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  1. Article ; Online: Tuberculosis – burden and serodiagnosis

    Md. Mohiuddin

    IMC Journal of Medical Science, Vol 14, Iss 1, Pp 1-

    2020  Volume 11

    Abstract: Tuberculosis (TB) is one of the leading causes of death worldwide. Clinical features and demonstration of the organism by microscopy/culture are still the mainstay of diagnosis of tuberculosis. The present paper reviews the burden of TB and the role of ... ...

    Abstract Tuberculosis (TB) is one of the leading causes of death worldwide. Clinical features and demonstration of the organism by microscopy/culture are still the mainstay of diagnosis of tuberculosis. The present paper reviews the burden of TB and the role of serology in its diagnosis. IMC J Med Sci 2020; 14(1): 008. EPub date: 26 April 2020 Correspondence: Md. Mohiuddin, Department of Microbiology, Ibrahim Medical College, 1/A Ibrahim Sarani, Segunbagicha, Dhaka, Bangladesh, Email: mohicmc@gmail.com
    Keywords Medicine ; R
    Language English
    Publishing date 2020-06-01T00:00:00Z
    Publisher Ibrahim Medical College
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Cancer metastasis may increase COVID-19 mortality

    Md Mohiuddin / Kazuo Kasahara

    Journal of Infection and Public Health, Vol 15, Iss 2, Pp 153-

    Suitable targets required to impede cancer metastasis

    2022  Volume 155

    Keywords Infectious and parasitic diseases ; RC109-216 ; Public aspects of medicine ; RA1-1270
    Language English
    Publishing date 2022-02-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Complete genome sequence of the pandrug-resistant

    Kabir, Md Mohiuddin / Imam, Md Rayhan / Farzana, Zinat / Hossain, Chowdhury Faiz

    Microbiology resource announcements

    2023  Volume 12, Issue 12, Page(s) e0057723

    Abstract: ... Vibrio ... ...

    Abstract Vibrio cholerae
    Language English
    Publishing date 2023-11-15
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/MRA.00577-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Pedigree Analysis of Polycystic Kidney Disease Patients: Bangladeshi Perspective.

    Akther, Zohora / Amee, Samira Sultana / Masum, Md Mohiuddin / Nishat, Latifa / Yesmin, Zinnat Ara

    Indian journal of nephrology

    2023  Volume 33, Issue 6, Page(s) 440–443

    Abstract: Background: Polycystic kidney disease (PKD), an inheritance disorder which is the fourth leading cause of the end-stage renal disease. The inheritance pattern can be diagnosed and confirmed by pedigree analysis. The aim of the present research was to ... ...

    Abstract Background: Polycystic kidney disease (PKD), an inheritance disorder which is the fourth leading cause of the end-stage renal disease. The inheritance pattern can be diagnosed and confirmed by pedigree analysis. The aim of the present research was to determine the type and frequency of PKD using pedigree analysis.
    Materials and methods: The present research was designed as a cross-sectional descriptive study. Thirty-eight adult Bangladeshi PKD patients were recruited using a selection checklist from the Department of Nephrology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh. Data were collected using a data collection sheet after taking informed written consent. The pedigree was drawn using the genetic pedigree chart creation software f-tree V4.0.6. The percentage frequencies of different types of pedigree were calculated using the Statistical Package for the Social Sciences software, version 23.
    Results: A total of 24 (63.20%) had a positive family history and 36.80% (14) had no positive family history. All of the patients with a positive family history had vertical transmission; male and female were equally inheriting the gene. Out of these 24 patients, 4.17% (one), 8.33% (two), and 16.67% (four) had a homozygous/heterozygous state, skip generation, and male to male transmission, respectively.
    Conclusions: Pedigree analysis of PKD patients showed an increased value in early diagnosis and better management and prognosis of the disease.
    Language English
    Publishing date 2023-05-03
    Publishing country India
    Document type Journal Article
    ZDB-ID 2134388-3
    ISSN 1998-3662 ; 0971-4065
    ISSN (online) 1998-3662
    ISSN 0971-4065
    DOI 10.4103/ijn.ijn_234_22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Real Time PCR-based diagnosis of human visceral leishmaniasis using urine samples.

    Samiur Rahim / Md Mohiuddin Sharif / Md Robed Amin / Mohammad Tariqur Rahman / Muhammad Manjurul Karim

    PLOS Global Public Health, Vol 2, Iss 12, p e

    2022  Volume 0000834

    Abstract: Diagnosis of visceral leishmaniasis (VL) through the detection of its causative agents namely Leishmania donovani and L. infantum is traditionally based on immunochromatographic tests, microscopy of bone marrow, spleen aspirates, liver or lymph node and ... ...

