Article ; Online: Very rapid cloning, expression and identifying specificity of T-cell receptors for T-cell engineering.
PloS one
2020 Volume 15, Issue 2, Page(s) e0228112
Abstract: Neoantigens can be predicted and in some cases identified using the data obtained from the whole exome sequencing and transcriptome sequencing of tumor cells. These sequencing data can be coupled with single-cell RNA sequencing for the direct ... ...
| Abstract | Neoantigens can be predicted and in some cases identified using the data obtained from the whole exome sequencing and transcriptome sequencing of tumor cells. These sequencing data can be coupled with single-cell RNA sequencing for the direct interrogation of the transcriptome, surfaceome, and pairing of αβ T-cell receptors (TCRαβ) from hundreds of single T cells. Using these 2 large datasets, we established a platform for identifying antigens recognized by TCRαβs obtained from single T cells. Our approach is based on the rapid expression of cloned TCRαβ genes as Sleeping Beauty transposons and the determination of the introduced TCRαβs' antigen specificity and avidity using a reporter cell line. The platform enables the very rapid identification of tumor-reactive TCRs for the bioengineering of T cells with redirected specificity. |
|---|---|
| MeSH term(s) | Cell Engineering/methods ; Cloning, Molecular/methods ; Gene Expression ; Gene Library ; Genes, MHC Class I/genetics ; Genes, MHC Class II/genetics ; HEK293 Cells ; Humans ; Kinetics ; Receptors, Antigen, T-Cell/genetics ; Receptors, Antigen, T-Cell, alpha-beta/genetics ; T-Lymphocytes/cytology ; T-Lymphocytes/metabolism |
| Chemical Substances | Receptors, Antigen, T-Cell ; Receptors, Antigen, T-Cell, alpha-beta |
| Language | English |
| Publishing date | 2020-02-10 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
| ISSN | 1932-6203 |
| ISSN (online) | 1932-6203 |
| DOI | 10.1371/journal.pone.0228112 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
Inter-library loan at ZB MED
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.