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  1. Article ; Online: A CROSS-SECTIONAL STUDY OF STRESS AMONG UNDERGRADUATE MEDICAL STUDENTS IN A TERTIARY CARE TEACHING INSTITUTE, JHARKHAND.

    Md Shadab Alam / Dr. Rajendra Kumar / Lakhan Majhee

    Student's Journal of Health Research Africa, Vol 4, Iss

    2023  Volume 12

    Abstract: Background Medical students are more exposed to stressful situations due to their academic pressure, difficult learning environment, and challenging competency-based medical education design that does not provide enough time for their personal life ... ...

    Abstract Background Medical students are more exposed to stressful situations due to their academic pressure, difficult learning environment, and challenging competency-based medical education design that does not provide enough time for their personal life events. So, chronic stress among medical students results in depression, substance abuse, and even suicide. This study aims to determine the prevalence of stress among undergraduate medical students of RIMS, Ranchi, Jharkhand, India. Methodology This was a cross-sectional study conducted among 258 undergraduate medical students of RIMS, Ranchi from January 2022 to December 2022. Perceived Stress Scale-10 was used to evaluate the degree of stress among undergraduate medical students. Data obtained was analyzed using MS Excel and SSS based on SPSS and Minitab (2018). Results A total of 258 undergraduate medical students participated in the study of which 41.4% were male and 58.52% were female. Although a moderate stress rate of 68.9% was registered in most participants, 22.48% were affected by high stress. Participants in the 4th professional MBBS are more likely to experience high stress (45.06%) as compared to students in the 2nd professional MBBS, 1st professional MBBS, and 3rd professional MBBS respectively. The difference in stress severity was statistically significant at p <0.05. Conclusion Most undergraduate medical students (68.99%) have moderate stress. Female (29.8%) are more likely to have high stress. The final professional MBBS students (44.06%) have more high stress. Recommendation Counseling services to medical college students are strongly recommended to address the stress.
    Keywords Stress ; Undergraduate-Medical Students ; Cross-Sectional Study ; Prevalence ; General works ; R5-130.5 ; Infectious and parasitic diseases ; RC109-216 ; Surgery ; RD1-811 ; Public aspects of medicine ; RA1-1270
    Language English
    Publishing date 2023-12-01T00:00:00Z
    Publisher Student's Journal of Health Research
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: RGD engineered dendrimer nanotherapeutic as an emerging targeted approach in cancer therapy

    Sheikh, Afsana / Md, Shadab / Kesharwani, Prashant

    Journal of controlled release. 2021 Dec. 10, v. 340

    2021  

    Abstract: A bird's eye view is now demanded in the area of cancer research to suppress the suffering of cancer patient and mediate the lack of treatment related to chemotherapy. Chemotherapy is always preferred over surgery or radiation therapy, but they never met ...

    Abstract A bird's eye view is now demanded in the area of cancer research to suppress the suffering of cancer patient and mediate the lack of treatment related to chemotherapy. Chemotherapy is always preferred over surgery or radiation therapy, but they never met the patient's demand of safe medication. Targeted therapy has now been in research that could hinder the unnecessary effect of drug on normal cells but could affect the tumor cells in much efficient manner. Angiogenesis is process involved in development of new blood vessel that nourishes tumor growth. Integrin receptors are over expressed on cancer cells that play vital role in angiogenesis for growth and metastasis of tumor cell. A delivery of RGD based peptide to integrin targeted site could help in its successful binding and liberation of drug in tumor vasculature. Dendrimers, in addition to its excellent pharmacokinetic properties also helps to carry targeting ligand to site of tumor by successfully conjugating with them. The aim of this review is to bring light upon the role of integrin in cancer progression, interaction of RGD to integrin receptor and more importantly the RGD-dendrimer based targeted therapy for the treatment of various cancers.
    Keywords angiogenesis ; blood vessels ; cancer therapy ; dendrimers ; drug therapy ; drugs ; integrins ; ligands ; metastasis ; neoplasm cells ; neoplasm progression ; neoplasms ; patients ; peptides ; pharmacokinetics ; radiotherapy ; surgery
    Language English
    Dates of publication 2021-1210
    Size p. 221-242.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 632533-6
    ISSN 1873-4995 ; 0168-3659
    ISSN (online) 1873-4995
    ISSN 0168-3659
    DOI 10.1016/j.jconrel.2021.10.028
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  3. Article: Recent Progress of RGD Modified Liposomes as Multistage Rocket Against Cancer.