    Abstract Diagnosis of visceral leishmaniasis (VL) through the detection of its causative agents namely Leishmania donovani and L. infantum is traditionally based on immunochromatographic tests, microscopy of bone marrow, spleen aspirates, liver or lymph node and differential diagnosis. While the first process has low specificity, the later one carries the risk of fatal hemorrhage. Over the last decade, multiple Polymerase Chain Reaction (PCR) based diagnosis has been developed using blood and urine sample with a varying degree of sensitivity and specificity, an issue worth improving for precision diagnosis. Earlier, we reported a PCR-based diagnosis of L. donovani in peripheral blood using a novel set of PCR primers with absolute specificity. Using the same set of primers and PCR conditions, here we describe diagnosis of L. donovani from urine, for a non-invasive, rapid and safe diagnosis. Diagnosis of VL was carried out using urine samples collected from clinically diagnosed VL patients (n = 23) of Bangladesh in Real Time PCR. Test results were validated by comparing blood samples from the same set of patients. Sensitivity and specificity of this diagnosis was analyzed using retrospective bone marrow samples, collected earlier from confirmed VL patients (n = 19). The method showed 100% sensitivity in detecting L. donovani in urine and corresponding blood and retrospective bone marrow samples, as well as 100% specificity in control groups. A Real Time PCR-based molecular detection system using urine sample is hereafter presented what could be a, non-invasive approach for VL detection with precision and perfection.
    Keywords Public aspects of medicine ; RA1-1270
    Subject code 610
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Impact of COVID-19 pandemic on the physical, mental and social health of the suburban and rural adult population in Bangladesh

    Nehlin Tomalika / Rishad Mahzabeen / Md Mohiuddin Tagar / Sadya Afroz / Naima Ahmed / Masuda Mohsena / Rashid-E-Mahbub / MA Sayeed

    IMC Journal of Medical Science, Vol 18, Iss 1, Pp 1-

    2023  Volume 5

    Abstract: Background and objectives: The COVID-19 pandemic caused a significant impact on health worldwide. Adverse effect of COVID-19 on health-related quality of life is significant. This study aimed to find out the impact of COVID-19 on the physical, mental and ...

    Abstract Background and objectives: The COVID-19 pandemic caused a significant impact on health worldwide. Adverse effect of COVID-19 on health-related quality of life is significant. This study aimed to find out the impact of COVID-19 on the physical, mental and social health of suburban and rural adult population in Bangladesh. Methods: A suburban and a rural community were purposively selected. The suburban and rural areas were located about 40 km and 130 km north and north-east of Dhaka city respectively. People aged ≥20 years in the selected communities were enrolled in the study. The investigation procedure included socio-demographic and clinical history, anthropometry, and clinical examination and laboratory investigations. Depression, Anxiety and Stress Scale-21 (DASS-21) and 36-Item Short Form Health Survey (SF-36) questionnaires were used for assessing mental and social health respectively. Knowledge, attitude and practice (KAP) regarding the prevention and transmission of COVID-19 was assessed by a validated questionnaire and interview. Results: Total 385 individuals (suburban=201, rural=184) were enrolled in the study. Out of 385, 116 and 269 were male and female, respectively. Out of total 385 participants, depression, anxiety and stress were present in 113 (29.4%), 144 (37.4%) and 70 (18.2%) respectively, while 210 (54.5%) were normal. Extremely severe depression, anxiety and stress were present in 3.6%, 6% and 0.5%, respectively. Depression and anxiety did not differ between suburban and rural populations, though stress was significantly higher among the suburban (p<0.05) population. Social functioning was limited in more than 50% as opposed to excellent (5.5%) or good (39.8%). Almost 60% of the participants had to cut-down schedule of heavy work. Moderate to minimal physical activities were less affected, though weakness and nervousness predominantly hindered socialization. About the prevention and transmission of COVID-19, awareness and attitude were found satisfactory (≥45%), though practice was ...
    Keywords Medicine ; R
    Subject code 360
    Language English
    Publishing date 2023-12-01T00:00:00Z
    Publisher Ibrahim Medical College
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Einfluss der Wirtresistenz auf die Aggressivität von Exserohilum turcicum an Mais

    Ludwig Navarro, Barbara / Mithon, Md Mohiuddin / Savory, Musrat / Tiedemann, Andreas von

    2023  

    Keywords Text ; abstract_or_summary ; ddc:630
    Language German
    Publisher Julius Kühn-Institut
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Real Time PCR-based diagnosis of human visceral leishmaniasis using urine samples.

    Rahim, Samiur / Sharif, Md Mohiuddin / Amin, Md Robed / Rahman, Mohammad Tariqur / Karim, Muhammad Manjurul

    PLOS global public health

    2022  Volume 2, Issue 12, Page(s) e0000834

    Abstract: Diagnosis of visceral leishmaniasis (VL) through the detection of its causative agents namely Leishmania donovani and L. infantum is traditionally based on immunochromatographic tests, microscopy of bone marrow, spleen aspirates, liver or lymph node and ... ...

