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Book ; Online: Functional Imaging in living Plants - Cell Biology meets Physiology

Meckel, Tobias / Littlejohn, George R. / Schwarzlander, Markus / Costa, Alex

2015

Abstract: The study of plant cell physiology is currently experiencing a profound transformation. Novel techniques allow dynamic in vivo imaging with subcellular resolution, covering a rapidly growing range of plant cell physiology. Several basic biological ... ...

Abstract	The study of plant cell physiology is currently experiencing a profound transformation. Novel techniques allow dynamic in vivo imaging with subcellular resolution, covering a rapidly growing range of plant cell physiology. Several basic biological questions that have been inaccessible by the traditional combination of biochemical, physiological and cell biological approaches now see major progress. Instead of grinding up tissues, destroying their organisation, or describing cell- and tissue structure, without a measure for its function, novel imaging approaches can provide the critical link between localisation, function and dynamics.- Thanks to a fast growing collection of available fluorescent protein variants and sensors, along with innovative new microscopy technologies and quantitative analysis tools, a wide range of plant biology can now be studied in vivo, including cell morphology & migration, protein localization, topology & movement, protein-protein interaction, organelle dynamics, as well as ion, ROS & redox dynamics. Within the cell, genetic targeting of fluorescent protein probes to different organelles and subcellular locations has started to reveal the stringently compartmentalized nature of cell physiology and its sophisticated spatiotemporal regulation in response to environmental stimuli. Most importantly, such cellular processes can be monitored in their natural 3D context, even in complex tissues and organs - a condition not easily met in studies on mammalian cells.- Recent new insights into plant cell physiology by functional imaging have been largely driven by technological developments, such as the design of novel sensors, innovative microscopy & imaging techniques and the quantitative analysis of complex image data. Rapid further advances are expected which will require close interdisciplinary interaction of plant biologists with chemists, physicists, mathematicians and computer scientists. High-throughput approaches will become increasingly important, to fill genomic data with 'life' on the scale of cell physiology. If the vast body of information generated in the -omics era is to generate actual mechanistic understanding of how the live plant cell works, functional imaging has enormous potential to adopt the role of a versatile standard tool across plant biology and crop breeding.-
Keywords	Botany ; Science (General)
Size	1 electronic resource (114 p.)
Publisher	Frontiers Media SA
Document type	Book ; Online
Note	English ; Open Access
HBZ-ID	HT020091034
ISBN	9782889194650 ; 2889194655
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article ; Online: Isotachophoresis with Oscillating Sample Zones to Control the Spatial Overlap of Co-focused Species.

Gebhard, Florian / Bonart, Henning / Roy, Tamal / Meckel, Tobias / Hardt, Steffen

Analytical chemistry

2024 Volume 96, Issue 11, Page(s) 4446–4454

Abstract: Microfluidic isotachophoresis (ITP) is a powerful technique that can significantly increase the reaction rate of homogeneous chemical reactions by cofocusing reactants in a narrow sample zone. Correspondingly, ITP has been utilized to reduce the reaction ...

Abstract	Microfluidic isotachophoresis (ITP) is a powerful technique that can significantly increase the reaction rate of homogeneous chemical reactions by cofocusing reactants in a narrow sample zone. Correspondingly, ITP has been utilized to reduce the reaction time in various bioanalytical assays. However, in conventional ITP, it is hardly possible to control the reaction rate in real time, i.e., speeding up or slowing down a reaction on demand. Here, we experimentally demonstrate a new mode of ITP that allows the spatial overlap of two ITP zones to be precisely controlled over time, which is a crucial first step toward controlling reaction rates. Two nonreactive samples are initially focused and separated by a spacer using a DC electric field. By superimposing an oscillating field component with sufficiently high amplitude on the DC field, the spatial overlap of their concentration profiles is temporarily increased due to electromigration dispersion. The time-average of this overlap can be precisely controlled by varying the frequency and amplitude of the oscillation. We suggest that this scheme can be transferred to chemical reactions between ionic species with sufficiently different electrophoretic mobilities. Tuning the parameters of the oscillatory electric field should allow direct control of the corresponding reaction rate.
Language	English
Publishing date	2024-03-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.3c04606
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Chemical Gradients in Polymer-Modified Paper Sheets-Towards Single-Layer Biomimetic Soft Robots.

Schäfer, Jan-Lukas / Meckel, Tobias / Poppinga, Simon / Biesalski, Markus

Biomimetics (Basel, Switzerland)

2023 Volume 8, Issue 1

Abstract: Biomimetic actuators are typically constructed as functional bi- or multilayers, where actuating and resistance layers together dictate bending responses upon triggering by environmental stimuli. Inspired by motile plant structures, like the stems of the ...