    Sheikh, Afsana / Alhakamy, Nabil A / Md, Shadab / Kesharwani, Prashant

    Frontiers in pharmacology

    2022  Volume 12, Page(s) 803304

    Abstract: Cancer is a life-threatening disease, contributing approximately 9.4 million deaths worldwide. To address this challenge, scientific researchers have investigated molecules that could act as speed-breakers for cancer. As an abiotic drug delivery system, ... ...

    Abstract Cancer is a life-threatening disease, contributing approximately 9.4 million deaths worldwide. To address this challenge, scientific researchers have investigated molecules that could act as speed-breakers for cancer. As an abiotic drug delivery system, liposomes can hold both hydrophilic and lipophilic drugs, which promote a controlled release, accumulate in the tumor microenvironment, and achieve elongated half-life with an enhanced safety profile. To further improve the safety and impair the off-target effect, the surface of liposomes could be modified in a way that is easily identified by cancer cells, promotes uptake, and facilitates angiogenesis. Integrins are overexpressed on cancer cells, which upon activation promote downstream cell signaling and eventually activate specific pathways, promoting cell growth, proliferation, and migration. RGD peptides are easily recognized by integrin over expressed cells. Just like a multistage rocket, ligand anchored liposomes can be selectively recognized by target cells, accumulate at the specific site, and finally, release the drug in a specific and desired way. This review highlights the role of integrin in cancer development, so gain more insights into the phenomenon of tumor initiation and survival. Since RGD is recognized by the integrin family, the fate of RGD has been demonstrated after its binding with the acceptor's family. The role of RGD based liposomes in targeting various cancer cells is also highlighted in the paper.
    Language English
    Publishing date 2022-01-25
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2021.803304
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  4. Article: The Pattern of Disposal Practices of Unused and Expired Medications Among Healthcare Professionals: A Cross-Sectional Survey in Rajendra Institute of Medical Sciences, Ranchi, Jharkhand.

    Kumari, Abha / Alam, Md Shadab / Kujur, Manisha / Kumar, Sandeep

    Cureus

    2022  Volume 14, Issue 8, Page(s) e27555

    Abstract: Background: Medicines play a crucial role in treating various diseases. Most individuals, in their haste to get through their day, have developed the bad habit of improperly discarding their unwanted or expired prescriptions, which poses a number of ... ...

    Abstract Background: Medicines play a crucial role in treating various diseases. Most individuals, in their haste to get through their day, have developed the bad habit of improperly discarding their unwanted or expired prescriptions, which poses a number of risks to human health and contributes to environmental degradation. Disposal procedures of unused and expired pharmaceuticals among health care personnel at tertiary care centers have been the subject of very little research to date. Therefore, the purpose of this study is to examine how medical staff at Rajendra Institute of Medical Sciences in Ranchi, Jharkhand, handle stale or unwanted prescription drugs.
    Methods: The design of the study was cross-sectional analytical conducted among 385 health care professionals. Pretested semi-structured questionnaires prepared in Google Forms (Google LLC, Mountain View, California, United States) have been used for evaluation.
    Results: The completely filled questionnaires were analyzed, evaluated, and expressed in the percentage using Microsoft Excel (Microsoft Corporation, Redmond, Washington, United States). The most common method of disposal of unused and expired medications was throwing them in the dustbin.
    Conclusions: In this study, although, all the participants were health care professionals, because of the hectic schedule majority of them used unsafe methods of drug disposal. So, in order to protect our ecosystem, appropriate policy should be made to implement the methods for the safe disposal of unused and expired medications.
    Language English
    Publishing date 2022-08-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2747273-5
    ISSN 2168-8184
    ISSN 2168-8184
    DOI 10.7759/cureus.27555
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  5. Article: Repurposing Itraconazole Loaded PLGA Nanoparticles for Improved Antitumor Efficacy in Non-Small Cell Lung Cancers.