    Abstract Diagnosis of visceral leishmaniasis (VL) through the detection of its causative agents namely Leishmania donovani and L. infantum is traditionally based on immunochromatographic tests, microscopy of bone marrow, spleen aspirates, liver or lymph node and differential diagnosis. While the first process has low specificity, the later one carries the risk of fatal hemorrhage. Over the last decade, multiple Polymerase Chain Reaction (PCR) based diagnosis has been developed using blood and urine sample with a varying degree of sensitivity and specificity, an issue worth improving for precision diagnosis. Earlier, we reported a PCR-based diagnosis of L. donovani in peripheral blood using a novel set of PCR primers with absolute specificity. Using the same set of primers and PCR conditions, here we describe diagnosis of L. donovani from urine, for a non-invasive, rapid and safe diagnosis. Diagnosis of VL was carried out using urine samples collected from clinically diagnosed VL patients (n = 23) of Bangladesh in Real Time PCR. Test results were validated by comparing blood samples from the same set of patients. Sensitivity and specificity of this diagnosis was analyzed using retrospective bone marrow samples, collected earlier from confirmed VL patients (n = 19). The method showed 100% sensitivity in detecting L. donovani in urine and corresponding blood and retrospective bone marrow samples, as well as 100% specificity in control groups. A Real Time PCR-based molecular detection system using urine sample is hereafter presented what could be a, non-invasive approach for VL detection with precision and perfection.
    Language English
    Publishing date 2022-12-29
    Publishing country United States
    Document type Journal Article
    ISSN 2767-3375
    ISSN (online) 2767-3375
    DOI 10.1371/journal.pgph.0000834
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Humoral immune response to selective mycobacterial antigens and serodiagnosis of active tuberculosis in Bangladeshi patients

    Md. Mohiuddin / J Ashraful Haq

    IMC Journal of Medical Science, Vol 10, Iss 2, Pp 53-

    2016  Volume 57

    Abstract: Background and objective:Serological test has become an important tool in the diagnosis of TB cases. This study focused on the analysis and comparison of antibody response to two Mycobacterium tuberculosis (MTB) antigens namely Ag85 complex and culture ... ...

    Abstract Background and objective:Serological test has become an important tool in the diagnosis of TB cases. This study focused on the analysis and comparison of antibody response to two Mycobacterium tuberculosis (MTB) antigens namely Ag85 complex and culture filtrate protein (CFP) in patients with tuberculosis. Antibody response to specific antigen was utilized as a diagnostic tool to detect active tuberculosis (TB) cases. Methods: Sera from 30 patients with active tuberculosis and 30 healthy individuals were tested by enzyme linked immunosorbent assay (ELISA) for the presence of immunoglobulin (Ig) G and IgM antibodies against Ag85 complex and culture filtrate protein (CFP) antigens of MTB. Results:The mean OD values of serum IgM and IgG antibodies against Ag85 and CFP were significantly (p<0.0001) higher in patients than that of healthy control individuals. Among the 30 tuberculosis patients, anti-Ag85 complex IgM and IgG was positive in 66.7% and 70.0% patients respectively. The seropositive rate of anti-CFP IgM and IgG was 33.3% and 56.7% respectively. The sensitivity and specificity of anti Ag85 and anti-CFP IgM and IgG ranged from 60.0% to 96.7%. Conclusion: The study demonstrated that determination of IgM and IgG antibodies against Ag85 complex and CFP could be used as a serological marker for diagnosis of active tuberculosis. IMC J Med Sci 2016; 10(2): 53-57
    Keywords Medicine ; R
    Subject code 616
    Language English
    Publishing date 2016-07-01T00:00:00Z
    Publisher Ibrahim Medical College
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Humoral immune response to Mycobactrium tuberculosis cell wall fraction and lipoarabinomannan antigens in Bangladeshi patients with active tuberculosis

    Md. Mohiuddin / J Ashraful Haq

    IMC Journal of Medical Science, Vol 10, Iss 2, Pp 58-

    2016  Volume 60

    Abstract: Background and objective: This study focused on the analysis and comparison of humoral immune response to Mycobacterium tuberculosis (MTB) cell wall fraction (CWF) and lipoarabinomannan (LAM) antigens. Methods: Sera from adult patients with active ... ...

    Abstract Background and objective: This study focused on the analysis and comparison of humoral immune response to Mycobacterium tuberculosis (MTB) cell wall fraction (CWF) and lipoarabinomannan (LAM) antigens. Methods: Sera from adult patients with active pulmonary tuberculosis and age and sex matched healthy individuals were tested for immunoglobulin (Ig) M and IgG antibodies to CWF and LAM by enzyme linked immunosorbent assay (ELISA). Results: The mean OD values of serum IgM and IgG antibodies against CWF of TB patients was not significantly (p=0.52, p=0.45) different from that of healthy control population. However, the mean OD values of serum IgM and IgG against LAM were significantly (p=0.049, p= 0.001) higher in TB cases than that of healthy individuals. Conclusion: The present study has revealed that IgM and IgG antibody to LAM may be used in serodiagnosis of TB while response to CWF in active TB case is restricted in our population. IMC J Med Sci 2016; 10(2): 58-60
    Keywords Medicine ; R
    Subject code 616
    Language English
    Publishing date 2016-07-01T00:00:00Z
    Publisher Ibrahim Medical College
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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