Abstract	Biomimetic actuators are typically constructed as functional bi- or multilayers, where actuating and resistance layers together dictate bending responses upon triggering by environmental stimuli. Inspired by motile plant structures, like the stems of the false rose of Jericho (
Language	English
Publishing date	2023-01-18
Publishing country	Switzerland
Document type	Journal Article
ISSN	2313-7673
ISSN (online)	2313-7673
DOI	10.3390/biomimetics8010043
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Porosity Centrifuge: Determination of Pore Sizes of Swellable Porous Materials under Hypergravity

Postulka, Niels / Meckel, Tobias / Biesalski, Markus

Langmuir. 2021 July 16, v. 37, no. 29

2021

Abstract: Porous materials are ubiquitous and essential for many processes in nature as well as in industry, and the need to produce them from renewable materials will definitely increase. A prominent example for such a fully recyclable and biogenic porous ... ...

Abstract	Porous materials are ubiquitous and essential for many processes in nature as well as in industry, and the need to produce them from renewable materials will definitely increase. A prominent example for such a fully recyclable and biogenic porous material is paper, a material that contains macropores formed in between the fibers as well as a large distribution of much finer pores on and within the fiber walls. While the determination of pore sizes is of central importance for the characterization of such materials, their determination is usually only possible with complex methodologies. The determination of pore sizes in the context of water has remained largely unsolved to date, in particular, if water-swellable materials are considered. Here, we introduce a completely new way of determining pore sizes of materials even under swelling conditions. Using a centrifugal device and studying the imbibition of water into paper at various centrifugal forces that oppose the capillary forces, we can access the mean pore size of different paper materials in an experimentally simple fashion. In addition, we can show that the pore size values obtained with our “centrifugal porosimetry” are consistent with the values obtained using other methods, usually much more involved methods. For this purpose, we measure well-characterized translucent macroporous materials using water, ranging from simple glass capillaries to standard filters and nitrocellulose membranes.
Keywords	glass ; imbibition ; industry ; macropores ; paper ; porosity ; porous media
Language	English
Dates of publication	2021-0716
Size	p. 8746-8752.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	2005937-1
ISSN	1520-5827 ; 0743-7463
ISSN (online)	1520-5827
ISSN	0743-7463
DOI	10.1021/acs.langmuir.1c01002
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Porosity Centrifuge: Determination of Pore Sizes of Swellable Porous Materials under Hypergravity.

Postulka, Niels / Meckel, Tobias / Biesalski, Markus

Langmuir : the ACS journal of surfaces and colloids

2021 Volume 37, Issue 29, Page(s) 8746–8752

Abstract	Porous materials are ubiquitous and essential for many processes in nature as well as in industry, and the need to produce them from renewable materials will definitely increase. A prominent example for such a fully recyclable and biogenic porous material is paper, a material that contains macropores formed in between the fibers as well as a large distribution of much finer pores on and within the fiber walls. While the determination of pore sizes is of central importance for the characterization of such materials, their determination is usually only possible with complex methodologies. The determination of pore sizes in the context of water has remained largely unsolved to date, in particular, if water-swellable materials are considered. Here, we introduce a completely new way of determining pore sizes of materials even under swelling conditions. Using a centrifugal device and studying the imbibition of water into paper at various centrifugal forces that oppose the capillary forces, we can access the mean pore size of different paper materials in an experimentally simple fashion. In addition, we can show that the pore size values obtained with our "centrifugal porosimetry" are consistent with the values obtained using other methods, usually much more involved methods. For this purpose, we measure well-characterized translucent macroporous materials using water, ranging from simple glass capillaries to standard filters and nitrocellulose membranes.
Language	English
Publishing date	2021-07-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	2005937-1
ISSN	1520-5827 ; 0743-7463
ISSN (online)	1520-5827
ISSN	0743-7463
DOI	10.1021/acs.langmuir.1c01002
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Entrapment of Hydrophobic Biocides into Cellulose Acetate Nanoparticles by Nanoprecipitation.

Cordt, Cynthia / Meckel, Tobias / Geissler, Andreas / Biesalski, Markus

Nanomaterials (Basel, Switzerland)

2020 Volume 10, Issue 12

Abstract: This contribution reports an efficient method for the production and use of biocide-loaded cellulose acetate nanoparticles. As well-known model biocides 4-Hexylresorcinol and Triclosan were used for in situ nanoparticle loading during a nanoprecipitation ...