    Alhakamy, Nabil A / Md, Shadab

    Pharmaceutics

    2019  Volume 11, Issue 12

    Abstract: Itraconazole (ITR) is a broad-spectrum antifungal drug, which has been shown to possess some promising anticancer, anti-proliferative, and anti-angiogenic properties in some cancers, such as cancers of the lung, breast, and skin. However, ITR has some ... ...

    Abstract Itraconazole (ITR) is a broad-spectrum antifungal drug, which has been shown to possess some promising anticancer, anti-proliferative, and anti-angiogenic properties in some cancers, such as cancers of the lung, breast, and skin. However, ITR has some drawbacks, such as poor water solubility, which hinder its use as a therapeutic agent. Therefore, in the present study, we developed and characterized chitosan-coated PLGA nanoparticles of itraconazole and studied their anticancer activities in H1299 lung cancer cells. The prepared ITR nanoparticles showed a small particle size, narrow poly dispersity index (PDI), positive zeta potential, and a controlled drug release profile. The cytotoxicity of ITR nanoparticles (NPs) on H1299 cancer cells after 24 h of exposure was greater than that of the ITR solution. Apoptosis of cancer cells exposed to ITR nanoparticles was also enhanced in comparison with the ITR solution. At the molecular level, ITR NPs were more effective than ITR solution in inducing pro-apoptotic Bax and p53 while reducing anti-apoptotic Bcl2 protein expression. ITR NPs were more effective than ITR solution in arresting cells both at the G0/G1 as well as G2/M phases of the cell cycle. Hence, repurposing itraconazole by encapsulation into PLGA NPs with chitosan coating is a potentially promising approach to treat lung cancers.
    Language English
    Publishing date 2019-12-16
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527217-2
    ISSN 1999-4923
    ISSN 1999-4923
    DOI 10.3390/pharmaceutics11120685
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  6. Article ; Online: Smart Oral pH-Responsive Dual Layer Nano-Hydrogel for Dissolution Enhancement and Targeted Delivery of Naringenin Using Protein-Polysaccharides Complexation Against Colorectal Cancer.

    Md, Shadab / Abdullah, Samaa / Awan, Zuhier A / Alhakamy, Nabil A

    Journal of pharmaceutical sciences

    2022  Volume 111, Issue 11, Page(s) 3155–3164

    Abstract: Naringenin (NAR) is a natural anticancer, but it has not been developed for clinical use despite its therapeutic potential due to its low water solubility, low membrane permeability, first-pass metabolism, and low bioavailability. To overcome these ... ...

    Abstract Naringenin (NAR) is a natural anticancer, but it has not been developed for clinical use despite its therapeutic potential due to its low water solubility, low membrane permeability, first-pass metabolism, and low bioavailability. To overcome these problems, the optimization and preparation of NAR-Soy protein complex (NAR-Sp) led to the optimum ratio of their interaction using Fourier Transform-Infrared spectroscopy (FT-IR) as the first level and layer of the formulation. The second layer of the formulation was to incorporate the NAR-Sp complex in aqueous-based gel-forming. The most optimum nanosuspension was determined using the gel sedimentation, sustained-release, pH-selective and targeted system. The most optimum components combinations and complex were characterized using different characterization tools, such as, the particle size analysis, SEM, TEM, PXRD and FT-IR. In addition, the optimum nanosuspension was characterized for its nanoparticle sensitivity against colorectal cancer cells using MTT assay in comparison to the untreated, naringenin, and blank groups. The complex enhanced the NAR's dissolution. The complex incorporation in the optimum nano-encapsulating system was characterized by the sustained-release and pH-selective behaviors to target the NAR release at the site of action or absorption. Interestingly, the optimum nano-encapsulating system was showing better colorectal cytotoxicity results in comparison to the other groups.
    MeSH term(s) Colorectal Neoplasms/drug therapy ; Delayed-Action Preparations ; Flavanones ; Humans ; Hydrogels ; Hydrogen-Ion Concentration ; Nanoparticles/chemistry ; Particle Size ; Polysaccharides ; Solubility ; Soybean Proteins ; Spectroscopy, Fourier Transform Infrared/methods ; Water
    Chemical Substances Delayed-Action Preparations ; Flavanones ; Hydrogels ; Polysaccharides ; Soybean Proteins ; Water (059QF0KO0R) ; naringenin (HN5425SBF2)
    Language English
    Publishing date 2022-08-22
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3151-3
    ISSN 1520-6017 ; 0022-3549
    ISSN (online) 1520-6017
    ISSN 0022-3549
    DOI 10.1016/j.xphs.2022.08.019
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  7. Article ; Online: Recent development of aptamer conjugated chitosan nanoparticles as cancer therapeutics.

