment in inflammation can be influenced by many molecules including cytokines, chemokines, and adipokines. In our lab, we have examined the effects of the adipokines leptin and adiponectin on lymphocyte migration, and observed modulation of this process. Lymphocyte behavior can be assessed in the lab under static conditions, or can be studied under flow, simulating in vivo conditions. In this chapter, in vitro methods for analyzing adhesion and migration of lymphocytes isolated from blood are described in detail. In static adhesion and migration assays, lymphocytes are allowed to settle on top of endothelial cell monolayers cultured in plates for a desired period of time. In the flow-based assay, lymphocytes are perfused over the endothelium at a continuous rate through microchannels which are commercially available. Depending on the choice of method employed, the efficiency of lymphocytes to adhere to and migrate across the endothelial cell monolayer under different conditions can be evaluated. Static assays are less complex and are of higher throughput. However, these assays provide less detailed information regarding lymphocyte behaviors. On the other hand, the flow-based assays are more difficult to perform, but are more physiologically relevant due to the presence of flow and yield more detailed information about lymphocyte activities such as capture, immobilization, and migration in real-time.
    MeSH term(s) Cell Adhesion/physiology ; Cell Movement/physiology ; Cells, Cultured ; Chemokines/metabolism ; Endocrine System/metabolism ; Endocrine System/physiology ; Endothelial Cells/metabolism ; Endothelial Cells/physiology ; Endothelium, Vascular/metabolism ; Endothelium, Vascular/physiology ; Humans ; Lymphocytes/metabolism ; Lymphocytes/physiology
    Chemical Substances Chemokines
    Language English
    Publishing date 2016-06-24
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-6931-9_8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Mesenchymal Stromal Cells as Active Regulators of Lymphocyte Recruitment to Blood Vascular Endothelial Cells.

    Mcgettrick, Helen M / Ward, Lewis S C / Rainger, George Edward / Nash, Gerard B

    Methods in molecular biology (Clifton, N.J.)

    2017  Volume 1591, Page(s) 121–142

    Abstract: Methods are described for analyzing adhesion and migration of isolated lymphocytes on endothelial cell monolayers which have been cocultured with different mesenchymal stromal cells, with or without additional cytokine treatment. The different cells ... ...

    Abstract Methods are described for analyzing adhesion and migration of isolated lymphocytes on endothelial cell monolayers which have been cocultured with different mesenchymal stromal cells, with or without additional cytokine treatment. The different cells types are grown on opposite sides of 3.0 or 0.4 μm pore filters, depending on whether migration through the whole construct is to be analyzed, or adhesion to the endothelial cells alone. Migration away from the sub-endothelial space and through the stromal layer can also be assessed by culturing mesenchymal stromal cells within a 3-D collagen gel overlaid with endothelial cells. Assays may be "static" or the filter-based constructs can be incorporated into flow chambers so that cell behavior can be directly observed under conditions simulating those in vivo. In general, by choice of method, one can evaluate efficiency of attachment, and ability of cells to migrate across the endothelial monolayer, through the filter and through the stromal cell layer in 2-D or 3-D. Fluorescence microscopic examination of fixed filters can be used, e.g., to ascertain whether lymphocytes are retained by stromal cells. In general, static assays have the higher throughput and greatest ease of use, while the flow-based assays are more physiologically relevant and allow detailed recording of cell behavior in real time.
    MeSH term(s) Cell Adhesion/physiology ; Cell Movement/physiology ; Cells, Cultured ; Coculture Techniques ; Collagen/metabolism ; Endothelial Cells/metabolism ; Endothelial Cells/physiology ; Endothelium, Vascular/metabolism ; Endothelium, Vascular/physiology ; Fibroblasts/metabolism ; Fibroblasts/physiology ; Humans ; Lymphocytes/metabolism ; Lymphocytes/physiology ; Mesenchymal Stem Cells/metabolism ; Mesenchymal Stem Cells/physiology
    Chemical Substances Collagen (9007-34-5)
    Language English
    Publishing date 2017-03-27
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-6931-9_9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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