Abstract	This contribution reports an efficient method for the production and use of biocide-loaded cellulose acetate nanoparticles. As well-known model biocides 4-Hexylresorcinol and Triclosan were used for in situ nanoparticle loading during a nanoprecipitation process. We show that the nanoparticle size can be well-controlled by variation of the cellulose acetate concentration during nanoprecipitation. Apart from strong evidence suggesting cellulose acetate particle formation according to a nucleation-aggregation mechanism, we further show that the biocide loading of the particles occurs by a diffusion process and not via co-precipitation. The quantity of particle loading was analyzed by
Language	English
Publishing date	2020-12-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662255-5
ISSN	2079-4991
ISSN	2079-4991
DOI	10.3390/nano10122447
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Diazo-Based Copolymers for the Wet Strength Improvement of Paper Based on Thermally Induced CH-Insertion Cross-Linking

Schölch, Simon / Schäfer, Jan-Lukas / Meckel, Tobias / Brandstetter, Thomas / Biesalski, Markus / Rühe, Jürgen

Biomacromolecules. 2021 June 14, v. 22, no. 7

2021

Abstract: We present an alternative to commonly used, but from an environmental point of view, problematic wet strength agents, which are usually added to paper to prevent a loss of mechanical stability and finally disintegrate when they get into contact with ... ...

Abstract	We present an alternative to commonly used, but from an environmental point of view, problematic wet strength agents, which are usually added to paper to prevent a loss of mechanical stability and finally disintegrate when they get into contact with water. To this end, diazoester-containing copolymers are generated, which are coated onto paper and by heating to 110–160 °C for short periods of time become activated and form carbene intermediates, which undergo a CH-insertion cross-linking reaction. The process leads to a simultaneous cross-linking of the polymer and its attachment to the cellulose substrate. The immobilization process of copolymers consisting of a hydrophilic matrix based on N,N-dimethylacrylamide and a diazoester-based comonomer to a cellulose model surface and to laboratory-engineered, fibrous paper substrates is investigated as a function of time, temperature, and cross-linker composition. The distribution of the polymer in the fiber network is studied using confocal fluorescence microscopy. Finally, the tensile properties of modified wet and dry eucalyptus sulfate papers are measured to demonstrate the strong effect of the thermally cross-linked copolymers on the wet strength of paper substrates. Initial experiments show that the tensile indices of the modified and wetted paper samples are up to 50 times higher compared to the values measured for unmodified samples. When dry and wet papers coated with the above-described wetting agents are compared, relative wet strengths of over 30% are observed.
Keywords	Eucalyptus ; carbenes ; cellulose ; composite polymers ; crosslinking ; fluorescence microscopy ; hydrophilicity ; paper ; sulfates ; temperature ; wet strength
Language	English
Dates of publication	2021-0614
Size	p. 2864-2873.
Publishing place	American Chemical Society
Document type	Article
ISSN	1526-4602
DOI	10.1021/acs.biomac.1c00249
Database	NAL-Catalogue (AGRICOLA)

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Article: Humidity influence on mechanics of paper materials: joint numerical and experimental study on fiber and fiber network scale

Lin, Binbin / Auernhammer, Julia / Schäfer, Jan-Lukas / Meckel, Tobias / Stark, Robert / Biesalski, Markus / Xu, Bai-Xiang

Cellulose. 2022 Jan., v. 29, no. 2

2022

Abstract: Paper materials are well-known to be hydrophilic unless chemical and mechanical processing treatments are undertaken. The relative humidity impacts the fiber elasticity, the interfiber joint behavior and the failure mechanism. In this work, we present a ... ...

Abstract	Paper materials are well-known to be hydrophilic unless chemical and mechanical processing treatments are undertaken. The relative humidity impacts the fiber elasticity, the interfiber joint behavior and the failure mechanism. In this work, we present a comprehensive experimental and computational study on mechanical properties of the fiber and the fiber network under humidity influence. The manually extracted cellulose fiber is exposed to different levels of humidity, and then mechanically characterized using atomic force microscopy, which delivers the humidity dependent longitudinal Young’s modulus. We describe the relation and calibrate the data into an exponential function, and the obtained relationship allows calculation of fiber elastic modulus at any humidity level. Moreover, by using confoncal laser scanning microscopy, the coefficient of hygroscopic expansion of the fibers is determined. We further present a finite element model to simulate the deformation and the failure of the fiber network. The model includes the fiber anisotropy and the hygroscopic expansion using the experimentally determined constants, and further considers interfiber behavior and debonding by using a humidity dependent cohesive zone interface model. Simulations on exemplary fiber network samples are performed to demonstrate the influence of different aspects including relative humidity and fiber-fiber bonding parameters on the mechanical features, such as force-elongation curve, strength and extensibility. Finally, we provide computational insights for interfiber bond damage pattern with respect to different humidity level as further outlook.
Keywords	anisotropy ; atomic force microscopy ; cellulose ; cellulosic fibers ; deformation ; extensibility ; finite element analysis ; hydrophilicity ; modulus of elasticity ; paper ; relative humidity ; simulation models
Language	English
Dates of publication	2022-01
Size	p. 1129-1148.
Publishing place	Springer Netherlands
Document type	Article
ZDB-ID	1496831-9
ISSN	1572-882X ; 0969-0239
ISSN (online)	1572-882X
ISSN	0969-0239
DOI	10.1007/s10570-021-04355-y
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Diazo-Based Copolymers for the Wet Strength Improvement of Paper Based on Thermally Induced CH-Insertion Cross-Linking.