    Sheikh, Afsana / Md, Shadab / Alhakamy, Nabil A / Kesharwani, Prashant

    International journal of pharmaceutics

    2022  Volume 620, Page(s) 121751

    Abstract: There is a surge in demand for safe and targeted therapy against cancer as the conventional treatment approach fails to reach the specific site. Chemotherapeutic agents are generally associated with low tumoral accumulation, off-site effect, and drug ... ...

    Abstract There is a surge in demand for safe and targeted therapy against cancer as the conventional treatment approach fails to reach the specific site. Chemotherapeutic agents are generally associated with low tumoral accumulation, off-site effect, and drug resistance. Targeted delivery with the use of nanocarrier could elevate the drug accumulation at the target site, reduce toxicity to non-cancerous cells, overcome drug resistance, and reduce dosing. Aptamers are single-stranded oligonucleotide that folds in a way to get into the pocket of target cells with high affinity and specificity due to the ability to recognize and interact with the biomarkers such as nucleolin, Mucin, EGFR, etc. overexpressed by cancer cells. Aptamer also plays a key role in cancer immunotherapy and the delivery of anti-cancer agents. The review brings the light upon the use of aptamer-chitosan nanoparticles against cancer therapy and their role in the reduction of toxic effects.
    MeSH term(s) Antineoplastic Agents ; Aptamers, Nucleotide ; Chitosan/therapeutic use ; Drug Delivery Systems ; Humans ; Nanoparticles ; Neoplasms/drug therapy
    Chemical Substances Antineoplastic Agents ; Aptamers, Nucleotide ; Chitosan (9012-76-4)
    Language English
    Publishing date 2022-04-15
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 428962-6
    ISSN 1873-3476 ; 0378-5173
    ISSN (online) 1873-3476
    ISSN 0378-5173
    DOI 10.1016/j.ijpharm.2022.121751
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  8. Article ; Online: Aptamer grafted nanoparticle as targeted therapeutic tool for the treatment of breast cancer.

    Sheikh, Afsana / Md, Shadab / Kesharwani, Prashant

    Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

    2021  Volume 146, Page(s) 112530

    Abstract: Breast carcinomas repeat their number and grow exponentially making it extremely frequent malignancy among women. Approximately, 70-80% of early diagnosed or non-metastatic conditions are treatable while the metastatic cases are considered ineffective to ...

    Abstract Breast carcinomas repeat their number and grow exponentially making it extremely frequent malignancy among women. Approximately, 70-80% of early diagnosed or non-metastatic conditions are treatable while the metastatic cases are considered ineffective to treat with current ample amount of therapy. Target based anti-cancer treatment has been in the limelight for decades and is perceived significant consideration of scientists. Aptamers are the 'coming of age' therapeutic approach, selected using an appropriate tool from the library of sequences. Aptamers are non-immunogenic, stable, and high-affinity ligand which are poised to reach the clinical benchmark. With the heed in nanoparticle application, the delivery of aptamer to the specific site could be enhanced which also protects them from nuclease degradation. Moreover, nanoparticles due to robust structure, high drug entrapment, and modifiable release of cargo could serve as a successful candidate in the treatment of breast carcinoma. This review would showcase the method and modified method of selection of aptamers, aptamers that were able to make its way towards clinical trial and their targetability and selectivity towards breast cancers. The appropriate usage of aptamer-based biosensor in breast cancer diagnosis have also been discussed.
    MeSH term(s) Aptamers, Nucleotide/chemistry ; Breast Neoplasms/diagnosis ; Breast Neoplasms/drug therapy ; Drug Delivery Systems ; Female ; Humans ; Nanoparticles ; SELEX Aptamer Technique
    Chemical Substances Aptamers, Nucleotide
    Language English
    Publishing date 2021-12-13
    Publishing country France
    Document type Journal Article ; Review
    ZDB-ID 392415-4
    ISSN 1950-6007 ; 0753-3322 ; 0300-0893
    ISSN (online) 1950-6007
    ISSN 0753-3322 ; 0300-0893
    DOI 10.1016/j.biopha.2021.112530
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  9. Article ; Online: The SH-SY5Y cell line: a valuable tool for Parkinson's disease drug discovery.