Schölch, Simon / Schäfer, Jan-Lukas / Meckel, Tobias / Brandstetter, Thomas / Biesalski, Markus / Rühe, Jürgen

Biomacromolecules

2021 Volume 22, Issue 7, Page(s) 2864–2873

Abstract	We present an alternative to commonly used, but from an environmental point of view, problematic wet strength agents, which are usually added to paper to prevent a loss of mechanical stability and finally disintegrate when they get into contact with water. To this end, diazoester-containing copolymers are generated, which are coated onto paper and by heating to 110-160 °C for short periods of time become activated and form carbene intermediates, which undergo a CH-insertion cross-linking reaction. The process leads to a simultaneous cross-linking of the polymer and its attachment to the cellulose substrate. The immobilization process of copolymers consisting of a hydrophilic matrix based on
MeSH term(s)	Cellulose ; Hydrophobic and Hydrophilic Interactions ; Tensile Strength ; Water
Chemical Substances	Water (059QF0KO0R) ; Cellulose (9004-34-6)
Language	English
Publishing date	2021-06-14
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1526-4602
ISSN (online)	1526-4602
DOI	10.1021/acs.biomac.1c00249
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Design, synthesis and biological evaluation of novel aminopyrazole- and 7-azaindole-based Nek1 inhibitors and their effects on zebrafish kidney development.

Pilakowski, Johannes / Baumann, Georg / Shih, Yung-Hsin / Meckel, Tobias / Schmidt, Boris

Bioorganic & medicinal chemistry letters

2021 Volume 53, Page(s) 128418

Abstract: NIMA-related protein kinase Nek1 is crucially involved in cell cycle regulation, DNA repair and microtubule regulation and dysfunctions of Nek1 play key roles in amyotrophic lateral sclerosis (ALS), polycystic kidney disease (PKD) and several types of ... ...

Abstract	NIMA-related protein kinase Nek1 is crucially involved in cell cycle regulation, DNA repair and microtubule regulation and dysfunctions of Nek1 play key roles in amyotrophic lateral sclerosis (ALS), polycystic kidney disease (PKD) and several types of radiotherapy resistant cancer. Targeting of Nek1 could reveal a new class of radiosensitizing substances and provide useful tools to better understand the aforementioned diseases. In this report we explore substituted aminopyrazoles and 7-azaindoles as potent inhibitors for the Nek1 kinase domain and examine their effect on kidney organogenesis in Danio rerio.
MeSH term(s)	Animals ; Dose-Response Relationship, Drug ; Drug Design ; Indoles/chemical synthesis ; Indoles/chemistry ; Indoles/pharmacology ; Kidney/drug effects ; Kidney/growth & development ; Kidney/metabolism ; Molecular Structure ; NIMA-Related Kinase 1/antagonists & inhibitors ; NIMA-Related Kinase 1/metabolism ; Protein Kinase Inhibitors/chemical synthesis ; Protein Kinase Inhibitors/chemistry ; Protein Kinase Inhibitors/pharmacology ; Pyrazoles/chemical synthesis ; Pyrazoles/chemistry ; Pyrazoles/pharmacology ; Structure-Activity Relationship ; Zebrafish
Chemical Substances	7-azaindole dimer ; Indoles ; Protein Kinase Inhibitors ; Pyrazoles ; NIMA-Related Kinase 1 (EC 2.7.11.1)
Language	English
Publishing date	2021-10-26
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1063195-1
ISSN	1464-3405 ; 0960-894X
ISSN (online)	1464-3405
ISSN	0960-894X
DOI	10.1016/j.bmcl.2021.128418
Database	MEDical Literature Analysis and Retrieval System OnLINE

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