    Pandey, Manisha / Karmakar, Varnita / Majie, Ankit / Dwivedi, Monika / Md, Shadab / Gorain, Bapi

    Expert opinion on drug discovery

    2023  Volume 19, Issue 3, Page(s) 303–316

    Abstract: Introduction: Owing to limited efficient treatment strategies for highly prevalent and distressing Parkinson's disease (PD), an impending need emerged for deciphering new modes and mechanisms for effective management. SH-SY5Y-based : Areas covered: ... ...

    Abstract Introduction: Owing to limited efficient treatment strategies for highly prevalent and distressing Parkinson's disease (PD), an impending need emerged for deciphering new modes and mechanisms for effective management. SH-SY5Y-based
    Areas covered: Herein, the authors review the SH-SY5Y cell line and its value to PD research. The authors also provide the reader with their expert perspectives on how these developments can lead to the development of new impactful therapeutics.
    Expert opinion: Encouraged by recent research on SH-SY5Y cell lines, it was envisaged that this
    MeSH term(s) Humans ; Cell Line, Tumor ; Parkinson Disease/drug therapy ; Reproducibility of Results ; Neuroblastoma/metabolism ; Neuroblastoma/pathology ; Drug Discovery
    Language English
    Publishing date 2023-12-19
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2259618-5
    ISSN 1746-045X ; 1746-0441
    ISSN (online) 1746-045X
    ISSN 1746-0441
    DOI 10.1080/17460441.2023.2293158
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  10. Article ; Online: RGD engineered dendrimer nanotherapeutic as an emerging targeted approach in cancer therapy.

    Sheikh, Afsana / Md, Shadab / Kesharwani, Prashant

    Journal of controlled release : official journal of the Controlled Release Society

    2021  Volume 340, Page(s) 221–242

    Abstract: A bird's eye view is now demanded in the area of cancer research to suppress the suffering of cancer patient and mediate the lack of treatment related to chemotherapy. Chemotherapy is always preferred over surgery or radiation therapy, but they never met ...

    Abstract A bird's eye view is now demanded in the area of cancer research to suppress the suffering of cancer patient and mediate the lack of treatment related to chemotherapy. Chemotherapy is always preferred over surgery or radiation therapy, but they never met the patient's demand of safe medication. Targeted therapy has now been in research that could hinder the unnecessary effect of drug on normal cells but could affect the tumor cells in much efficient manner. Angiogenesis is process involved in development of new blood vessel that nourishes tumor growth. Integrin receptors are over expressed on cancer cells that play vital role in angiogenesis for growth and metastasis of tumor cell. A delivery of RGD based peptide to integrin targeted site could help in its successful binding and liberation of drug in tumor vasculature. Dendrimers, in addition to its excellent pharmacokinetic properties also helps to carry targeting ligand to site of tumor by successfully conjugating with them. The aim of this review is to bring light upon the role of integrin in cancer progression, interaction of RGD to integrin receptor and more importantly the RGD-dendrimer based targeted therapy for the treatment of various cancers.
    MeSH term(s) Dendrimers ; Humans ; Nanomedicine ; Neoplasms/drug therapy ; Neovascularization, Pathologic/drug therapy ; Oligopeptides
    Chemical Substances Dendrimers ; Oligopeptides ; arginyl-glycyl-aspartic acid (78VO7F77PN)
    Language English
    Publishing date 2021-10-29
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 632533-6
    ISSN 1873-4995 ; 0168-3659
    ISSN (online) 1873-4995
    ISSN 0168-3659
    DOI 10.1016/j.jconrel.2021.10.028
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